cantharidin


Established Yeast Drug Target : GLC7

Cantharidin is a toxic blistering agent secreted by the Spanish fly of unknown mechanism. In Saccharomyces cerevisiae, cantharidin is thought to act by inhibiting GLC7 (encoding type 1 serine/threonine protein phosphatase) as decreased expression confers hypersensitivity and overexpression confers resisitance (Hoon et al., Nat Chem Biol 4, 2008).

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PubChem MeSH terms: Enzyme Inhibitors;Irritants



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_408
Screen concentration 100.0 μM
Source ICCB bioactive library
PubChem CID 2545
SMILES CC12C3CCC(C1(C(=O)OC2=O)C)O3
Standardized SMILES CC12C3CCC(O3)C1(C)C(=O)OC2=O
Molecular weight 196.1999
ALogP 0.72
H-bond donor count 0
H-bond acceptor count 4
Response signature cell wall signaling

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.12
% growth inhibition (Hom. pool) 5.96


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2545
Download HIP data (tab-delimited text)  (excel)
Gene:BET2(YPR176C)|FD-Score:4.14|P-value:1.75E-5|Clearance:0.01||SGD DESC:Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CDS1(YBR029C)|FD-Score:3.23|P-value:6.21E-4|Clearance:0.02||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:ERG11(YHR007C)|FD-Score:4.4|P-value:5.36E-6|Clearance:0.22||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GLC7(YER133W)|FD-Score:15.7|P-value:6.46E-56|Clearance:8.73||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM13(YDR044W)|FD-Score:4.13|P-value:1.83E-5|Clearance:0.03||SGD DESC:Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p) Gene:HSH49(YOR319W)|FD-Score:3.11|P-value:9.38E-4|Clearance:0.09||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:HTS1(YPR033C)|FD-Score:3.14|P-value:8.45E-4|Clearance:0.03||SGD DESC:Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome Gene:MTR2(YKL186C)|FD-Score:3.9|P-value:4.80E-5|Clearance:0.37||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:MTW1(YAL034W-A)|FD-Score:6.53|P-value:3.27E-11|Clearance:0.1||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly Gene:NUP49(YGL172W)|FD-Score:3.23|P-value:6.20E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:RPC10(YHR143W-A)|FD-Score:-3.35|P-value:4.06E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPL25(YOL127W)|FD-Score:3.42|P-value:3.08E-4|Clearance:0.19||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:SEC13(YLR208W)|FD-Score:6.43|P-value:6.36E-11|Clearance:2.03||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SMC2(YFR031C)|FD-Score:3.98|P-value:3.46E-5|Clearance:0.08||SGD DESC:Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus Gene:TAF8(YML114C)|FD-Score:3.53|P-value:2.10E-4|Clearance:0.1||SGD DESC:TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation Gene:TIF35(YDR429C)|FD-Score:6.98|P-value:1.51E-12|Clearance:0.45||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YAL034C-B(YAL034C-B_d)|FD-Score:3.21|P-value:6.71E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL077W(YBL077W_d)|FD-Score:4.18|P-value:1.47E-5|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:YBR190W(YBR190W_d)|FD-Score:4.1|P-value:2.08E-5|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:BET2(YPR176C)|FD-Score:4.14|P-value:1.75E-5|Clearance:0.01||SGD DESC:Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CDS1(YBR029C)|FD-Score:3.23|P-value:6.21E-4|Clearance:0.02||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:ERG11(YHR007C)|FD-Score:4.4|P-value:5.36E-6|Clearance:0.22||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GLC7(YER133W)|FD-Score:15.7|P-value:6.46E-56|Clearance:8.73||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM13(YDR044W)|FD-Score:4.13|P-value:1.83E-5|Clearance:0.03||SGD DESC:Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p) Gene:HSH49(YOR319W)|FD-Score:3.11|P-value:9.38E-4|Clearance:0.09||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:HTS1(YPR033C)|FD-Score:3.14|P-value:8.45E-4|Clearance:0.03||SGD DESC:Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome Gene:MTR2(YKL186C)|FD-Score:3.9|P-value:4.80E-5|Clearance:0.37||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:MTW1(YAL034W-A)|FD-Score:6.53|P-value:3.27E-11|Clearance:0.1||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly Gene:NUP49(YGL172W)|FD-Score:3.23|P-value:6.20E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:RPC10(YHR143W-A)|FD-Score:-3.35|P-value:4.06E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPL25(YOL127W)|FD-Score:3.42|P-value:3.08E-4|Clearance:0.19||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:SEC13(YLR208W)|FD-Score:6.43|P-value:6.36E-11|Clearance:2.03||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SMC2(YFR031C)|FD-Score:3.98|P-value:3.46E-5|Clearance:0.08||SGD