enrofloxacin FDA approved compound

1-cyclopropyl-7-(4-ethylpiperazin-1-yl)-6-fluoro-4-oxoquinoline-3-carboxylic acid

A bacterial gyrase inhibitor.

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PubChem MeSH terms: Antineoplastic Agents



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_411
Screen concentration 100.0 μM
Source Miscellaneous
PubChem CID 71188
SMILES CCN1CCN(CC1)C2=C(C=C3C(=C2)N(C=C(C3=O)C(=O)O)C4CC4)F
Standardized SMILES CCN1CCN(CC1)c2cc3N(C=C(C(=O)O)C(=O)c3cc2F)C4CC4
Molecular weight 359.3947
ALogP -0.72
H-bond donor count 1
H-bond acceptor count 7
Response signature DNA damage response

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.69
% growth inhibition (Hom. pool) -2.87


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 71188
Download HIP data (tab-delimited text)  (excel)
Gene:FCF1(YDR339C)|FD-Score:5.01|P-value:2.69E-7|Clearance:1.68||SGD DESC:Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p Gene:FOL2(YGR267C)|FD-Score:-3.31|P-value:4.63E-4|Clearance:0||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:GLC7(YER133W)|FD-Score:-3.25|P-value:5.68E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:IRA1(YBR140C)|FD-Score:-3.25|P-value:5.82E-4|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:KIN28(YDL108W)|FD-Score:3.1|P-value:9.65E-4|Clearance:0.05||SGD DESC:Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters Gene:LSM5(YER146W)|FD-Score:-3.37|P-value:3.81E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:PRP24(YMR268C)|FD-Score:3.33|P-value:4.34E-4|Clearance:0.23||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RPP1(YHR062C)|FD-Score:-3.31|P-value:4.62E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP17(YDR412W)|FD-Score:-3.15|P-value:8.09E-4|Clearance:0||SGD DESC:Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA Gene:SLN1(YIL147C)|FD-Score:-4.73|P-value:1.12E-6|Clearance:0||SGD DESC:Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators Gene:YAH1(YPL252C)|FD-Score:-4.79|P-value:8.41E-7|Clearance:0||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:FCF1(YDR339C)|FD-Score:5.01|P-value:2.69E-7|Clearance:1.68||SGD DESC:Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p Gene:FOL2(YGR267C)|FD-Score:-3.31|P-value:4.63E-4|Clearance:0||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:GLC7(YER133W)|FD-Score:-3.25|P-value:5.68E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:IRA1(YBR140C)|FD-Score:-3.25|P-value:5.82E-4|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:KIN28(YDL108W)|FD-Score:3.1|P-value:9.65E-4|Clearance:0.05||SGD DESC:Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters Gene:LSM5(YER146W)|FD-Score:-3.37|P-value:3.81E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:PRP24(YMR268C)|FD-Score:3.33|P-value:4.34E-4|Clearance:0.23||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RPP1(YHR062C)|FD-Score:-3.31|P-value:4.62E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP17(YDR412W)|FD-Score:-3.15|P-value:8.09E-4|Clearance:0||SGD DESC:Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA Gene:SLN1(YIL147C)|FD-Score:-4.73|P-value:1.12E-6|Clearance:0||SGD DESC:Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators Gene:YAH1(YPL252C)|FD-Score:-4.79|P-value:8.41E-7|Clearance:0||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 71188
Download HOP data (tab-delimited text)  (excel)
Gene:BOR1(YNL275W)|FD-Score:4.36|P-value:6.42E-6||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:BSC5(YNR069C)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:CHA1(YCL064C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine Gene:ELM1(YKL048C)|FD-Score:-3.24|P-value:6.01E-4||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:FSF1(YOR271C_p)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis Gene:HOP2(YGL033W)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HSC82(YMR186W)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Cytoplasmic chaperone of the Hsp90 family; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication Gene:HUA1(YGR268C)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IVY1(YDR229W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase Gene:LNP1(YHR192W_p)|FD-Score:-3.8|P-value:7.37E-5||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MSH5(YDL154W)|FD-Score:4.33|P-value:7.48E-6||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:NHP6A(YPR052C)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:OM45(YIL136W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress Gene:RAD50(YNL250W)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress Gene:RAD54(YGL163C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RAD55(YDR076W)|FD-Score:5.39|P-value:3.48E-8||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD57(YDR004W)|FD-Score:4.58|P-value:2.31E-6||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RAD59(YDL059C)|FD-Score:4.66|P-value:1.58E-6||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RPS19A(YOL121C)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:SAE2(YGL175C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SHB17(YKR043C)|FD-Score:4.28|P-value:9.44E-6||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SWI6(YLR182W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:YCP4(YCR004C)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YDL009C(YDL009C_p)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YER067C-A(YER067C-A_d)|FD-Score:4.09|P-value:2.