1354-0053

4-tert-butyl-6-[[3-[2-(3-chlorophenyl)hydrazinyl]-6-oxocyclohexa-2,4-dien-1-ylidene]methylimino]cyclohexa-2,4-dien-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_427
Screen concentration 47.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 6813657
SMILES CC(C)(C)C1=CC(=NC=C2C=C(C=CC2=O)NNC3=CC(=CC=C3)Cl)C(=O)C=C1
Standardized SMILES CC(C)(C)c1ccc(O)c(c1)N=Cc2cc(ccc2O)N=Nc3cccc(Cl)c3
Molecular weight 407.8927
ALogP 4.2
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0.07
% growth inhibition (Hom. pool) -1.28


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6813657
Download HIP data (tab-delimited text)  (excel)
Gene:CDC28(YBR160W)|FD-Score:3.78|P-value:7.82E-5|Clearance:0.09||SGD DESC:Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress Gene:DOP1(YDR141C)|FD-Score:4.01|P-value:2.98E-5|Clearance:0.23||SGD DESC:Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies Gene:ERD2(YBL040C)|FD-Score:-3.71|P-value:1.04E-4|Clearance:0||SGD DESC:HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins Gene:ERG8(YMR220W)|FD-Score:3.12|P-value:8.99E-4|Clearance:0.13||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GPI19(YDR437W)|FD-Score:-3.29|P-value:5.01E-4|Clearance:0||SGD DESC:Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P Gene:POP1(YNL221C)|FD-Score:3.69|P-value:1.10E-4|Clearance:0.3||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP19(YLL036C)|FD-Score:3.3|P-value:4.83E-4|Clearance:0.18||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:QNS1(YHR074W)|FD-Score:3.38|P-value:3.59E-4|Clearance:0.01||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RPN9(YDR427W)|FD-Score:-3.11|P-value:9.38E-4|Clearance:0||SGD DESC:Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress Gene:SEC21(YNL287W)|FD-Score:3.37|P-value:3.78E-4|Clearance:0.07||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEN15(YMR059W)|FD-Score:-3.49|P-value:2.46E-4|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SHR3(YDL212W)|FD-Score:3.4|P-value:3.42E-4|Clearance:0.01||SGD DESC:Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface Gene:YGR114C(YGR114C_d)|FD-Score:4.5|P-value:3.44E-6|Clearance:0.48||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:CDC28(YBR160W)|FD-Score:3.78|P-value:7.82E-5|Clearance:0.09||SGD DESC:Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress Gene:DOP1(YDR141C)|FD-Score:4.01|P-value:2.98E-5|Clearance:0.23||SGD DESC:Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies Gene:ERD2(YBL040C)|FD-Score:-3.71|P-value:1.04E-4|Clearance:0||SGD DESC:HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins Gene:ERG8(YMR220W)|FD-Score:3.12|P-value:8.99E-4|Clearance:0.13||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GPI19(YDR437W)|FD-Score:-3.29|P-value:5.01E-4|Clearance:0||SGD DESC:Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P Gene:POP1(YNL221C)|FD-Score:3.69|P-value:1.10E-4|Clearance:0.3||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP19(YLL036C)|FD-Score:3.3|P-value:4.83E-4|Clearance:0.18||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:QNS1(YHR074W)|FD-Score:3.38|P-value:3.59E-4|Clearance:0.01||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RPN9(YDR427W)|FD-Score:-3.11|P-value:9.38E-4|Clearance:0||SGD DESC:Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress Gene:SEC21(YNL287W)|FD-Score:3.37|P-value:3.78E-4|Clearance:0.07||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEN15(YMR059W)|FD-Score:-3.49|P-value:2.46E-4|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SHR3(YDL212W)|FD-Score:3.4|P-value:3.42E-4|Clearance:0.01||SGD DESC:Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface Gene:YGR114C(YGR114C_d)|FD-Score:4.5|P-value:3.44E-6|Clearance:0.48||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6813657
Download HOP data (tab-delimited text)  (excel)
Gene:AAD3(YCR107W)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:ABZ2(YMR289W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ACN9(YDR511W)|FD-Score:4.97|P-value:3.39E-7||SGD DESC:Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes Gene:ADR1(YDR216W)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:AEP1(YMR064W)|FD-Score:4.29|P-value:9.02E-6||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AIM44(YPL158C)|FD-Score:-4.16|P-value:1.59E-5||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:APE2(YKL157W)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication Gene:ASR1(YPR093C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein Gene:ATP20(YPR020W)|FD-Score:4.83|P-value:6.80E-7||SGD DESC:Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex Gene:ATP3(YBR039W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:CBT1(YKL208W)|FD-Score:3.79|P-value:7.62E-5||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:COA1(YIL157C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:COX18(YGR062C)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC Gene:DIG2(YDR480W)|FD-Score:6.41|P-value:7.46E-11||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DUS4(YLR405W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:FRE1(YLR214W)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels Gene:FUN14(YAL008W)|FD-Score:4.28|P-value:9.35E-6||SGD DESC:Mitochondrial protein of unknown function Gene:FUN30(YAL019W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate Gene:GBP2(YCL011C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GET1(YGL020C)|FD-Score:-3.31|P-value:4.65E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GIR2(YDR152W)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:HNT2(YDR305C)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins Gene:HRD3(YLR207W)|FD-Score:3.26|P-value:5.47E-4||SGD DESC:Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events Gene:HUA1(YGR268C)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IGD1(YFR017C)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication Gene:IRC24(YIR036C_p)|FD-Score:3.91|P-value:4.59E-5||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:IRC7(YFR055W_p)|FD-Score:-5.47|P-value:2.30E-8||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:ISM1(YPL040C)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:MHF2(YDL160C-A)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 Gene:MNL2(YLR057W)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MOS1(YCL057C-A)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MTG1(YMR097C)|FD-Score:-4.25|P-value:1.05E-5||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:MXR2(YCL033C)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan Gene:NAT5(YOR253W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NUM1(YDR150W)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:PEX27(YOR193W)|FD-Score:4.84|P-value:6.45E-7||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:PGD1(YGL025C)|FD-Score:-4.74|P-value:1.08E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PMP2(YEL017C-A)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:POM34(YLR018C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication Gene:PSD2(YGR170W)|FD-Score:-5.63|P-value:9.05E-9||SGD DESC:Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine Gene:RAD50(YNL250W)|FD-Score:-4.09|P-value:2.17E-5||SGD DESC:Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress Gene:REH1(YLR387C)|FD-Score:-3.34|P-value:4.13E-4||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RPL37B(YDR500C)|FD-Score:-3.19|P-value:6.99E-4||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RTK1(YDL025C)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SBE22(YHR103W)|FD-Score:4.46|P-value:4.08E-6||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SEH1(YGL100W)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SHU1(YHL006C)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SIR1(YKR101W)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin Gene:SIT4(YDL047W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SLO1(YER180C-A)|FD-Score:5.02|P-value:2.62E-7||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:SPO14(YKR031C)|FD-Score:-3.52|P-value:2.20E-4||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:TDH3(YGR192C)|FD-Score:3.88|P-value:5.18E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication Gene:TGL4(YKR089C)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Multifunctional triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p Gene:TRM82(YDR165W)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TSC3(YBR058C-A)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URC2(YDR520C_p)|FD-Score:4.33|P-value:7.48E-6||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:UTP30(YKR060W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:UTR1(YJR049C)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VPS55(YJR044C)|FD-Score:-3.28|P-value:5.28E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YBL008W-A(YBL008W-A_p)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBL070C(YBL070C_d)|FD-Score:6.18|P-value:3.29E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YEL057C(YEL057C_p)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YER130C(YER130C_p)|FD-Score:-6.54|P-value:3.13E-11||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YER175W-A(YER175W-A_p)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGL072C(YGL072C_d)|FD-Score:-3.43|P-value:3.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL149W(YGL149W_d)|FD-Score:3.81|P-value:6.86E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YHL026C(YHL026C_p)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) Gene:YIL001W(YIL001W_p)|FD-Score:4.84|P-value:6.64E-7||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YIL032C(YIL032C_d)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL100W(YIL100W_d)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A Gene:YIL152W(YIL152W_p)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Putative protein of unknown function Gene:YIR018C-A(YIR018C-A_p)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL160C(YJL160C_p)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YLR287C(YLR287C_p)|FD-Score:-5.37|P-value:4.01E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YML002W(YML002W_p)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YMR013W-A(YMR013W-A_d)|FD-Score:-3.73|P-value:9.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73 Gene:YNL140C(YNL140C_p)|FD-Score:5.98|P-value:1.15E-9||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YOR015W(YOR015W_d)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR238W(YOR238W_p)|FD-Score:-3.34|P-value:4.26E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPL191C(YPL191C_p)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:AAD3(YCR107W)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:ABZ2(YMR289W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ACN9(YDR511W)|FD-Score:4.97|P-value:3.39E-7||SGD DESC:Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes Gene:ADR1(YDR216W)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:AEP1(YMR064W)|FD-Score:4.29|P-value:9.02E-6||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AIM44(YPL158C)|FD-Score:-4.16|P-value:1.59E-5||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:APE2(YKL157W)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication