clozapine FDA approved compound

3-chloro-6-(4-methylpiperazin-1-yl)-5H-benzo[b][1,4]benzodiazepine

An adrenergic, cholinergic, GABAA and histaminergic receptor agonist.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 

PubChem MeSH terms: Antipsychotic Agents;GABA Antagonists;Serotonin Antagonists



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_443
Screen concentration 122.0 μM
Source Miscellaneous
PubChem CID 2818
SMILES CN1CCN(CC1)C2=C3C=CC=CC3=NC4=C(N2)C=C(C=C4)Cl
Standardized SMILES CN1CCN(CC1)C2=Nc3cc(Cl)ccc3Nc4ccccc24
Molecular weight 326.8233
ALogP 3.48
H-bond donor count 1
H-bond acceptor count 4
Response signature NEO1-PIK1

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.28
% growth inhibition (Hom. pool) 5.11


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2818
Download HIP data (tab-delimited text)  (excel)
Gene:BUR6(YER159C)|FD-Score:3.19|P-value:7.01E-4|Clearance:0.03||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:LSG1(YGL099W)|FD-Score:3.16|P-value:7.88E-4|Clearance:0.42||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MOT1(YPL082C)|FD-Score:5.66|P-value:7.37E-9|Clearance:1.95||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:NEO1(YIL048W)|FD-Score:12.9|P-value:3.61E-38|Clearance:7.2||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NSE3(YDR288W)|FD-Score:3.49|P-value:2.42E-4|Clearance:0.01||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:RPT1(YKL145W)|FD-Score:3.53|P-value:2.05E-4|Clearance:0.04||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:SLD7(YOR060C)|FD-Score:3.72|P-value:1.02E-4|Clearance:0.18||SGD DESC:Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria Gene:SPC24(YMR117C)|FD-Score:3.48|P-value:2.48E-4|Clearance:0.23||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:SPT6(YGR116W)|FD-Score:3.24|P-value:5.96E-4|Clearance:0.05||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:TFG1(YGR186W)|FD-Score:3.25|P-value:5.70E-4|Clearance:0.01||SGD DESC:TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 Gene:VRG4(YGL225W)|FD-Score:-4.03|P-value:2.75E-5|Clearance:0||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:BUR6(YER159C)|FD-Score:3.19|P-value:7.01E-4|Clearance:0.03||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:LSG1(YGL099W)|FD-Score:3.16|P-value:7.88E-4|Clearance:0.42||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MOT1(YPL082C)|FD-Score:5.66|P-value:7.37E-9|Clearance:1.95||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:NEO1(YIL048W)|FD-Score:12.9|P-value:3.61E-38|Clearance:7.2||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NSE3(YDR288W)|FD-Score:3.49|P-value:2.42E-4|Clearance:0.01||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:RPT1(YKL145W)|FD-Score:3.53|P-value:2.05E-4|Clearance:0.04||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:SLD7(YOR060C)|FD-Score:3.72|P-value:1.02E-4|Clearance:0.18||SGD DESC:Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria Gene:SPC24(YMR117C)|FD-Score:3.48|P-value:2.48E-4|Clearance:0.23||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:SPT6(YGR116W)|FD-Score:3.24|P-value:5.96E-4|Clearance:0.05||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:TFG1(YGR186W)|FD-Score:3.25|P-value:5.70E-4|Clearance:0.01||SGD DESC:TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 Gene:VRG4(YGL225W)|FD-Score:-4.03|P-value:2.75E-5|Clearance:0||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2818
Download HOP data (tab-delimited text)  (excel)
Gene:ADE6(YGR061C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ATC1(YDR184C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:CCM1(YGR150C)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:COA1(YIL157C)|FD-Score:-3.13|P-value:8.77E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:COX23(YHR116W)|FD-Score:3.86|P-value:5.57E-5||SGD DESC:Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs Gene:CTS1(YLR286C)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p Gene:DYN3(YMR299C)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:ERG2(YMR202W)|FD-Score:4.97|P-value:3.38E-7||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERV14(YGL054C)|FD-Score:5.67|P-value:7.04E-9||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FMP41(YNL168C_p)|FD-Score:-4.09|P-value:2.20E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:HPR1(YDR138W)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:IST2(YBR086C)|FD-Score:-4.61|P-value:2.01E-6||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:LEM3(YNL323W)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LSM6(YDR378C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MAC1(YMR021C)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MGR2(YPL098C)|FD-Score:-3.13|P-value:8.64E-4||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MHF1(YOL086W-A)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 Gene:MSF1(YPR047W)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:NOT5(YPR072W)|FD-Score:-4.14|P-value:1.71E-5||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:OAC1(YKL120W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:PEP3(YLR148W)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:SNF1(YDR477W)|FD-Score:-4|P-value:3.12E-5||SGD DESC:AMP-activated serine/threonine protein kinase; found in a complex containing Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth in response to starvation Gene:SOL3(YHR163W)|FD-Score:-5.64|P-value:8.66E-9||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:SWS2(YNL081C)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TLG2(YOL018C)|FD-Score:4.94|P-value:3.95E-7||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA10(YLR327C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Protein of unknown function that associates with ribosomes; putative homolog of the F1F0-ATPase synthase regulator Stf2p; protein abundance increases in response to DNA replication stress Gene:TPS2(YDR074W)|FD-Score:-3.39|P-value:3.47E-4||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:UIP4(YPL186C)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:VMA9(YCL005W-A)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS1(YKR001C)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS29(YHR012W)|FD-Score:3.88|P-value:5.30E-5||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS35(YJL154C)|FD-Score:8.76|P-value:1.01E-18||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:YDR455C(YDR455C_d)|FD-Score:4.81|P-value:7.38E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YEF1(YEL041W)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YER158C(YER158C_p)|FD-Score:-4.06|P-value:2.42E-5||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YFR039C(YFR039C_p)|FD-Score:-3.13|P-value:8.81E-4||SGD DESC:Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces Gene:YGL072C(YGL072C_d)|FD-Score:4.95|P-value:3.71E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR149W(YGR149W_p)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Putative protein of unknown function; predicted to be an integal membrane protein Gene:YHR175W-A(YHR175W-A_p)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIG1(YPL201C)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YIR042C(YIR042C_p)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YJL068C(YJL068C)|FD-Score:3.81|P-value:6.89E-5||SGD DESC:Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D Gene:YMR262W(YMR262W_p)|FD-Score:-3.2|P-value:6.78E-4||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YPL229W(YPL229W_p)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:YPR084W(YPR084W_p)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Putative protein of unknown function Gene:YPR092W(YPR092W_d)|FD-Score:-3.25|P-value:5.