N-ethylmaleimide

1-ethylpyrrole-2,5-dione

N-Ethylmaleimide (NEM) is an organic compound that is derived from maleic acid. It contains the imide functional group, but more importantly it is an alkene that is reactive toward thiols and is commonly used to modify cysteine residues in proteins and peptides.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 

PubChem MeSH terms: Enzyme Inhibitors;Sulfhydryl Reagents



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_392
Screen concentration 8.3 μM
Source Miscellaneous
PubChem CID 4362
SMILES CCN1C(=O)C=CC1=O
Standardized SMILES CCN1C(=O)C=CC1=O
Molecular weight 125.1253
ALogP 0.16
H-bond donor count 0
H-bond acceptor count 2
Response signature RNA pol III & RNase P/MRP

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.82
% growth inhibition (Hom. pool) 13.06


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4362
Download HIP data (tab-delimited text)  (excel)
Gene:ADE13(YLR359W)|FD-Score:-3.27|P-value:5.39E-4|Clearance:0||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:DRS1(YLL008W)|FD-Score:4.21|P-value:1.26E-5|Clearance:0.05||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:ENP1(YBR247C)|FD-Score:4.44|P-value:4.43E-6|Clearance:0.1||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:ERB1(YMR049C)|FD-Score:3.28|P-value:5.17E-4|Clearance:0.18||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:GCD1(YOR260W)|FD-Score:11.5|P-value:5.74E-31|Clearance:5.03||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCD11(YER025W)|FD-Score:3.42|P-value:3.09E-4|Clearance:0.14||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GCD2(YGR083C)|FD-Score:14.6|P-value:2.71E-48|Clearance:5.03||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCD6(YDR211W)|FD-Score:6.48|P-value:4.65E-11|Clearance:0.93||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:GCD7(YLR291C)|FD-Score:16.7|P-value:6.59E-63|Clearance:5.03||SGD DESC:Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPI12(YMR281W)|FD-Score:-4.62|P-value:1.89E-6|Clearance:0||SGD DESC:ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp Gene:GRC3(YLL035W)|FD-Score:-4.01|P-value:3.10E-5|Clearance:0||SGD DESC:Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated Gene:HIP1(YGR191W)|FD-Score:3.58|P-value:1.70E-4|Clearance:0.04||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:KAP95(YLR347C)|FD-Score:3.55|P-value:1.96E-4|Clearance:0.12||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:KRR1(YCL059C)|FD-Score:-3.91|P-value:4.68E-5|Clearance:0||SGD DESC:Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit Gene:NSE4(YDL105W)|FD-Score:4.35|P-value:6.91E-6|Clearance:0.01||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:NUF2(YOL069W)|FD-Score:-3.26|P-value:5.49E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:ORC4(YPR162C)|FD-Score:-3.88|P-value:5.28E-5|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PRP21(YJL203W)|FD-Score:-3.48|P-value:2.53E-4|Clearance:0||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly Gene:QNS1(YHR074W)|FD-Score:-3.37|P-value:3.76E-4|Clearance:0||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RET1(YOR207C)|FD-Score:5.02|P-value:2.59E-7|Clearance:0.19||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RLP7(YNL002C)|FD-Score:3.72|P-value:9.89E-5|Clearance:0.09||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPC31(YNL151C)|FD-Score:3.89|P-value:5.08E-5|Clearance:0.16||SGD DESC:RNA polymerase III subunit C31 Gene:RPC53(YDL150W)|FD-Score:4.72|P-value:1.16E-6|Clearance:0.28||SGD DESC:RNA polymerase III subunit C53 Gene:RPO31(YOR116C)|FD-Score:4.33|P-value:7.36E-6|Clearance:0.12||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:SMC1(YFL008W)|FD-Score:3.63|P-value:1.39E-4|Clearance:0.05||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:SPP381(YBR152W)|FD-Score:3.1|P-value:9.61E-4|Clearance:0.05||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:SUI3(YPL237W)|FD-Score:4.16|P-value:1.60E-5|Clearance:0.27||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:YDL152W(YDL152W_d)|FD-Score:4.82|P-value:7.00E-7|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YPL238C(YPL238C_d)|FD-Score:5.54|P-value:1.47E-8|Clearance:0.53||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:ADE13(YLR359W)|FD-Score:-3.27|P-value:5.39E-4|Clearance:0||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:DRS1(YLL008W)|FD-Score:4.21|P-value:1.26E-5|Clearance:0.05||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:ENP1(YBR247C)|FD-Score:4.44|P-value:4.43E-6|Clearance:0.1||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:ERB1(YMR049C)|FD-Score:3.28|P-value:5.17E-4|Clearance:0.18||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:GCD1(YOR260W)|FD-Score:11.5|P-value:5.74E-31|Clearance:5.03||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCD11(YER025W)|FD-Score:3.42|P-value:3.09E-4|Clearance:0.14||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GCD2(YGR083C)|FD-Score:14.6|P-value:2.71E-48|Clearance:5.03||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCD6(YDR211W)|FD-Score:6.48|P-value:4.65E-11|Clearance:0.93||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:GCD7(YLR291C)|FD-Score:16.7|P-value:6.59E-63|Clearance:5.03||SGD