fumagillin FDA approved compound

(2E,4E,6E,8E)-10-[[(6R,8R,9S,10R)-9-methoxy-10-[(2R,3R)-2-methyl-3-(3-methylbut-2-enyl)oxiran-2-yl]-1-oxaspiro[2.5]octan-8-yl]oxy]-10-oxodeca-2,4,6,8-tetraenoic acid

A methionine aminopeptidase binder.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_404
Screen concentration 50.0 μM
Source Miscellaneous
PubChem CID 12015870
SMILES CC(=CCC1C(O1)(C)C2C(C(CCC23CO3)OC(=O)C=CC=CC=CC=CC(=O)O)OC)C
Standardized SMILES COC1C(CCC2(CO2)C1C3(C)OC3CC=C(C)C)OC(=O)C=CC=CC=CC=CC(=O)O
Molecular weight 458.544
ALogP 3.42
H-bond donor count 1
H-bond acceptor count 7
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) -2.28
% growth inhibition (Hom. pool) -1.52


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 12015870
Download HIP data (tab-delimited text)  (excel)
Gene:BET4(YJL031C)|FD-Score:4.29|P-value:8.84E-6|Clearance:0.26||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:DPM1(YPR183W)|FD-Score:3.55|P-value:1.94E-4|Clearance:0.06||SGD DESC:Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation Gene:ESF2(YNR054C)|FD-Score:3.25|P-value:5.67E-4|Clearance:0||SGD DESC:Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome Gene:HEM3(YDL205C)|FD-Score:-3.14|P-value:8.51E-4|Clearance:0||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:IRA1(YBR140C)|FD-Score:-5.43|P-value:2.89E-8|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MPS2(YGL075C)|FD-Score:3.18|P-value:7.35E-4|Clearance:0.06||SGD DESC:Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p Gene:NAB2(YGL122C)|FD-Score:-4.33|P-value:7.45E-6|Clearance:0||SGD DESC:Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress Gene:NOP14(YDL148C)|FD-Score:3.89|P-value:5.00E-5|Clearance:0.06||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NOP53(YPL146C)|FD-Score:3.59|P-value:1.64E-4|Clearance:0.04||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:PIK1(YNL267W)|FD-Score:3.4|P-value:3.32E-4|Clearance:0.02||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:RPA135(YPR010C)|FD-Score:3.48|P-value:2.47E-4|Clearance:0.08||SGD DESC:RNA polymerase I second largest subunit A135 Gene:RPB8(YOR224C)|FD-Score:-3.5|P-value:2.30E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPS15(YOL040C)|FD-Score:4.04|P-value:2.72E-5|Clearance:0.14||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:SCC2(YDR180W)|FD-Score:3.79|P-value:7.54E-5|Clearance:0.2||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin Gene:SEC13(YLR208W)|FD-Score:3.31|P-value:4.70E-4|Clearance:0.05||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC15(YGL233W)|FD-Score:-3.52|P-value:2.15E-4|Clearance:0||SGD DESC:Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase Gene:TAF13(YML098W)|FD-Score:3.25|P-value:5.72E-4|Clearance:0.07||SGD DESC:TFIID subunit (19 kDa), involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors Gene:TFB1(YDR311W)|FD-Score:3.39|P-value:3.53E-4|Clearance:0.08||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:TIF34(YMR146C)|FD-Score:-3.28|P-value:5.16E-4|Clearance:0||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:TRE2(YOR256C)|FD-Score:3.83|P-value:6.49E-5|Clearance:0.04||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:ULP1(YPL020C)|FD-Score:4.52|P-value:3.04E-6|Clearance:0.26||SGD DESC:Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions Gene:UTP10(YJL109C)|FD-Score:-4.6|P-value:2.07E-6|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance Gene:YGL069C(YGL069C_d)|FD-Score:3.12|P-value:9.14E-4|Clearance:0.05||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:BET4(YJL031C)|FD-Score:4.29|P-value:8.84E-6|Clearance:0.26||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:DPM1(YPR183W)|FD-Score:3.55|P-value:1.94E-4|Clearance:0.06||SGD DESC:Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation Gene:ESF2(YNR054C)|FD-Score:3.25|P-value:5.67E-4|Clearance:0||SGD DESC:Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome Gene:HEM3(YDL205C)|FD-Score:-3.14|P-value:8.51E-4|Clearance:0||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:IRA1(YBR140C)|FD-Score:-5.43|P-value:2.89E-8|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MPS2(YGL075C)|FD-Score:3.18|P-value:7.35E-4|Clearance:0.06||SGD DESC:Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p Gene:NAB2(YGL122C)|FD-Score:-4.33|P-value:7.45E-6|Clearance:0||SGD DESC:Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress Gene:NOP14(YDL148C)|FD-Score:3.89|P-value:5.00E-5|Clearance:0.06||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NOP53(YPL146C)|FD-Score:3.59|P-value:1.64E-4|Clearance:0.04||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:PIK1(YNL267W)|FD-Score:3.4|P-value:3.32E-4|Clearance:0.02||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:RPA135(YPR010C)|FD-Score:3.48|P-value:2.47E-4|Clearance:0.08||SGD