DESC:Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus Gene:TAF8(YML114C)|FD-Score:3.53|P-value:2.10E-4|Clearance:0.1||SGD DESC:TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation Gene:TIF35(YDR429C)|FD-Score:6.98|P-value:1.51E-12|Clearance:0.45||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YAL034C-B(YAL034C-B_d)|FD-Score:3.21|P-value:6.71E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL077W(YBL077W_d)|FD-Score:4.18|P-value:1.47E-5|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:YBR190W(YBR190W_d)|FD-Score:4.1|P-value:2.08E-5|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2545
Download HOP data (tab-delimited text)  (excel)
Gene:ADK1(YDR226W)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:ADO1(YJR105W)|FD-Score:6.56|P-value:2.76E-11||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AIM36(YMR157C)|FD-Score:3.76|P-value:8.43E-5||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:AMD1(YML035C)|FD-Score:3.96|P-value:3.82E-5||SGD DESC:AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools Gene:BCK1(YJL095W)|FD-Score:28.9|P-value:1.59E-183||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BXI1(YNL305C)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p Gene:CIN4(YMR138W)|FD-Score:-3.35|P-value:4.08E-4||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:COX9(YDL067C)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CRG1(YHR209W)|FD-Score:14.2|P-value:5.86E-46||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:CTP1(YBR291C)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family Gene:CYS4(YGR155W)|FD-Score:7.55|P-value:2.15E-14||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAL1(YIR027C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DAL81(YIR023W)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:ELM1(YKL048C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:FYV5(YCL058C)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:GDB1(YPR184W)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GEP4(YHR100C)|FD-Score:-4.47|P-value:3.88E-6||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:HNM1(YGL077C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol Gene:HOS3(YPL116W)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Trichostatin A-insensitive homodimeric histone deacetylase (HDAC) with specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats Gene:HPF1(YOL155C)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:IRC15(YPL017C)|FD-Score:4.54|P-value:2.86E-6||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:KSS1(YGR040W)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains Gene:LCL2(YLR104W_p)|FD-Score:-3.24|P-value:5.95E-4||SGD DESC:Putative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:LEU3(YLR451W)|FD-Score:3.9|P-value:4.91E-5||SGD DESC:Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation Gene:LIP5(YOR196C)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LSM7(YNL147W)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MAP1(YLR244C)|FD-Score:3.87|P-value:5.42E-5||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MHR1(YDR296W)|FD-Score:-3.12|P-value:9.19E-4||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MIH1(YMR036C)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 Gene:MNN1(YER001W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family Gene:MRPL8(YJL063C)|FD-Score:-3.81|P-value:6.92E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NCE102(YPR149W)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs) Gene:PAC10(YGR078C)|FD-Score:5.94|P-value:1.40E-9||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PBS2(YJL128C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PPS1(YBR276C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:RAD14(YMR201C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RLM1(YPL089C)|FD-Score:9.45|P-value:1.67E-21||SGD DESC:MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication Gene:RPA49(YNL248C)|FD-Score:6.01|P-value:9.20E-10||SGD DESC:RNA polymerase I subunit A49 Gene:RPL7B(YPL198W)|FD-Score:3.84|P-value:6.20E-5||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:7.87|P-value:1.79E-15||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP1B(YDL130W)|FD-Score:4.32|P-value:7.88E-6||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RTC6(YPL183W-A)|FD-Score:-6.49|P-value:4.38E-11||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SCY1(YGL083W)|FD-Score:3.78|P-value:7.73E-5||SGD