splitomicin

1,2-dihydrobenzo[f]chromen-3-one

Sir2p (silent information regulator) and HDAC inhibitor.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_424
Screen concentration 100.0 mM
Source Miscellaneous
PubChem CID 5269
SMILES C1CC(=O)OC2=C1C3=CC=CC=C3C=C2
Standardized SMILES O=C1CCc2c(O1)ccc3ccccc23
Molecular weight 198.2173
ALogP 2.83
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) 3.65


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5269
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:3.18|P-value:7.43E-4|Clearance:0.01||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:BRL1(YHR036W)|FD-Score:-4.52|P-value:3.08E-6|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CCT4(YDL143W)|FD-Score:3.21|P-value:6.63E-4|Clearance:0.02||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC123(YLR215C)|FD-Score:-3.32|P-value:4.45E-4|Clearance:0||SGD DESC:Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein Gene:CDC13(YDL220C)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.04||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:ECM16(YMR128W)|FD-Score:3.83|P-value:6.51E-5|Clearance:0||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:ERV1(YGR029W)|FD-Score:-3.18|P-value:7.46E-4|Clearance:0||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:FIP1(YJR093C)|FD-Score:4.78|P-value:8.56E-7|Clearance:0.5||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:GPI15(YNL038W)|FD-Score:-3.21|P-value:6.64E-4|Clearance:0||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein Gene:NAB2(YGL122C)|FD-Score:4.29|P-value:9.01E-6|Clearance:0.22||SGD DESC:Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress Gene:RSC9(YML127W)|FD-Score:-3.12|P-value:9.17E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC14(YMR079W)|FD-Score:3.82|P-value:6.56E-5|Clearance:0.43||SGD DESC:Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs Gene:SEC61(YLR378C)|FD-Score:3.11|P-value:9.24E-4|Clearance:0.05||SGD DESC:Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER Gene:TAF11(YML015C)|FD-Score:3.35|P-value:3.98E-4|Clearance:0.14||SGD DESC:TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors Gene:UTP8(YGR128C)|FD-Score:3.17|P-value:7.57E-4|Clearance:0.06||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL163W(YDL163W_d)|FD-Score:-3.32|P-value:4.43E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:YGR114C(YGR114C_d)|FD-Score:3.19|P-value:7.14E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YOR169C(YOR169C_d)|FD-Score:4.07|P-value:2.33E-5|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GLN4/YOR168W Gene:ACT1(YFL039C)|FD-Score:3.18|P-value:7.43E-4|Clearance:0.01||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:BRL1(YHR036W)|FD-Score:-4.52|P-value:3.08E-6|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CCT4(YDL143W)|FD-Score:3.21|P-value:6.63E-4|Clearance:0.02||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC123(YLR215C)|FD-Score:-3.32|P-value:4.45E-4|Clearance:0||SGD DESC:Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein Gene:CDC13(YDL220C)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.04||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:ECM16(YMR128W)|FD-Score:3.83|P-value:6.51E-5|Clearance:0||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:ERV1(YGR029W)|FD-Score:-3.18|P-value:7.46E-4|Clearance:0||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:FIP1(YJR093C)|FD-Score:4.78|P-value:8.56E-7|Clearance:0.5||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:GPI15(YNL038W)|FD-Score:-3.21|P-value:6.64E-4|Clearance:0||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein Gene:NAB2(YGL122C)|FD-Score:4.29|P-value:9.01E-6|Clearance:0.22||SGD DESC:Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress Gene:RSC9(YML127W)|FD-Score:-3.12|P-value:9.17E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC14(YMR079W)|FD-Score:3.82|P-value:6.56E-5|Clearance:0.43||SGD DESC:Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs Gene:SEC61(YLR378C)|FD-Score:3.11|P-value:9.24E-4|Clearance:0.05||SGD DESC:Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER Gene:TAF11(YML015C)|FD-Score:3.35|P-value:3.98E-4|Clearance:0.14||SGD DESC:TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors Gene:UTP8(YGR128C)|FD-Score:3.17|P-value:7.57E-4|Clearance:0.06||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL163W(YDL163W_d)|FD-Score:-3.32|P-value:4.43E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:YGR114C(YGR114C_d)|FD-Score:3.19|P-value:7.14E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YOR169C(YOR169C_d)|FD-Score:4.07|P-value:2.33E-5|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GLN4/YOR168W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5269
Download HOP data (tab-delimited text)  (excel)
