fluspirilene FDA approved compound

8-[4,4-bis(4-fluorophenyl)butyl]-1-phenyl-1,3,8-triazaspiro[4.5]decan-4-one

An atypical antipsychotic.

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PubChem MeSH terms: Antipsychotic Agents;Dopamine Antagonists



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_440
Screen concentration 35.1 μM
Source ICCB bioactive library
PubChem CID 3396
SMILES C1CN(CCC12C(=O)NCN2C3=CC=CC=C3)CCCC(C4=CC=C(C=C4)F)C5=CC=C(C=C5)F
Standardized SMILES Fc1ccc(cc1)C(CCCN2CCC3(CC2)N(CNC3=O)c4ccccc4)c5ccc(F)cc5
Molecular weight 475.5727
ALogP 5.55
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.8
% growth inhibition (Hom. pool) 5.16


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3396
Download HIP data (tab-delimited text)  (excel)
Gene:CBF2(YGR140W)|FD-Score:-3.29|P-value:5.00E-4|Clearance:0||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:CIA2(YHR122W)|FD-Score:3.52|P-value:2.18E-4|Clearance:0.3||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B Gene:ECM16(YMR128W)|FD-Score:-3.19|P-value:7.10E-4|Clearance:0||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:FAS2(YPL231W)|FD-Score:-3.41|P-value:3.20E-4|Clearance:0||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:FRQ1(YDR373W)|FD-Score:3.11|P-value:9.51E-4|Clearance:0.02||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:HAS1(YMR290C)|FD-Score:3.65|P-value:1.30E-4|Clearance:0.14||SGD DESC:ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles Gene:MRD1(YPR112C)|FD-Score:-4.45|P-value:4.22E-6|Clearance:0||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:NOP7(YGR103W)|FD-Score:3.21|P-value:6.60E-4|Clearance:0.02||SGD DESC:Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G<sub>0</sub> and the initiation of cell proliferation Gene:QRI1(YDL103C)|FD-Score:-4.97|P-value:3.41E-7|Clearance:0||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:SEC23(YPR181C)|FD-Score:4.13|P-value:1.85E-5|Clearance:0.47||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:TFB1(YDR311W)|FD-Score:3.22|P-value:6.37E-4|Clearance:0.01||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:URA6(YKL024C)|FD-Score:3.19|P-value:7.01E-4|Clearance:0.09||SGD DESC:Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP) Gene:VRG4(YGL225W)|FD-Score:-4.05|P-value:2.58E-5|Clearance:0||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:YLR339C(YLR339C_d)|FD-Score:-4.8|P-value:7.91E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 Gene:CBF2(YGR140W)|FD-Score:-3.29|P-value:5.00E-4|Clearance:0||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:CIA2(YHR122W)|FD-Score:3.52|P-value:2.18E-4|Clearance:0.3||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B Gene:ECM16(YMR128W)|FD-Score:-3.19|P-value:7.10E-4|Clearance:0||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:FAS2(YPL231W)|FD-Score:-3.41|P-value:3.20E-4|Clearance:0||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:FRQ1(YDR373W)|FD-Score:3.11|P-value:9.51E-4|Clearance:0.02||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:HAS1(YMR290C)|FD-Score:3.65|P-value:1.30E-4|Clearance:0.14||SGD DESC:ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles Gene:MRD1(YPR112C)|FD-Score:-4.45|P-value:4.22E-6|Clearance:0||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:NOP7(YGR103W)|FD-Score:3.21|P-value:6.60E-4|Clearance:0.02||SGD DESC:Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G<sub>0</sub> and the initiation of cell proliferation Gene:QRI1(YDL103C)|FD-Score:-4.97|P-value:3.41E-7|Clearance:0||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:SEC23(YPR181C)|FD-Score:4.13|P-value:1.85E-5|Clearance:0.47||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:TFB1(YDR311W)|FD-Score:3.22|P-value:6.37E-4|Clearance:0.01||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:URA6(YKL024C)|FD-Score:3.19|P-value:7.01E-4|Clearance:0.09||SGD DESC:Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP) Gene:VRG4(YGL225W)|FD-Score:-4.05|P-value:2.58E-5|Clearance:0||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:YLR339C(YLR339C_d)|FD-Score:-4.8|P-value:7.91E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3396
Download HOP data (tab-delimited text)  (excel)
Gene:ADR1(YDR216W)|FD-Score:-4.71|P-value:1.21E-6||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:AGX1(YFL030W)|FD-Score:-4.59|P-value:2.20E-6||SGD DESC:Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases Gene:ALK1(YGL021W)|FD-Score:-3.19|P-value:7.02E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins Gene:ATP20(YPR020W)|FD-Score:-5.1|P-value:1.71E-7||SGD DESC:Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex Gene:AVO2(YMR068W)|FD-Score:-3.78|P-value:7.84E-5||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:BNI1(YNL271C)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BTN2(YGR142W)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:BXI1(YNL305C)|FD-Score:-3.92|P-value:4.34E-5||SGD DESC:Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p Gene:CAR2(YLR438W)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress Gene:CAT2(YML042W)|FD-Score:3.93|P-value:4.20E-5||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:CRF1(YDR223W)|FD-Score:3.86|P-value:5.77E-5||SGD DESC:Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain Gene:DDI3(YNL335W_p)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:DUF1(YOL087C)|FD-Score:-4.35|P-value:6.78E-6||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:DUG1(YFR044C)|FD-Score:3.74|P-value:9.19E-5||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:ELP6(YMR312W)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERG2(YMR202W)|FD-Score:5.26|P-value:7.05E-8||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FPR2(YDR519W)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:FUN14(YAL008W)|FD-Score:-5.33|P-value:4.90E-8||SGD DESC:Mitochondrial protein of unknown function Gene:FYV12(YOR183W_p)|FD-Score:-3.4|P-value:3.32E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GCV3(YAL044C)|FD-Score:4.32|P-value:7.69E-6||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GEP5(YLR091W)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:HCR1(YLR192C)|FD-Score:6.25|P-value:2.11E-10||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HIR3(YJR140C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein Gene:HRT3(YLR097C)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies Gene:ISM1(YPL040C)|FD-Score:3.89|P-value:5.03E-5||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:LAP3(YNL239W)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LEA1(YPL213W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:LRS4(YDR439W)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MAL32(YBR299W)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MPD2(YOL088C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRK1(YDL079C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MRL1(YPR079W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Membrane protein; has similarity to mammalian mannose-6-phosphate receptors; possibly functions as a sorting receptor in the delivery of vacuolar hydrolases; protein abundance increases in response to DNA replication stress Gene:NDE2(YDL085W)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain Gene:NPL3(YDR432W)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:OAF1(YAL051W)|FD-Score:4.66|P-value:1.57E-6||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:OXP1(YKL215C)|FD-Score:3.73|P-value:9.56E-5||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:PDR1(YGL013C)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PMS1(YNL082W)|FD-Score:3.73|P-value:9.47E-5||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PPS1(YBR276C)|FD-Score:-5.29|P-value:6.00E-8||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:PRS3(YHL011C)|FD-Score:3.96|P-value:3.78E-5||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:RBK1(YCR036W)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Putative ribokinase Gene:RIM4(YHL024W)|FD-Score:3.81|P-value:6.82E-5||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RMD1(YDL001W)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Cytoplasmic protein required for sporulation Gene:RNH70(YGR276C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:RPL26B(YGR034W)|FD-Score:-3.35|P-value:4.04E-4||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL43A(YPR043W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication Gene:RPL8B(YLL045C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:-4.23|P-value:1.17E-5||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RSM25(YIL093C)|FD-Score:-3.13|P-value:8.85E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTR1(YER139C)|FD-Score:4.06|P-value:2.49E-5||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SEG1(YMR086W)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SIC1(YLR079W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SNX3(YOR357C)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:SQS1(YNL224C)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:SSH1(YBR283C)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:UME1(YPL139C)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p Gene:VID24(YBR105C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VMA5(YKL080W)|FD-Score:5.48|P-value:2.15E-8||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VMA9(YCL005W-A)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:YAL037W(YAL037W_p)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication Gene:YCK3(YER123W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YDR444W(YDR444W_p)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YGL185C(YGL185C_p)|FD-Score:-4.36|P-value:6.54E-6||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGR111W(YGR111W_p)|FD-Score:-3.77|P-value:8.15E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YIR007W(YIR007W_p)|FD-Score:4.47|P-value:3.82E-6||SGD DESC:Putative glycosidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene Gene:YJL118W(YJL118W)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; YJL18W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein Gene:YKL118W(YKL118W_d)|FD-Score:5.47|P-value:2.23E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YML003W(YML003W_p)|FD-Score:-3.98|P-value:3.46E-5||SGD DESC:Putative protein of unknown function Gene:YML034C-A(YML034C-A_d)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML122C(YML122C_d)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR175W-A(YMR175W-A_p)|FD-Score:3.77|P-value:8.07E-5||SGD DESC:Putative protein of unknown function Gene:YMR193C-A(YMR193C-A_d)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR295C(YMR295C)|FD-Score:4.41|P-value:5.15E-6||SGD DESC:Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; YMR295C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPT7(YML001W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7 Gene:ADR1(YDR216W)|FD-Score:-4.71|P-value:1.21E-6||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:AGX1(YFL030W)|FD-Score:-4.59|P-value:2.20E-6||SGD DESC:Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases Gene:ALK1(YGL021W)|FD-Score:-3.19|P-value:7.02E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins Gene:ATP20(YPR020W)|FD-Score:-5.1|P-value:1.71E-7||SGD DESC:Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex Gene:AVO2(YMR068W)|FD-Score:-3.78|P-value:7.84E-5||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:BNI1(YNL271C)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BTN2(YGR142W)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:BXI1(YNL305C)|FD-Score:-3.92|P-value:4.34E-5||SGD DESC:Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p Gene:CAR2(YLR438W)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress Gene:CAT2(YML042W)|FD-Score:3.93|P-value:4.20E-5||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:CRF1(YDR223W)|FD-Score:3.86|P-value:5.77E-5||SGD DESC:Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain Gene:DDI3(YNL335W_p)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:DUF1(YOL087C)|FD-Score:-4.35|P-value:6.78E-6||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:DUG1(YFR044C)|FD-Score:3.74|P-value:9.19E-5||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:ELP6(YMR312W)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERG2(YMR202W)|FD-Score:5.26|P-value:7.05E-8||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FPR2(YDR519W)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:FUN14(YAL008W)|FD-Score:-5.33|P-value:4.90E-8||SGD DESC:Mitochondrial protein of unknown function Gene:FYV12(YOR183W_p)|FD-Score:-3.4|P-value:3.32E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GCV3(YAL044C)|FD-Score:4.32|P-value:7.69E-6||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GEP5(YLR091W)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:HCR1(YLR192C)|FD-Score:6.25|P-value:2.11E-10||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HIR3(YJR140C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein Gene:HRT3(YLR097C)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies Gene:ISM1(YPL040C)|FD-Score:3.89|P-value:5.03E-5||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:LAP3(YNL239W)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LEA1(YPL213W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:LRS4(YDR439W)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MAL32(YBR299W)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MPD2(YOL088C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRK1(YDL079C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MRL1(YPR079W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Membrane protein; has similarity to mammalian mannose-6-phosphate receptors; possibly functions as a sorting receptor in the delivery of vacuolar hydrolases; protein abundance increases in response to DNA replication stress Gene:NDE2(YDL085W)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain Gene:NPL3(YDR432W)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:OAF1(YAL051W)|FD-Score:4.66|P-value:1.57E-6||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:OXP1(YKL215C)|FD-Score:3.73|P-value:9.56E-5||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:PDR1(YGL013C)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PMS1(YNL082W)|FD-Score:3.73|P-value:9.47E-5||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PPS1(YBR276C)|FD-Score:-5.29|P-value:6.00E-8||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:PRS3(YHL011C)|FD-Score:3.96|P-value:3.78E-5||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:RBK1(YCR036W)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Putative ribokinase Gene:RIM4(YHL024W)|FD-Score:3.81|P-value:6.82E-5||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RMD1(YDL001W)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Cytoplasmic protein required for sporulation Gene:RNH70(YGR276C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:RPL26B(YGR034W)|FD-Score:-3.35|P-value:4.04E-4||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL43A(YPR043W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication Gene:RPL8B(YLL045C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:-4.23|P-value:1.17E-5||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RSM25(YIL093C)|FD-Score:-3.13|P-value:8.85E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTR1(YER139C)|FD-Score:4.06|P-value:2.49E-5||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SEG1(YMR086W)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SIC1(YLR079W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SNX3(YOR357C)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:SQS1(YNL224C)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:SSH1(YBR283C)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:UME1(YPL139C)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p Gene:VID24(YBR105C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VMA5(YKL080W)|FD-Score:5.48|P-value:2.15E-8||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VMA9(YCL005W-A)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:YAL037W(YAL037W_p)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication Gene:YCK3(YER123W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YDR444W(YDR444W_p)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YGL185C(YGL185C_p)|FD-Score:-4.36|P-value:6.54E-6||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGR111W(YGR111W_p)|FD-Score:-3.77|P-value:8.15E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YIR007W(YIR007W_p)|FD-Score:4.47|P-value:3.82E-6||SGD DESC:Putative glycosidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene Gene:YJL118W(YJL118W)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; YJL18W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein Gene:YKL118W(YKL118W_d)|FD-Score:5.47|P-value:2.23E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YML003W(YML003W_p)|FD-Score:-3.98|P-value:3.46E-5||SGD DESC:Putative protein of unknown function Gene:YML034C-A(YML034C-A_d)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML122C(YML122C_d)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR175W-A(YMR175W-A_p)|FD-Score:3.77|P-value:8.07E-5||SGD DESC:Putative protein of unknown function Gene:YMR193C-A(YMR193C-A_d)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR295C(YMR295C)|FD-Score:4.41|P-value:5.15E-6||SGD DESC:Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; YMR295C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPT7(YML001W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR181C4.131.85E-50.47SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
YMR290C3.651.30E-40.14HAS1ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles
YHR122W3.522.18E-40.29CIA2Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B
YDR311W3.226.37E-40.01TFB1Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators
YGR103W3.216.60E-40.02NOP7Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G0 and the initiation of cell proliferation
YKL024C3.197.01E-40.09URA6Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)
YDR373W3.119.51E-40.02FRQ1N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily
YGL099W3.090.001010.03LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YPR034W3.060.001120.01ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YHR166C3.040.001180.04CDC23Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YBR198C3.010.001330.09TAF5Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YPR016C2.920.001770.10TIF6Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
YBR123C2.810.002440.01TFC1One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63
YHR170W2.800.002520.02NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YPL082C2.780.002680.04MOT1Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR192C6.252.11E-10HCR1Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA
YKL080W5.482.15E-8VMA5Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane
YKL118W_d5.472.23E-8YKL118W_dDubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2
YMR202W5.267.05E-8ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YAL051W4.661.57E-6OAF1Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication
YIR007W_p4.473.82E-6YIR007W_pPutative glycosidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene
YMR295C4.415.15E-6YMR295CProtein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; YMR295C is not an essential gene; protein abundance increases in response to DNA replication stress
YAL044C4.327.69E-6GCV3H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF
YAL037W_p4.221.20E-5YAL037W_pPutative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication
YNL224C4.092.15E-5SQS1Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress
YER139C4.062.49E-5RTR1CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication
YHL011C3.963.78E-5PRS35-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes
YML042W3.934.20E-5CAT2Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes
YPL040C3.895.03E-5ISM1Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth
YDR223W3.865.77E-5CRF1Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain

GO enrichment analysis for SGTC_440
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1171.77E-19SGTC_5983937-0236 20.2 μMChemDiv (Drug-like library)13793130.0481928ERG2
0.1165.06E-19SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.0833333ERG2
0.1142.01E-18SGTC_2734clemastine 41.6 μMMiscellaneous269870.123457ERG2
0.1111.38E-17SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.0681818ERG2
0.1101.77E-17SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.0941176ERG2
0.1062.38E-16SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.0657895ERG2
0.1041.31E-15SGTC_1874staurosporine 7.6 μMICCB bioactive library468641930.11cell wall signaling
0.0983.63E-14SGTC_28629027634 71.4 μMChembridge (Drug-like library)50125620.0786517ERG2
0.0921.81E-12SGTC_20555235184 200.0 μMChembridge (Fragment library)28360340.102941ERG2
0.0921.82E-12SGTC_8410251-0215 91.2 μMChemDiv (Drug-like library)38886140.192982
0.0871.82E-11SGTC_12063978-0017 132.0 μMChemDiv (Drug-like library)38736770.123596ERG2
0.0862.91E-11SGTC_12440416-0015 52.5 μMChemDiv (Drug-like library)962010.0967742ERG2
0.0856.84E-11SGTC_28207989630 71.4 μMChembridge (Drug-like library)29840980.0795455ERG2
0.0841.02E-10SGTC_13601598-0022 39.8 μMChemDiv (Drug-like library)7615250.08ERG2
0.0831.49E-10SGTC_9741326-0144 138.0 μMChemDiv (Drug-like library)54118560.0694444ERG2

