NS 1619

3-[2-hydroxy-5-(trifluoromethyl)phenyl]-6-(trifluoromethyl)-1H-benzimidazol-2-one

A K+/calcium channel activator.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_432
Screen concentration 61.3 μM
Source ICCB bioactive library
PubChem CID 4552
SMILES C1=CC2=C(C=C1C(F)(F)F)NC(=O)N2C3=C(C=CC(=C3)C(F)(F)F)O
Standardized SMILES Oc1ccc(cc1n2c(O)nc3cc(ccc23)C(F)(F)F)C(F)(F)F
Molecular weight 362.2266
ALogP 4.12
H-bond donor count 2
H-bond acceptor count 8
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.09
% growth inhibition (Hom. pool) 5.11


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4552
Download HIP data (tab-delimited text)  (excel)
Gene:BFR2(YDR299W)|FD-Score:-3.45|P-value:2.83E-4|Clearance:0||SGD DESC:Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock Gene:DUO1(YGL061C)|FD-Score:-4.01|P-value:3.01E-5|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ERG8(YMR220W)|FD-Score:5.24|P-value:8.09E-8|Clearance:0.44||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GLN1(YPR035W)|FD-Score:-3.62|P-value:1.49E-4|Clearance:0||SGD DESC:Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GPI17(YDR434W)|FD-Score:-3.12|P-value:9.16E-4|Clearance:0||SGD DESC:Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog Gene:GRS1(YBR121C)|FD-Score:-3.24|P-value:6.03E-4|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:HEM4(YOR278W)|FD-Score:-3.45|P-value:2.78E-4|Clearance:0||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:MTR3(YGR158C)|FD-Score:3.24|P-value:6.07E-4|Clearance:0.02||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NOC2(YOR206W)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.02||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOP56(YLR197W)|FD-Score:5.46|P-value:2.39E-8|Clearance:0.44||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NOP8(YOL144W)|FD-Score:3.55|P-value:1.93E-4|Clearance:0.31||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUP85(YJR042W)|FD-Score:3.64|P-value:1.36E-4|Clearance:0.07||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:RNT1(YMR239C)|FD-Score:-3.44|P-value:2.96E-4|Clearance:0||SGD DESC:Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes Gene:RPB10(YOR210W)|FD-Score:3.98|P-value:3.39E-5|Clearance:0.34||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPC53(YDL150W)|FD-Score:5.3|P-value:5.81E-8|Clearance:0.44||SGD DESC:RNA polymerase III subunit C53 Gene:RPS15(YOL040C)|FD-Score:4.33|P-value:7.42E-6|Clearance:0.07||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RRP9(YPR137W)|FD-Score:3.12|P-value:9.10E-4|Clearance:0.03||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RSC8(YFR037C)|FD-Score:4.8|P-value:7.99E-7|Clearance:0.22||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC6(YIL068C)|FD-Score:4.12|P-value:1.93E-5|Clearance:0.1||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:SLD3(YGL113W)|FD-Score:4.26|P-value:1.01E-5|Clearance:0.15||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SUI2(YJR007W)|FD-Score:4.01|P-value:2.99E-5|Clearance:0.03||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TAD2(YJL035C)|FD-Score:-4.13|P-value:1.84E-5|Clearance:0||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs Gene:TAF5(YBR198C)|FD-Score:4.58|P-value:2.37E-6|Clearance:0.24||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:VRG4(YGL225W)|FD-Score:-3.35|P-value:3.99E-4|Clearance:0||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:YAL034C-B(YAL034C-B_d)|FD-Score:-3.56|P-value:1.87E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL044C(YPL044C_d)|FD-Score:3.22|P-value:6.49E-4|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W Gene:YRB1(YDR002W)|FD-Score:-3.28|P-value:5.24E-4|Clearance:0||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1 Gene:BFR2(YDR299W)|FD-Score:-3.45|P-value:2.83E-4|Clearance:0||SGD DESC:Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock Gene:DUO1(YGL061C)|FD-Score:-4.01|P-value:3.01E-5|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ERG8(YMR220W)|FD-Score:5.24|P-value:8.09E-8|Clearance:0.44||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GLN1(YPR035W)|FD-Score:-3.62|P-value:1.49E-4|Clearance:0||SGD DESC:Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GPI17(YDR434W)|FD-Score:-3.12|P-value:9.16E-4|Clearance:0||SGD DESC:Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog Gene:GRS1(YBR121C)|FD-Score:-3.24|P-value:6.03E-4|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:HEM4(YOR278W)|FD-Score:-3.45|P-value:2.78E-4|Clearance:0||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:MTR3(YGR158C)|FD-Score:3.24|P-value:6.07E-4|Clearance:0.02||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NOC2(YOR206W)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.02||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOP56(YLR197W)|FD-Score:5.46|P-value:2.39E-8|Clearance:0.44||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NOP8(YOL144W)|FD-Score:3.55|P-value:1.93E-4|Clearance:0.31||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUP85(YJR042W)|FD-Score:3.64|P-value:1.36E-4|Clearance:0.07||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:RNT1(YMR239C)|FD-Score:-3.44|P-value:2.96E-4|Clearance:0||SGD