DESC:RNA polymerase I second largest subunit A135 Gene:RPB8(YOR224C)|FD-Score:-3.5|P-value:2.30E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPS15(YOL040C)|FD-Score:4.04|P-value:2.72E-5|Clearance:0.14||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:SCC2(YDR180W)|FD-Score:3.79|P-value:7.54E-5|Clearance:0.2||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin Gene:SEC13(YLR208W)|FD-Score:3.31|P-value:4.70E-4|Clearance:0.05||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC15(YGL233W)|FD-Score:-3.52|P-value:2.15E-4|Clearance:0||SGD DESC:Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase Gene:TAF13(YML098W)|FD-Score:3.25|P-value:5.72E-4|Clearance:0.07||SGD DESC:TFIID subunit (19 kDa), involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors Gene:TFB1(YDR311W)|FD-Score:3.39|P-value:3.53E-4|Clearance:0.08||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:TIF34(YMR146C)|FD-Score:-3.28|P-value:5.16E-4|Clearance:0||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:TRE2(YOR256C)|FD-Score:3.83|P-value:6.49E-5|Clearance:0.04||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:ULP1(YPL020C)|FD-Score:4.52|P-value:3.04E-6|Clearance:0.26||SGD DESC:Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions Gene:UTP10(YJL109C)|FD-Score:-4.6|P-value:2.07E-6|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance Gene:YGL069C(YGL069C_d)|FD-Score:3.12|P-value:9.14E-4|Clearance:0.05||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 12015870
Download HOP data (tab-delimited text)  (excel)
Gene:APT2(YDR441C)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication Gene:ASN1(YPR145W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:ATG16(YMR159C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BIO2(YGR286C)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:CIN8(YEL061C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:COX23(YHR116W)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs Gene:CPR1(YDR155C)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; protein abundance increases in response to DNA replication stress Gene:DBP7(YKR024C)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DDR2(YOL052C-A)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DIF1(YLR437C)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein Gene:DUR3(YHL016C)|FD-Score:-3.91|P-value:4.69E-5||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:EAF7(YNL136W)|FD-Score:-4.47|P-value:3.87E-6||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:EDC3(YEL015W)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress Gene:FCY1(YPR062W)|FD-Score:3.96|P-value:3.80E-5||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:GDT1(YBR187W_p)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GEP4(YHR100C)|FD-Score:3.84|P-value:6.23E-5||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GLE2(YER107C)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GTO3(YMR251W)|FD-Score:-3.14|P-value:8.34E-4||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HIS6(YIL020C)|FD-Score:3.91|P-value:4.56E-5||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HPR1(YDR138W)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HSP150(YJL159W)|FD-Score:-3.16|P-value:7.76E-4||SGD DESC:O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication Gene:IMD3(YLR432W)|FD-Score:5.14|P-value:1.41E-7||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:IZH4(YOL101C)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress Gene:KAP123(YER110C)|FD-Score:4.77|P-value:8.99E-7||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:MAP1(YLR244C)|FD-Score:7.95|P-value:9.07E-16||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MCT1(YOR221C)|FD-Score:4.4|P-value:5.41E-6||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MDH2(YOL126C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 Gene:MET17(YLR303W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MRPL35(YDR322W)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSW1(YDR268W)|FD-Score:5.07|P-value:1.99E-7||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MTG1(YMR097C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NMA111(YNL123W)|FD-Score:4.75|P-value:1.04E-6||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:OMS1(YDR316W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PEX3(YDR329C)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p