monastrol

ethyl 4-(3-hydroxyphenyl)-6-methyl-2-sulfanylidene-3,4-dihydro-1H-pyrimidine-5-carboxylate

Monastrol is a cell-permeable small molecule inhibitor discovered by Thomas U. Mayer in the lab of Tim Mitchison. Monastrol was shown to inhibit the kinesin Eg5, a motor protein important for spindle bipolarity

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_448
Screen concentration 171.0 μM
Source ICCB bioactive library
PubChem CID 2987927
SMILES CCOC(=O)C1=C(NC(=S)NC1C2=CC(=CC=C2)O)C
Standardized SMILES CCOC(=C1C(NC(=S)N=C1C)c2cccc(O)c2)O
Molecular weight 292.3534
ALogP 2.46
H-bond donor count 3
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.71
% growth inhibition (Hom. pool) 10.43


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2987927
Download HIP data (tab-delimited text)  (excel)
Gene:MRD1(YPR112C)|FD-Score:3.41|P-value:3.23E-4|Clearance:0.05||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:RPP0(YLR340W)|FD-Score:4.37|P-value:6.34E-6|Clearance:0.95||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:SEC31(YDL195W)|FD-Score:3.11|P-value:9.23E-4|Clearance:0.04||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SRM1(YGL097W)|FD-Score:3.36|P-value:3.86E-4|Clearance:0.25||SGD DESC:Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p Gene:MRD1(YPR112C)|FD-Score:3.41|P-value:3.23E-4|Clearance:0.05||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:RPP0(YLR340W)|FD-Score:4.37|P-value:6.34E-6|Clearance:0.95||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:SEC31(YDL195W)|FD-Score:3.11|P-value:9.23E-4|Clearance:0.04||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SRM1(YGL097W)|FD-Score:3.36|P-value:3.86E-4|Clearance:0.25||SGD DESC:Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2987927
Download HOP data (tab-delimited text)  (excel)
Gene:ADE6(YGR061C)|FD-Score:-4.57|P-value:2.47E-6||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ARG3(YJL088W)|FD-Score:-3.14|P-value:8.48E-4||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ASN2(YGR124W)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:ATG2(YNL242W)|FD-Score:6.8|P-value:5.38E-12||SGD DESC:Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress Gene:ATO3(YDR384C)|FD-Score:4.57|P-value:2.48E-6||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:BLS1(YLR408C_p)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene Gene:BUD28(YLR062C_d)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:DPB4(YDR121W)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization Gene:ECI1(YLR284C)|FD-Score:3.99|P-value:3.36E-5||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:ECT1(YGR007W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases Gene:FRM2(YCL026C-A)|FD-Score:-3.17|P-value:7.72E-4||SGD DESC:Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin Gene:GEX2(YKR106W)|FD-Score:-4.05|P-value:2.59E-5||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GIP3(YPL137C)|FD-Score:-4.75|P-value:9.95E-7||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:MDJ2(YNL328C)|FD-Score:5.71|P-value:5.68E-9||SGD DESC:Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain Gene:MDL2(YPL270W)|FD-Score:3.78|P-value:7.91E-5||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MEI4(YER044C-A)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MHF1(YOL086W-A)|FD-Score:-5.7|P-value:5.88E-9||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 Gene:MSB2(YGR014W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSH4(YFL003C)|FD-Score:3.82|P-value:6.79E-5||SGD DESC:Protein involved in meiotic recombination, required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein Gene:OCA2(YNL056W)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:PIG1(YLR273C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase Gene:PUN1(YLR414C)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress Gene:RPN14(YGL004C)|FD-Score:6.32|P-value:1.30E-10||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p Gene:SKI2(YLR398C)|FD-Score:4.94|P-value:3.86E-7||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:STD1(YOR047C)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:TUB3(YML124C)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:ULI1(YFR026C)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Putative protein of unknown function involved in and induced by the endoplasmic reticulum unfolded protein response Gene:VMS1(YDR049W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:VPS73(YGL104C)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:YDL034W(YDL034W_d)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene Gene:YDL177C(YDL177C_p)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YDR015C(YDR015C_d)|FD-Score:-3.12|P-value:8.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YGR021W(YGR021W_p)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YGR139W(YGR139W_d)|FD-Score:4.66|P-value:1.59E-6||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR011C(YJR011C_p)|FD-Score:-3.12|P-value:9.18E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS Gene:YLR311C(YLR311C_d)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR415C(YLR415C_p)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Putative protein of unknown function; YLR415C is not an essential gene Gene:YNL276C(YNL276C_d)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YOR289W(YOR289W_p)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPL077C(YPL077C_p)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:ADE6(YGR061C)|FD-Score:-4.57|P-value:2.47E-6||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ARG3(YJL088W)|FD-Score:-3.14|P-value:8.48E-4||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ASN2(YGR124W)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:ATG2(YNL242W)|FD-Score:6.8|P-value:5.38E-12||SGD