bronopol

2-bromo-2-nitropropane-1,3-diol

Metabolized to formaldehyde. Reacts with secondary amines and cross-links them.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_398
Screen concentration 33.3 μM
Source Miscellaneous
PubChem CID 2450
SMILES C(C(CO)([N+](=O)[O-])Br)O
Standardized SMILES OCC(Br)(CO)[N+](=O)[O-]
Molecular weight 199.988
ALogP 0.06
H-bond donor count 2
H-bond acceptor count 4
Response signature RNA processing & uracil transport

Pool Growth Kinetics
% growth inhibition (Het. pool) 27.7
% growth inhibition (Hom. pool) 17.78


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2450
Download HIP data (tab-delimited text)  (excel)
Gene:ARP4(YJL081C)|FD-Score:3.76|P-value:8.48E-5|Clearance:0.03||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:CBK1(YNL161W)|FD-Score:4.3|P-value:8.64E-6|Clearance:0.09||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:CCT4(YDL143W)|FD-Score:-4.37|P-value:6.13E-6|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:ERG12(YMR208W)|FD-Score:4.12|P-value:1.86E-5|Clearance:0.31||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GLC7(YER133W)|FD-Score:4.21|P-value:1.29E-5|Clearance:0.08||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI8(YDR331W)|FD-Score:3.11|P-value:9.29E-4|Clearance:0.15||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:KEI1(YDR367W)|FD-Score:3.55|P-value:1.95E-4|Clearance:0.13||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:LTO1(YNL260C_p)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.04||SGD DESC:Essential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species Gene:MCD4(YKL165C)|FD-Score:-5.38|P-value:3.68E-8|Clearance:0||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MCM7(YBR202W)|FD-Score:3.42|P-value:3.12E-4|Clearance:0.18||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MTW1(YAL034W-A)|FD-Score:3.8|P-value:7.16E-5|Clearance:0.04||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly Gene:NOP58(YOR310C)|FD-Score:-5.06|P-value:2.13E-7|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NOP8(YOL144W)|FD-Score:6.41|P-value:7.49E-11|Clearance:1.04||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PMI40(YER003C)|FD-Score:4.62|P-value:1.88E-6|Clearance:0.08||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PUP3(YER094C)|FD-Score:5.37|P-value:4.01E-8|Clearance:0.69||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RIM2(YBR192W)|FD-Score:3.21|P-value:6.71E-4|Clearance:0.08||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPL25(YOL127W)|FD-Score:-5.75|P-value:4.56E-9|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RRS1(YOR294W)|FD-Score:3.24|P-value:5.92E-4|Clearance:0.04||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:RSC58(YLR033W)|FD-Score:4.4|P-value:5.41E-6|Clearance:0.1||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:4.68|P-value:1.46E-6|Clearance:0.05||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SCC2(YDR180W)|FD-Score:3.73|P-value:9.50E-5|Clearance:0.02||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin Gene:SEC61(YLR378C)|FD-Score:-3.51|P-value:2.22E-4|Clearance:0||SGD DESC:Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER Gene:SMC3(YJL074C)|FD-Score:4.55|P-value:2.69E-6|Clearance:0.15||SGD DESC:Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member Gene:SPT6(YGR116W)|FD-Score:-3.52|P-value:2.14E-4|Clearance:0||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:SRP1(YNL189W)|FD-Score:3.73|P-value:9.40E-5|Clearance:0||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:TAF12(YDR145W)|FD-Score:3.81|P-value:6.90E-5|Clearance:0.01||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TFC6(YDR362C)|FD-Score:3.13|P-value:8.80E-4|Clearance:0.02||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TOA2(YKL058W)|FD-Score:3.66|P-value:1.25E-4|Clearance:0.01||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:YGR114C(YGR114C_d)|FD-Score:-8.37|P-value:2.80E-17|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YJL086C(YJL086C_d)|FD-Score:3.71|P-value:1.04E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR076C(YLR076C_d)|FD-Score:-3.37|P-value:3.81E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YTH1(YPR107C)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.07||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ARP4(YJL081C)|FD-Score:3.76|P-value:8.48E-5|Clearance:0.03||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:CBK1(YNL161W)|FD-Score:4.3|P-value:8.64E-6|Clearance:0.09||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:CCT4(YDL143W)|FD-Score:-4.37|P-value:6.13E-6|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:ERG12(YMR208W)|FD-Score:4.12|P-value:1.86E-5|Clearance:0.31||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GLC7(YER133W)|FD-Score:4.21|P-value:1.29E-5|Clearance:0.08||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI8(YDR331W)|FD-Score:3.11|P-value:9.29E-4|Clearance:0.15||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:KEI1(YDR367W)|FD-Score:3.55|P-value:1.95E-4|Clearance:0.13||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:LTO1(YNL260C_p)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.04||SGD