Gene:PSK2(YOL045W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:PST2(YDR032C)|FD-Score:-3.1|P-value:9.62E-4||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:RAD14(YMR201C)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:REG1(YDR028C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIM4(YHL024W)|FD-Score:-3.21|P-value:6.75E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RLM1(YPL089C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication Gene:ROG1(YGL144C)|FD-Score:-3.89|P-value:4.99E-5||SGD DESC:Protein with putative serine active lipase domain; ROG1 has a paralog, YDL109C, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS1B(YML063W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:5.51|P-value:1.77E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTC6(YPL183W-A)|FD-Score:5.18|P-value:1.12E-7||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SCS7(YMR272C)|FD-Score:3.97|P-value:3.59E-5||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SHE4(YOR035C)|FD-Score:4.46|P-value:4.17E-6||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SOL3(YHR163W)|FD-Score:-3.93|P-value:4.28E-5||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:SPC72(YAL047C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SPT21(YMR179W)|FD-Score:4.6|P-value:2.08E-6||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SVL3(YPL032C)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TPN1(YGL186C)|FD-Score:3.88|P-value:5.18E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM12(YML005W)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA Gene:TYW1(YPL207W)|FD-Score:-3.38|P-value:3.69E-4||SGD DESC:Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron Gene:VPS27(YNR006W)|FD-Score:3.87|P-value:5.44E-5||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:YCL076W(YCL076W_d)|FD-Score:-4.34|P-value:7.23E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR061W(YCR061W_p)|FD-Score:4.27|P-value:9.69E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDR514C(YDR514C)|FD-Score:-4.14|P-value:1.77E-5||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YER184C(YER184C_p)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source Gene:YFL052W(YFL052W_p)|FD-Score:4.05|P-value:2.52E-5||SGD DESC:Putative zinc cluster protein that contains a DNA binding domain; computational analysis suggests a role as a transcription factor; null mutant is sensitive to Calcofluor White, low osmolarity, and heat, suggesting a role for YFL052Wp in cell wall integrity Gene:YHR007C-A(YHR007C-A_p)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL068C(YJL068C)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D Gene:YLF2(YHL014C)|FD-Score:-3.87|P-value:5.54E-5||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YML089C(YML089C_d)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage Gene:YMR027W(YMR027W_p)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene Gene:YMR052C-A(YMR052C-A_d)|FD-Score:4.28|P-value:9.26E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL162W(YOL162W_p)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YPL199C(YPL199C_p)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YSR3(YKR053C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication Gene:APT2(YDR441C)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication Gene:ASN1(YPR145W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:ATG16(YMR159C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BIO2(YGR286C)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:CIN8(YEL061C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:COX23(YHR116W)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs Gene:CPR1(YDR155C)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; protein abundance increases in response to DNA replication stress Gene:DBP7(YKR024C)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DDR2(YOL052C-A)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DIF1(YLR437C)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein Gene:DUR3(YHL016C)|FD-Score:-3.91|P-value:4.69E-5||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:EAF7(YNL136W)|FD-Score:-4.47|P-value:3.87E-6||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:EDC3(YEL015W)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress Gene:FCY1(YPR062W)|FD-Score:3.96|P-value:3.80E-5||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:GDT1(YBR187W_p)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GEP4(YHR100C)|FD-Score:3.84|P-value:6.23E-5||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GLE2(YER107C)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GTO3(YMR251W)|FD-Score:-3.14|P-value:8.34E-4||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HIS6(YIL020C)|FD-Score:3.91|P-value:4.56E-5||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HPR1(YDR138W)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HSP150(YJL159W)|FD-Score:-3.16|P-value:7.76E-4||SGD