st075190

[(8R,9S,13S,14S,17S)-17-hydroxy-13-methyl-6,7,8,9,11,12,14,15,16,17-decahydrocyclopenta[a]phenanthren-3-yl] benzoate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_417
Screen concentration 100.0 μM
Source TimTec (Pure natural product library)
PubChem CID 222757
SMILES CC12CCC3C(C1CCC2O)CCC4=C3C=CC(=C4)OC(=O)C5=CC=CC=C5
Standardized SMILES CC12CCC3C(CCc4cc(OC(=O)c5ccccc5)ccc34)C1CCC2O
Molecular weight 376.488
ALogP 5.51
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.94
% growth inhibition (Hom. pool) 2.53


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 222757
Download HIP data (tab-delimited text)  (excel)
Gene:ARB1(YER036C)|FD-Score:3.41|P-value:3.26E-4|Clearance:0.11||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:GDI1(YER136W)|FD-Score:3.55|P-value:1.93E-4|Clearance:0.14||SGD DESC:GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins Gene:GPI12(YMR281W)|FD-Score:3.3|P-value:4.81E-4|Clearance:0.18||SGD DESC:ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp Gene:NSE5(YML023C)|FD-Score:3.41|P-value:3.20E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:PGS1(YCL004W)|FD-Score:3.12|P-value:9.06E-4|Clearance:0.02||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:SQT1(YIR012W)|FD-Score:4.2|P-value:1.32E-5|Clearance:0.65||SGD DESC:Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:STS1(YIR011C)|FD-Score:3.1|P-value:9.73E-4|Clearance:0.05||SGD DESC:Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation Gene:YGR114C(YGR114C_d)|FD-Score:-3.75|P-value:8.77E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:ARB1(YER036C)|FD-Score:3.41|P-value:3.26E-4|Clearance:0.11||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:GDI1(YER136W)|FD-Score:3.55|P-value:1.93E-4|Clearance:0.14||SGD DESC:GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins Gene:GPI12(YMR281W)|FD-Score:3.3|P-value:4.81E-4|Clearance:0.18||SGD DESC:ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp Gene:NSE5(YML023C)|FD-Score:3.41|P-value:3.20E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:PGS1(YCL004W)|FD-Score:3.12|P-value:9.06E-4|Clearance:0.02||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:SQT1(YIR012W)|FD-Score:4.2|P-value:1.32E-5|Clearance:0.65||SGD DESC:Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:STS1(YIR011C)|FD-Score:3.1|P-value:9.73E-4|Clearance:0.05||SGD DESC:Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation Gene:YGR114C(YGR114C_d)|FD-Score:-3.75|P-value:8.77E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 222757
Download HOP data (tab-delimited text)  (excel)
Gene:AAC3(YBR085W)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:AFT1(YGL071W)|FD-Score:-3.13|P-value:8.77E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARG81(YML099C)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ASF1(YJL115W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:ATG8(YBL078C)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BAP3(YDR046C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication Gene:BCK1(YJL095W)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BNA1(YJR025C)|FD-Score:3.9|P-value:4.84E-5||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BTS1(YPL069C)|FD-Score:8.17|P-value:1.60E-16||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CIN4(YMR138W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CLN1(YMR199W)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:COX23(YHR116W)|FD-Score:3.09|P-value:9.84E-4||SGD DESC:Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs Gene:CTL1(YMR180C)|FD-Score:4.34|P-value:6.98E-6||SGD DESC:RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm Gene:ECM2(YBR065C)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:GDE1(YPL110C)|FD-Score:-3.24|P-value:5.97E-4||SGD DESC:Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes Gene:HSP82(YPL240C)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:HUL4(YJR036C)|FD-Score:-5.13|P-value:1.41E-7||SGD DESC:Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex Gene:MDM38(YOL027C)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MRS1(YIR021W)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA Gene:NHP6A(YPR052C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:PAU11(YGL261C_p)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PDA1(YER178W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:PET18(YCR020C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin Gene:PHO88(YBR106W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:POR2(YIL114C)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:RCR2(YDR003W)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Vacuolar protein; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication Gene:REC104(YHR157W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RGP1(YDR137W)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:ROM2(YLR371W)|FD-Score:-3.11|P-value:9.24E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPN4(YDL020C)|FD-Score:9.13|P-value:3.33E-20||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS1A(YLR441C)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RSF1(YMR030W)|FD-Score:3.74|P-value:9.03E-5||SGD DESC:Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes Gene:SER33(YIL074C)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SLX1(YBR228W)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SOV1(YMR066W)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Mitochondrial protein of unknown function Gene:SPI1(YER150W)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SPR28(YDR218C)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; meiotic septin expressed at high levels during meiotic divisions and ascospore formation Gene:THI74(YDR438W)|FD-Score:-3.85|P-value:5.81E-5||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:TMN2(YDR107C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments