3825-7017

N-(4-fluorophenyl)-2-(3-methylphenyl)quinazolin-4-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_425
Screen concentration 13.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 3397440
SMILES CC1=CC=CC(=C1)C2=NC3=CC=CC=C3C(=N2)NC4=CC=C(C=C4)F
Standardized SMILES Cc1cccc(c1)c2nc(Nc3ccc(F)cc3)c4ccccc4n2
Molecular weight 329.3702
ALogP 5.79
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.68
% growth inhibition (Hom. pool) 10


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3397440
Download HIP data (tab-delimited text)  (excel)
Gene:ADE13(YLR359W)|FD-Score:6.62|P-value:1.80E-11|Clearance:1.24||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:CDC13(YDL220C)|FD-Score:-3.35|P-value:3.98E-4|Clearance:0||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:COG3(YER157W)|FD-Score:3.6|P-value:1.60E-4|Clearance:0.01||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DCP1(YOL149W)|FD-Score:3.13|P-value:8.86E-4|Clearance:0.04||SGD DESC:Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress Gene:DED81(YHR019C)|FD-Score:4.25|P-value:1.05E-5|Clearance:0.02||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:DHR2(YKL078W)|FD-Score:8.98|P-value:1.31E-19|Clearance:2.36||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:GPI12(YMR281W)|FD-Score:3.42|P-value:3.10E-4|Clearance:0.01||SGD DESC:ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp Gene:HIP1(YGR191W)|FD-Score:-3.16|P-value:8.01E-4|Clearance:0||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:KOG1(YHR186C)|FD-Score:3.76|P-value:8.46E-5|Clearance:0.03||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:LSG1(YGL099W)|FD-Score:13|P-value:6.92E-39|Clearance:4.01||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MDN1(YLR106C)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.02||SGD DESC:Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus Gene:NAB2(YGL122C)|FD-Score:4.23|P-value:1.14E-5|Clearance:0.36||SGD DESC:Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress Gene:NAB3(YPL190C)|FD-Score:3.83|P-value:6.38E-5|Clearance:0.07||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing Gene:NMD3(YHR170W)|FD-Score:3.87|P-value:5.41E-5|Clearance:0.04||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PAN1(YIR006C)|FD-Score:3.34|P-value:4.25E-4|Clearance:0.02||SGD DESC:Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease Gene:POP7(YBR167C)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.11||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRE1(YER012W)|FD-Score:-3.13|P-value:8.63E-4|Clearance:0||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:QNS1(YHR074W)|FD-Score:3.47|P-value:2.56E-4|Clearance:0.05||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RPS3(YNL178W)|FD-Score:-3.53|P-value:2.09E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP36(YOR287C)|FD-Score:5.38|P-value:3.80E-8|Clearance:0.02||SGD DESC:Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit Gene:RRP46(YGR095C)|FD-Score:-3.14|P-value:8.56E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SEC11(YIR022W)|FD-Score:5.35|P-value:4.28E-8|Clearance:1.1||SGD DESC:18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum Gene:STU2(YLR045C)|FD-Score:3.73|P-value:9.42E-5|Clearance:0.11||SGD DESC:Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p Gene:TFA2(YKR062W)|FD-Score:-3.2|P-value:6.76E-4|Clearance:0||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:UTP4(YDR324C)|FD-Score:-3.86|P-value:5.72E-5|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YJL195C(YJL195C_d)|FD-Score:3.41|P-value:3.22E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YTM1(YOR272W)|FD-Score:-3.74|P-value:9.09E-5|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats Gene:ZPR1(YGR211W)|FD-Score:3.32|P-value:4.58E-4|Clearance:0.19||SGD DESC:Essential protein with two zinc fingers; present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p); relative distribution to the nucleus increases upon DNA replication stress Gene:ADE13(YLR359W)|FD-Score:6.62|P-value:1.80E-11|Clearance:1.24||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:CDC13(YDL220C)|FD-Score:-3.35|P-value:3.98E-4|Clearance:0||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:COG3(YER157W)|FD-Score:3.6|P-value:1.60E-4|Clearance:0.01||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DCP1(YOL149W)|FD-Score:3.13|P-value:8.86E-4|Clearance:0.04||SGD DESC:Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress Gene:DED81(YHR019C)|FD-Score:4.25|P-value:1.05E-5|Clearance:0.02||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:DHR2(YKL078W)|FD-Score:8.98|P-value:1.31E-19|Clearance:2.36||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:GPI12(YMR281W)|FD-Score:3.42|P-value:3.10E-4|Clearance:0.01||SGD DESC:ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp Gene:HIP1(YGR191W)|FD-Score:-3.16|P-value:8.01E-4|Clearance:0||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:KOG1(YHR186C)|FD-Score:3.76|P-value:8.46E-5|Clearance:0.03||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:LSG1(YGL099W)|FD-Score:13|P-value:6.92E-39|Clearance:4.01||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MDN1(YLR106C)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.02||SGD DESC:Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus Gene:NAB2(YGL122C)|FD-Score:4.23|P-value:1.14E-5|Clearance:0.36||SGD DESC:Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress Gene:NAB3(YPL190C)|FD-Score:3.83|P-value:6.38E-5|Clearance:0.07||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing Gene:NMD3(YHR170W)|FD-Score:3.87|P-value:5.41E-5|Clearance:0.04||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PAN1(YIR006C)|FD-Score:3.34|P-value:4.25E-4|Clearance:0.02||SGD DESC:Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease Gene:POP7(YBR167C)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.11||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRE1(YER012W)|FD-Score:-3.13|P-value:8.63E-4|Clearance:0||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:QNS1(YHR074W)|FD-Score:3.47|P-value:2.56E-4|Clearance:0.05||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RPS3(YNL178W)|FD-Score:-3.53|P-value:2.09E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP36(YOR287C)|FD-Score:5.38|P-value:3.80E-8|Clearance:0.02||SGD DESC:Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit Gene:RRP46(YGR095C)|FD-Score:-3.14|P-value:8.56E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SEC11(YIR022W)|FD-Score:5.35|P-value:4.28E-8|Clearance:1.1||SGD DESC:18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum Gene:STU2(YLR045C)|FD-Score:3.73|P-value:9.42E-5|Clearance:0.11||SGD DESC:Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p Gene:TFA2(YKR062W)|FD-Score:-3.2|P-value:6.76E-4|Clearance:0||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:UTP4(YDR324C)|FD-Score:-3.86|P-value:5.72E-5|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YJL195C(YJL195C_d)|FD-Score:3.41|P-value:3.22E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YTM1(YOR272W)|FD-Score:-3.74|P-value:9.09E-5|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats Gene:ZPR1(YGR211W)|FD-Score:3.32|P-value:4.58E-4|Clearance:0.19||SGD DESC:Essential protein with two zinc fingers; present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p); relative distribution to the nucleus increases upon DNA replication stress

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3397440
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AIM9(YER080W)|FD-Score:-6.09|P-value:5.55E-10||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:APM2(YHL019C)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport Gene:ARN2(YHL047C)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C Gene:BCK1(YJL095W)|FD-Score:3.89|P-value:5.09E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BRE1(YDL074C)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BRE5(YNR051C)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:BUD6(YLR319C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate Gene:CKA2(YOR061W)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CKI1(YLR133W)|FD-Score:-5.91|P-value:1.71E-9||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:CLB3(YDL155W)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress Gene:COA1(YIL157C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:COA6(YMR244C-A_p)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Protein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress Gene:COG7(YGL005C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRS5(YOR031W)|FD-Score:3.21|P-value:6.52E-4||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:CSG2(YBR036C)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CTF8(YHR191C)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DAL81(YIR023W)|FD-Score:6.24|P-value:2.17E-10||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DIE2(YGR227W)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:DNF1(YER166W)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:ELM1(YKL048C)|FD-Score:-4.25|P-value:1.06E-5||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:ENV7(YPL236C)|FD-Score:4.46|P-value:4.13E-6||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:FAA3(YIL009W)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:FAR3(YMR052W)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:GAL11(YOL051W)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GEP4(YHR100C)|FD-Score:-5.54|P-value:1.48E-8||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:IES4(YOR189W)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Component of the INO80 chromatiin remodeling complex and target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses Gene:IPK1(YDR315C)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:ISM1(YPL040C)|FD-Score:-3.15|P-value:8.13E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:IVY1(YDR229W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase Gene:KAP123(YER110C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:LNP1(YHR192W_p)|FD-Score:-3.73|P-value:9.59E-5||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MAK3(YPR051W)|FD-Score:5.29|P-value:6.22E-8||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MET28(YIR017C)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism Gene:MPD2(YOL088C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRK1(YDL079C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MRP13(YGR084C)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSS11(YMR164C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:NCS6(YGL211W)|FD-Score:-4.03|P-value:2.74E-5||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NHP10(YDL002C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:OSH2(YDL019C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PBP4(YDL053C)|FD-Score:4.06|P-value:2.40E-5||SGD DESC:Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress Gene:PDR1(YGL013C)|FD-Score:14.9|P-value:2.02E-50||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PES4(YFR023W)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PMP2(YEL017C-A)|FD-Score:5.73|P-value:5.14E-9||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:PSR1(YLL010C)|FD-Score:-5.26|P-value:7.10E-8||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:RBD2(YPL246C)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p Gene:RHR2(YIL053W)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:SBE22(YHR103W)|FD-Score:-3.09|P-value:9.92E-4||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SFG1(YOR315W)|FD-Score:-4.1|P-value:2.11E-5||SGD DESC:Nuclear protein, putative transcription factor required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate Gene:SPO14(YKR031C)|FD-Score:-3.83|P-value:6.47E-5||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:SPO23(YBR250W)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Protein of unknown function; associates with meiosis-specific protein Spo1p Gene:SSK2(YNR031C)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:SUL2(YLR092W)|FD-Score:6.8|P-value:5.11E-12||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SUM1(YDR310C)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SUR1(YPL057C)|FD-Score:5.39|P-value:3.56E-8||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication Gene:SUR4(YLR372W)|FD-Score:8.5|P-value:9.59E-18||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWC5(YBR231C)|FD-Score:4.87|P-value:5.45E-7||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SYF2(YGR129W)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:SYS1(YJL004C)|FD-Score:3.75|P-value:8.91E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAT1(YBR069C)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA1(YMR291W)|FD-Score:-5.21|P-value:9.29E-8||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TMN3(YER113C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn2p; member of Transmembrane Nine family with 9 transmembrane segments; localizes to Golgi; induced by 8-methoxypsoralen plus UVA irradiation Gene:TRE1(YPL176C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:13.5|P-value:5.13E-42||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP16(YPL072W)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Deubiquitinating enzyme anchored to the outer mitochondrial membrane, probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria Gene:URA2(YJL130C)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:USA1(YML029W)|FD-Score:3.87|P-value:5.46E-5||SGD DESC:Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p Gene:VPS3(YDR495C)|FD-Score:-3.83|P-value:6.52E-5||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:YBR285W(YBR285W_p)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YDR134C(YDR134C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Hypothetical protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication Gene:YER034W(YER034W_p)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YGP1(YNL160W)|FD-Score:-4.01|P-value:3.08E-5||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YGR017W(YGR017W_p)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YHL026C(YHL026C_p)|FD-Score:5.07|P-value:1.97E-7||SGD DESC:Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) Gene:YHR045W(YHR045W_p)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:YHR050W-A(YHR050W-A_p)|FD-Score:-5.02|P-value:2.56E-7||SGD DESC:Protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIL002W-A(YIL002W-A_p)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIP4(YGL198W)|FD-Score:5.26|P-value:7.27E-8||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YIR044C(YIR044C)|FD-Score:-3.14|P-value:8.54E-4||SGD DESC:Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YJL127C-B(YJL127C-B_p)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Putative protein of unknown function; identified based on homology to the filamentous fungus, <i>Ashbya