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2720fluspirilene36.88 μM13396Miscellaneous475.5727465.54515ERG2
SGTC_433pimozide108 μM0.46268716362Miscellaneous461.5461665.52114
SGTC_2249haloperidol116.36 μM0.2916673559Miscellaneous375.8642233.88714ergosterol depletion effects on membrane
SGTC_258haloperidol50.78 μM0.2916673559Miscellaneous375.8642233.88714fatty acid desaturase (OLE1)
SGTC_2725haloperidol53.21 μM0.2916673559Miscellaneous375.8642233.88714plasma membrane duress
SGTC_2732terfenadine20 μM0.270275405Miscellaneous471.673446.49823ERG2
SGTC_12830922-012516.4 μM0.253360884ChemDiv (Drug-like library)425.951024.84904
SGTC_10194262-02123.4 μM0.2285714677213ChemDiv (Drug-like library)352.4881635.20803ergosterol depletion effects on membrane
SGTC_3199911131849.47 μM0.2207798548020Chembridge (Drug-like library)381.3672733.73317
SGTC_2755lomerizine42.69 μM0.2162163949Miscellaneous468.5355065.41807
SGTC_14274048-4900550 nM0.21538524132586ChemDiv (Drug-like library)307.472365.79401
SGTC_2155557042376 μM0.215385797402Chembridge (Fragment library)250.3118632.54403ergosterol biosynthesis