DESC:Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress Gene:ATO3(YDR384C)|FD-Score:4.57|P-value:2.48E-6||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:BLS1(YLR408C_p)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene Gene:BUD28(YLR062C_d)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:DPB4(YDR121W)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization Gene:ECI1(YLR284C)|FD-Score:3.99|P-value:3.36E-5||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:ECT1(YGR007W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases Gene:FRM2(YCL026C-A)|FD-Score:-3.17|P-value:7.72E-4||SGD DESC:Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin Gene:GEX2(YKR106W)|FD-Score:-4.05|P-value:2.59E-5||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GIP3(YPL137C)|FD-Score:-4.75|P-value:9.95E-7||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:MDJ2(YNL328C)|FD-Score:5.71|P-value:5.68E-9||SGD DESC:Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain Gene:MDL2(YPL270W)|FD-Score:3.78|P-value:7.91E-5||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MEI4(YER044C-A)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MHF1(YOL086W-A)|FD-Score:-5.7|P-value:5.88E-9||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 Gene:MSB2(YGR014W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSH4(YFL003C)|FD-Score:3.82|P-value:6.79E-5||SGD DESC:Protein involved in meiotic recombination, required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein Gene:OCA2(YNL056W)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:PIG1(YLR273C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase Gene:PUN1(YLR414C)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress Gene:RPN14(YGL004C)|FD-Score:6.32|P-value:1.30E-10||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p Gene:SKI2(YLR398C)|FD-Score:4.94|P-value:3.86E-7||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:STD1(YOR047C)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:TUB3(YML124C)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:ULI1(YFR026C)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Putative protein of unknown function involved in and induced by the endoplasmic reticulum unfolded protein response Gene:VMS1(YDR049W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:VPS73(YGL104C)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:YDL034W(YDL034W_d)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene Gene:YDL177C(YDL177C_p)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YDR015C(YDR015C_d)|FD-Score:-3.12|P-value:8.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YGR021W(YGR021W_p)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YGR139W(YGR139W_d)|FD-Score:4.66|P-value:1.59E-6||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR011C(YJR011C_p)|FD-Score:-3.12|P-value:9.18E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS Gene:YLR311C(YLR311C_d)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR415C(YLR415C_p)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Putative protein of unknown function; YLR415C is not an essential gene Gene:YNL276C(YNL276C_d)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YOR289W(YOR289W_p)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPL077C(YPL077C_p)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR340W4.376.34E-60.95RPP0Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10
YPR112C3.413.23E-40.05MRD1Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region
YGL097W3.363.86E-40.25SRM1Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p
YDL195W3.119.23E-40.04SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YPR035W3.070.001070.06GLN1Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YPR085C3.010.001320.03ASA1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
YGL061C2.980.001440.07DUO1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YGR094W2.910.001780.00VAS1Mitochondrial and cytoplasmic valyl-tRNA synthetase
YDL084W2.910.001800.11SUB2Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress
YLR459W2.800.002560.05GAB1GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI
YJL156C2.750.002980.03SSY5Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p
YKL058W2.720.003300.02TOA2TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
YOR116C2.700.003480.06RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YPL146C2.640.004200.04NOP53Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired
YLR147C2.600.004660.08SMD3Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL242W6.805.38E-12ATG2Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress
YGL004C6.321.30E-10RPN14Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p
YNL328C5.715.68E-9MDJ2Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain
YLR398C4.943.86E-7SKI2Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay
YGR139W_d4.661.59E-6YGR139W_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDR384C4.572.48E-6ATO3Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters
YGR021W_p4.251.05E-5YGR021W_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YGR014W4.211.30E-5MSB2Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate
YLR414C4.013.01E-5PUN1Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress
YLR284C3.993.36E-5ECI1Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced
YFL003C3.826.79E-5MSH4Protein involved in meiotic recombination, required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein
YPL270W3.787.91E-5MDL2Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis
YGR007W3.591.63E-4ECT1Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases
YER044C-A3.591.66E-4MEI4Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores
YDR049W3.571.76E-4VMS1Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans

GO enrichment analysis for SGTC_448
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1817.55E-44SGTC_460trequinsin 123.0 μMMiscellaneous55370.0813954
0.1303.65E-23SGTC_461N-acetylfarnesylcysteine 136.0 μMICCB bioactive library64383810.0779221
0.0995.08E-14SGTC_60844-0013 19.8 μMChemDiv (Drug-like library)15849000.08
0.0962.58E-13SGTC_246cycloheximide 667.0 μMICCB bioactive library167600400.111111
0.0874.08E-11SGTC_8940960-0238 55.9 μMChemDiv (Drug-like library)67419070.13253
0.0859.96E-11SGTC_20995377090 177.4 μMChembridge (Fragment library)22438290.0694444
0.0714.79E-8SGTC_1724st037281 8.3 μMTimTec (Natural product derivative library)5741240.133333
0.0664.37E-7SGTC_1994st072947 40.9 μMTimTec (Natural product derivative library)52865650.121951
0.0664.98E-7SGTC_30519093778 49.5 μMChembridge (Drug-like library)172173780.171053
0.0649.91E-7SGTC_23839036125 200.0 μMChembridge (Fragment library)795800.111111
0.0641.15E-6SGTC_249418-aminoabieta-8,11,13-triene sulfate 3.7 μMMicrosource (Natural product library)167250760.0875
0.0622.64E-6SGTC_521r062-0005 13.8 μMChemDiv (Drug-like library)28320330.084507
0.0588.14E-6SGTC_31659101544 49.5 μMChembridge (Drug-like library)50021840.123288
0.0561.83E-5SGTC_12680828-0275 2.5 μMChemDiv (Drug-like library)7453370.0972222
0.0561.93E-5SGTC_1870st057514 26.5 μMTimTec (Natural product derivative library)53931610.15493RSC & ERG11

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_488nitrendipine139 μM0.43754507Miscellaneous360.36122.11517
SGTC_2740felodipine17.08 μM0.3968253333Miscellaneous384.253763.54915
SGTC_2406ethylparaben405.7 μM0.3529418434Miscellaneous166.17391.79213
SGTC_2713st07914953.4 μM0.3472223103253TimTec (Natural product derivative library)374.430842.91635
SGTC_2626ethyl orsellinate100 μM0.34545575653Microsource (Natural product library)196.199882.03624
SGTC_2113455-0679143.5 μM0.327869748649ChemDiv (Drug-like library)220.221282.87314
SGTC_487nimodipine119 μM0.3243244497Miscellaneous418.440342.36118
SGTC_2506ethyl hematommate54.16 μM0.2950823940691Microsource (Natural product library)224.209981.79525RPP1 & pyrimidine depletion
SGTC_14843473-2379286 μM0.283582671917ChemDiv (Drug-like library)279.311621.89825
SGTC_7161683-5115658 μM0.276923723597ChemDiv (Drug-like library)303.740263.48313