Gene:ADH7(YCR105W)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase with broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance Gene:BNA6(YFR047C)|FD-Score:3.86|P-value:5.71E-5||SGD DESC:Quinolinate phosphoribosyl transferase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BTS1(YPL069C)|FD-Score:-3.9|P-value:4.78E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD19(YJL188C_d)|FD-Score:-3.1|P-value:9.56E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CNN1(YFR046C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T Gene:COT1(YOR316C)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication Gene:DDP1(YOR163W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress Gene:DID4(YKL002W)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DNF1(YER166W)|FD-Score:-6.84|P-value:3.85E-12||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:DOA1(YKL213C)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DXO1(YDR370C_p)|FD-Score:3.09|P-value:9.89E-4||SGD DESC:Protein with decapping and 5′-3′ exoRNase activity; similar to Rai1p Gene:EDE1(YBL047C)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:EFM2(YBR271W)|FD-Score:5.84|P-value:2.67E-9||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis Gene:EMP47(YFL048C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:ENV7(YPL236C)|FD-Score:4.39|P-value:5.65E-6||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:ERD1(YDR414C)|FD-Score:4.84|P-value:6.50E-7||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ERF2(YLR246W)|FD-Score:-3.32|P-value:4.51E-4||SGD DESC:Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole Gene:FMP52(YER004W)|FD-Score:-3.99|P-value:3.28E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FYV1(YDR024W_d)|FD-Score:3.99|P-value:3.26E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:HCS1(YKL017C)|FD-Score:-3.95|P-value:3.94E-5||SGD DESC:Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities Gene:HEK2(YBL032W)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K Gene:HIS6(YIL020C)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HMF1(YER057C)|FD-Score:8.58|P-value:4.68E-18||SGD DESC:Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro Gene:HOS1(YPR068C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:HOS2(YGL194C)|FD-Score:-5.15|P-value:1.29E-7||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:HST2(YPL015C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HUG1(YML058W-A)|FD-Score:-3.21|P-value:6.63E-4||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:IST1(YNL265C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:LDH1(YBR204C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) Gene:MBP1(YDL056W)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MRPL22(YNL177C)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MXR1(YER042W)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:NAT1(YDL040C)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NNF2(YGR089W)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:NPT1(YOR209C)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:PFK1(YGR240C)|FD-Score:-4.34|P-value:7.23E-6||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PLB2(YMR006C)|FD-Score:7.91|P-value:1.30E-15||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine Gene:PSD2(YGR170W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine Gene:PUF3(YLL013C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:PUT2(YHR037W)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:REV1(YOR346W)|FD-Score:-3.52|P-value:2.16E-4||SGD DESC:Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RFU1(YLR073C)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RGA2(YDR379W)|FD-Score:5.32|P-value:5.17E-8||SGD DESC:GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p Gene:RIM20(YOR275C)|FD-Score:-3.57|P-value:1.82E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RIP1(YEL024W)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly Gene:RPP2A(YOL039W)|FD-Score:4.44|P-value:4.43E-6||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RTG1(YOL067C)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RUP1(YOR138C)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SIL1(YOL031C)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SLI1(YGR212W)|FD-Score:3.9|P-value:4.83E-5||SGD DESC:N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin Gene:SOD1(YJR104C)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPG1(YGR236C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SPR1(YOR190W)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:SUN4(YNL066W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Cell wall protein related to glucanases, possibly involved in cell wall septation; member of the SUN family Gene:TRX1(YLR043C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:TUP1(YCR084C)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:General repressor of transcription, forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes Gene:UBP2(YOR124C)|FD-Score:4.43|P-value:4.80E-6||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity Gene:UTR1(YJR049C)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VPS4(YPR173C)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS64(YDR200C)|FD-Score:-3.89|P-value:5.04E-5||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:YAL044W-A(YAL044W-A_p)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein Gene:YAR028W(YAR028W_p)|FD-Score:5.38|P-value:3.82E-8||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YBR296C-A(YBR296C-A_p)|FD-Score:-4.22|P-value:1.22E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCR087W(YCR087W_d)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDR029W(YDR029W_d)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR186C(YDR186C)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR222W(YDR222W_p)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YER152C(YER152C)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene Gene:YER184C(YER184C_p)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source Gene:YER187W(YER187W_p)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Putative protein of unknown function; induced in respiratory-deficient cells Gene:YFR032C-B(YFR032C-B_p)|FD-Score:5.49|P-value:1.98E-8||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGR139W(YGR139W_d)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHL042W(YHL042W_p)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:YHR180W(YHR180W_d)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIP4(YGL198W)|FD-Score:5.11|P-value:1.64E-7||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJR038C(YJR038C_d)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL118W(YKL118W_d)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKR005C(YKR005C_p)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Putative protein of unknown function Gene:YLR252W(YLR252W_d)|FD-Score:3.77|P-value:8.07E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YLR312C(YLR312C_p)|FD-Score:-3.87|P-value:5.37E-5||SGD DESC:Putative protein of unknown function Gene:YML007C-A(YML007C-A_p)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YNL146W(YNL146W_p)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene Gene:YOR238W(YOR238W_p)|FD-Score:4.46|P-value:4.01E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YOR309C(YOR309C_d)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPR123C(YPR123C_d)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YSP1(YHR155W)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:YSY6(YBR162W-A)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Protein whose expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion Gene:ADH7(YCR105W)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase with broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance Gene:BNA6(YFR047C)|FD-Score:3.86|P-value:5.71E-5||SGD DESC:Quinolinate phosphoribosyl transferase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BTS1(YPL069C)|FD-Score:-3.9|P-value:4.78E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD19(YJL188C_d)|FD-Score:-3.1|P-value:9.56E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CNN1(YFR046C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T Gene:COT1(YOR316C)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication Gene:DDP1(YOR163W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress Gene:DID4(YKL002W)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DNF1(YER166W)|FD-Score:-6.84|P-value:3.85E-12||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:DOA1(YKL213C)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DXO1(YDR370C_p)|FD-Score:3.09|P-value:9.89E-4||SGD DESC:Protein with decapping and 5′-3′ exoRNase activity; similar to Rai1p Gene:EDE1(YBL047C)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:EFM2(YBR271W)|FD-Score:5.84|P-value:2.67E-9||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis Gene:EMP47(YFL048C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:ENV7(YPL236C)|FD-Score:4.39|P-value:5.65E-6||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:ERD1(YDR414C)|FD-Score:4.84|P-value:6.50E-7||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ERF2(YLR246W)|FD-Score:-3.32|P-value:4.51E-4||SGD DESC:Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole Gene:FMP52(YER004W)|FD-Score:-3.99|P-value:3.28E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FYV1(YDR024W_d)|FD-Score:3.99|P-value:3.26E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:HCS1(YKL017C)|FD-Score:-3.95|P-value:3.94E-5||SGD DESC:Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities Gene:HEK2(YBL032W)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K Gene:HIS6(YIL020C)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HMF1(YER057C)|FD-Score:8.58|P-value:4.68E-18||SGD DESC:Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro Gene:HOS1(YPR068C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:HOS2(YGL194C)|FD-Score:-5.15|P-value:1.29E-7||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:HST2(YPL015C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HUG1(YML058W-A)|FD-Score:-3.21|P-value:6.63E-4||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:IST1(YNL265C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:LDH1(YBR204C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) Gene:MBP1(YDL056W)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MRPL22(YNL177C)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MXR1(YER042W)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:NAT1(YDL040C)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NNF2(YGR089W)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:NPT1(YOR209C)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:PFK1(YGR240C)|FD-Score:-4.34|P-value:7.23E-6||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PLB2(YMR006C)|FD-Score:7.91|P-value:1.30E-15||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine Gene:PSD2(YGR170W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine Gene:PUF3(YLL013C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:PUT2(YHR037W)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:REV1(YOR346W)|FD-Score:-3.52|P-value:2.16E-4||SGD DESC:Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RFU1(YLR073C)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RGA2(YDR379W)|FD-Score:5.32|P-value:5.17E-8||SGD DESC:GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p Gene:RIM20(YOR275C)|FD-Score:-3.57|P-value:1.82E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RIP1(YEL024W)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly Gene:RPP2A(YOL039W)|FD-Score:4.44|P-value:4.43E-6||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RTG1(YOL067C)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RUP1(YOR138C)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SIL1(YOL031C)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SLI1(YGR212W)|FD-Score:3.9|P-value:4.83E-5||SGD DESC:N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin Gene:SOD1(YJR104C)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPG1(YGR236C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SPR1(YOR190W)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:SUN4(YNL066W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Cell wall protein related to glucanases, possibly involved in cell wall septation; member of the SUN family Gene:TRX1(YLR043C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:TUP1(YCR084C)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:General repressor of transcription, forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes Gene:UBP2(YOR124C)|FD-Score:4.43|P-value:4.80E-6||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity Gene:UTR1(YJR049C)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VPS4(YPR173C)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS64(YDR200C)|FD-Score:-3.89|P-value:5.04E-5||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:YAL044W-A(YAL044W-A_p)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein Gene:YAR028W(YAR028W_p)|FD-Score:5.38|P-value:3.82E-8||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YBR296C-A(YBR296C-A_p)|FD-Score:-4.22|P-value:1.22E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCR087W(YCR087W_d)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDR029W(YDR029W_d)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR186C(YDR186C)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR222W(YDR222W_p)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YER152C(YER152C)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene Gene:YER184C(YER184C_p)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source Gene:YER187W(YER187W_p)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Putative protein of unknown function; induced in respiratory-deficient cells Gene:YFR032C-B(YFR032C-B_p)|FD-Score:5.49|P-value:1.98E-8||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGR139W(YGR139W_d)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHL042W(YHL042W_p)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:YHR180W(YHR180W_d)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIP4(YGL198W)|FD-Score:5.11|P-value:1.64E-7||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJR038C(YJR038C_d)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL118W(YKL118W_d)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKR005C(YKR005C_p)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Putative protein of unknown function Gene:YLR252W(YLR252W_d)|FD-Score:3.77|P-value:8.07E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YLR312C(YLR312C_p)|FD-Score:-3.87|P-value:5.37E-5||SGD DESC:Putative protein of unknown function Gene:YML007C-A(YML007C-A_p)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YNL146W(YNL146W_p)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene Gene:YOR238W(YOR238W_p)|FD-Score:4.46|P-value:4.01E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YOR309C(YOR309C_d)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPR123C(YPR123C_d)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YSP1(YHR155W)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:YSY6(YBR162W-A)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Protein whose expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJR093C4.788.56E-70.50FIP1Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p