DESC:Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase Gene:SEC72(YLR292C)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SED1(YDR077W)|FD-Score:-3.18|P-value:7.39E-4||SGD DESC:Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites Gene:SLM3(YDL033C)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SLT2(YHR030C)|FD-Score:16.7|P-value:3.41E-63||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SPT3(YDR392W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SSE1(YPL106C)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:SST2(YLR452C)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family Gene:STP1(YDR463W)|FD-Score:-4.4|P-value:5.50E-6||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SWC5(YBR231C)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SWR1(YDR334W)|FD-Score:4|P-value:3.21E-5||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TAT1(YBR069C)|FD-Score:-4.39|P-value:5.74E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TEL1(YBL088C)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585) Gene:TOS1(YBR162C)|FD-Score:4.58|P-value:2.35E-6||SGD DESC:Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C Gene:TPM1(YNL079C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:-4.48|P-value:3.65E-6||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM7(YBR061C)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:VPS72(YDR485C)|FD-Score:3.87|P-value:5.55E-5||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBL071C-B(YBL071C-B_p)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCR064C(YCR064C_d)|FD-Score:4.87|P-value:5.47E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YFR032C-B(YFR032C-B_p)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YJR018W(YJR018W_d)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL096C-B(YKL096C-B_p)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YLR184W(YLR184W_d)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR255C(YLR255C_d)|FD-Score:-5.9|P-value:1.82E-9||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR264C-A(YLR264C-A_p)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Putative protein of unknown function Gene:YML094C-A(YML094C-A_d)|FD-Score:5.05|P-value:2.16E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YNL198C(YNL198C_d)|FD-Score:4.27|P-value:9.97E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR061C(YNR061C_p)|FD-Score:3.84|P-value:6.17E-5||SGD DESC:Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:ADK1(YDR226W)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:ADO1(YJR105W)|FD-Score:6.56|P-value:2.76E-11||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AIM36(YMR157C)|FD-Score:3.76|P-value:8.43E-5||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:AMD1(YML035C)|FD-Score:3.96|P-value:3.82E-5||SGD DESC:AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools Gene:BCK1(YJL095W)|FD-Score:28.9|P-value:1.59E-183||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BXI1(YNL305C)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p Gene:CIN4(YMR138W)|FD-Score:-3.35|P-value:4.08E-4||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:COX9(YDL067C)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CRG1(YHR209W)|FD-Score:14.2|P-value:5.86E-46||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:CTP1(YBR291C)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family Gene:CYS4(YGR155W)|FD-Score:7.55|P-value:2.15E-14||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAL1(YIR027C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DAL81(YIR023W)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:ELM1(YKL048C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:FYV5(YCL058C)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:GDB1(YPR184W)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GEP4(YHR100C)|FD-Score:-4.47|P-value:3.88E-6||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:HNM1(YGL077C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol Gene:HOS3(YPL116W)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Trichostatin A-insensitive homodimeric histone deacetylase (HDAC) with specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats Gene:HPF1(YOL155C)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:IRC15(YPL017C)|FD-Score:4.54|P-value:2.86E-6||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:KSS1(YGR040W)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains Gene:LCL2(YLR104W_p)|FD-Score:-3.24|P-value:5.95E-4||SGD DESC:Putative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:LEU3(YLR451W)|FD-Score:3.9|P-value:4.91E-5||SGD