arphamenine a

(2R,5S)-5-amino-2-benzyl-8-(diaminomethylideneamino)-4-oxooctanoic acid

An arginine aminopeptidase inhibitor.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_403
Screen concentration 50.0 μM
Source Miscellaneous
PubChem CID 5487158
SMILES C1=CC=C(C=C1)CC(CC(=O)C(CCCN=C(N)N)N)C(=O)O
Standardized SMILES NC(CCCN=C(N)N)C(=O)CC(Cc1ccccc1)C(=O)O
Molecular weight 320.3868
ALogP -1.93
H-bond donor count 4
H-bond acceptor count 5
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 2.34
% growth inhibition (Hom. pool) -0.41


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5487158
Download HIP data (tab-delimited text)  (excel)
Gene:ARP7(YPR034W)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.13||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRL1(YHR036W)|FD-Score:3.45|P-value:2.78E-4|Clearance:0.16||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CAB2(YIL083C)|FD-Score:3.53|P-value:2.11E-4|Clearance:0.01||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:CCT3(YJL014W)|FD-Score:3.8|P-value:7.36E-5|Clearance:0.14||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDS1(YBR029C)|FD-Score:-4.56|P-value:2.53E-6|Clearance:0||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:GDI1(YER136W)|FD-Score:3.29|P-value:4.95E-4|Clearance:0.04||SGD DESC:GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins Gene:LSG1(YGL099W)|FD-Score:10.6|P-value:1.03E-26|Clearance:5.03||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MAK16(YAL025C)|FD-Score:-3.11|P-value:9.47E-4|Clearance:0||SGD DESC:Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus Gene:MSL5(YLR116W)|FD-Score:3.52|P-value:2.19E-4|Clearance:0.06||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:NIC96(YFR002W)|FD-Score:-3.36|P-value:3.93E-4|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NMD3(YHR170W)|FD-Score:5.61|P-value:1.03E-8|Clearance:1.34||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NUS1(YDL193W)|FD-Score:-3.68|P-value:1.18E-4|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:RPB8(YOR224C)|FD-Score:4.27|P-value:9.80E-6|Clearance:0.24||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPG1(YBR079C)|FD-Score:3.2|P-value:6.98E-4|Clearance:0.12||SGD DESC:eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation Gene:RPN6(YDL097C)|FD-Score:-3.8|P-value:7.13E-5|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:SNU56(YDR240C)|FD-Score:3.25|P-value:5.72E-4|Clearance:0.06||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:SRP54(YPR088C)|FD-Score:4.03|P-value:2.82E-5|Clearance:0.23||SGD DESC:Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain Gene:TFC3(YAL001C)|FD-Score:3.8|P-value:7.27E-5|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:ARP7(YPR034W)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.13||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRL1(YHR036W)|FD-Score:3.45|P-value:2.78E-4|Clearance:0.16||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CAB2(YIL083C)|FD-Score:3.53|P-value:2.11E-4|Clearance:0.01||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:CCT3(YJL014W)|FD-Score:3.8|P-value:7.36E-5|Clearance:0.14||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDS1(YBR029C)|FD-Score:-4.56|P-value:2.53E-6|Clearance:0||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:GDI1(YER136W)|FD-Score:3.29|P-value:4.95E-4|Clearance:0.04||SGD DESC:GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins Gene:LSG1(YGL099W)|FD-Score:10.6|P-value:1.03E-26|Clearance:5.03||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MAK16(YAL025C)|FD-Score:-3.11|P-value:9.47E-4|Clearance:0||SGD DESC:Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus Gene:MSL5(YLR116W)|FD-Score:3.52|P-value:2.19E-4|Clearance:0.06||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:NIC96(YFR002W)|FD-Score:-3.36|P-value:3.93E-4|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NMD3(YHR170W)|FD-Score:5.61|P-value:1.03E-8|Clearance:1.34||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NUS1(YDL193W)|FD-Score:-3.68|P-value:1.18E-4|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:RPB8(YOR224C)|FD-Score:4.27|P-value:9.80E-6|Clearance:0.24||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPG1(YBR079C)|FD-Score:3.2|P-value:6.98E-4|Clearance:0.12||SGD DESC:eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation Gene:RPN6(YDL097C)|FD-Score:-3.8|P-value:7.13E-5|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:SNU56(YDR240C)|FD-Score:3.25|P-value:5.72E-4|Clearance:0.06||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:SRP54(YPR088C)|FD-Score:4.03|P-value:2.82E-5|Clearance:0.23||SGD DESC:Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain Gene:TFC3(YAL001C)|FD-Score:3.8|P-value:7.27E-5|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5487158
Download HOP data (tab-delimited text)  (excel)