DESC:Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes Gene:RPB10(YOR210W)|FD-Score:3.98|P-value:3.39E-5|Clearance:0.34||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPC53(YDL150W)|FD-Score:5.3|P-value:5.81E-8|Clearance:0.44||SGD DESC:RNA polymerase III subunit C53 Gene:RPS15(YOL040C)|FD-Score:4.33|P-value:7.42E-6|Clearance:0.07||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RRP9(YPR137W)|FD-Score:3.12|P-value:9.10E-4|Clearance:0.03||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RSC8(YFR037C)|FD-Score:4.8|P-value:7.99E-7|Clearance:0.22||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC6(YIL068C)|FD-Score:4.12|P-value:1.93E-5|Clearance:0.1||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:SLD3(YGL113W)|FD-Score:4.26|P-value:1.01E-5|Clearance:0.15||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SUI2(YJR007W)|FD-Score:4.01|P-value:2.99E-5|Clearance:0.03||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TAD2(YJL035C)|FD-Score:-4.13|P-value:1.84E-5|Clearance:0||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs Gene:TAF5(YBR198C)|FD-Score:4.58|P-value:2.37E-6|Clearance:0.24||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:VRG4(YGL225W)|FD-Score:-3.35|P-value:3.99E-4|Clearance:0||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:YAL034C-B(YAL034C-B_d)|FD-Score:-3.56|P-value:1.87E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL044C(YPL044C_d)|FD-Score:3.22|P-value:6.49E-4|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W Gene:YRB1(YDR002W)|FD-Score:-3.28|P-value:5.24E-4|Clearance:0||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4552
Download HOP data (tab-delimited text)  (excel)
Gene:AEP3(YPL005W)|FD-Score:5.25|P-value:7.54E-8||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:ARG81(YML099C)|FD-Score:3.74|P-value:9.09E-5||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ATG32(YIL146C)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane Gene:ATP19(YOL077W-A)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase Gene:AVO2(YMR068W)|FD-Score:-3.29|P-value:4.98E-4||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:BRP1(YGL007W_d)|FD-Score:4.58|P-value:2.35E-6||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BUD31(YCR063W)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:CDC50(YCR094W)|FD-Score:6.46|P-value:5.24E-11||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CHS3(YBR023C)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CLA4(YNL298W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG5(YNL051W)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRN1(YLR429W)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly Gene:CUE1(YMR264W)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DAL7(YIR031C)|FD-Score:5.18|P-value:1.14E-7||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DCS1(YLR270W)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DUF1(YOL087C)|FD-Score:-4.76|P-value:9.82E-7||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ERG2(YMR202W)|FD-Score:3.84|P-value:6.23E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG28(YER044C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p Gene:FLC3(YGL139W)|FD-Score:4.29|P-value:9.02E-6||SGD DESC:Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER Gene:GAL4(YPL248C)|FD-Score:-3.39|P-value:3.53E-4||SGD DESC:DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p Gene:GPT2(YKR067W)|FD-Score:5.19|P-value:1.04E-7||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:HSE1(YHL002W)|FD-Score:4.76|P-value:9.88E-7||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:IKI1(YHR187W)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:IMD3(YLR432W)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:INM2(YDR287W)|FD-Score:4.71|P-value:1.22E-6||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy Gene:KAR9(YPL269W)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:LOT5(YKL183W)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress Gene:LYS2(YBR115C)|FD-Score:-5.04|P-value:2.33E-7||SGD DESC:Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p Gene:MDM20(YOL076W)|FD-Score:-3.28|P-value:5.28E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MET22(YOL064C)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MGR3(YMR115W)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MPC54(YOR177C)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MRPL35(YDR322W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL51(YPR100W)|FD-Score:5.9|P-value:1.84E-9||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRS4(YKR052C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication Gene:MTH1(YDR277C)|FD-Score:-4.23|P-value:1.17E-5||SGD DESC:Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation Gene:NQM1(YGR043C)|FD-Score:-3.15|P-value:8.04E-4||SGD DESC:Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift Gene:PAA1(YDR071C)|FD-Score:3.77|P-value:8.30E-5||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PAR32(YDL173W)|FD-Score:4.9|P-value:4.73E-7||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PBA1(YLR199C)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly Gene:PEX22(YAL055W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:PEX7(YDR142C)|FD-Score:-3.1|P-value:9.80E-4||SGD DESC:Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) Gene:PFK1(YGR240C)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:POR1(YNL055C)|FD-Score:4|P-value:3.21E-5||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PTH2(YBL057C)|FD-Score:4.42|P-value:4.97E-6||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:PYC1(YGL062W)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:RAD33(YML011C)|FD-Score:5.26|P-value:7.09E-8||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RIM4(YHL024W)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RMR1(YGL250W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus Gene:RPL13B(YMR142C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPP1B(YDL130W)|FD-Score:4.42|P-value:5.01E-6||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPS24A(YER074W)|FD-Score:-5.09|P-value:1.81E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RPS30A(YLR287C-A)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RTC3(YHR087W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTC6(YPL183W-A)|FD-Score:4.53|P-value:2.91E-6||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RUP1(YOR138C)|FD-Score:7.35|P-value:1.01E-13||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SCM4(YGR049W)|FD-Score:-4.52|P-value:3.13E-6||SGD DESC:Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation Gene:SER33(YIL074C)|FD-Score:-3.1|P-value:9.81E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SET4(YJL105W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SIW14(YNL032W)|FD-Score:-5.08|P-value:1.88E-7||SGD DESC:Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm Gene:SLG1(YOR008C)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLP1(YOR154W_p)|FD-Score:3.98|P-value:3.43E-5||SGD DESC:Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p Gene:SLX9(YGR081C)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SNC1(YAL030W)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SOM1(YEL059C-A)|FD-Score:4.45|P-value:4.38E-6||SGD DESC:Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs Gene:SPO16(YHR153C)|FD-Score:3.83|P-value:6.47E-5||SGD DESC:Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation Gene:SRL1(YOR247W)|FD-Score:-4.28|P-value:9.31E-6||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:STD1(YOR047C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:STP22(YCL008C)|FD-Score:-3.17|P-value:7.69E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SXM1(YDR395W)|FD-Score:3.73|P-value:9.39E-5||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:TRF5(YNL299W)|FD-Score:3.84|P-value:6.19E-5||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:TSC3(YBR058C-A)|FD-Score:-4.55|P-value:2.71E-6||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UGA4(YDL210W)|FD-Score:-3.32|P-value:4.58E-4||SGD DESC:Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane Gene:ULI1(YFR026C)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Putative protein of unknown function involved in and induced by the endoplasmic reticulum unfolded protein response Gene:UME6(YDR207C)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:VHR2(YER064C_p)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:VPS17(YOR132W)|FD-Score:4.59|P-value:2.18E-6||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:YBL044W(YBL044W_p)|FD-Score:3.87|P-value:5.49E-5||SGD DESC:Putative protein of unknown function; YBL044W is not an essential protein Gene:YBR141C(YBR141C_p)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YBR221W-A(YBR221W-A_p)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDL183C(YDL183C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YER085C(YER085C_p)|FD-Score:4.41|P-value:5.27E-6||SGD DESC:Putative protein of unknown function Gene:YFR039C(YFR039C_p)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces Gene:YGL185C(YGL185C_p)|FD-Score:-3.91|P-value:4.53E-5||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGR111W(YGR111W_p)|FD-Score:-3.9|P-value:4.79E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YHP1(YDR451C)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YLL007C(YLL007C_p)|FD-Score:-3.21|P-value:6.65E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YML003W(YML003W_p)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Putative