n,n-dimethylsphingosine

(E,2S,3R)-2-(dimethylamino)octadec-4-ene-1,3-diol

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_436
Screen concentration 3.3 μM
Source ICCB bioactive library
PubChem CID 5282309
SMILES CCCCCCCCCCCCCC=CC(C(CO)N(C)C)O
Standardized SMILES CCCCCCCCCCCCCC=CC(O)C(CO)N(C)C
Molecular weight 327.545
ALogP 5.79
H-bond donor count 2
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.39
% growth inhibition (Hom. pool) 3.97


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5282309
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:-6.24|P-value:2.19E-10|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:BRN1(YBL097W)|FD-Score:-3.3|P-value:4.77E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CAK1(YFL029C)|FD-Score:3.3|P-value:4.82E-4|Clearance:0||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CDC42(YLR229C)|FD-Score:3.5|P-value:2.31E-4|Clearance:0.09||SGD DESC:Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins Gene:CWC2(YDL209C)|FD-Score:3.25|P-value:5.82E-4|Clearance:0.19||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:DED81(YHR019C)|FD-Score:9.07|P-value:5.74E-20|Clearance:5.3||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:GPI19(YDR437W)|FD-Score:-4.2|P-value:1.31E-5|Clearance:0||SGD DESC:Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P Gene:LST8(YNL006W)|FD-Score:-3.36|P-value:3.94E-4|Clearance:0||SGD DESC:Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat Gene:MET4(YNL103W)|FD-Score:3.41|P-value:3.28E-4|Clearance:0.11||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:MPS1(YDL028C)|FD-Score:-3.67|P-value:1.20E-4|Clearance:0||SGD DESC:Dual-specificity kinase; required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p Gene:NDD1(YOR372C)|FD-Score:3.3|P-value:4.80E-4|Clearance:0||SGD DESC:Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes Gene:NOP56(YLR197W)|FD-Score:-5.03|P-value:2.50E-7|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NUP145(YGL092W)|FD-Score:-3.2|P-value:6.78E-4|Clearance:0||SGD DESC:Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 Gene:POP5(YAL033W)|FD-Score:3.3|P-value:4.90E-4|Clearance:0.01||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP28(YDR243C)|FD-Score:3.28|P-value:5.15E-4|Clearance:0.03||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:PSF2(YJL072C)|FD-Score:3.77|P-value:8.18E-5|Clearance:0.27||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RPC19(YNL113W)|FD-Score:-3.18|P-value:7.37E-4|Clearance:0||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RTS2(YOR077W)|FD-Score:-3.17|P-value:7.60E-4|Clearance:0||SGD DESC:Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication Gene:SMC4(YLR086W)|FD-Score:-3.43|P-value:3.06E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:TFB1(YDR311W)|FD-Score:-3.73|P-value:9.69E-5|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:ACT1(YFL039C)|FD-Score:-6.24|P-value:2.19E-10|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:BRN1(YBL097W)|FD-Score:-3.3|P-value:4.77E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CAK1(YFL029C)|FD-Score:3.3|P-value:4.82E-4|Clearance:0||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CDC42(YLR229C)|FD-Score:3.5|P-value:2.31E-4|Clearance:0.09||SGD DESC:Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins Gene:CWC2(YDL209C)|FD-Score:3.25|P-value:5.82E-4|Clearance:0.19||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:DED81(YHR019C)|FD-Score:9.07|P-value:5.74E-20|Clearance:5.3||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:GPI19(YDR437W)|FD-Score:-4.2|P-value:1.31E-5|Clearance:0||SGD DESC:Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P Gene:LST8(YNL006W)|FD-Score:-3.36|P-value:3.94E-4|Clearance:0||SGD DESC:Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat Gene:MET4(YNL103W)|FD-Score:3.41|P-value:3.28E-4|Clearance:0.11||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:MPS1(YDL028C)|FD-Score:-3.67|P-value:1.20E-4|Clearance:0||SGD DESC:Dual-specificity kinase; required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p Gene:NDD1(YOR372C)|FD-Score:3.3|P-value:4.80E-4|Clearance:0||SGD DESC:Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes Gene:NOP56(YLR197W)|FD-Score:-5.03|P-value:2.50E-7|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NUP145(YGL092W)|FD-Score:-3.2|P-value:6.78E-4|Clearance:0||SGD DESC:Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 Gene:POP5(YAL033W)|FD-Score:3.3|P-value:4.90E-4|Clearance:0.01||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP28(YDR243C)|FD-Score:3.28|P-value:5.15E-4|Clearance:0.03||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:PSF2(YJL072C)|FD-Score:3.77|P-value:8.18E-5|Clearance:0.27||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RPC19(YNL113W)|FD-Score:-3.18|P-value:7.37E-4|Clearance:0||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RTS2(YOR077W)|FD-Score:-3.17|P-value:7.60E-4|Clearance:0||SGD DESC:Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication Gene:SMC4(YLR086W)|FD-Score:-3.43|P-value:3.06E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:TFB1(YDR311W)|FD-Score:-3.73|P-value:9.69E-5|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5282309