DESC:Essential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species Gene:MCD4(YKL165C)|FD-Score:-5.38|P-value:3.68E-8|Clearance:0||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MCM7(YBR202W)|FD-Score:3.42|P-value:3.12E-4|Clearance:0.18||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MTW1(YAL034W-A)|FD-Score:3.8|P-value:7.16E-5|Clearance:0.04||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly Gene:NOP58(YOR310C)|FD-Score:-5.06|P-value:2.13E-7|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NOP8(YOL144W)|FD-Score:6.41|P-value:7.49E-11|Clearance:1.04||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PMI40(YER003C)|FD-Score:4.62|P-value:1.88E-6|Clearance:0.08||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PUP3(YER094C)|FD-Score:5.37|P-value:4.01E-8|Clearance:0.69||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RIM2(YBR192W)|FD-Score:3.21|P-value:6.71E-4|Clearance:0.08||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPL25(YOL127W)|FD-Score:-5.75|P-value:4.56E-9|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RRS1(YOR294W)|FD-Score:3.24|P-value:5.92E-4|Clearance:0.04||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:RSC58(YLR033W)|FD-Score:4.4|P-value:5.41E-6|Clearance:0.1||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:4.68|P-value:1.46E-6|Clearance:0.05||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SCC2(YDR180W)|FD-Score:3.73|P-value:9.50E-5|Clearance:0.02||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin Gene:SEC61(YLR378C)|FD-Score:-3.51|P-value:2.22E-4|Clearance:0||SGD DESC:Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER Gene:SMC3(YJL074C)|FD-Score:4.55|P-value:2.69E-6|Clearance:0.15||SGD DESC:Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member Gene:SPT6(YGR116W)|FD-Score:-3.52|P-value:2.14E-4|Clearance:0||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:SRP1(YNL189W)|FD-Score:3.73|P-value:9.40E-5|Clearance:0||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:TAF12(YDR145W)|FD-Score:3.81|P-value:6.90E-5|Clearance:0.01||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TFC6(YDR362C)|FD-Score:3.13|P-value:8.80E-4|Clearance:0.02||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TOA2(YKL058W)|FD-Score:3.66|P-value:1.25E-4|Clearance:0.01||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:YGR114C(YGR114C_d)|FD-Score:-8.37|P-value:2.80E-17|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YJL086C(YJL086C_d)|FD-Score:3.71|P-value:1.04E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR076C(YLR076C_d)|FD-Score:-3.37|P-value:3.81E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YTH1(YPR107C)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.07||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2450
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:APL2(YKL135C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:ARC18(YLR370C)|FD-Score:4|P-value:3.10E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARG82(YDR173C)|FD-Score:4.31|P-value:8.34E-6||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ARO1(YDR127W)|FD-Score:-4.04|P-value:2.68E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:BRP1(YGL007W_d)|FD-Score:7.92|P-value:1.23E-15||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BTS1(YPL069C)|FD-Score:-4.71|P-value:1.23E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD28(YLR062C_d)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAF130(YGR134W)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation Gene:CCS1(YMR038C)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CDC73(YLR418C)|FD-Score:10.4|P-value:9.71E-26||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CNL1(YDR357C_p)|FD-Score:4.72|P-value:1.18E-6||SGD DESC:Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:COA2(YPL189C-A)|FD-Score:-4.02|P-value:2.88E-5||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:COG5(YNL051W)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:-3.17|P-value:7.72E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-4|P-value:3.11E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTK1(YKL139W)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:EGD2(YHR193C)|FD-Score:6.9|P-value:2.58E-12||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:ERG4(YGL012W)|FD-Score:6.16|P-value:3.70E-10||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FET4(YMR319C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FRE1(YLR214W)|FD-Score:-6.93|P-value:2.17E-12||SGD DESC:Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels Gene:FUR4(YBR021W)|FD-Score:6.87|P-value:3.14E-12||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GAS4(YOL132W)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas2p