protein of unknown function Gene:YNL043C(YNL043C_d)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YNL134C(YNL134C_p)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:Protein of unknown function; similar to dehydrogenases from other model organisms; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:YNL303W(YNL303W_d)|FD-Score:5.95|P-value:1.36E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR022C(YOR022C_p)|FD-Score:4.52|P-value:3.07E-6||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR331C(YOR331C_d)|FD-Score:5.56|P-value:1.36E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W Gene:YOR338W(YOR338W_p)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication Gene:YOR364W(YOR364W_d)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Gene:YPL062W(YPL062W_d)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YRM1(YOR172W)|FD-Score:3.99|P-value:3.32E-5||SGD DESC:Zn2-Cys6 zinc-finger transcription factor that activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes Gene:AEP3(YPL005W)|FD-Score:5.25|P-value:7.54E-8||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:ARG81(YML099C)|FD-Score:3.74|P-value:9.09E-5||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ATG32(YIL146C)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane Gene:ATP19(YOL077W-A)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase Gene:AVO2(YMR068W)|FD-Score:-3.29|P-value:4.98E-4||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:BRP1(YGL007W_d)|FD-Score:4.58|P-value:2.35E-6||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BUD31(YCR063W)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:CDC50(YCR094W)|FD-Score:6.46|P-value:5.24E-11||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CHS3(YBR023C)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CLA4(YNL298W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG5(YNL051W)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRN1(YLR429W)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly Gene:CUE1(YMR264W)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DAL7(YIR031C)|FD-Score:5.18|P-value:1.14E-7||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DCS1(YLR270W)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DUF1(YOL087C)|FD-Score:-4.76|P-value:9.82E-7||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ERG2(YMR202W)|FD-Score:3.84|P-value:6.23E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG28(YER044C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p Gene:FLC3(YGL139W)|FD-Score:4.29|P-value:9.02E-6||SGD DESC:Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER Gene:GAL4(YPL248C)|FD-Score:-3.39|P-value:3.53E-4||SGD DESC:DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p Gene:GPT2(YKR067W)|FD-Score:5.19|P-value:1.04E-7||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:HSE1(YHL002W)|FD-Score:4.76|P-value:9.88E-7||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:IKI1(YHR187W)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:IMD3(YLR432W)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:INM2(YDR287W)|FD-Score:4.71|P-value:1.22E-6||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy Gene:KAR9(YPL269W)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:LOT5(YKL183W)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress Gene:LYS2(YBR115C)|FD-Score:-5.04|P-value:2.33E-7||SGD DESC:Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p Gene:MDM20(YOL076W)|FD-Score:-3.28|P-value:5.28E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MET22(YOL064C)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MGR3(YMR115W)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MPC54(YOR177C)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MRPL35(YDR322W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL51(YPR100W)|FD-Score:5.9|P-value:1.84E-9||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRS4(YKR052C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication Gene:MTH1(YDR277C)|FD-Score:-4.23|P-value:1.17E-5||SGD DESC:Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation Gene:NQM1(YGR043C)|FD-Score:-3.15|P-value:8.04E-4||SGD DESC:Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift Gene:PAA1(YDR071C)|FD-Score:3.77|P-value:8.30E-5||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PAR32(YDL173W)|FD-Score:4.9|P-value:4.73E-7||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PBA1(YLR199C)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly Gene:PEX22(YAL055W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:PEX7(YDR142C)|FD-Score:-3.1|P-value:9.80E-4||SGD