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ADH2(YMR303C)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 Gene:ADY2(YCR010C)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:AEP2(YMR282C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AIR1(YIL079C)|FD-Score:4.91|P-value:4.59E-7||SGD DESC:Zinc knuckle protein; involved in nuclear RNA processing and degradation as a component of the TRAMP complex; stimulates the poly(A) polymerase activity of Pap2p in vitro; AIR1 has a paralog, AIR2, that arose from the whole genome duplication Gene:BUB2(YMR055C)|FD-Score:6.15|P-value:3.92E-10||SGD DESC:Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage Gene:BUD30(YDL151C_d)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CCW12(YLR110C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CMC4(YMR194C-B)|FD-Score:7.08|P-value:7.08E-13||SGD DESC:Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs Gene:COG5(YNL051W)|FD-Score:5.75|P-value:4.57E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DCS1(YLR270W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DJP1(YIR004W)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ Gene:ECM25(YJL201W)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:ECM29(YHL030W)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ERG4(YGL012W)|FD-Score:-3.17|P-value:7.54E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FCY1(YPR062W)|FD-Score:5.63|P-value:9.07E-9||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FDC1(YDR539W)|FD-Score:4.81|P-value:7.39E-7||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:FLC3(YGL139W)|FD-Score:-3.13|P-value:8.85E-4||SGD DESC:Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER Gene:FRK1(YPL141C)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:GAS5(YOL030W)|FD-Score:-5.21|P-value:9.50E-8||SGD DESC:1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall Gene:GEP5(YLR091W)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:HOM6(YJR139C)|FD-Score:4.56|P-value:2.51E-6||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HPF1(YOL155C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:HSP31(YDR533C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:HTA1(YDR225W)|FD-Score:-3.28|P-value:5.26E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:HTZ1(YOL012C)|FD-Score:5.83|P-value:2.83E-9||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:IDH2(YOR136W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:JHD1(YER051W)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe Gene:KIN2(YLR096W)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:MBA1(YBR185C)|FD-Score:5.69|P-value:6.42E-9||SGD DESC:Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane Gene:MDM32(YOR147W)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MDY2(YOL111C)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MEF1(YLR069C)|FD-Score:3.81|P-value:6.84E-5||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MNN11(YJL183W)|FD-Score:3.93|P-value:4.21E-5||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MNS1(YJR131W)|FD-Score:-4|P-value:3.21E-5||SGD DESC:Alpha-1,2-mannosidase involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation Gene:MOS1(YCL057C-A)|FD-Score:5.39|P-value:3.48E-8||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MOT3(YMR070W)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MTH1(YDR277C)|FD-Score:3.77|P-value:8.21E-5||SGD DESC:Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation Gene:MUM3(YOR298W)|FD-Score:4.96|P-value:3.54E-7||SGD DESC:Protein of unknown function involved in the organization of the outer spore wall layers; has similarity to the tafazzins superfamily of acyltransferases Gene:NEW1(YPL226W)|FD-Score:4.31|P-value:8.16E-6||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NIT2(YJL126W)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:OPI3(YJR073C)|FD-Score:-3.11|P-value:9.48E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PAA1(YDR071C)|FD-Score:5.4|P-value:3.38E-8||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PAL1(YDR348C)|FD-Score:-3.31|P-value:4.66E-4||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PAR32(YDL173W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PDR18(YNR070W_p)|FD-Score:-4.08|P-value:2.22E-5||SGD DESC:Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PEX17(YNL214W)|FD-Score:3.88|P-value:5.13E-5||SGD DESC:Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis Gene:PHO81(YGR233C)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PSR2(YLR019W)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Functionally redundant Psr1p homolog, a plasma membrane phosphatase involved in the general stress response; required with Psr1p and Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:RAD27(YKL113C)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RCK1(YGL158W)|FD-Score:-3.12|P-value:8.94E-4||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:RIM101(YHL027W)|FD-Score:3.97|P-value:3.53E-5||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM9(YMR063W)|FD-Score:4.99|P-value:3.03E-7||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPL24A(YGL031C)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RTT106(YNL206C)|FD-Score:-4.25|P-value:1.05E-5||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:SIC1(YLR079W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SLP1(YOR154W_p)|FD-Score:4.47|P-value:3.88E-6||SGD