Gene:ASR1(YPR093C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein Gene:ATP20(YPR020W)|FD-Score:4.83|P-value:6.80E-7||SGD DESC:Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex Gene:ATP3(YBR039W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:CBT1(YKL208W)|FD-Score:3.79|P-value:7.62E-5||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:COA1(YIL157C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:COX18(YGR062C)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC Gene:DIG2(YDR480W)|FD-Score:6.41|P-value:7.46E-11||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DUS4(YLR405W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:FRE1(YLR214W)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels Gene:FUN14(YAL008W)|FD-Score:4.28|P-value:9.35E-6||SGD DESC:Mitochondrial protein of unknown function Gene:FUN30(YAL019W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate Gene:GBP2(YCL011C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GET1(YGL020C)|FD-Score:-3.31|P-value:4.65E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GIR2(YDR152W)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:HNT2(YDR305C)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins Gene:HRD3(YLR207W)|FD-Score:3.26|P-value:5.47E-4||SGD DESC:Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events Gene:HUA1(YGR268C)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IGD1(YFR017C)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication Gene:IRC24(YIR036C_p)|FD-Score:3.91|P-value:4.59E-5||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:IRC7(YFR055W_p)|FD-Score:-5.47|P-value:2.30E-8||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:ISM1(YPL040C)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:MHF2(YDL160C-A)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 Gene:MNL2(YLR057W)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MOS1(YCL057C-A)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MTG1(YMR097C)|FD-Score:-4.25|P-value:1.05E-5||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:MXR2(YCL033C)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan Gene:NAT5(YOR253W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NUM1(YDR150W)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:PEX27(YOR193W)|FD-Score:4.84|P-value:6.45E-7||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:PGD1(YGL025C)|FD-Score:-4.74|P-value:1.08E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PMP2(YEL017C-A)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:POM34(YLR018C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication Gene:PSD2(YGR170W)|FD-Score:-5.63|P-value:9.05E-9||SGD DESC:Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine Gene:RAD50(YNL250W)|FD-Score:-4.09|P-value:2.17E-5||SGD DESC:Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress Gene:REH1(YLR387C)|FD-Score:-3.34|P-value:4.13E-4||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RPL37B(YDR500C)|FD-Score:-3.19|P-value:6.99E-4||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RTK1(YDL025C)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SBE22(YHR103W)|FD-Score:4.46|P-value:4.08E-6||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SEH1(YGL100W)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SHU1(YHL006C)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SIR1(YKR101W)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin Gene:SIT4(YDL047W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SLO1(YER180C-A)|FD-Score:5.02|P-value:2.62E-7||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:SPO14(YKR031C)|FD-Score:-3.52|P-value:2.20E-4||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:TDH3(YGR192C)|FD-Score:3.88|P-value:5.18E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication Gene:TGL4(YKR089C)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Multifunctional triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p Gene:TRM82(YDR165W)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TSC3(YBR058C-A)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URC2(YDR520C_p)|FD-Score:4.33|P-value:7.48E-6||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:UTP30(YKR060W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:UTR1(YJR049C)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VPS55(YJR044C)|FD-Score:-3.28|P-value:5.28E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YBL008W-A(YBL008W-A_p)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBL070C(YBL070C_d)|FD-Score:6.18|P-value:3.29E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YEL057C(YEL057C_p)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YER130C(YER130C_p)|FD-Score:-6.54|P-value:3.13E-11||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YER175W-A(YER175W-A_p)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGL072C(YGL072C_d)|FD-Score:-3.43|P-value:3.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL149W(YGL149W_d)|FD-Score:3.81|P-value:6.86E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YHL026C(YHL026C_p)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) Gene:YIL001W(YIL001W_p)|FD-Score:4.84|P-value:6.64E-7||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YIL032C(YIL032C_d)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL100W(YIL100W_d)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A Gene:YIL152W(YIL152W_p)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Putative protein of unknown function Gene:YIR018C-A(YIR018C-A_p)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL160C(YJL160C_p)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YLR287C(YLR287C_p)|FD-Score:-5.37|P-value:4.01E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YML002W(YML002W_p)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YMR013W-A(YMR013W-A_d)|FD-Score:-3.73|P-value:9.