Gene:AGC1(YPR021C)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:ALP1(YNL270C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication Gene:AST2(YER101C)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication Gene:AUS1(YOR011W)|FD-Score:3.79|P-value:7.58E-5||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:BAG7(YOR134W)|FD-Score:3.73|P-value:9.71E-5||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:BCS1(YDR375C)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases Gene:BIO4(YNR057C)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BXI1(YNL305C)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p Gene:COA1(YIL157C)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:CSM4(YPL200W)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:DMA1(YHR115C)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DYN2(YDR424C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:EAR1(YMR171C)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:EGD2(YHR193C)|FD-Score:5.39|P-value:3.55E-8||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:FAA4(YMR246W)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FRD1(YEL047C)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:GIN4(YDR507C)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:GYP1(YOR070C)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HRB1(YNL004W)|FD-Score:4.27|P-value:9.91E-6||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication Gene:HSP82(YPL240C)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:ISC10(YER180C)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:ISY1(YJR050W)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:MAC1(YMR021C)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAM1(YER106W)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MPC2(YHR162W)|FD-Score:5.74|P-value:4.60E-9||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium Gene:MRI1(YPR118W)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:MRP1(YDR347W)|FD-Score:5.75|P-value:4.35E-9||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein Gene:PKH3(YDR466W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PTP3(YER075C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm Gene:QCR7(YDR529C)|FD-Score:-3.82|P-value:6.54E-5||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RDS1(YCR106W)|FD-Score:5.39|P-value:3.50E-8||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:RNY1(YPL123C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity Gene:RPO41(YFL036W)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:SGF11(YPL047W)|FD-Score:4.29|P-value:9.06E-6||SGD DESC:Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation Gene:SIA1(YOR137C)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose Gene:SOL2(YCR073W-A)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SPO12(YHR152W)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:SWI4(YER111C)|FD-Score:7.04|P-value:9.81E-13||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:SWT1(YOR166C)|FD-Score:3.12|P-value:9.04E-4||SGD DESC:RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain Gene:SYO1(YDL063C)|FD-Score:-3.47|P-value:2.57E-4||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TIP41(YPR040W)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TRR2(YHR106W)|FD-Score:4.3|P-value:8.38E-6||SGD DESC:Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication Gene:TYW1(YPL207W)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron Gene:UBP8(YMR223W)|FD-Score:4.55|P-value:2.66E-6||SGD DESC:Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B Gene:UGA3(YDL170W)|FD-Score:-3.31|P-value:4.65E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:VPS63(YLR261C_d)|FD-Score:5.25|P-value:7.44E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YCR099C(YCR099C_p)|FD-Score:4.35|P-value:6.91E-6||SGD DESC:Putative protein of unknown function Gene:YER034W(YER034W_p)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YGL230C(YGL230C_p)|FD-Score:-3.12|P-value:9.06E-4||SGD DESC:Putative protein of unknown function; non-essential gene Gene:YIP3(YNL044W)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Protein localized to COPII vesicles, proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p Gene:YKR073C(YKR073C_d)|FD-Score:6.73|P-value:8.44E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML082W(YML082W_p)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YMR085W(YMR085W_p)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YNL198C(YNL198C_d)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR025C(YNR025C_d)|FD-Score:-3.12|P-value:9.