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W Gene:YER186C(YER186C_p)|FD-Score:3.89|P-value:4.92E-5||SGD DESC:Putative protein of unknown function Gene:YFR057W(YFR057W_p)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Putative protein of unknown function Gene:YGR001C(YGR001C_p)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Putative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae Gene:YLL054C(YLL054C_p)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene Gene:YLR412C-A(YLR412C-A_p)|FD-Score:4.8|P-value:7.78E-7||SGD DESC:Putative protein of unknown function Gene:YLR456W(YLR456W_p)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Putative pyridoxal 5'-phosphate synthase; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication Gene:YMR155W(YMR155W_p)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YMR252C(YMR252C_p)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene Gene:YNL296W(YNL296W_d)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Gene:BOR1(YNL275W)|FD-Score:4.36|P-value:6.42E-6||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:BSC5(YNR069C)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:CHA1(YCL064C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine Gene:ELM1(YKL048C)|FD-Score:-3.24|P-value:6.01E-4||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:FSF1(YOR271C_p)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis Gene:HOP2(YGL033W)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HSC82(YMR186W)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Cytoplasmic chaperone of the Hsp90 family; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication Gene:HUA1(YGR268C)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IVY1(YDR229W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase Gene:LNP1(YHR192W_p)|FD-Score:-3.8|P-value:7.37E-5||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MSH5(YDL154W)|FD-Score:4.33|P-value:7.48E-6||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:NHP6A(YPR052C)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:OM45(YIL136W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress Gene:RAD50(YNL250W)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress Gene:RAD54(YGL163C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RAD55(YDR076W)|FD-Score:5.39|P-value:3.48E-8||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD57(YDR004W)|FD-Score:4.58|P-value:2.31E-6||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RAD59(YDL059C)|FD-Score:4.66|P-value:1.58E-6||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RPS19A(YOL121C)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:SAE2(YGL175C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SHB17(YKR043C)|FD-Score:4.28|P-value:9.44E-6||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SWI6(YLR182W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:YCP4(YCR004C)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YDL009C(YDL009C_p)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YER067C-A(YER067C-A_d)|FD-Score:4.09|P-value:2.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W Gene:YER186C(YER186C_p)|FD-Score:3.89|P-value:4.92E-5||SGD DESC:Putative protein of unknown function Gene:YFR057W(YFR057W_p)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Putative protein of unknown function Gene:YGR001C(YGR001C_p)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Putative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae Gene:YLL054C(YLL054C_p)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene Gene:YLR412C-A(YLR412C-A_p)|FD-Score:4.8|P-value:7.78E-7||SGD DESC:Putative protein of unknown function Gene:YLR456W(YLR456W_p)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Putative pyridoxal 5'-phosphate synthase; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication Gene:YMR155W(YMR155W_p)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YMR252C(YMR252C_p)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene Gene:YNL296W(YNL296W_d)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR339C5.012.69E-71.68FCF1Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p
YMR268C3.334.34E-40.23PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
YDL108W3.109.65E-40.05KIN28Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters
YOR319W3.050.001140.01HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YMR227C3.040.001190.03TAF7TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation
YLL011W3.010.001300.14SOF1Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p
YOL139C2.870.002040.13CDC33mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth
YJL195C_d2.740.003030.03YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
YGR198W2.720.003300.03YPP1Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene
YKR083C2.680.003660.10DAD2Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YOL097C2.580.004920.09WRS1Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA
YFL024C2.490.006340.03EPL1Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb
YBR079C2.460.006940.01RPG1eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation
YER036C2.450.007110.02ARB1ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p
YFR004W2.430.007450.02RPN11Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR076W5.393.48E-8RAD55Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
YLR412C-A_p4.807.78E-7YLR412C-A_pPutative protein of unknown function
YDL059C4.661.58E-6RAD59Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
YDR004W4.582.31E-6RAD57Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
YNL275W4.366.42E-6BOR1Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1
YDL154W4.337.48E-6MSH5Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans
YKR043C4.289.44E-6SHB17Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus
YPR052C4.231.19E-5NHP6AHigh-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YNR069C4.191.39E-5BSC5Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro
YCR004C4.151.64E-5YCP4Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YGR001C_p4.102.09E-5YGR001C_pPutative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae
YER067C-A_d4.092.11E-5YER067C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
YOL121C4.052.60E-5RPS19AProtein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication
YER186C_p3.894.92E-5YER186C_pPutative protein of unknown function
YGL163C3.551.96E-4RAD54DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress

GO enrichment analysis for SGTC_411
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1971.09E-52SGTC_1079idarubicin 2.4 μMNIH Clinical Collection6363620.122449DNA damage response
0.1785.82E-43SGTC_244nsc-207895 1.3 μMMiscellaneous426400.139241DNA damage response
0.1531.78E-32SGTC_558k064-0027 21.3 μMChemDiv (Drug-like library)50885260.0493827DNA damage response
0.1461.21E-29SGTC_1082camptothecin 424.9 nMTimTec (Natural product library)25380.11828DNA damage response
0.1403.87E-27SGTC_1707st032323 6.3 μMTimTec (Natural product derivative library)31320570.116279DNA damage response
0.1381.54E-26SGTC_248streptozotocin 5.2 mMMiscellaneous53000.0897436DNA damage response
0.1293.41E-23SGTC_32479135149 49.5 μMChembridge (Drug-like library)89344360.0277778DNA damage response
0.1231.97E-21SGTC_8291635-0120 117.0 μMChemDiv (Drug-like library)2850540.027027DNA damage response
0.1165.05E-19SGTC_12900986-0249 10.2 μMChemDiv (Drug-like library)533290.0394737DNA intercalators
0.1071.57E-16SGTC_8241304-0008 100.0 μMChemDiv (Drug-like library)7682450.0645161DNA damage response
0.1024.89E-15SGTC_32549136651 49.5 μMChembridge (Drug-like library)164540300.0704225DNA damage response
0.0961.44E-13SGTC_1105epirubicin 18.1 μMNIH Clinical Collection653480.12037anthracycline transcription coupled DNA repair
0.0921.24E-12SGTC_1930st074634 34.3 μMTimTec (Natural product derivative library)6842530.108108RNA pol III & RNase P/MRP
0.0904.05E-12SGTC_2677mitomycin C 100.0 μMMiscellaneous57460.123596DNA damage response
0.0855.36E-11SGTC_219mitoxanthrone 164.4 μMMiscellaneous42120.0958904DNA intercalators

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1692fluoroquinolonic acid71 μM0.618182483180TimTec (Natural product derivative library)281.6668632.51615
SGTC_399oxolinic acid75 μM0.3968254628Miscellaneous261.230141.27316
SGTC_23296138490200 μM0.301587720019Chembridge (Fragment library)231.247222.12914
SGTC_1919st06711352.3 μM0.2820511268243TimTec (Natural product derivative library)382.721114.20308TSC3-RPN4
SGTC_20024023503131 μM0.2580654736863Chembridge (Fragment library)193.1744231.4303mitochondrial processes
SGTC_14454239-015697.7 μM0.256414008346ChemDiv (Drug-like library)375.4123263.22816
SGTC_2939904457871.43 μM0.2531652653634Chembridge (Drug-like library)377.8403433.43915
SGTC_3202911376149.47 μM0.2531654798415Chembridge (Drug-like library)377.8403433.43915
SGTC_23479049783174.81 μM0.256467988Chembridge (Fragment library)267.754521.95413
SGTC_10174239-0030168 μM0.2432431149593ChemDiv (Drug-like library)406.2919433.78814