DESC:O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication Gene:IMD3(YLR432W)|FD-Score:5.14|P-value:1.41E-7||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:IZH4(YOL101C)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress Gene:KAP123(YER110C)|FD-Score:4.77|P-value:8.99E-7||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:MAP1(YLR244C)|FD-Score:7.95|P-value:9.07E-16||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MCT1(YOR221C)|FD-Score:4.4|P-value:5.41E-6||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MDH2(YOL126C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 Gene:MET17(YLR303W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MRPL35(YDR322W)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSW1(YDR268W)|FD-Score:5.07|P-value:1.99E-7||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MTG1(YMR097C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NMA111(YNL123W)|FD-Score:4.75|P-value:1.04E-6||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:OMS1(YDR316W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PEX3(YDR329C)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p Gene:PSK2(YOL045W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:PST2(YDR032C)|FD-Score:-3.1|P-value:9.62E-4||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:RAD14(YMR201C)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:REG1(YDR028C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIM4(YHL024W)|FD-Score:-3.21|P-value:6.75E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RLM1(YPL089C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication Gene:ROG1(YGL144C)|FD-Score:-3.89|P-value:4.99E-5||SGD DESC:Protein with putative serine active lipase domain; ROG1 has a paralog, YDL109C, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS1B(YML063W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:5.51|P-value:1.77E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTC6(YPL183W-A)|FD-Score:5.18|P-value:1.12E-7||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SCS7(YMR272C)|FD-Score:3.97|P-value:3.59E-5||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SHE4(YOR035C)|FD-Score:4.46|P-value:4.17E-6||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SOL3(YHR163W)|FD-Score:-3.93|P-value:4.28E-5||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:SPC72(YAL047C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SPT21(YMR179W)|FD-Score:4.6|P-value:2.08E-6||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SVL3(YPL032C)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TPN1(YGL186C)|FD-Score:3.88|P-value:5.18E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM12(YML005W)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA Gene:TYW1(YPL207W)|FD-Score:-3.38|P-value:3.69E-4||SGD DESC:Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron Gene:VPS27(YNR006W)|FD-Score:3.87|P-value:5.44E-5||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:YCL076W(YCL076W_d)|FD-Score:-4.34|P-value:7.23E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR061W(YCR061W_p)|FD-Score:4.27|P-value:9.69E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDR514C(YDR514C)|FD-Score:-4.14|P-value:1.77E-5||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YER184C(YER184C_p)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source Gene:YFL052W(YFL052W_p)|FD-Score:4.05|P-value:2.52E-5||SGD DESC:Putative zinc cluster protein that contains a DNA binding domain; computational analysis suggests a role as a transcription factor; null mutant is sensitive to Calcofluor White, low osmolarity, and heat, suggesting a role for YFL052Wp in cell wall integrity Gene:YHR007C-A(YHR007C-A_p)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL068C(YJL068C)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D Gene:YLF2(YHL014C)|FD-Score:-3.87|P-value:5.54E-5||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YML089C(YML089C_d)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage Gene:YMR027W(YMR027W_p)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene Gene:YMR052C-A(YMR052C-A_d)|FD-Score:4.28|P-value:9.26E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL162W(YOL162W_p)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YPL199C(YPL199C_p)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YSR3(YKR053C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YPL020C4.523.04E-60.26ULP1Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions
YJL031C4.298.84E-60.26BET4Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YOL040C4.042.72E-50.14RPS15Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
YDL148C3.895.00E-50.06NOP14Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
YOR256C3.836.49E-50.04TRE2Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication
YDR180W3.797.54E-50.20SCC2Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin
YPL146C3.591.64E-40.04NOP53Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired
YPR183W3.551.94E-40.06DPM1Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation
YPR010C3.482.47E-40.08RPA135RNA polymerase I second largest subunit A135
YNL267W3.403.32E-40.02PIK1Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
YDR311W3.393.53E-40.08TFB1Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators
YLR208W3.314.70E-40.05SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YNR054C3.255.67E-40.00ESF2Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome
YML098W3.255.72E-40.07TAF13TFIID subunit (19 kDa), involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors
YGL075C3.187.35E-40.06MPS2Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR244C7.959.07E-16MAP1Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p
YIL093C5.511.77E-8RSM25Mitochondrial ribosomal protein of the small subunit
YPL183W-A5.181.12E-7RTC6Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress
YLR432W5.141.41E-7IMD3Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed
YDR268W5.071.99E-7MSW1Mitochondrial tryptophanyl-tRNA synthetase
YER110C4.778.99E-7KAP123Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1
YNL123W4.751.04E-6NMA111Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases
YKR024C4.711.26E-6DBP7Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions
YMR027W_p4.631.85E-6YMR027W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene
YMR179W4.602.08E-6SPT21Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress
YOR035C4.464.17E-6SHE4Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization
YOR221C4.405.41E-6MCT1Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling
YMR052C-A_d4.289.26E-6YMR052C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YCR061W_p4.279.69E-6YCR061W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation
YDR441C4.181.46E-5APT2Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_404
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0921.26E-12SGTC_28849058502 52.0 μMChembridge (Drug-like library)64715540.0674157
0.0856.79E-11SGTC_1743st037606 46.4 μMTimTec (Natural product derivative library)5901420.0319149
0.0722.96E-8SGTC_20255147754 141.0 μMChembridge (Fragment library)3157770.0705882
0.0722.95E-8SGTC_32799140807 49.5 μMChembridge (Drug-like library)455957180.0119048
0.0672.59E-7SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.075
0.0663.24E-7SGTC_7041310-0270 142.0 μMChemDiv (Drug-like library)57180330.0853659
0.0648.34E-7SGTC_2526patulin 37.6 μMMicrosource (Natural product library)46960.0632911
0.0649.70E-7SGTC_23127706453 155.7 μMChembridge (Fragment library)9760980.0421053
0.0631.07E-6SGTC_1772st048439 82.2 μMTimTec (Natural product derivative library)29270680.0697674
0.0631.48E-6SGTC_32009114385 49.5 μMChembridge (Drug-like library)170180300.0689655
0.0612.71E-6SGTC_1731273-0061 2.7 μMChemDiv (Drug-like library)28474140.0224719mitochondrial stress
0.0603.80E-6SGTC_14163966-0321 25.3 μMChemDiv (Drug-like library)5825300.0240964
0.0604.62E-6SGTC_15091326-1131 80.0 μMChemDiv (Drug-like library)7683130.0641026
0.0604.41E-6SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.0697674
0.0589.49E-6SGTC_1773st048542 73.6 μMTimTec (Natural product derivative library)32371380.0813954

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2587piperic acid92.2 μM0.1842115370536Microsource (Natural product library)218.20542.16314
SGTC_1117retinoic acid1.11 μM0.180723444795TimTec (Natural product library)300.435125.55112
SGTC_2571chrysanthemic acid100 μM0.1764712743Microsource (Natural product library)168.232842.17712
SGTC_2552juarezic acid87.02 μM0.1739131549512Microsource (Natural product library)174.19592.39512TSC3-RPN4
SGTC_459nigericin15.3 μM0.15966434230ICCB bioactive library724.961324.538311
SGTC_2625euphol100 μM0.1595746708538Microsource (Natural product library)426.71748.11911
SGTC_2631bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone100 μM0.15730323847180Microsource (Natural product library)399.319283.40914
SGTC_1578josamycin24.2 μM0.1555565282165TimTec (Pure natural product library)827.994962.957316
SGTC_1877wortmannin25 μM0.15534312145ICCB bioactive library428.431861.70508
SGTC_2646retinol96.75 μM0.154762445354Microsource (Natural product library)286.45165.31611