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YOR072W(YOR072W_d)|FD-Score:4.43|P-value:4.63E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR114W(YOR114W_p)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YPR084W(YPR084W_p)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative protein of unknown function Gene:AGC1(YPR021C)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:ALP1(YNL270C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication Gene:AST2(YER101C)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication Gene:AUS1(YOR011W)|FD-Score:3.79|P-value:7.58E-5||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:BAG7(YOR134W)|FD-Score:3.73|P-value:9.71E-5||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:BCS1(YDR375C)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases Gene:BIO4(YNR057C)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BXI1(YNL305C)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p Gene:COA1(YIL157C)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:CSM4(YPL200W)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:DMA1(YHR115C)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DYN2(YDR424C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:EAR1(YMR171C)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:EGD2(YHR193C)|FD-Score:5.39|P-value:3.55E-8||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:FAA4(YMR246W)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FRD1(YEL047C)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:GIN4(YDR507C)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:GYP1(YOR070C)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HRB1(YNL004W)|FD-Score:4.27|P-value:9.91E-6||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication Gene:HSP82(YPL240C)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:ISC10(YER180C)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:ISY1(YJR050W)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:MAC1(YMR021C)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAM1(YER106W)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MPC2(YHR162W)|FD-Score:5.74|P-value:4.60E-9||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium Gene:MRI1(YPR118W)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:MRP1(YDR347W)|FD-Score:5.75|P-value:4.35E-9||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein Gene:PKH3(YDR466W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PTP3(YER075C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm Gene:QCR7(YDR529C)|FD-Score:-3.82|P-value:6.54E-5||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RDS1(YCR106W)|FD-Score:5.39|P-value:3.50E-8||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:RNY1(YPL123C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity Gene:RPO41(YFL036W)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:SGF11(YPL047W)|FD-Score:4.29|P-value:9.06E-6||SGD DESC:Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation Gene:SIA1(YOR137C)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose Gene:SOL2(YCR073W-A)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SPO12(YHR152W)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:SWI4(YER111C)|FD-Score:7.04|P-value:9.81E-13||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:SWT1(YOR166C)|FD-Score:3.12|P-value:9.04E-4||SGD DESC:RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain Gene:SYO1(YDL063C)|FD-Score:-3.47|P-value:2.57E-4||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TIP41(YPR040W)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TRR2(YHR106W)|FD-Score:4.3|P-value:8.38E-6||SGD DESC:Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication Gene:TYW1(YPL207W)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron Gene:UBP8(YMR223W)|FD-Score:4.55|P-value:2.66E-6||SGD DESC:Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B Gene:UGA3(YDL170W)|FD-Score:-3.31|P-value:4.65E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:VPS63(YLR261C_d)|FD-Score:5.25|P-value:7.44E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YCR099C(YCR099C_p)|FD-Score:4.35|P-value:6.91E-6||SGD DESC:Putative protein of unknown function Gene:YER034W(YER034W_p)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YGL230C(YGL230C_p)|FD-Score:-3.12|P-value:9.06E-4||SGD DESC:Putative protein of unknown function; non-essential gene Gene:YIP3(YNL044W)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Protein localized to COPII vesicles, proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p Gene:YKR073C(YKR073C_d)|FD-Score:6.73|P-value:8.44E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML082W(YML082W_p)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YMR085W(YMR085W_p)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YNL198C(YNL198C_d)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR025C(YNR025C_d)|FD-Score:-3.12|P-value:9.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YOR072W(YOR072W_d)|FD-Score:4.43|P-value:4.63E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR114W(YOR114W_p)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YPR084W(YPR084W_p)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W10.601.03E-265.03LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YHR170W5.611.03E-81.34NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YOR224C4.279.80E-60.24RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YPR088C4.032.82E-50.23SRP54Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain
YAL001C3.807.27E-50.00TFC3Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding
YJL014W3.807.36E-50.14CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YPR034W3.651.29E-40.13ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YIL083C3.532.11E-40.01CAB2Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
YLR116W3.522.19E-40.06MSL5Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA
YHR036W3.452.78E-40.16BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
YER136W3.294.95E-40.04GDI1GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
YDR240C3.255.72E-40.06SNU56Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex
YBR079C3.206.98E-40.12RPG1eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation
YMR211W3.080.001040.03DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YOL127W3.050.001150.06RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER111C7.049.81E-13SWI4DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p
YKR073C_d6.738.44E-12YKR073C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR347W5.754.35E-9MRP1Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein
YHR162W5.744.60E-9MPC2Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium
YCR106W5.393.50E-8RDS1Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide
YHR193C5.393.55E-8EGD2Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes
YLR261C_d5.257.44E-8VPS63_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect
YOR114W_p4.721.16E-6YOR114W_pPutative protein of unknown function; null mutant is viable
YMR223W4.552.66E-6UBP8Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B
YOR072W_d4.434.63E-6YOR072W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive
YCR099C_p4.356.91E-6YCR099C_pPutative protein of unknown function
YHR106W4.308.38E-6TRR2Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication
YPL047W4.299.06E-6SGF11Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation
YNL004W4.279.91E-6HRB1Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication
YNL198C_d4.251.06E-5YNL198C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_403
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1999.06E-54SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.14864960S ribosome export
0.1944.12E-51SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.064935160S ribosome export
0.1927.13E-50SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.08450760S ribosome export
0.1851.10E-46SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.10869660S ribosome export
0.1791.06E-43SGTC_22067254556 147.0 μMChembridge (Fragment library)43989050.12676160S ribosome export
0.1661.16E-37SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.071428660S ribosome export
0.1632.25E-36SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.081081160S ribosome export
0.1561.77E-33SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.07560S ribosome export
0.1526.80E-32SGTC_620180-0423 3.2 μMChemDiv (Drug-like library)301020.07560S ribosome export
0.1483.06E-30SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.068181860S ribosome export
0.1441.17E-28SGTC_8660495-0101 262.0 μMChemDiv (Drug-like library)6356940.086419860S ribosome export
0.1398.27E-27SGTC_490717-0965 32.1 μMChemDiv (Drug-like library)58893490.10256460S ribosome export
0.1381.27E-26SGTC_1983454-2873 16.2 μMChemDiv (Drug-like library)7109570.097222260S ribosome export
0.1351.95E-25SGTC_6450845-0943 5.4 μMChemDiv (Drug-like library)18851060.04597760S ribosome export
0.1345.47E-25SGTC_29839022671 13.0 μMChembridge (Drug-like library)64051610.042105360S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1564D-Arginine115 μM0.54347871070TimTec (Pure natural product library)174.20096-3.83144
SGTC_251melphalan456.52 μM0.283333460612Miscellaneous305.20022-0.11324DNA damage response
SGTC_1796st049173110.4 μM0.28301994134TimTec (Natural product derivative library)181.18854-2.43734
SGTC_2763lisinopril49.32 μM0.259745362119Miscellaneous405.48794-3.67347
SGTC_389tpck16.7 μM0.257576439647Miscellaneous351.847723.38114
SGTC_1696st02559134.79 μM0.2537312787147TimTec (Natural product derivative library)285.276440.49337SWF1 & branched chain AA biosynthesis
SGTC_458nppb133 μM0.2463774549ICCB bioactive library300.309243.52325
SGTC_1888azatryptophan97.5 μM0.2461545354789TimTec (Natural product derivative library)205.21324-2.26334
SGTC_415dibenzyl ketone100 μM0.2448987593Miscellaneous210.271063.29401
SGTC_6530203-001655.2 μM0.234375295302ChemDiv (Drug-like library)289.756743.44212