DESC:Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPI12(YMR281W)|FD-Score:-4.62|P-value:1.89E-6|Clearance:0||SGD DESC:ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp Gene:GRC3(YLL035W)|FD-Score:-4.01|P-value:3.10E-5|Clearance:0||SGD DESC:Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated Gene:HIP1(YGR191W)|FD-Score:3.58|P-value:1.70E-4|Clearance:0.04||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:KAP95(YLR347C)|FD-Score:3.55|P-value:1.96E-4|Clearance:0.12||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:KRR1(YCL059C)|FD-Score:-3.91|P-value:4.68E-5|Clearance:0||SGD DESC:Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit Gene:NSE4(YDL105W)|FD-Score:4.35|P-value:6.91E-6|Clearance:0.01||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:NUF2(YOL069W)|FD-Score:-3.26|P-value:5.49E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:ORC4(YPR162C)|FD-Score:-3.88|P-value:5.28E-5|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PRP21(YJL203W)|FD-Score:-3.48|P-value:2.53E-4|Clearance:0||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly Gene:QNS1(YHR074W)|FD-Score:-3.37|P-value:3.76E-4|Clearance:0||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RET1(YOR207C)|FD-Score:5.02|P-value:2.59E-7|Clearance:0.19||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RLP7(YNL002C)|FD-Score:3.72|P-value:9.89E-5|Clearance:0.09||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPC31(YNL151C)|FD-Score:3.89|P-value:5.08E-5|Clearance:0.16||SGD DESC:RNA polymerase III subunit C31 Gene:RPC53(YDL150W)|FD-Score:4.72|P-value:1.16E-6|Clearance:0.28||SGD DESC:RNA polymerase III subunit C53 Gene:RPO31(YOR116C)|FD-Score:4.33|P-value:7.36E-6|Clearance:0.12||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:SMC1(YFL008W)|FD-Score:3.63|P-value:1.39E-4|Clearance:0.05||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:SPP381(YBR152W)|FD-Score:3.1|P-value:9.61E-4|Clearance:0.05||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:SUI3(YPL237W)|FD-Score:4.16|P-value:1.60E-5|Clearance:0.27||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:YDL152W(YDL152W_d)|FD-Score:4.82|P-value:7.00E-7|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YPL238C(YPL238C_d)|FD-Score:5.54|P-value:1.47E-8|Clearance:0.53||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4362
Download HOP data (tab-delimited text)  (excel)
Gene:ALB1(YJL122W)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p Gene:ARG5,6(YER069W)|FD-Score:4|P-value:3.23E-5||SGD DESC:Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine Gene:ARG7(YMR062C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:BIO5(YNR056C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CEX1(YOR112W)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:COA6(YMR244C-A_p)|FD-Score:4.74|P-value:1.07E-6||SGD DESC:Protein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress Gene:CPS1(YJL172W)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions Gene:DAL2(YIR029W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DSE1(YER124C)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Daughter cell-specific protein, may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ECM12(YHR021W-A_p)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity Gene:ERG4(YGL012W)|FD-Score:3.99|P-value:3.36E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FLO11(YIR019C)|FD-Score:-6.22|P-value:2.49E-10||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:FMP16(YDR070C_p)|FD-Score:4.5|P-value:3.48E-6||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:FRD1(YEL047C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:GCV3(YAL044C)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GGA1(YDR358W)|FD-Score:-3.29|P-value:5.01E-4||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GPB1(YOR371C)|FD-Score:-3.76|P-value:8.55E-5||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication Gene:HMT1(YBR034C)|FD-Score:3.73|P-value:9.54E-5||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HOG1(YLR113W)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:HXK1(YFR053C)|FD-Score:7.37|P-value:8.71E-14||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:MEP2(YNL142W)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MHF2(YDL160C-A)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 Gene:MOS2(YGR235C)|FD-Score:3.95|P-value:3.95E-5||SGD DESC:Mitochondrial inner membrane protein; non-essential component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:NBP2(YDR162C)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:OTU2(YHL013C)|FD-Score:4.46|P-value:4.16E-6||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PIR1(YKL164C)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication Gene:PRK1(YIL095W)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication Gene:PRY3(YJL078C)|FD-Score:-3.16|P-value:7.81E-4||SGD DESC:Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p Gene:PSK2(YOL045W)|FD-Score:-4.53|P-value:2.99E-6||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:PTK2(YJR059W)|FD-Score:-3.31|P-value:4.66E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RAX2(YLR084C)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:RGD2(YFL047W)|FD-Score:3.83|P-value:6.39E-5||SGD DESC:GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RIM4(YHL024W)|FD-Score:5.04|P-value:2.34E-7||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RMD6(YEL072W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Protein required for sporulation Gene:RNH203(YLR154C)|FD-Score:3.83|P-value:6.31E-5||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome Gene:RPL16B(YNL069C)|FD-Score:4.28|P-value:9.54E-6||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:SHM1(YBR263W)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine Gene:SNF6(YHL025W)|FD-Score:-4.96|P-value:3.60E-7||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SUM1(YDR310C)|FD-Score:3.77|P-value:8.26E-5||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SWM1(YDR260C)|FD-Score:3.97|P-value:3.65E-5||SGD DESC:Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation Gene:TRP3(YKL211C)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:USA1(YML029W)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p Gene:VAC7(YNL054W)|FD-Score:4.88|P-value:5.34E-7||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:YBR113W(YBR113W_d)|FD-Score:-3.21|P-value:6.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 Gene:YCL074W(YCL074W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YCR049C(YCR049C_d)|FD-Score:5.1|P-value:1.73E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR445C(YDR445C_d)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER186C(YER186C_p)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative protein of unknown function Gene:YFR018C(YFR018C_p)|FD-Score:4.28|P-value:9.40E-6||SGD DESC:Putative protein of unknown function Gene:YGL165C(YGL165C_d)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W Gene:YIP5(YGL161C)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YLR287C(YLR287C_p)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YNL122C(YNL122C_p)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YNL198C(YNL198C_d)|FD-Score:-5.24|P-value:8.20E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL295W(YNL295W_p)|FD-Score:-3.12|P-value:9.05E-4||SGD DESC:Putative protein of unknown function Gene:YOL019W(YOL019W_p)|FD-Score:4.01|P-value:2.98E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication Gene:YOR277C(YOR277C_d)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YOR292C(YOR292C_p)|FD-Score:-3.24|P-value:6.05E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene Gene:ALB1(YJL122W)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p Gene:ARG5,6(YER069W)|FD-Score:4|P-value:3.23E-5||SGD DESC:Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine Gene:ARG7(YMR062C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:BIO5(YNR056C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CEX1(YOR112W)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:COA6(YMR244C-A_p)|FD-Score:4.74|P-value:1.07E-6||SGD DESC:Protein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress Gene:CPS1(YJL172W)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions Gene:DAL2(YIR029W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DSE1(YER124C)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Daughter cell-specific protein, may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ECM12(YHR021W-A_p)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity Gene:ERG4(YGL012W)|FD-Score:3.99|P-value:3.36E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FLO11(YIR019C)|FD-Score:-6.22|P-value:2.49E-10||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:FMP16(YDR070C_p)|FD-Score:4.5|P-value:3.48E-6||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:FRD1(YEL047C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:GCV3(YAL044C)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GGA1(YDR358W)|FD-Score:-3.29|P-value:5.01E-4||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GPB1(YOR371C)|FD-Score:-3.76|P-value:8.55E-5||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication Gene:HMT1(YBR034C)|FD-Score:3.73|P-value:9.54E-5||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HOG1(YLR113W)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:HXK1(YFR053C)|FD-Score:7.37|P-value:8.71E-14||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:MEP2(YNL142W)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MHF2(YDL160C-A)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 Gene:MOS2(YGR235C)|FD-Score:3.95|P-value:3.95E-5||SGD DESC:Mitochondrial inner membrane protein; non-essential component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:NBP2(YDR162C)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:OTU2(YHL013C)|FD-Score:4.46|P-value:4.16E-6||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PIR1(YKL164C)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication Gene:PRK1(YIL095W)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication Gene:PRY3(YJL078C)|FD-Score:-3.16|P-value:7.81E-4||SGD DESC:Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p Gene:PSK2(YOL045W)|FD-Score:-4.53|P-value:2.99E-6||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:PTK2(YJR059W)|FD-Score:-3.31|P-value:4.66E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RAX2(YLR084C)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:RGD2(YFL047W)|FD-Score:3.83|P-value:6.39E-5||SGD DESC:GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RIM4(YHL024W)|FD-Score:5.04|P-value:2.34E-7||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RMD6(YEL072W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Protein required for sporulation Gene:RNH203(YLR154C)|FD-Score:3.83|P-value:6.31E-5||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome Gene:RPL16B(YNL069C)|FD-Score:4.28|P-value:9.54E-6||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:SHM1(YBR263W)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine Gene:SNF6(YHL025W)|FD-Score:-4.96|P-value:3.60E-7||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SUM1(YDR310C)|FD-Score:3.77|P-value:8.26E-5||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SWM1(YDR260C)|FD-Score:3.97|P-value:3.65E-5||SGD DESC:Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation Gene:TRP3(YKL211C)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:USA1(YML029W)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p Gene:VAC7(YNL054W)|FD-Score:4.88|P-value:5.34E-7||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:YBR113W(YBR113W_d)|FD-Score:-3.21|P-value:6.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 Gene:YCL074W(YCL074W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YCR049C(YCR049C_d)|FD-Score:5.1|P-value:1.73E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR445C(YDR445C_d)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER186C(YER186C_p)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative protein of unknown function Gene:YFR018C(YFR018C_p)|FD-Score:4.28|P-value:9.40E-6||SGD DESC:Putative protein of unknown function Gene:YGL165C(YGL165C_d)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W Gene:YIP5(YGL161C)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YLR287C(YLR287C_p)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YNL122C(YNL122C_p)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YNL198C(YNL198C_d)|FD-Score:-5.24|P-value:8.20E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL295W(YNL295W_p)|FD-Score:-3.12|P-value:9.05E-4||SGD DESC:Putative protein of unknown function Gene:YOL019W(YOL019W_p)|FD-Score:4.01|P-value:2.98E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication Gene:YOR277C(YOR277C_d)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YOR292C(YOR292C_p)|FD-Score:-3.24|P-value:6.05E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR291C16.706.59E-635.03GCD7Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YGR083C14.602.71E-485.03GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YOR260W11.505.74E-315.03GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YDR211W6.484.65E-110.93GCD6Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress
YPL238C_d5.541.47E-80.53YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YOR207C5.022.59E-70.19RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YDL152W_d4.827.00E-70.10YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YDL150W4.721.16E-60.28RPC53RNA polymerase III subunit C53
YBR247C4.444.43E-60.10ENP1Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus
YDL105W4.356.91E-60.01NSE4Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
YOR116C4.337.36E-60.12RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YLL008W4.211.26E-50.05DRS1Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles
YPL237W4.161.60E-50.27SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
YNL151C3.895.08E-50.17RPC31RNA polymerase III subunit C31
YNL002C3.729.89E-50.09RLP7Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YFR053C7.378.71E-14HXK1Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication
YDR445C_d5.191.03E-7YDR445C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YCR049C_d5.101.73E-7YCR049C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YHL024W5.042.34E-7RIM4Putative RNA-binding protein required for the expression of early and middle sporulation genes
YNL054W4.885.34E-7VAC7Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock
YMR244C-A_p4.741.07E-6COA6_pProtein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress
YDR070C_p4.503.48E-6FMP16_pProtein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
YHL013C4.464.16E-6OTU2Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress
YFR018C_p4.289.40E-6YFR018C_pPutative protein of unknown function
YNL069C4.289.54E-6RPL16BRibosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication
YKL164C4.062.48E-5PIR1O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication
YOL019W_p4.012.98E-5YOL019W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication
YER069W4.003.23E-5ARG5,6Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine
YGL012W3.993.36E-5ERG4C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
YDR260C3.973.65E-5SWM1Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation

GO enrichment analysis for SGTC_392
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2843.89E-110SGTC_2411st077133 39.9 μMTimTec (Natural product derivative library)14031420.0925926RNA pol III & RNase P/MRP
0.2221.28E-66SGTC_2612dictamnine 15.5 μMMicrosource (Natural product library)680850.0697674RNA pol III & RNase P/MRP
0.1653.42E-37SGTC_1872amphotericin b 148.0 nMMicrosource (Natural product library)166676790.0549451RNA pol III & RNase P/MRP
0.1606.71E-35SGTC_6113453-1652 23.7 μMChemDiv (Drug-like library)15605750.153846
0.1553.83E-33SGTC_2522biochanin a 4.9 μMMicrosource (Natural product library)52803730.102041RNA pol III & RNase P/MRP
0.1395.43E-27SGTC_870100-0052 35.5 μMChemDiv (Drug-like library)2001870.135135
0.1368.60E-26SGTC_2738aclarubicin 5.5 μMMiscellaneous4514150.07RNA pol III & RNase P/MRP
0.1315.38E-24SGTC_5394859-0462 2.5 μMChemDiv (Drug-like library)464994740.0526316RNA pol III & RNase P/MRP
0.1292.64E-23SGTC_1915st067105 30.6 μMTimTec (Natural product derivative library)697510.2RNA pol III & RNase P/MRP
0.1201.81E-20SGTC_274sirtinol 40.1 μMMiscellaneous68364420.105263
0.1158.05E-19SGTC_2544sphondin 68.5 μMMicrosource (Natural product library)1081040.133333RNA pol III & RNase P/MRP
0.1111.37E-17SGTC_1930st074634 34.3 μMTimTec (Natural product derivative library)6842530.113636RNA pol III & RNase P/MRP
0.0961.90E-13SGTC_23067482397 179.5 μMChembridge (Fragment library)8914480.0888889RPP1 & pyrimidine depletion
0.0921.72E-12SGTC_1768st044301 37.2 μMTimTec (Natural product derivative library)39313490.121212
0.0871.71E-11SGTC_5401187-0821 17.3 μMChemDiv (Drug-like library)8475200.0714286

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_20024023503131 μM0.3235294736863Chembridge (Fragment library)193.1744231.4303mitochondrial processes
SGTC_23369001819198.65 μM0.3235292989602Chembridge (Fragment library)209.629021.88902heme biosynthesis & mitochondrial translocase
SGTC_12220366-00741.18 μM0.314286286520ChemDiv (Drug-like library)245.230741.59204TSC3-RPN4
SGTC_12760871-006894.2 μM0.2647063414906ChemDiv (Drug-like library)261.319562.57104
SGTC_24625162487171.77 μM0.2619052259986Miscellaneous239.313962.79104
SGTC_12460422-0085105 μM0.258065264295ChemDiv (Drug-like library)161.157381.26512
SGTC_24345670830169.91 μM0.2558141549830Miscellaneous250.33992.67404
SGTC_21715739187198.08 μM0.2413792262761Chembridge (Fragment library)222.283520.90324copper-dependent oxidative stress
SGTC_9242046-004421.3 μM0.2368423623589ChemDiv (Drug-like library)312.40765.83202
SGTC_8470342-02745.54 μM0.23529418097ChemDiv (Drug-like library)218.165641.28204