DESC:Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) Gene:PFK1(YGR240C)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:POR1(YNL055C)|FD-Score:4|P-value:3.21E-5||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PTH2(YBL057C)|FD-Score:4.42|P-value:4.97E-6||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:PYC1(YGL062W)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:RAD33(YML011C)|FD-Score:5.26|P-value:7.09E-8||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RIM4(YHL024W)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RMR1(YGL250W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus Gene:RPL13B(YMR142C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPP1B(YDL130W)|FD-Score:4.42|P-value:5.01E-6||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPS24A(YER074W)|FD-Score:-5.09|P-value:1.81E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RPS30A(YLR287C-A)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RTC3(YHR087W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTC6(YPL183W-A)|FD-Score:4.53|P-value:2.91E-6||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RUP1(YOR138C)|FD-Score:7.35|P-value:1.01E-13||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SCM4(YGR049W)|FD-Score:-4.52|P-value:3.13E-6||SGD DESC:Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation Gene:SER33(YIL074C)|FD-Score:-3.1|P-value:9.81E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SET4(YJL105W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SIW14(YNL032W)|FD-Score:-5.08|P-value:1.88E-7||SGD DESC:Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm Gene:SLG1(YOR008C)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLP1(YOR154W_p)|FD-Score:3.98|P-value:3.43E-5||SGD DESC:Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p Gene:SLX9(YGR081C)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SNC1(YAL030W)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SOM1(YEL059C-A)|FD-Score:4.45|P-value:4.38E-6||SGD DESC:Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs Gene:SPO16(YHR153C)|FD-Score:3.83|P-value:6.47E-5||SGD DESC:Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation Gene:SRL1(YOR247W)|FD-Score:-4.28|P-value:9.31E-6||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:STD1(YOR047C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:STP22(YCL008C)|FD-Score:-3.17|P-value:7.69E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SXM1(YDR395W)|FD-Score:3.73|P-value:9.39E-5||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:TRF5(YNL299W)|FD-Score:3.84|P-value:6.19E-5||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:TSC3(YBR058C-A)|FD-Score:-4.55|P-value:2.71E-6||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UGA4(YDL210W)|FD-Score:-3.32|P-value:4.58E-4||SGD DESC:Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane Gene:ULI1(YFR026C)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Putative protein of unknown function involved in and induced by the endoplasmic reticulum unfolded protein response Gene:UME6(YDR207C)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:VHR2(YER064C_p)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:VPS17(YOR132W)|FD-Score:4.59|P-value:2.18E-6||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:YBL044W(YBL044W_p)|FD-Score:3.87|P-value:5.49E-5||SGD DESC:Putative protein of unknown function; YBL044W is not an essential protein Gene:YBR141C(YBR141C_p)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YBR221W-A(YBR221W-A_p)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDL183C(YDL183C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YER085C(YER085C_p)|FD-Score:4.41|P-value:5.27E-6||SGD DESC:Putative protein of unknown function Gene:YFR039C(YFR039C_p)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces Gene:YGL185C(YGL185C_p)|FD-Score:-3.91|P-value:4.53E-5||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGR111W(YGR111W_p)|FD-Score:-3.9|P-value:4.79E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YHP1(YDR451C)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YLL007C(YLL007C_p)|FD-Score:-3.21|P-value:6.65E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YML003W(YML003W_p)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Putative protein of unknown function Gene:YNL043C(YNL043C_d)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YNL134C(YNL134C_p)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:Protein of unknown function; similar to dehydrogenases from other model organisms; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:YNL303W(YNL303W_d)|FD-Score:5.95|P-value:1.36E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR022C(YOR022C_p)|FD-Score:4.52|P-value:3.07E-6||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR331C(YOR331C_d)|FD-Score:5.56|P-value:1.36E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W Gene:YOR338W(YOR338W_p)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication Gene:YOR364W(YOR364W_d)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Gene:YPL062W(YPL062W_d)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YRM1(YOR172W)|FD-Score:3.99|P-value:3.32E-5||SGD DESC:Zn2-Cys6 zinc-finger transcription factor that activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR197W5.462.39E-80.44NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YDL150W5.305.81E-80.44RPC53RNA polymerase III subunit C53
