dithiazanine

3-ethyl-2-[5-(3-ethyl-1,3-benzothiazol-3-ium-2-yl)penta-2,4-dienylidene]-1,3-benzothiazole iodide

An antihelmenthic.

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PubChem MeSH terms: Antinematodal Agents;Fluorescent Dyes



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_396
Screen concentration 1.2 μM
Source Miscellaneous
PubChem CID 10578
SMILES CCN1C2=CC=CC=C2SC1=CC=CC=CC3=[N+](C4=CC=CC=C4S3)CC.[I-]
Standardized SMILES CCN1C(=CC=CC=Cc2sc3ccccc3[n+]2CC)Sc4ccccc14
Molecular weight 518.4766
ALogP 3.93
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) -1.37


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 10578
Download HIP data (tab-delimited text)  (excel)
Gene:CSE4(YKL049C)|FD-Score:3.11|P-value:9.23E-4|Clearance:0.09||SGD DESC:Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions Gene:HEM12(YDR047W)|FD-Score:3.15|P-value:8.23E-4|Clearance:0.03||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:MCM3(YEL032W)|FD-Score:3.38|P-value:3.59E-4|Clearance:0.24||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:NOP8(YOL144W)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.03||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:RIM2(YBR192W)|FD-Score:4.21|P-value:1.28E-5|Clearance:0.29||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:SED5(YLR026C)|FD-Score:-3.1|P-value:9.76E-4|Clearance:0||SGD DESC:cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins Gene:STS1(YIR011C)|FD-Score:3.92|P-value:4.38E-5|Clearance:0.36||SGD DESC:Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation Gene:TOM40(YMR203W)|FD-Score:3.53|P-value:2.10E-4|Clearance:0.14||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YPL251W(YPL251W_d)|FD-Score:-4.12|P-value:1.91E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C Gene:YTH1(YPR107C)|FD-Score:7.57|P-value:1.88E-14|Clearance:3.36||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:CSE4(YKL049C)|FD-Score:3.11|P-value:9.23E-4|Clearance:0.09||SGD DESC:Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions Gene:HEM12(YDR047W)|FD-Score:3.15|P-value:8.23E-4|Clearance:0.03||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:MCM3(YEL032W)|FD-Score:3.38|P-value:3.59E-4|Clearance:0.24||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:NOP8(YOL144W)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.03||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:RIM2(YBR192W)|FD-Score:4.21|P-value:1.28E-5|Clearance:0.29||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:SED5(YLR026C)|FD-Score:-3.1|P-value:9.76E-4|Clearance:0||SGD DESC:cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins Gene:STS1(YIR011C)|FD-Score:3.92|P-value:4.38E-5|Clearance:0.36||SGD DESC:Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation Gene:TOM40(YMR203W)|FD-Score:3.53|P-value:2.10E-4|Clearance:0.14||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YPL251W(YPL251W_d)|FD-Score:-4.12|P-value:1.91E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C Gene:YTH1(YPR107C)|FD-Score:7.57|P-value:1.88E-14|Clearance:3.36||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 10578
Download HOP data (tab-delimited text)  (excel)
Gene:ARO7(YPR060C)|FD-Score:-3.35|P-value:4.04E-4||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:BNA5(YLR231C)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Kynureninase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:CSH1(YBR161W)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:CTL1(YMR180C)|FD-Score:-4.27|P-value:9.57E-6||SGD DESC:RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm Gene:DUR3(YHL016C)|FD-Score:-4.85|P-value:6.20E-7||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:ECM23(YPL021W)|FD-Score:4.58|P-value:2.28E-6||SGD DESC:Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p Gene:FCY21(YER060W)|FD-Score:-3.25|P-value:5.77E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FMP30(YPL103C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D Gene:FUB1(YCR076C)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene Gene:FUN14(YAL008W)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:Mitochondrial protein of unknown function Gene:GIS3(YLR094C)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Protein of unknown function Gene:HNM1(YGL077C)|FD-Score:5.64|P-value:8.32E-9||SGD DESC:Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol Gene:IRC14(YOR135C_d)|FD-Score:4.65|P-value:1.63E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:KEL1(YHR158C)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LEU4(YNL104C)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication Gene:MDM34(YGL219C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MOD5(YOR274W)|FD-Score:-4.04|P-value:2.69E-5||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MUS81(YDR386W)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:NEW1(YPL226W)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:OPI7(YDR360W_d)|FD-Score:-3.14|P-value:8.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:PKR1(YMR123W)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PUF4(YGL014W)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:REC104(YHR157W)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:REG1(YDR028C)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:REX2(YLR059C)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:SIZ1(YDR409W)|FD-Score:5.9|P-value:1.86E-9||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SRO9(YCL037C)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:STL1(YDR536W)|FD-Score:-4.3|P-value:8.44E-6||SGD DESC:Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock Gene:TGS1(YPL157W)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TPK1(YJL164C)|FD-Score:-3.12|P-value:9.16E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication Gene:VMA7(YGR020C)|FD-Score:-3.87|P-value:5.36E-5||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS51(YKR020W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:WHI2(YOR043W)|FD-Score:3.77|P-value:8.17E-5||SGD DESC:Protein required, with binding partner Psr1p, for full activation of the general stress response, possibly through Msn2p dephosphorylation; regulates growth during the diauxic shift; negative regulator of G1 cyclin expression Gene:YAL042C-A(YAL042C-A_d)|FD-Score:3.76|P-value:8.39E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YBR053C(YBR053C_p)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Putative protein of unknown function; induced by cell wall perturbation Gene:YBR197C(YBR197C_p)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication Gene:YCP4(YCR004C)|FD-Score:-3.09|P-value:9.86E-4||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YDL094C(YDL094C_d)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential Gene:YML096W(YML096W_p)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YMR316C-B(YMR316C-B_d)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL014W(YOL014W_p)|FD-Score:-3.1|P-value:9.61E-4||SGD DESC:Putative protein of unknown function