Gene:TRX1(YLR043C)|FD-Score:3.81|P-value:7.02E-5||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:UBC8(YEL012W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:UBP1(YDL122W)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:VPS68(YOL129W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YDR179W-A(YDR179W-A_p)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Putative protein of unknown function Gene:YGL138C(YGL138C_p)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YHR033W(YHR033W_p)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YHR033W has a paralog, PRO1, that arose from the whole genome duplication Gene:YKL118W(YKL118W_d)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR428C(YLR428C_d)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YML002W(YML002W_p)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YMR155W(YMR155W_p)|FD-Score:3.23|P-value:6.30E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YNL024C(YNL024C_p)|FD-Score:4.36|P-value:6.44E-6||SGD DESC:Putative methyltransferase; has seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; despite similarity to methyltransferases, null mutant does not display alterations in lysine methylation pattern Gene:YNL046W(YNL046W_p)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Putative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YPL245W(YPL245W_p)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YPR130C(YPR130C_d)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR197C(YPR197C_d)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:AAC3(YBR085W)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:AFT1(YGL071W)|FD-Score:-3.13|P-value:8.77E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARG81(YML099C)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ASF1(YJL115W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:ATG8(YBL078C)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BAP3(YDR046C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication Gene:BCK1(YJL095W)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BNA1(YJR025C)|FD-Score:3.9|P-value:4.84E-5||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BTS1(YPL069C)|FD-Score:8.17|P-value:1.60E-16||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CIN4(YMR138W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CLN1(YMR199W)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:COX23(YHR116W)|FD-Score:3.09|P-value:9.84E-4||SGD DESC:Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs Gene:CTL1(YMR180C)|FD-Score:4.34|P-value:6.98E-6||SGD DESC:RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm Gene:ECM2(YBR065C)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:GDE1(YPL110C)|FD-Score:-3.24|P-value:5.97E-4||SGD DESC:Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes Gene:HSP82(YPL240C)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:HUL4(YJR036C)|FD-Score:-5.13|P-value:1.41E-7||SGD DESC:Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex Gene:MDM38(YOL027C)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MRS1(YIR021W)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA Gene:NHP6A(YPR052C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:PAU11(YGL261C_p)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PDA1(YER178W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:PET18(YCR020C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin Gene:PHO88(YBR106W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:POR2(YIL114C)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:RCR2(YDR003W)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Vacuolar protein; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication Gene:REC104(YHR157W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RGP1(YDR137W)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:ROM2(YLR371W)|FD-Score:-3.11|P-value:9.24E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPN4(YDL020C)|FD-Score:9.13|P-value:3.33E-20||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS1A(YLR441C)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RSF1(YMR030W)|FD-Score:3.74|P-value:9.03E-5||SGD DESC:Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes Gene:SER33(YIL074C)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SLX1(YBR228W)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SOV1(YMR066W)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Mitochondrial protein of unknown function Gene:SPI1(YER150W)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SPR28(YDR218C)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; meiotic septin expressed at high levels during meiotic divisions and ascospore formation Gene:THI74(YDR438W)|FD-Score:-3.85|P-value:5.81E-5||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:TMN2(YDR107C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments Gene:TRX1(YLR043C)|FD-Score:3.81|P-value:7.02E-5||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:UBC8(YEL012W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:UBP1(YDL122W)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:VPS68(YOL129W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YDR179W-A(YDR179W-A_p)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Putative protein of unknown function Gene:YGL138C(YGL138C_p)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YHR033W(YHR033W_p)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YHR033W has a paralog, PRO1, that arose from the whole genome duplication Gene:YKL118W(YKL118W_d)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR428C(YLR428C_d)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YML002W(YML002W_p)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YMR155W(YMR155W_p)|FD-Score:3.23|P-value:6.30E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YNL024C(YNL024C_p)|FD-Score:4.36|P-value:6.44E-6||SGD DESC:Putative methyltransferase; has seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; despite similarity to methyltransferases, null mutant does not display alterations in lysine methylation pattern Gene:YNL046W(YNL046W_p)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Putative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YPL245W(YPL245W_p)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YPR130C(YPR130C_d)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR197C(YPR197C_d)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIR012W4.201.32E-50.65SQT1Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress
YER136W3.551.93E-40.14GDI1GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
YML023C3.413.20E-40.00NSE5Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
YER036C3.413.26E-40.11ARB1ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p
YMR281W3.304.81E-40.18GPI12ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp
YCL004W3.129.06E-40.02PGS1Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis
YIR011C3.109.73E-40.05STS1Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation
YAR019C3.050.001160.06CDC15Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress
YOR057W2.990.001390.02SGT1Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress
YGL065C2.970.001490.03ALG2Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol
YOR250C2.940.001630.02CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YDL147W2.920.001720.17RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YPL252C2.750.002950.05YAH1Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
YFR027W2.700.003440.00ECO1Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress
YNR003C2.700.003470.03RPC34RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL020C9.133.33E-20RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YPL069C8.171.60E-16BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YNL024C_p4.366.44E-6YNL024C_pPutative methyltransferase; has seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; despite similarity to methyltransferases, null mutant does not display alterations in lysine methylation pattern
YMR180C4.346.98E-6CTL1RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm
YPL245W_p4.191.40E-5YPL245W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm
YPR130C_d4.171.50E-5YPR130C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBR065C4.022.93E-5ECM2Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p
YML099C4.013.05E-5ARG81Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p
YIL114C3.934.18E-5POR2Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication
YJR025C3.904.84E-5BNA13-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
YLR043C3.817.02E-5TRX1Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress
YMR030W3.749.03E-5RSF1Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes
YML002W_p3.701.06E-4YML002W_pPutative protein of unknown function; expression induced by heat and by calcium shortage
YHR157W3.701.06E-4REC104Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination
YDR046C3.611.51E-4BAP3Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication

GO enrichment analysis for SGTC_417
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1623.53E-36SGTC_32799140807 49.5 μMChembridge (Drug-like library)455957180.0657895
0.1581.84E-34SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.123288
0.1351.55E-25SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.123288
0.1292.41E-23SGTC_29187977171 22.1 μMChembridge (Drug-like library)22364830.160494
0.1279.52E-23SGTC_23859074692 200.0 μMChembridge (Fragment library)170469060.102941
0.1233.04E-21SGTC_6371181-0519 7.3 μMChemDiv (Drug-like library)62520910.0864198fatty acid desaturase (OLE1)
0.1233.08E-21SGTC_575k072-0246 11.9 μMChemDiv (Drug-like library)67967790.123596
0.1126.73E-18SGTC_1370438-0332 85.4 μMChemDiv (Drug-like library)20633930.0857143ubiquinone biosynthesis & proteasome
0.1111.07E-17SGTC_553k007-0256 45.7 μMChemDiv (Drug-like library)62870000.0779221heme biosynthesis & mitochondrial translocase
0.1111.10E-17SGTC_2735sertaconazole 140.0 nMMiscellaneous658630.0594059plasma membrane duress
0.1086.96E-17SGTC_1026k007-1105 30.6 μMChemDiv (Drug-like library)70667940.097561ERAD & cell cycle
0.1072.09E-16SGTC_489paxilline 115.0 μMICCB bioactive library1050080.14PDR1
0.1063.48E-16SGTC_14173966-0327 90.4 μMChemDiv (Drug-like library)6511180.116279
0.1063.79E-16SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.142857
0.1054.65E-16SGTC_1420335-0849 62.7 μMChemDiv (Drug-like library)38890430.103448ubiquinone biosynthesis & proteasome

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_231b-estradiol524 μM0.603448450Miscellaneous272.381963.83822
SGTC_1587estradiol valerate56.1 μM0.45945913791TimTec (Pure natural product library)356.498385.79613
SGTC_416estradiol valerate100 μM0.45945913791TimTec (Pure natural product library)356.498385.79613
SGTC_406estrone100 μM0.4029855870Miscellaneous270.366083.93812
SGTC_1519esterone74 μM0.402985698TimTec (Pure natural product library)270.366083.93812DNA intercalators
SGTC_1767st04499270.3 μM0.3661979600693TimTec (Natural product derivative library)284.395963.50123
SGTC_1977st07072065.3 μM0.323944682188TimTec (Natural product derivative library)306.312064.30904
SGTC_1974st06944826.9 μM0.36323587TimTec (Natural product derivative library)305.454943.36923
SGTC_1586deoxycorticosterone acetate53.7 μM0.2560985952TimTec (Pure natural product library)372.497783.56704
SGTC_25303-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene92.62 μM0.2463776708760Microsource (Natural product library)216.31873.35611
SGTC_1976st07060963.5 μM0.24359682556TimTec (Natural product derivative library)314.719844.44204