YMR220W5.248.09E-80.44ERG8Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YFR037C4.807.99E-70.22RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YBR198C4.582.37E-60.24TAF5Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YOL040C4.337.42E-60.07RPS15Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
YGL113W4.261.01E-50.14SLD3Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress
YIL068C4.121.93E-50.10SEC6Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
YJR007W4.012.99E-50.03SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
YOR210W3.983.39E-50.34RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YJR042W3.641.36E-40.07NUP85Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75
YOR206W3.571.81E-40.02NOC2Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
YOL144W3.551.93E-40.32NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YGR158C3.246.07E-40.02MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
YPL044C_d3.226.49E-40.10YPL044C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR138C7.351.01E-13RUP1Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress
YCR094W6.465.24E-11CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YNL303W_d5.951.36E-9YNL303W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YPR100W5.901.84E-9MRPL51Mitochondrial ribosomal protein of the large subunit
YOR331C_d5.561.36E-8YOR331C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W
YML011C5.267.09E-8RAD33Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
YPL005W5.257.54E-8AEP3Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA
YKR067W5.191.04E-7GPT2Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen
YIR031C5.181.14E-7DAL7Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
YKL183W5.151.31E-7LOT5Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress
YDL173W4.904.73E-7PAR32Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene
YHL002W4.769.88E-7HSE1Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes
YDR287W4.711.22E-6INM2Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy
YOR132W4.592.18E-6VPS17Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes
YGL007W_d4.582.35E-6BRP1_dDubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1

GO enrichment analysis for SGTC_432
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0773.34E-9SGTC_440fluspirilene 35.1 μMICCB bioactive library33960.105263
0.0764.43E-9SGTC_8003448-7362 155.0 μMChemDiv (Drug-like library)159919130.0506329translation
0.0672.63E-7SGTC_2652actinonin 100.0 μMMicrosource (Natural product library)4436000.0357143
0.0672.67E-7SGTC_2734clemastine 41.6 μMMiscellaneous269870.1ERG2
0.0631.33E-6SGTC_22107115885 190.4 μMChembridge (Fragment library)29270390.0714286cell wall signaling
0.0612.65E-6SGTC_9282764-0428 58.7 μMChemDiv (Drug-like library)6625780.0136986cell wall
0.0613.02E-6SGTC_1466k213-0079 57.5 μMChemDiv (Drug-like library)36579810.0526316
0.0603.39E-6SGTC_28629027634 71.4 μMChembridge (Drug-like library)50125620.0649351ERG2
0.0604.18E-6SGTC_9083448-7491 47.7 μMChemDiv (Drug-like library)12030310.0833333mitochondrial stress
0.0595.14E-6SGTC_2694st077685 81.9 μMTimTec (Natural product derivative library)28380960.0694444endomembrane recycling
0.0587.50E-6SGTC_31279126946 49.5 μMChembridge (Drug-like library)272432490.0483871ERG2
0.0589.38E-6SGTC_20474141938 200.0 μMChembridge (Fragment library)38078620.0892857
0.0571.35E-5SGTC_8324083-0019 164.0 μMChemDiv (Drug-like library)19907580.111111translation
0.0571.36E-5SGTC_2549cedrol 20.9 μMMicrosource (Natural product library)67086650.0163934
0.0571.36E-5SGTC_32409133527 49.5 μMChembridge (Drug-like library)176174550.0675676

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_12951013-00639.78 μM0.2962966826038ChemDiv (Drug-like library)294.228673.10117
SGTC_21846075246200 μM0.2692312267320Chembridge (Fragment library)260.288442.13513
SGTC_2304744301691.4 μM0.267857939854Chembridge (Fragment library)260.235592.82415
SGTC_2714st07931356.9 μM0.2666672939799TimTec (Natural product derivative library)351.319753.46916
SGTC_5231934-022213.7 μM0.2631581361595ChemDiv (Drug-like library)289.296653.78316
SGTC_1139k015-002446.9 μM0.2631582766652ChemDiv (Drug-like library)305.251313.40205PDR1
SGTC_6383970-08374.09 μM0.2586214118451ChemDiv (Drug-like library)337.293172.19606
SGTC_1093fluphenazine7.97 μM0.256602611NIH Clinical Collection473.982494.81728
SGTC_2729fluphenazine10.66 μM0.253372Miscellaneous437.521554.43518
SGTC_1605st00008249.1 μM0.2424241114022TimTec (Natural product derivative library)407.452513.9270760S ribosome export
SGTC_1606st00009449.2 μM0.2352941242210TimTec (Natural product derivative library)406.464455.2921660S ribosome export