Gene:YOR343C(YOR343C_d)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR385W(YOR385W_p)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YOX1(YML027W)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication Gene:YPR114W(YPR114W_p)|FD-Score:3.72|P-value:9.93E-5||SGD DESC:Putative protein of unknown function Gene:ARO7(YPR060C)|FD-Score:-3.35|P-value:4.04E-4||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:BNA5(YLR231C)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Kynureninase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:CSH1(YBR161W)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:CTL1(YMR180C)|FD-Score:-4.27|P-value:9.57E-6||SGD DESC:RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm Gene:DUR3(YHL016C)|FD-Score:-4.85|P-value:6.20E-7||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:ECM23(YPL021W)|FD-Score:4.58|P-value:2.28E-6||SGD DESC:Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p Gene:FCY21(YER060W)|FD-Score:-3.25|P-value:5.77E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FMP30(YPL103C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D Gene:FUB1(YCR076C)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene Gene:FUN14(YAL008W)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:Mitochondrial protein of unknown function Gene:GIS3(YLR094C)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Protein of unknown function Gene:HNM1(YGL077C)|FD-Score:5.64|P-value:8.32E-9||SGD DESC:Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol Gene:IRC14(YOR135C_d)|FD-Score:4.65|P-value:1.63E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:KEL1(YHR158C)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LEU4(YNL104C)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication Gene:MDM34(YGL219C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MOD5(YOR274W)|FD-Score:-4.04|P-value:2.69E-5||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MUS81(YDR386W)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:NEW1(YPL226W)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:OPI7(YDR360W_d)|FD-Score:-3.14|P-value:8.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:PKR1(YMR123W)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PUF4(YGL014W)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:REC104(YHR157W)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:REG1(YDR028C)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:REX2(YLR059C)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:SIZ1(YDR409W)|FD-Score:5.9|P-value:1.86E-9||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SRO9(YCL037C)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:STL1(YDR536W)|FD-Score:-4.3|P-value:8.44E-6||SGD DESC:Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock Gene:TGS1(YPL157W)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TPK1(YJL164C)|FD-Score:-3.12|P-value:9.16E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication Gene:VMA7(YGR020C)|FD-Score:-3.87|P-value:5.36E-5||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS51(YKR020W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:WHI2(YOR043W)|FD-Score:3.77|P-value:8.17E-5||SGD DESC:Protein required, with binding partner Psr1p, for full activation of the general stress response, possibly through Msn2p dephosphorylation; regulates growth during the diauxic shift; negative regulator of G1 cyclin expression Gene:YAL042C-A(YAL042C-A_d)|FD-Score:3.76|P-value:8.39E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YBR053C(YBR053C_p)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Putative protein of unknown function; induced by cell wall perturbation Gene:YBR197C(YBR197C_p)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication Gene:YCP4(YCR004C)|FD-Score:-3.09|P-value:9.86E-4||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YDL094C(YDL094C_d)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential Gene:YML096W(YML096W_p)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YMR316C-B(YMR316C-B_d)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL014W(YOL014W_p)|FD-Score:-3.1|P-value:9.61E-4||SGD DESC:Putative protein of unknown function Gene:YOR343C(YOR343C_d)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR385W(YOR385W_p)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YOX1(YML027W)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication Gene:YPR114W(YPR114W_p)|FD-Score:3.72|P-value:9.93E-5||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR107C7.571.88E-143.36YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YBR192W4.211.28E-50.29RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YIR011C3.924.38E-50.36STS1Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation
YOL144W3.561.85E-40.03NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YMR203W3.532.10E-40.14TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YEL032W3.383.59E-40.23MCM3Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
YDR047W3.158.23E-40.03HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YKL049C3.119.23E-40.09CSE4Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions
YBL030C3.020.001250.01PET9Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication
YMR208W3.010.001300.13ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YIL126W2.880.002000.02STH1ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
YDR362C2.860.002090.01TFC6One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
YLR033W2.860.002140.07RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YOR117W2.790.002650.02RPT5One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
YMR076C2.770.002830.04PDS5Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR409W5.901.86E-9SIZ1SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring
YGL077C5.648.32E-9HNM1Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol
YOR135C_d4.651.63E-6IRC14_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci
YPL021W4.582.28E-6ECM23Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p
YCR076C4.201.35E-5FUB1Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene
YLR094C4.013.01E-5GIS3Protein of unknown function
YDL094C_d3.983.46E-5YDL094C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential
YOR043W3.778.17E-5WHI2Protein required, with binding partner Psr1p, for full activation of the general stress response, possibly through Msn2p dephosphorylation; regulates growth during the diauxic shift; negative regulator of G1 cyclin expression
YAL042C-A_d3.768.39E-5YAL042C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene
YPR114W_p3.729.93E-5YPR114W_pPutative protein of unknown function
YKR020W3.701.09E-4VPS51Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p
YOR385W_p3.541.97E-4YOR385W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene
YPL103C3.492.41E-4FMP30Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D
YPL157W3.462.73E-4TGS1Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
YBR197C_p3.452.82E-4YBR197C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication

GO enrichment analysis for SGTC_396
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1738.29E-41SGTC_30641327-0027 10.0 μMChemDiv (Drug-like library)160004550.0697674
0.1315.14E-24SGTC_30601327-0027 12.5 μMChemDiv (Drug-like library)160004550.0697674
0.1301.21E-23SGTC_1113artemisinin 1.2 μMTimTec (Natural product library)22400.0235294heme biosynthesis & mitochondrial translocase
0.1249.35E-22SGTC_24295902844 64.4 μMMiscellaneous19277900.121622
0.1211.24E-20SGTC_30591327-0034 3.0 μMChemDiv (Drug-like library)159985800.0337079
0.1111.21E-17SGTC_30631327-0034 4.0 μMChemDiv (Drug-like library)159985800.0337079
0.1081.16E-16SGTC_5860957-0265 8.2 μMChemDiv (Drug-like library)67523090.0816327cell wall
0.1064.00E-16SGTC_1968st069590 43.6 μMTimTec (Natural product derivative library)63840660.0875
0.1016.33E-15SGTC_1831st055328 12.0 μMTimTec (Natural product derivative library)11030680.0921053heme biosynthesis & mitochondrial translocase
0.0992.83E-14SGTC_6740498-0004 451.0 μMChemDiv (Drug-like library)42314730.046875heme requiring
0.0993.27E-14SGTC_15526-methylflavone 10.6 μMTimTec (Pure natural product library)6890130.08superoxide
0.0983.78E-14SGTC_7301436-0052 41.2 μMChemDiv (Drug-like library)7687640.0617284mitochondrial processes
0.0985.78E-14SGTC_208k072-0098 12.4 μMChemDiv (Drug-like library)59489010.0657895heme biosynthesis & mitochondrial translocase
0.0945.48E-13SGTC_14224031-0024 15.5 μMChemDiv (Drug-like library)7824270.0740741iron homeostasis
0.0938.50E-13SGTC_21395578692 56.5 μMChembridge (Fragment library)6509910.0519481superoxide

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_13101188-0297994 nM0.651588352ChemDiv (Drug-like library)393.5887.47503azole & statin
SGTC_3832324-03957.37 μM0.3913043146421ChemDiv (Drug-like library)361.5237.25302azole & statin
SGTC_6564090-1979187 μM0.31645622330961ChemDiv (Drug-like library)393.607926.6102
SGTC_264promethazine12.5 mM0.2786896014Miscellaneous320.880044.77213DNA intercalators
SGTC_6001495-041841.6 μM0.2615385377768ChemDiv (Drug-like library)267.345524.36603RPP1 & pyrimidine depletion
SGTC_13812324-006662.1 μM0.25316510885928ChemDiv (Drug-like library)402.293766.78503
SGTC_2728chlorpromazine9.18 μM0.2428572726Miscellaneous318.864164.73903
SGTC_5471120-0019610 nM0.2325583676939ChemDiv (Drug-like library)455.013667.96501azole & statin
SGTC_9021120-00022.07 μM0.2325583280475ChemDiv (Drug-like library)455.013667.96501azole & statin
SGTC_11720370-004385 μM0.231884376585ChemDiv (Drug-like library)355.520145.28504