gossypii</i> Gene:YKL091C(YKL091C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study Gene:YLR012C(YLR012C_p)|FD-Score:5.53|P-value:1.63E-8||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YLR126C(YLR126C_p)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YLR225C(YLR225C_p)|FD-Score:4.01|P-value:2.98E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YML007C-A(YML007C-A_p)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR316C-A(YMR316C-A_p)|FD-Score:-3.41|P-value:3.23E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YNL092W(YNL092W_p)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene Gene:YNL122C(YNL122C_p)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YOL014W(YOL014W_p)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Putative protein of unknown function Gene:YOR072W(YOR072W_d)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR376W-A(YOR376W-A_p)|FD-Score:-3.21|P-value:6.56E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPK1(YKL126W)|FD-Score:7.75|P-value:4.54E-15||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL136W(YPL136W_d)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C Gene:YRR1(YOR162C)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:YVC1(YOR087W)|FD-Score:3.82|P-value:6.76E-5||SGD DESC:Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock Gene:ACB1(YGR037C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AIM9(YER080W)|FD-Score:-6.09|P-value:5.55E-10||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:APM2(YHL019C)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport Gene:ARN2(YHL047C)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C Gene:BCK1(YJL095W)|FD-Score:3.89|P-value:5.09E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BRE1(YDL074C)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BRE5(YNR051C)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:BUD6(YLR319C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate Gene:CKA2(YOR061W)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CKI1(YLR133W)|FD-Score:-5.91|P-value:1.71E-9||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:CLB3(YDL155W)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress Gene:COA1(YIL157C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:COA6(YMR244C-A_p)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Protein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress Gene:COG7(YGL005C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRS5(YOR031W)|FD-Score:3.21|P-value:6.52E-4||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:CSG2(YBR036C)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CTF8(YHR191C)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DAL81(YIR023W)|FD-Score:6.24|P-value:2.17E-10||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DIE2(YGR227W)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:DNF1(YER166W)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:ELM1(YKL048C)|FD-Score:-4.25|P-value:1.06E-5||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:ENV7(YPL236C)|FD-Score:4.46|P-value:4.13E-6||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:FAA3(YIL009W)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:FAR3(YMR052W)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:GAL11(YOL051W)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GEP4(YHR100C)|FD-Score:-5.54|P-value:1.48E-8||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:IES4(YOR189W)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Component of the INO80 chromatiin remodeling complex and target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses Gene:IPK1(YDR315C)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:ISM1(YPL040C)|FD-Score:-3.15|P-value:8.13E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:IVY1(YDR229W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase Gene:KAP123(YER110C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:LNP1(YHR192W_p)|FD-Score:-3.73|P-value:9.59E-5||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MAK3(YPR051W)|FD-Score:5.29|P-value:6.22E-8||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MET28(YIR017C)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism Gene:MPD2(YOL088C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRK1(YDL079C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MRP13(YGR084C)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSS11(YMR164C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:NCS6(YGL211W)|FD-Score:-4.03|P-value:2.74E-5||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NHP10(YDL002C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:OSH2(YDL019C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PBP4(YDL053C)|FD-Score:4.06|P-value:2.40E-5||SGD DESC:Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress Gene:PDR1(YGL013C)|FD-Score:14.9|P-value:2.02E-50||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PES4(YFR023W)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PMP2(YEL017C-A)|FD-Score:5.73|P-value:5.14E-9||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:PSR1(YLL010C)|FD-Score:-5.26|P-value:7.10E-8||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:RBD2(YPL246C)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p Gene:RHR2(YIL053W)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:SBE22(YHR103W)|FD-Score:-3.09|P-value:9.92E-4||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SFG1(YOR315W)|FD-Score:-4.1|P-value:2.11E-5||SGD DESC:Nuclear protein, putative transcription factor required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate Gene:SPO14(YKR031C)|FD-Score:-3.83|P-value:6.47E-5||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:SPO23(YBR250W)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Protein of unknown function; associates with meiosis-specific protein Spo1p Gene:SSK2(YNR031C)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:SUL2(YLR092W)|FD-Score:6.8|P-value:5.11E-12||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SUM1(YDR310C)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SUR1(YPL057C)|FD-Score:5.39|P-value:3.56E-8||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication Gene:SUR4(YLR372W)|FD-Score:8.5|P-value:9.59E-18||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWC5(YBR231C)|FD-Score:4.87|P-value:5.45E-7||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SYF2(YGR129W)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:SYS1(YJL004C)|FD-Score:3.75|P-value:8.91E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAT1(YBR069C)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA1(YMR291W)|FD-Score:-5.21|P-value:9.29E-8||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TMN3(YER113C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn2p; member of Transmembrane Nine family with 9 transmembrane segments; localizes to Golgi; induced by 8-methoxypsoralen plus UVA irradiation Gene:TRE1(YPL176C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:13.5|P-value:5.13E-42||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP16(YPL072W)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Deubiquitinating enzyme anchored to the outer mitochondrial membrane, probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria Gene:URA2(YJL130C)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:USA1(YML029W)|FD-Score:3.87|P-value:5.46E-5||SGD DESC:Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p Gene:VPS3(YDR495C)|FD-Score:-3.83|P-value:6.52E-5||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:YBR285W(YBR285W_p)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YDR134C(YDR134C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Hypothetical protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication Gene:YER034W(YER034W_p)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YGP1(YNL160W)|FD-Score:-4.01|P-value:3.08E-5||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YGR017W(YGR017W_p)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YHL026C(YHL026C_p)|FD-Score:5.07|P-value:1.97E-7||SGD DESC:Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) Gene:YHR045W(YHR045W_p)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:YHR050W-A(YHR050W-A_p)|FD-Score:-5.02|P-value:2.56E-7||SGD DESC:Protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIL002W-A(YIL002W-A_p)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIP4(YGL198W)|FD-Score:5.26|P-value:7.27E-8||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YIR044C(YIR044C)|FD-Score:-3.14|P-value:8.54E-4||SGD DESC:Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YJL127C-B(YJL127C-B_p)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Putative protein of unknown function; identified based on homology to the filamentous fungus, <i>Ashbya gossypii</i> Gene:YKL091C(YKL091C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study Gene:YLR012C(YLR012C_p)|FD-Score:5.53|P-value:1.63E-8||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YLR126C(YLR126C_p)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YLR225C(YLR225C_p)|FD-Score:4.01|P-value:2.98E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YML007C-A(YML007C-A_p)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR316C-A(YMR316C-A_p)|FD-Score:-3.41|P-value:3.23E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YNL092W(YNL092W_p)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene Gene:YNL122C(YNL122C_p)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YOL014W(YOL014W_p)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Putative protein of unknown function Gene:YOR072W(YOR072W_d)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR376W-A(YOR376W-A_p)|FD-Score:-3.21|P-value:6.56E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPK1(YKL126W)|FD-Score:7.75|P-value:4.54E-15||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL136W(YPL136W_d)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C Gene:YRR1(YOR162C)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:YVC1(YOR087W)|FD-Score:3.82|P-value:6.76E-5||SGD DESC:Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W13.006.92E-394.01LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YKL078W8.981.31E-192.36DHR2Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis
YLR359W6.621.80E-111.24ADE13Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency
YOR287C5.383.80E-80.02RRP36Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit
YIR022W5.354.28E-81.10SEC1118kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum
YHR019C4.251.05E-50.02DED81Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA
YGL122C4.231.14E-50.36NAB2Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress
YHR170W3.875.41E-50.04NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YPL190C3.836.38E-50.07NAB3RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing
YHR186C3.768.46E-50.03KOG1Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
YLR045C3.739.42E-50.11STU2Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p
YLR106C3.621.47E-40.02MDN1Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus
YER157W3.601.60E-40.01COG3Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YBR167C3.591.68E-40.11POP7Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YHR074W3.472.56E-40.05QNS1Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL013C14.902.02E-50PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YBR058C-A13.505.13E-42TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YLR372W8.509.59E-18SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YKL126W7.754.54E-15YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YLR092W6.805.11E-12SUL2High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates
YIR023W6.242.17E-10DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YEL017C-A5.735.14E-9PMP2Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication
YLR012C_p5.531.63E-8YLR012C_pPutative protein of unknown function; YLR012C is not an essential gene
YPL057C5.393.56E-8SUR1Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication
YPR051W5.296.22E-8MAK3Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus
YGL198W5.267.27E-8YIP4Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport
YHL026C_p5.071.97E-7YHL026C_pPutative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History)
YBR231C4.875.45E-7SWC5Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress
YPL236C4.464.13E-6ENV7Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16)
YBR069C4.111.94E-5TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_425
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3127.69E-134SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.0978261PDR1
0.2933.48E-117SGTC_2740felodipine 17.1 μMMiscellaneous33330.0909091
0.2841.29E-109SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.0652174PDR1
0.2761.48E-103SGTC_487nimodipine 119.0 μMMiscellaneous44970.0930233
0.2547.61E-88SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.213115TSC3-RPN4
0.2462.19E-82SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.0869565TSC3-RPN4
0.2341.95E-74SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.138462TSC3-RPN4
0.2245.11E-68SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0285714TSC3-RPN4
0.2194.90E-65SGTC_30259087895 49.5 μMChembridge (Drug-like library)171736410.125PDR1
0.2151.78E-62SGTC_2627acetyl isogambogic acid 100.0 μMMicrosource (Natural product library)68577890.025TSC3-RPN4
0.2138.70E-62SGTC_5481436-0017 110.0 μMChemDiv (Drug-like library)96771370.0987654TSC3-RPN4
0.2093.16E-59SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0649351TSC3-RPN4
0.2086.88E-59SGTC_20925303694 80.7 μMChembridge (Fragment library)19636990.0746269TSC3-RPN4
0.2024.93E-55SGTC_14033909-7815 62.9 μMChemDiv (Drug-like library)66154830.466667
0.2002.16E-54SGTC_2416tofa 880.0 nMMiscellaneous1151750.0571429sphingolipid biosynthesis & PDR1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_14043909-782048.3 μM0.58490623741373ChemDiv (Drug-like library)371.431745.551560S ribosome export
SGTC_14033909-781562.9 μM0.4666676615483ChemDiv (Drug-like library)389.4222035.11216
SGTC_8103825-7150143 μM0.4522330411ChemDiv (Drug-like library)415.48436.021660S ribosome export
SGTC_730327-032516.23 μM0.4489853298ChemDiv (Drug-like library)284.354445.38912DNA damage response
SGTC_14053909-789412 μM0.4035097104060ChemDiv (Drug-like library)293.362963.7111460S ribosome export
SGTC_14063909-790611.9 μM0.38983X1406ChemDiv (Drug-like library)307.389543.7734160S ribosome export
SGTC_3287911717337.06 μM0.3859652789709Chembridge (Drug-like library)329.82393.6692460S ribosome export
SGTC_1983454-287316.19 μM0.381818710957ChemDiv (Drug-like library)297.782044.7461360S ribosome export
SGTC_13261431-209410.8 μM0.372549616278ChemDiv (Drug-like library)234.295764.59112tubulin folding & SWR complex
SGTC_14073909-790912.3 μM0.3593754411780ChemDiv (Drug-like library)319.400244.2011460S ribosome export