YGL122C4.299.01E-60.22NAB2Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress
YOR169C_d4.072.33E-50.25YOR169C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GLN4/YOR168W
YMR128W3.836.51E-50.00ECM16Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis
YMR079W3.826.56E-50.43SEC14Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
YDL220C3.393.45E-40.04CDC13Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation
YML015C3.353.98E-40.14TAF11TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors
YDL143W3.216.63E-40.02CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YGR114C_d3.197.14E-40.01YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YFL039C3.187.43E-40.01ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YGR128C3.177.57E-40.06UTP8Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YLR378C3.119.24E-40.05SEC61Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER
YOR282W_d3.070.001080.03YOR282W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
YIL004C3.040.001190.01BET1Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins
YDL140C3.030.001220.01RPO21RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER057C8.584.68E-18HMF1Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro
YMR006C7.911.30E-15PLB2Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine
YBR271W5.842.67E-9EFM2S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis
YFR032C-B_p5.491.98E-8YFR032C-B_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YAR028W_p5.383.82E-8YAR028W_pPutative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
YDR379W5.325.17E-8RGA2GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p
YGL198W5.111.64E-7YIP4Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport
YDR414C4.846.50E-7ERD1Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p)
YKL213C4.691.38E-6DOA1WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress
YOR238W_p4.464.01E-6YOR238W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YOL039W4.444.43E-6RPP2ARibosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm
YOR124C4.434.80E-6UBP2Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity
YPL236C4.395.65E-6ENV7Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16)
YOR190W4.241.14E-5SPR1Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance
YER187W_p4.201.36E-5YER187W_pPutative protein of unknown function; induced in respiratory-deficient cells

GO enrichment analysis for SGTC_424
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0621.74E-6SGTC_22277312221 64.1 μMChembridge (Fragment library)8871880.0877193
0.0561.59E-5SGTC_1876myriocin 605.0 nMMiscellaneous3011190.0142857
0.0534.85E-5SGTC_1060092-0003 11.6 μMChemDiv (Drug-like library)10721480.134615copper-dependent oxidative stress
0.0519.15E-5SGTC_2507purpurin 79.7 μMMicrosource (Natural product library)66830.155556copper-dependent oxidative stress
0.0501.07E-4SGTC_1130099-0221 2.5 μMChemDiv (Drug-like library)892190.0769231
0.0501.39E-4SGTC_1736st036774 51.0 μMTimTec (Natural product derivative library)12722470.114754
0.0481.93E-4SGTC_9433253-1379 105.0 μMChemDiv (Drug-like library)6684640.105263
0.0482.09E-4SGTC_21966573537 42.7 μMChembridge (Fragment library)29030870.137255copper-dependent oxidative stress
0.0463.93E-4SGTC_22597964009 200.0 μMChembridge (Fragment library)3131340.16superoxide
0.0454.78E-4SGTC_13181280-3875 124.0 μMChemDiv (Drug-like library)3732450.216216
0.0446.63E-4SGTC_21715739187 198.1 μMChembridge (Fragment library)22627610.0909091copper-dependent oxidative stress
0.0438.92E-4SGTC_21605660466 132.9 μMChembridge (Fragment library)28591340.086956560S ribosome export
0.0439.46E-4SGTC_29167875993 68.8 μMChembridge (Drug-like library)12992500.0882353
0.0439.94E-4SGTC_28839059145 45.5 μMChembridge (Drug-like library)164765100.0793651
0.0439.93E-4SGTC_7543474-0146 171.0 μMChemDiv (Drug-like library)247620780.060241copper-dependent oxidative stress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2435hr heat shock (37°C) + splitomicin1 μM15269Miscellaneous198.21732.83102heat shock/prefoldin
SGTC_1859st05599820 μM0.361702676301TimTec (Natural product derivative library)288.296783.80213
SGTC_1842st05536261.2 μM0.34676032TimTec (Natural product derivative library)304.296183.5624
SGTC_7510833-0568350 μM0.3035716954252ChemDiv (Drug-like library)311.358322.70924
SGTC_1982st07246771.9 μM0.33163533TimTec (Natural product derivative library)278.32513.99803
SGTC_2898905627058.44 μM0.319154699Chembridge (Drug-like library)352.38381.80215
SGTC_1070358-00222.69 μM0.282051521106ChemDiv (Drug-like library)350.453965.87124SWF1 & branched chain AA biosynthesis
SGTC_1693xanthone101.9 μM0.2777787020TimTec (Natural product derivative library)196.201422.96402DNA damage response
SGTC_2532xanthone78.9 μM0.2777787020Microsource (Natural product library)196.201422.96402
SGTC_1753793-312023.7 μM0.2727275322297ChemDiv (Drug-like library)356.294754.70516
SGTC_20935331342194.4 μM0.27083333839Chembridge (Fragment library)243.300982.25713