DESC:Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation Gene:LIP5(YOR196C)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LSM7(YNL147W)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MAP1(YLR244C)|FD-Score:3.87|P-value:5.42E-5||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MHR1(YDR296W)|FD-Score:-3.12|P-value:9.19E-4||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MIH1(YMR036C)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 Gene:MNN1(YER001W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family Gene:MRPL8(YJL063C)|FD-Score:-3.81|P-value:6.92E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NCE102(YPR149W)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs) Gene:PAC10(YGR078C)|FD-Score:5.94|P-value:1.40E-9||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PBS2(YJL128C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PPS1(YBR276C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:RAD14(YMR201C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RLM1(YPL089C)|FD-Score:9.45|P-value:1.67E-21||SGD DESC:MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication Gene:RPA49(YNL248C)|FD-Score:6.01|P-value:9.20E-10||SGD DESC:RNA polymerase I subunit A49 Gene:RPL7B(YPL198W)|FD-Score:3.84|P-value:6.20E-5||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:7.87|P-value:1.79E-15||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP1B(YDL130W)|FD-Score:4.32|P-value:7.88E-6||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RTC6(YPL183W-A)|FD-Score:-6.49|P-value:4.38E-11||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SCY1(YGL083W)|FD-Score:3.78|P-value:7.73E-5||SGD DESC:Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase Gene:SEC72(YLR292C)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SED1(YDR077W)|FD-Score:-3.18|P-value:7.39E-4||SGD DESC:Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites Gene:SLM3(YDL033C)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SLT2(YHR030C)|FD-Score:16.7|P-value:3.41E-63||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SPT3(YDR392W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SSE1(YPL106C)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:SST2(YLR452C)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family Gene:STP1(YDR463W)|FD-Score:-4.4|P-value:5.50E-6||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SWC5(YBR231C)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SWR1(YDR334W)|FD-Score:4|P-value:3.21E-5||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TAT1(YBR069C)|FD-Score:-4.39|P-value:5.74E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TEL1(YBL088C)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585) Gene:TOS1(YBR162C)|FD-Score:4.58|P-value:2.35E-6||SGD DESC:Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C Gene:TPM1(YNL079C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:-4.48|P-value:3.65E-6||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM7(YBR061C)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:VPS72(YDR485C)|FD-Score:3.87|P-value:5.55E-5||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBL071C-B(YBL071C-B_p)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCR064C(YCR064C_d)|FD-Score:4.87|P-value:5.47E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YFR032C-B(YFR032C-B_p)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YJR018W(YJR018W_d)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL096C-B(YKL096C-B_p)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YLR184W(YLR184W_d)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR255C(YLR255C_d)|FD-Score:-5.9|P-value:1.82E-9||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR264C-A(YLR264C-A_p)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Putative protein of unknown function Gene:YML094C-A(YML094C-A_d)|FD-Score:5.05|P-value:2.16E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YNL198C(YNL198C_d)|FD-Score:4.27|P-value:9.97E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR061C(YNR061C_p)|FD-Score:3.84|P-value:6.17E-5||SGD DESC:Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YER133W15.706.46E-568.73GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YDR429C6.981.51E-120.45TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YAL034W-A6.533.27E-110.10MTW1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly
YLR208W6.436.36E-112.03SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YHR007C4.405.36E-60.22ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YBL077W_d4.181.47E-50.04YBL077W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C
