Deletion Strain | FD score | P-value | Clearance | Gene | Gene Description |
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YDR353W | 8.70 | 1.62E-18 | 2.87 | TRR1 | Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication |
YCL043C | 5.83 | 2.75E-9 | 0.58 | PDI1 | Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication |
YBL084C | 5.25 | 7.69E-8 | 0.59 | CDC27 | Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition |
YLR359W | 4.66 | 1.58E-6 | 0.56 | ADE13 | Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency |
YDR240C | 4.10 | 2.03E-5 | 0.38 | SNU56 | Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex |
YGR245C | 3.73 | 9.71E-5 | 0.12 | SDA1 | Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis |
YBL014C | 3.61 | 1.56E-4 | 0.05 | RRN6 | Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p |
YDR473C | 3.55 | 1.90E-4 | 0.11 | PRP3 | Splicing factor, component of the U4/U6-U5 snRNP complex |
YOL077C | 3.44 | 2.91E-4 | 0.24 | BRX1 | Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif |
YNL124W | 3.20 | 6.79E-4 | 0.05 | NAF1 | RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p |
YDR427W | 3.15 | 8.03E-4 | 0.03 | RPN9 | Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress |
YDL163W_d | 3.13 | 8.76E-4 | 0.08 | YDL163W_d | Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase |
YHR036W | 3.05 | 0.00116 | 0.00 | BRL1 | Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export |
YOR174W | 3.05 | 0.00116 | 0.19 | MED4 | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation |
YKL173W | 2.86 | 0.00213 | 0.05 | SNU114 | GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 |