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73 Gene:YNL140C(YNL140C_p)|FD-Score:5.98|P-value:1.15E-9||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YOR015W(YOR015W_d)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR238W(YOR238W_p)|FD-Score:-3.34|P-value:4.26E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPL191C(YPL191C_p)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR114C_d4.503.44E-60.48YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YDR141C4.012.98E-50.23DOP1Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies
YBR160W3.787.82E-50.09CDC28Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress
YNL221C3.691.10E-40.30POP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YDL212W3.403.42E-40.01SHR3Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface
YHR074W3.383.59E-40.01QNS1Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide
YNL287W3.373.78E-40.07SEC21Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
YLL036C3.304.83E-40.18PRP19Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain
YMR220W3.128.99E-40.13ERG8Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YDR464W2.990.001400.01SPP41Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3
YNL112W2.980.001460.09DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YKL035W2.890.001930.01UGP1UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication
YER159C2.880.002000.08BUR6Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha
YOR160W2.790.002600.06MTR10Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YAR019C2.730.003160.06CDC15Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR480W6.417.46E-11DIG2MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p
YBL070C_d6.183.29E-10YBL070C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YNL140C_p5.981.15E-9YNL140C_pProtein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C
YER180C-A5.022.62E-7SLO1Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO
YDR511W4.973.39E-7ACN9Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes
YOR193W4.846.45E-7PEX27Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p
YIL001W_p4.846.64E-7YIL001W_pPutative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm
YPR020W4.836.80E-7ATP20Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex
YHR103W4.464.08E-6SBE22Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth
YDR520C_p4.337.48E-6URC2_pPutative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism
YMR064W4.299.02E-6AEP1Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase
YAL008W4.289.35E-6FUN14Mitochondrial protein of unknown function
YIL152W_p4.251.08E-5YIL152W_pPutative protein of unknown function
YEL017C-A4.221.22E-5PMP2Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication
YLR057W4.211.26E-5MNL2Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene

GO enrichment analysis for SGTC_427
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0782.39E-9SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0897436TSC3-RPN4
0.0722.91E-8SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.108434TSC3-RPN4
0.0681.83E-7SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.084507TSC3-RPN4
0.0631.50E-6SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0273973TSC3-RPN4
0.0612.55E-6SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0779221TSC3-RPN4
0.0587.03E-6SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.149254TSC3-RPN4
0.0552.30E-5SGTC_2395hr heat shock (37°C) + mitomycin C 819.4 μMMiscellaneous57460.0215054heat shock/prefoldin
0.0552.72E-5SGTC_29529073731 13.8 μMChembridge (Drug-like library)164573530.0875tubulin folding & SWR complex
0.0542.79E-5SGTC_20925303694 80.7 μMChembridge (Fragment library)19636990.056338TSC3-RPN4
0.0543.00E-5SGTC_1815st051655 58.2 μMTimTec (Natural product derivative library)59841560.121951TSC3-RPN4
0.0543.03E-5SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.136986TSC3-RPN4
0.0543.31E-5SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0869565TSC3-RPN4
0.0534.91E-5SGTC_2350330-0087 5.0 mMChemDiv (Drug-like library)464948180.105263TSC3-RPN4
0.0526.66E-5SGTC_1813st051246 8.6 μMTimTec (Natural product derivative library)52919590.0714286TSC3-RPN4
0.0501.04E-4SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0810811TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_12951013-00639.78 μM0.4107146826038ChemDiv (Drug-like library)294.228673.10117
SGTC_8550428-002025 μM0.3846156812414ChemDiv (Drug-like library)226.23072.15914Golgi
SGTC_5720960-008717.7 μM0.3783786796365ChemDiv (Drug-like library)444.869784.8426
SGTC_710417-175047.81 μM0.3582096811472ChemDiv (Drug-like library)364.848024.47714Golgi
SGTC_310508-197457.24 μM0.3484856810525ChemDiv (Drug-like library)414.679724.6213
SGTC_8940960-023855.9 μM0.3478266741907ChemDiv (Drug-like library)340.760422.64724
SGTC_8690564-008044.1 μM0.3392866843321ChemDiv (Drug-like library)317.34133.93125
SGTC_13301469-005461.8 μM0.3378387102664ChemDiv (Drug-like library)382.80372.93536iron homeostasis
SGTC_6961560-0013611 μM0.3114751650269ChemDiv (Drug-like library)327.4356436.1913
SGTC_2881315-001935.1 μM0.3015876811474ChemDiv (Drug-like library)541.120963.9423RSC & ERG11