DESC:Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p Gene:SLT2(YHR030C)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SMY2(YBR172C)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Protein of unknown function involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication Gene:SPF1(YEL031W)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SUR1(YPL057C)|FD-Score:-4.02|P-value:2.95E-5||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication Gene:TOM1(YDR457W)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TOM71(YHR117W)|FD-Score:4.87|P-value:5.44E-7||SGD DESC:Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication Gene:TRX3(YCR083W)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p Gene:UGA3(YDL170W)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:UPC2(YDR213W)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication Gene:VMR1(YHL035C)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:YBL100W-C(YBL100W-C_p)|FD-Score:-3.74|P-value:9.17E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCR050C(YCR050C_p)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YEL028W(YEL028W_d)|FD-Score:4.79|P-value:8.16E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR169C-A(YGR169C-A_p)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YHI9(YHR029C)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YIL054W(YIL054W_p)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YIL096C(YIL096C_p)|FD-Score:5.35|P-value:4.35E-8||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis Gene:YKL115C(YKL115C_d)|FD-Score:4.5|P-value:3.35E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YKR051W(YKR051W_p)|FD-Score:-3.15|P-value:8.19E-4||SGD DESC:Putative protein of unknown function Gene:YLR334C(YLR334C_d)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here Gene:YLR361C-A(YLR361C-A_p)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Putative protein of unknown function Gene:YMR034C(YMR034C_p)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YMR135W-A(YMR135W-A_d)|FD-Score:-3.18|P-value:7.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL050C(YNL050C_p)|FD-Score:3.87|P-value:5.52E-5||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YOL046C(YOL046C_d)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W Gene:YOR121C(YOR121C_d)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YPQ1(YOL092W_p)|FD-Score:-3.14|P-value:8.57E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:YPR003C(YPR003C_p)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YSC84(YHR016C)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication Gene:AAT2(YLR027C)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ADH2(YMR303C)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 Gene:ADY2(YCR010C)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:AEP2(YMR282C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AIR1(YIL079C)|FD-Score:4.91|P-value:4.59E-7||SGD DESC:Zinc knuckle protein; involved in nuclear RNA processing and degradation as a component of the TRAMP complex; stimulates the poly(A) polymerase activity of Pap2p in vitro; AIR1 has a paralog, AIR2, that arose from the whole genome duplication Gene:BUB2(YMR055C)|FD-Score:6.15|P-value:3.92E-10||SGD DESC:Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage Gene:BUD30(YDL151C_d)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CCW12(YLR110C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CMC4(YMR194C-B)|FD-Score:7.08|P-value:7.08E-13||SGD DESC:Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs Gene:COG5(YNL051W)|FD-Score:5.75|P-value:4.57E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DCS1(YLR270W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DJP1(YIR004W)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ Gene:ECM25(YJL201W)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:ECM29(YHL030W)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ERG4(YGL012W)|FD-Score:-3.17|P-value:7.54E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FCY1(YPR062W)|FD-Score:5.63|P-value:9.07E-9||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FDC1(YDR539W)|FD-Score:4.81|P-value:7.39E-7||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:FLC3(YGL139W)|FD-Score:-3.13|P-value:8.85E-4||SGD DESC:Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER Gene:FRK1(YPL141C)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:GAS5(YOL030W)|FD-Score:-5.21|P-value:9.50E-8||SGD DESC:1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall Gene:GEP5(YLR091W)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:HOM6(YJR139C)|FD-Score:4.56|P-value:2.51E-6||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HPF1(YOL155C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:HSP31(YDR533C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:HTA1(YDR225W)|FD-Score:-3.28|P-value:5.26E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:HTZ1(YOL012C)|FD-Score:5.83|P-value:2.83E-9||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:IDH2(YOR136W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:JHD1(YER051W)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe Gene:KIN2(YLR096W)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:MBA1(YBR185C)|FD-Score:5.69|P-value:6.42E-9||SGD DESC:Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane Gene:MDM32(YOR147W)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MDY2(YOL111C)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MEF1(YLR069C)|FD-Score:3.81|P-value:6.84E-5||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MNN11(YJL183W)|FD-Score:3.93|P-value:4.21E-5||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MNS1(YJR131W)|FD-Score:-4|P-value:3.21E-5||SGD DESC:Alpha-1,2-mannosidase involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation Gene:MOS1(YCL057C-A)|FD-Score:5.39|P-value:3.48E-8||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MOT3(YMR070W)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MTH1(YDR277C)|FD-Score:3.77|P-value:8.21E-5||SGD DESC:Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation Gene:MUM3(YOR298W)|FD-Score:4.96|P-value:3.54E-7||SGD DESC:Protein of unknown function involved in the organization of the outer spore wall layers; has similarity to the tafazzins superfamily of acyltransferases Gene:NEW1(YPL226W)|FD-Score:4.31|P-value:8.16E-6||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NIT2(YJL126W)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:OPI3(YJR073C)|FD-Score:-3.11|P-value:9.48E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PAA1(YDR071C)|FD-Score:5.4|P-value:3.38E-8||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PAL1(YDR348C)|FD-Score:-3.31|P-value:4.66E-4||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PAR32(YDL173W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PDR18(YNR070W_p)|FD-Score:-4.08|P-value:2.22E-5||SGD DESC:Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PEX17(YNL214W)|FD-Score:3.88|P-value:5.13E-5||SGD DESC:Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis Gene:PHO81(YGR233C)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PSR2(YLR019W)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Functionally redundant Psr1p homolog, a plasma membrane phosphatase involved in the general stress response; required with Psr1p and Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:RAD27(YKL113C)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RCK1(YGL158W)|FD-Score:-3.12|P-value:8.94E-4||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:RIM101(YHL027W)|FD-Score:3.97|P-value:3.53E-5||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM9(YMR063W)|FD-Score:4.99|P-value:3.03E-7||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPL24A(YGL031C)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RTT106(YNL206C)|FD-Score:-4.25|P-value:1.05E-5||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:SIC1(YLR079W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SLP1(YOR154W_p)|FD-Score:4.47|P-value:3.88E-6||SGD DESC:Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p Gene:SLT2(YHR030C)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SMY2(YBR172C)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Protein of unknown function involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication Gene:SPF1(YEL031W)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SUR1(YPL057C)|FD-Score:-4.02|P-value:2.95E-5||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication Gene:TOM1(YDR457W)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TOM71(YHR117W)|FD-Score:4.87|P-value:5.44E-7||SGD DESC:Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication Gene:TRX3(YCR083W)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p Gene:UGA3(YDL170W)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:UPC2(YDR213W)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication Gene:VMR1(YHL035C)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:YBL100W-C(YBL100W-C_p)|FD-Score:-3.74|P-value:9.17E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCR050C(YCR050C_p)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YEL028W(YEL028W_d)|FD-Score:4.79|P-value:8.16E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR169C-A(YGR169C-A_p)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YHI9(YHR029C)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YIL054W(YIL054W_p)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YIL096C(YIL096C_p)|FD-Score:5.35|P-value:4.35E-8||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis Gene:YKL115C(YKL115C_d)|FD-Score:4.5|P-value:3.35E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YKR051W(YKR051W_p)|FD-Score:-3.15|P-value:8.19E-4||SGD DESC:Putative protein of unknown function Gene:YLR334C(YLR334C_d)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here Gene:YLR361C-A(YLR361C-A_p)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Putative protein of unknown function Gene:YMR034C(YMR034C_p)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YMR135W-A(YMR135W-A_d)|FD-Score:-3.18|P-value:7.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL050C(YNL050C_p)|FD-Score:3.87|P-value:5.52E-5||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YOL046C(YOL046C_d)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W Gene:YOR121C(YOR121C_d)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YPQ1(YOL092W_p)|FD-Score:-3.14|P-value:8.57E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:YPR003C(YPR003C_p)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YSC84(YHR016C)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR019C9.075.74E-205.30DED81Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA
YJL072C3.778.18E-50.27PSF2Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YLR229C3.502.31E-40.09CDC42Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins
YNL103W3.413.28E-40.10MET4Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p
YOR372C3.304.80E-40.00NDD1Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes
YFL029C3.304.82E-40.00CAK1Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases
YAL033W3.304.90E-40.01POP5Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YDR243C3.285.15E-40.03PRP28RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site
YDL209C3.255.82E-40.18CWC2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
YPL160W3.060.001100.01CDC60Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA
YOR095C3.060.001120.06RKI1Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis
YDL163W_d3.000.001360.01YDL163W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase
YIL091C2.980.001420.04UTP25Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis
YNL258C2.950.001610.08DSL1Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene
YBL092W2.860.002100.01RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR194C-B7.087.08E-13CMC4Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs
YMR055C6.153.92E-10BUB2Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage
YOL012C5.832.83E-9HTZ1Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin
YNL051W5.754.57E-9COG5Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YBR185C5.696.42E-9MBA1Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane
YPR062W5.639.07E-9FCY1Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU)
YDR071C5.403.38E-8PAA1Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication
YCL057C-A5.393.48E-8MOS1Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1
YIL096C_p5.354.35E-8YIL096C_pPutative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis
YMR063W4.993.03E-7RIM9Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI;
YOR298W4.963.54E-7MUM3Protein of unknown function involved in the organization of the outer spore wall layers; has similarity to the tafazzins superfamily of acyltransferases
YIL079C4.914.59E-7AIR1Zinc knuckle protein; involved in nuclear RNA processing and degradation as a component of the TRAMP complex; stimulates the poly(A) polymerase activity of Pap2p in vitro; AIR1 has a paralog, AIR2, that arose from the whole genome duplication
YHR117W4.875.44E-7TOM71Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication
YDR539W4.817.39E-7FDC1Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm
YEL028W_d4.798.16E-7YEL028W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_436
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0699.85E-8SGTC_29249010168 62.8 μMChembridge (Drug-like library)29939290.0147059cell wall signaling
0.0664.33E-7SGTC_492tmb-8 126.0 μMICCB bioactive library54940.0967742
0.0571.31E-5SGTC_30999120367 49.5 μMChembridge (Drug-like library)24824940NA
0.0535.19E-5SGTC_1737st035557 69.7 μMTimTec (Natural product derivative library)57169160.0655738
0.0519.51E-5SGTC_12740865-0114 173.0 μMChemDiv (Drug-like library)870624NA
0.0501.13E-4SGTC_24855633444 17.8 μMMiscellaneous57242390.0294118redox potentiating
0.0501.19E-4SGTC_2689tacrolimus 100.0 μMNIH Clinical Collection235817960.034188
0.0501.24E-4SGTC_30929117248 49.5 μMChembridge (Drug-like library)171418570.0133333cell wall signaling
0.0481.96E-4SGTC_31779107506 49.5 μMChembridge (Drug-like library)88971940.0277778ERG2
0.0464.12E-4SGTC_2731amoxapine 43.4 μMMiscellaneous2170NA
0.0454.93E-4SGTC_12760871-0068 94.2 μMChemDiv (Drug-like library)34149060.163265
0.0455.79E-4SGTC_3141671-0008 2.7 μMChemDiv (Drug-like library)39130600.0597015
0.0446.60E-4SGTC_5651418-0001 44.5 μMChemDiv (Drug-like library)28621450.016129
0.0446.95E-4SGTC_20605238662 140.0 μMChembridge (Fragment library)7901300.0566038
0.0438.56E-4SGTC_28909046729 58.4 μMChembridge (Drug-like library)9033290.0151515Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_438sphingosine6.67 μM0.6111115280335ICCB bioactive library299.491884.82433
SGTC_1573oleylamine1.06 μM0.3611115356789TimTec (Pure natural product library)267.493086.62311
SGTC_4732-arachidonoylglycerol10 μM0.3333335282280ICCB bioactive library378.545425.61424
SGTC_676falcarinol140 μM0.3333333322Miscellaneous244.371865.6811
SGTC_476mead acid10 μM0.3255815312531ICCB bioactive library306.482766.88312
SGTC_474dl-dihydrosphingosine5 μM0.3253058739ICCB bioactive library301.507765.13433
SGTC_477mead ethanolamide10 μM0.30612216061185ICCB bioactive library349.550565.92222
SGTC_1876myriocin605 nM0.303571301119Miscellaneous401.537460.28457
SGTC_409arachidonic acid100 μM0.302326231Miscellaneous304.466886.43912
SGTC_479n-linoleoylglycine10 μM0.286433346ICCB bioactive library337.49685.47623