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ADE6(YGR061C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ATC1(YDR184C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:CCM1(YGR150C)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:COA1(YIL157C)|FD-Score:-3.13|P-value:8.77E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:COX23(YHR116W)|FD-Score:3.86|P-value:5.57E-5||SGD DESC:Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs Gene:CTS1(YLR286C)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p Gene:DYN3(YMR299C)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:ERG2(YMR202W)|FD-Score:4.97|P-value:3.38E-7||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERV14(YGL054C)|FD-Score:5.67|P-value:7.04E-9||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FMP41(YNL168C_p)|FD-Score:-4.09|P-value:2.20E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:HPR1(YDR138W)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:IST2(YBR086C)|FD-Score:-4.61|P-value:2.01E-6||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:LEM3(YNL323W)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LSM6(YDR378C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MAC1(YMR021C)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MGR2(YPL098C)|FD-Score:-3.13|P-value:8.64E-4||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MHF1(YOL086W-A)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 Gene:MSF1(YPR047W)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:NOT5(YPR072W)|FD-Score:-4.14|P-value:1.71E-5||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:OAC1(YKL120W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:PEP3(YLR148W)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:SNF1(YDR477W)|FD-Score:-4|P-value:3.12E-5||SGD DESC:AMP-activated serine/threonine protein kinase; found in a complex containing Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth in response to starvation Gene:SOL3(YHR163W)|FD-Score:-5.64|P-value:8.66E-9||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:SWS2(YNL081C)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TLG2(YOL018C)|FD-Score:4.94|P-value:3.95E-7||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA10(YLR327C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Protein of unknown function that associates with ribosomes; putative homolog of the F1F0-ATPase synthase regulator Stf2p; protein abundance increases in response to DNA replication stress Gene:TPS2(YDR074W)|FD-Score:-3.39|P-value:3.47E-4||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:UIP4(YPL186C)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:VMA9(YCL005W-A)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS1(YKR001C)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS29(YHR012W)|FD-Score:3.88|P-value:5.30E-5||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS35(YJL154C)|FD-Score:8.76|P-value:1.01E-18||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:YDR455C(YDR455C_d)|FD-Score:4.81|P-value:7.38E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YEF1(YEL041W)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YER158C(YER158C_p)|FD-Score:-4.06|P-value:2.42E-5||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YFR039C(YFR039C_p)|FD-Score:-3.13|P-value:8.81E-4||SGD DESC:Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces Gene:YGL072C(YGL072C_d)|FD-Score:4.95|P-value:3.71E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR149W(YGR149W_p)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Putative protein of unknown function; predicted to be an integal membrane protein Gene:YHR175W-A(YHR175W-A_p)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIG1(YPL201C)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YIR042C(YIR042C_p)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YJL068C(YJL068C)|FD-Score:3.81|P-value:6.89E-5||SGD DESC:Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D Gene:YMR262W(YMR262W_p)|FD-Score:-3.2|P-value:6.78E-4||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YPL229W(YPL229W_p)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:YPR084W(YPR084W_p)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Putative protein of unknown function Gene:YPR092W(YPR092W_d)|FD-Score:-3.25|P-value:5.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIL048W12.903.61E-387.20NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YPL082C5.667.37E-91.95MOT1Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA
YOR060C3.721.02E-40.18SLD7Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria
YKL145W3.532.05E-40.04RPT1One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
YDR288W3.492.42E-40.01NSE3Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress
YMR117C3.482.48E-40.23SPC24Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
YGR186W3.255.70E-40.01TFG1TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74
YGR116W3.245.96E-40.05SPT6Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region
YER159C3.197.01E-40.03BUR6Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha
YGL099W3.167.88E-40.42LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YMR076C2.740.003064.64E-4PDS5Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis
YLR117C2.740.003070.05CLF1Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
YLL031C2.690.003520.12GPI13ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein
YLR305C2.570.005020.01STT4Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization
YOL127W2.570.005130.00RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL154C8.761.01E-18VPS35Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
YGL054C5.677.04E-9ERV14COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
YPL229W_p5.151.31E-7YPL229W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication
YMR202W4.973.38E-7ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YGL072C_d4.953.71E-7YGL072C_dDubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
YOL018C4.943.95E-7TLG2Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
YDR455C_d4.817.38E-7YDR455C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W
YLR148W4.751.02E-6PEP3Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis
YLR286C4.121.89E-5CTS1Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p
YHR175W-A_p3.953.90E-5YHR175W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YHR012W3.885.30E-5VPS29Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
YHR116W3.865.57E-5COX23Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs
YJL068C3.816.89E-5YJL068CNon-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D
YDR138W3.671.24E-4HPR1Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p
YDR378C3.611.55E-4LSM6Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA

GO enrichment analysis for SGTC_443
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4235.60E-255SGTC_5352293-4129 48.2 μMChemDiv (Drug-like library)3961180.0875NEO1
0.4043.93E-231SGTC_232clozapine 119.0 μMMiscellaneous28181NEO1
0.4014.35E-227SGTC_3014119-0100 24.5 μMChemDiv (Drug-like library)28010.215385NEO1
0.3953.18E-220SGTC_8880916-0002 7.0 μMChemDiv (Drug-like library)28021380.181818NEO1
0.3929.32E-216SGTC_1665st014639 11.4 μMTimTec (Natural product derivative library)9811330.0989011NEO1
0.3773.31E-199SGTC_9001112-0045 141.0 μMChemDiv (Drug-like library)562110.121212NEO1
0.3695.61E-190SGTC_1991st073000 8.9 μMTimTec (Natural product derivative library)43019690.11236NEO1
0.3589.80E-178SGTC_2697mebhydrolin 72.4 μMTimTec (Natural product derivative library)225300.164179NEO1
0.3546.00E-174SGTC_1721112-0046 2.6 μMChemDiv (Drug-like library)28021350.181818NEO1
0.3541.24E-173SGTC_2658polymyxin b sulfate 77.0 μMMicrosource (Natural product library)57021050.0650406NEO1
0.3533.34E-173SGTC_1781st038725 66.3 μMTimTec (Natural product derivative library)42702750.149254NEO1
0.3385.17E-158SGTC_270tamoxifen 5.5 μMMiscellaneous53760.0857143NEO1
0.3356.88E-155SGTC_2064546-0025 72.0 μMChemDiv (Drug-like library)66207450.131579NEO1
0.3331.47E-152SGTC_9403232-1287 54.8 μMChemDiv (Drug-like library)15854930.0581395NEO1
0.3234.10E-143SGTC_449xestospongin c 11.2 μMICCB bioactive library53115020.047619NEO1-PIK1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_232clozapine119 μM12818Miscellaneous326.823263.47614NEO1
SGTC_2742clothiapine36.27 μM0.5471716351Miscellaneous343.873623.99103NEO1
SGTC_2731amoxapine43.43 μM0.4137932170Miscellaneous313.781442.89713
SGTC_235890371574 μM0.3636362388925Chembridge (Fragment library)231.250520.20513
SGTC_1504k297-003330 μM0.3428574388117ChemDiv (Drug-like library)413.966764.69123
SGTC_11303454-285815.9 μM0.338983715628ChemDiv (Drug-like library)338.833964.33104NEO1
SGTC_3280914096149.47 μM0.3230774901226Chembridge (Drug-like library)355.864483.5090460S ribosome export
SGTC_282k297-003665.7 μM0.3064525061378ChemDiv (Drug-like library)301.793884.44421
SGTC_3393091-483371.4 μM0.3050853240077ChemDiv (Drug-like library)272.686463.1713iron homeostasis
SGTC_12690831-07963.05 μM0.3927758ChemDiv (Drug-like library)268.697763.1170160S ribosome export