in spore wall assembly; has similarity to Gas1p; localizes to the cell wall Gene:GCN4(YEL009C)|FD-Score:-4.31|P-value:8.19E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GDE1(YPL110C)|FD-Score:-3.87|P-value:5.46E-5||SGD DESC:Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes Gene:GIN4(YDR507C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:GLY1(YEL046C)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GSH1(YJL101C)|FD-Score:7.53|P-value:2.49E-14||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:GSH2(YOL049W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock Gene:GTR1(YML121W)|FD-Score:5.28|P-value:6.55E-8||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:GTR2(YGR163W)|FD-Score:4.86|P-value:5.81E-7||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GYP1(YOR070C)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HEH2(YDR458C)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HOS1(YPR068C)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:HRB1(YNL004W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication Gene:HXK2(YGL253W)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:IES5(YER092W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:IRS4(YKR019C)|FD-Score:-4.66|P-value:1.60E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KIP3(YGL216W)|FD-Score:5.04|P-value:2.36E-7||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning Gene:LSM1(YJL124C)|FD-Score:4.31|P-value:8.32E-6||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LSM7(YNL147W)|FD-Score:-3.28|P-value:5.10E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:LTE1(YAL024C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MCR1(YKL150W)|FD-Score:-3.91|P-value:4.53E-5||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MEF2(YJL102W)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MIH1(YMR036C)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 Gene:MRPL22(YNL177C)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL23(YOR150W)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSN2(YMR037C)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:MSW1(YDR268W)|FD-Score:-3.85|P-value:5.90E-5||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NHX1(YDR456W)|FD-Score:6.66|P-value:1.38E-11||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NOP6(YDL213C)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:PAL1(YDR348C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PBP1(YGR178C)|FD-Score:5.31|P-value:5.39E-8||SGD DESC:Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:PHO84(YML123C)|FD-Score:-3.18|P-value:7.26E-4||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PIB2(YGL023C)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PUF3(YLL013C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:QRI5(YLR204W)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RPL34B(YIL052C)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-7.91|P-value:1.28E-15||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS18A(YDR450W)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RPS18B(YML026C)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RSM23(YGL129C)|FD-Score:5.41|P-value:3.08E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p Gene:RTF1(YGL244W)|FD-Score:4.84|P-value:6.48E-7||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:RTT103(YDR289C)|FD-Score:5.01|P-value:2.66E-7||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:RXT2(YBR095C)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SAC7(YDR389W)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SEC22(YLR268W)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SPT4(YGR063C)|FD-Score:-3.33|P-value:4.41E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:STP1(YDR463W)|FD-Score:-3.25|P-value:5.73E-4||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SWT1(YOR166C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain Gene:TPO4(YOR273C)|FD-Score:4.47|P-value:3.89E-6||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRP2(YER090W)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-4.99|P-value:2.99E-7||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP5(YGL026C)|FD-Score:-3.8|P-value:7.29E-5||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:9.1|P-value:4.60E-20||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UTR1(YJR049C)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VPS45(YGL095C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS72(YDR485C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBL071C-B(YBL071C-B_p)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR174C(YBR174C_d)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YBR238C(YBR238C)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YDL118W(YDL118W_p)|FD-Score:-3.88|P-value:5.17E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDL119C(YDL119C_p)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDL124W(YDL124W)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:NADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress Gene:YDR290W(YDR290W_d)|FD-Score:8.27|P-value:6.78E-17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YDR455C(YDR455C_d)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YEL045C(YEL045C_d)|FD-Score:-5.06|P-value:2.14E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YER066C-A(YER066C-A_d)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, partially overlaps uncharacterized ORF YER067W Gene:YGL242C(YGL242C_p)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YHP1(YDR451C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YKL044W(YKL044W_p)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL075C(YKL075C_p)|FD-Score:6.49|P-value:4.28E-11||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YKR073C(YKR073C_d)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML122C(YML122C_d)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL050C(YOL050C_d)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOL099C(YOL099C_d)|FD-Score:4.71|P-value:1.22E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YPR027C(YPR027C_p)|FD-Score:-3.17|P-value:7.69E-4||SGD DESC:Putative protein of unknown function Gene:YPT11(YNL304W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells Gene:ACB1(YGR037C)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:APL2(YKL135C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:ARC18(YLR370C)|FD-Score:4|P-value:3.10E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARG82(YDR173C)|FD-Score:4.31|P-value:8.34E-6||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ARO1(YDR127W)|FD-Score:-4.04|P-value:2.68E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:BRP1(YGL007W_d)|FD-Score:7.92|P-value:1.23E-15||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BTS1(YPL069C)|FD-Score:-4.71|P-value:1.23E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD28(YLR062C_d)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAF130(YGR134W)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation Gene:CCS1(YMR038C)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CDC73(YLR418C)|FD-Score:10.4|P-value:9.71E-26||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CNL1(YDR357C_p)|FD-Score:4.72|P-value:1.18E-6||SGD DESC:Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:COA2(YPL189C-A)|FD-Score:-4.02|P-value:2.88E-5||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:COG5(YNL051W)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:-3.17|P-value:7.72E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-4|P-value:3.11E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTK1(YKL139W)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:EGD2(YHR193C)|FD-Score:6.9|P-value:2.58E-12||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:ERG4(YGL012W)|FD-Score:6.16|P-value:3.70E-10||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FET4(YMR319C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FRE1(YLR214W)|FD-Score:-6.93|P-value:2.17E-12||SGD DESC:Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels Gene:FUR4(YBR021W)|FD-Score:6.87|P-value:3.14E-12||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GAS4(YOL132W)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas2p in spore wall assembly; has similarity to Gas1p; localizes to the cell wall Gene:GCN4(YEL009C)|FD-Score:-4.31|P-value:8.19E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GDE1(YPL110C)|FD-Score:-3.87|P-value:5.46E-5||SGD DESC:Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes Gene:GIN4(YDR507C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:GLY1(YEL046C)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GSH1(YJL101C)|FD-Score:7.53|P-value:2.49E-14||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:GSH2(YOL049W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock Gene:GTR1(YML121W)|FD-Score:5.28|P-value:6.55E-8||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:GTR2(YGR163W)|FD-Score:4.86|P-value:5.81E-7||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GYP1(YOR070C)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HEH2(YDR458C)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HOS1(YPR068C)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:HRB1(YNL004W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication Gene:HXK2(YGL253W)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:IES5(YER092W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:IRS4(YKR019C)|FD-Score:-4.66|P-value:1.60E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KIP3(YGL216W)|FD-Score:5.04|P-value:2.36E-7||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning Gene:LSM1(YJL124C)|FD-Score:4.31|P-value:8.32E-6||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LSM7(YNL147W)|FD-Score:-3.28|P-value:5.10E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:LTE1(YAL024C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MCR1(YKL150W)|FD-Score:-3.91|P-value:4.53E-5||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MEF2(YJL102W)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MIH1(YMR036C)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 Gene:MRPL22(YNL177C)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL23(YOR150W)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSN2(YMR037C)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:MSW1(YDR268W)|FD-Score:-3.85|P-value:5.90E-5||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NHX1(YDR456W)|FD-Score:6.66|P-value:1.38E-11||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NOP6(YDL213C)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:PAL1(YDR348C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PBP1(YGR178C)|FD-Score:5.31|P-value:5.39E-8||SGD DESC:Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:PHO84(YML123C)|FD-Score:-3.18|P-value:7.26E-4||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PIB2(YGL023C)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PUF3(YLL013C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:QRI5(YLR204W)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RPL34B(YIL052C)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-7.91|P-value:1.28E-15||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS18A(YDR450W)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RPS18B(YML026C)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RSM23(YGL129C)|FD-Score:5.41|P-value:3.08E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p Gene:RTF1(YGL244W)|FD-Score:4.84|P-value:6.48E-7||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:RTT103(YDR289C)|FD-Score:5.01|P-value:2.66E-7||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:RXT2(YBR095C)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SAC7(YDR389W)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SEC22(YLR268W)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SPT4(YGR063C)|FD-Score:-3.33|P-value:4.41E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:STP1(YDR463W)|FD-Score:-3.25|P-value:5.73E-4||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SWT1(YOR166C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain Gene:TPO4(YOR273C)|FD-Score:4.47|P-value:3.89E-6||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRP2(YER090W)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-4.99|P-value:2.99E-7||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP5(YGL026C)|FD-Score:-3.8|P-value:7.29E-5||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:9.1|P-value:4.60E-20||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UTR1(YJR049C)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VPS45(YGL095C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS72(YDR485C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBL071C-B(YBL071C-B_p)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR174C(YBR174C_d)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YBR238C(YBR238C)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YDL118W(YDL118W_p)|FD-Score:-3.88|P-value:5.17E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDL119C(YDL119C_p)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDL124W(YDL124W)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:NADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress Gene:YDR290W(YDR290W_d)|FD-Score:8.27|P-value:6.78E-17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YDR455C(YDR455C_d)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YEL045C(YEL045C_d)|FD-Score:-5.06|P-value:2.14E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YER066C-A(YER066C-A_d)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, partially overlaps uncharacterized ORF YER067W Gene:YGL242C(YGL242C_p)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YHP1(YDR451C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YKL044W(YKL044W_p)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL075C(YKL075C_p)|FD-Score:6.49|P-value:4.28E-11||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YKR073C(YKR073C_d)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML122C(YML122C_d)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL050C(YOL050C_d)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOL099C(YOL099C_d)|FD-Score:4.71|P-value:1.22E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YPR027C(YPR027C_p)|FD-Score:-3.17|P-value:7.69E-4||SGD DESC:Putative protein of unknown function Gene:YPT11(YNL304W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOL144W6.417.49E-111.04NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YER094C5.374.01E-80.69PUP3Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