YPR176C4.141.75E-50.01BET2Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YDR044W4.131.83E-50.03HEM13Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p)
YBR190W_d4.102.08E-50.12YBR190W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
YFR031C3.983.46E-50.08SMC2Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus
YKL186C3.904.80E-50.37MTR2mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA
YML114C3.532.10E-40.10TAF8TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation
YOL127W3.423.08E-40.19RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
YGL172W3.236.20E-43.83E-4NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YBR029C3.236.21E-40.02CDS1Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL095W28.901.59E-183BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YHR030C16.703.41E-63SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YHR209W14.205.86E-46CRG1S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
YPL089C9.451.67E-21RLM1MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication
YDL020C7.871.79E-15RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YGR155W7.552.15E-14CYS4Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria
YJR105W6.562.76E-11ADO1Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle
YNL248C6.019.20E-10RPA49RNA polymerase I subunit A49
YGR078C5.941.40E-9PAC10Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding
YML094C-A_d5.052.16E-7YML094C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO
YCR064C_d4.875.47E-7YCR064C_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31
YLR452C4.621.92E-6SST2GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family
YBR162C4.582.35E-6TOS1Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C
YPL017C4.542.86E-6IRC15Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci
YDL130W4.327.88E-6RPP1BRibosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component

GO enrichment analysis for SGTC_408
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.6230SGTC_422cantharidin 155.0 μMICCB bioactive library25451cell wall signaling
0.5790SGTC_2415hr heat shock (37°C) + cantharidin 155.4 μMICCB bioactive library25451cell wall signaling
0.5470SGTC_2489cantharidin 73.4 μMICCB bioactive library25451cell wall signaling
0.4327.83E-268SGTC_22727805186 180.0 μMChembridge (Fragment library)29560620.0555556cell wall signaling
0.3948.34E-219SGTC_9052-aminofluorene 215.0 μMChemDiv (Drug-like library)15390.047619cell wall signaling
0.3571.61E-177SGTC_500h-89 112.0 μMICCB bioactive library4492410.0289855cell wall signaling
0.3403.39E-159SGTC_6631486-0733 13.9 μMChemDiv (Drug-like library)7224450.0447761cell wall signaling
0.3311.18E-150SGTC_31229126309 49.5 μMChembridge (Drug-like library)175912060.015625cell wall signaling
0.3264.27E-146SGTC_20074011539 71.8 μMChembridge (Fragment library)455953010.12766cell wall signaling
0.3221.50E-142SGTC_20094012297 12.1 μMChembridge (Fragment library)455953070.08cell wall signaling
0.2931.21E-117SGTC_20285153890 36.4 μMChembridge (Fragment library)28309680.0212766cell wall signaling
0.2882.42E-113SGTC_30929117248 49.5 μMChembridge (Drug-like library)171418570.0983607cell wall signaling
0.2873.09E-112SGTC_3600957-0399 1.0 μMChemDiv (Drug-like library)67523010.056338cell wall
0.2844.65E-110SGTC_8251988-2224 89.6 μMChemDiv (Drug-like library)11808590.0461538cell wall signaling
0.2841.66E-109SGTC_21495554786 194.8 μMChembridge (Fragment library)7450630.0377358cell wall signaling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2415hr heat shock (37°C) + cantharidin155.45 μM12545ICCB bioactive library196.199880.71704cell wall signaling
SGTC_2489cantharidin73.42 μM12545ICCB bioactive library196.199880.71704cell wall signaling
SGTC_422cantharidin155 μM12545ICCB bioactive library196.199880.71704cell wall signaling
SGTC_2673kobusone100 μM0.2619056710676Microsource (Natural product library)222.323282.25102
SGTC_25383',7'-epoxycaryophyllan-6-one100 μM0.2380954330530Microsource (Natural product library)236.349862.59902
SGTC_1125tnp002481.33 μM0.23255824207736TimTec (Natural product library)250.376443.49502
SGTC_1570verbenone133 μM0.22222229025TimTec (Pure natural product library)150.217562.08201
SGTC_2558carylophyllene oxide100 μM0.2093026708694Microsource (Natural product library)206.323883.06301
SGTC_1113artemisinin1.18 μM0.2040822240TimTec (Natural product library)282.332181.99805heme biosynthesis & mitochondrial translocase
SGTC_2534helenine20.12 μM0.272724Microsource (Natural product library)232.31813.31102RSC complex & mRNA processing
SGTC_1119parthenolide318.54 nM0.1923085702252TimTec (Natural product library)248.31752.92303TSC3-RPN4