YCR052W4.681.46E-60.05RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YER003C4.621.88E-60.08PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YJL074C4.552.69E-60.15SMC3Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member
YLR033W4.405.41E-60.10RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YNL161W4.308.64E-60.09CBK1Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
YER133W4.211.29E-50.08GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YMR208W4.121.86E-50.31ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YDR145W3.816.90E-50.01TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YAL034W-A3.807.16E-50.04MTW1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly
YJL081C3.768.48E-50.03ARP4Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YNL189W3.739.40E-50.00SRP1Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation
YDR180W3.739.50E-50.02SCC2Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin
YJL086C_d3.711.04E-40.05YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR418C10.409.71E-26CDC73Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress
YBR058C-A9.104.60E-20TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YDR290W_d8.276.78E-17YDR290W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103
YGL007W_d7.921.23E-15BRP1_dDubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1
YJL101C7.532.49E-14GSH1Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
YHR193C6.902.58E-12EGD2Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes
YBR021W6.873.14E-12FUR4Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
YDR456W6.661.38E-11NHX1Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion
YKL075C_p6.494.28E-11YKL075C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin
YGL012W6.163.70E-10ERG4C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
YGL129C5.413.08E-8RSM23Mitochondrial ribosomal protein of the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p
YGR178C5.315.39E-8PBP1Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
YML121W5.286.55E-8GTR1Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
YGL216W5.042.36E-7KIP3Kinesin-related motor protein involved in mitotic spindle positioning
YDR289C5.012.66E-7RTT103Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition

GO enrichment analysis for SGTC_398
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1883.89E-48SGTC_21115486399 200.0 μMChembridge (Fragment library)6896740.0212766
0.1602.81E-35SGTC_20825219693 150.0 μMChembridge (Fragment library)28346490.0204082RNA processing & uracil transport
0.1503.70E-31SGTC_467ro 31-8220 72.8 μMICCB bioactive library50830.0307692calcium & mitochondrial duress
0.1481.75E-30SGTC_21105483698 200.0 μMChembridge (Fragment library)2848765NATSC3-RPN4
0.1476.47E-30SGTC_9231900-2434 73.3 μMChemDiv (Drug-like library)5910570NARNA processing & uracil transport
0.1461.64E-29SGTC_22256654152 15.4 μMChembridge (Fragment library)975820.0540541
0.1452.90E-29SGTC_5394859-0462 2.5 μMChemDiv (Drug-like library)464994740.0892857RNA pol III & RNase P/MRP
0.1432.22E-28SGTC_1745st081495 74.0 μMTimTec (Natural product derivative library)138424020.037037RNA processing & uracil transport
0.1411.14E-27SGTC_1220236-0016 402.4 μMChemDiv (Drug-like library)68088380.0447761RNA processing & uracil transport
0.1411.47E-27SGTC_1950st077106 7.2 μMTimTec (Natural product derivative library)138318640.0422535RNA processing & uracil transport
0.1372.92E-26SGTC_2551apiole 73.9 μMMicrosource (Natural product library)106590.0204082
0.1373.70E-26SGTC_23519026858 72.3 μMChembridge (Fragment library)14093000.0425532RNA processing & uracil transport
0.1345.49E-25SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0212766TSC3-RPN4
0.1347.44E-25SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.0208333
0.1338.80E-25SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.030303TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_890302-0250142.11 μM0.1794874203518ChemDiv (Drug-like library)255.227322.23616
SGTC_30601327-002712.5 μM0.17647116000455ChemDiv (Drug-like library)454.123145.74815
SGTC_30641327-002710 μM0.17647116000455ChemDiv (Drug-like library)454.123145.74815
SGTC_16erodoxin22.81 μM0.1621623967339ChemDiv (Drug-like library)277.0292.3505unfolded protein response
SGTC_9821491-051827.5 μM0.163091256ChemDiv (Drug-like library)416.265653.336211
SGTC_1631327-00367.55 μM0.15686315992207ChemDiv (Drug-like library)432.117625.56615
SGTC_26511327-00365 μM0.15686315992207ChemDiv (Drug-like library)432.117625.56615
SGTC_30591327-00343 μM0.15384615998580ChemDiv (Drug-like library)446.14426.02315
SGTC_30631327-00344 μM0.15384615998580ChemDiv (Drug-like library)446.14426.02315
SGTC_520329-0193875 nM0.1521743739292ChemDiv (Drug-like library)325.575642.80814TSC3-RPN4
SGTC_1463448-865053.13 μM0.152891488ChemDiv (Drug-like library)449.34153.82226NEO1-PIK1
SGTC_480382-001855 μM0.146341762596ChemDiv (Drug-like library)231.204163.04214
SGTC_1893970-07422.12 μM0.1463412891807ChemDiv (Drug-like library)297.06172.03104