aminopyrene

pyren-1-amine

Aminopyrene is an established DNA mutagen.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_450
Screen concentration 156.0 nM
Source Miscellaneous
PubChem CID 15352
SMILES C1=CC2=C3C(=C1)C=CC4=C(C=CC(=C43)C=C2)N
Standardized SMILES Nc1ccc2ccc3cccc4ccc1c2c34
Molecular weight 217.2652
ALogP 3.2
H-bond donor count 1
H-bond acceptor count 1
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 21.19
% growth inhibition (Hom. pool) 17.08


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 15352
Download HIP data (tab-delimited text)  (excel)
Gene:DML1(YMR211W)|FD-Score:-3.54|P-value:1.96E-4|Clearance:0||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:FCF1(YDR339C)|FD-Score:3.16|P-value:7.98E-4|Clearance:0.04||SGD DESC:Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p Gene:GCD14(YJL125C)|FD-Score:4.19|P-value:1.40E-5|Clearance:0.05||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:MCM7(YBR202W)|FD-Score:4.31|P-value:8.32E-6|Clearance:0.02||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:PKC1(YBL105C)|FD-Score:4.57|P-value:2.49E-6|Clearance:0.26||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:POL3(YDL102W)|FD-Score:4.28|P-value:9.15E-6|Clearance:0.09||SGD DESC:Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) Gene:PRE2(YPR103W)|FD-Score:4.68|P-value:1.46E-6|Clearance:0.11||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RET2(YFR051C)|FD-Score:3.12|P-value:9.00E-4|Clearance:0.02||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RFC2(YJR068W)|FD-Score:-3.69|P-value:1.14E-4|Clearance:0||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RHO1(YPR165W)|FD-Score:16.2|P-value:5.40E-59|Clearance:11.48||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RHO3(YIL118W)|FD-Score:4.14|P-value:1.76E-5|Clearance:0.69||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RPC10(YHR143W-A)|FD-Score:3.1|P-value:9.64E-4|Clearance:0.01||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPL17A(YKL180W)|FD-Score:-3.87|P-value:5.53E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SDH3(YKL141W)|FD-Score:3.09|P-value:9.99E-4|Clearance:0.25||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SEC12(YNR026C)|FD-Score:3.38|P-value:3.66E-4|Clearance:0.01||SGD DESC:Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER Gene:SEC23(YPR181C)|FD-Score:3.45|P-value:2.82E-4|Clearance:0.07||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:TFC6(YDR362C)|FD-Score:-3.37|P-value:3.78E-4|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TIF35(YDR429C)|FD-Score:-3.17|P-value:7.55E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YPL251W(YPL251W_d)|FD-Score:3.36|P-value:3.86E-4|Clearance:0.21||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C Gene:DML1(YMR211W)|FD-Score:-3.54|P-value:1.96E-4|Clearance:0||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:FCF1(YDR339C)|FD-Score:3.16|P-value:7.98E-4|Clearance:0.04||SGD DESC:Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p Gene:GCD14(YJL125C)|FD-Score:4.19|P-value:1.40E-5|Clearance:0.05||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:MCM7(YBR202W)|FD-Score:4.31|P-value:8.32E-6|Clearance:0.02||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:PKC1(YBL105C)|FD-Score:4.57|P-value:2.49E-6|Clearance:0.26||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:POL3(YDL102W)|FD-Score:4.28|P-value:9.15E-6|Clearance:0.09||SGD DESC:Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) Gene:PRE2(YPR103W)|FD-Score:4.68|P-value:1.46E-6|Clearance:0.11||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RET2(YFR051C)|FD-Score:3.12|P-value:9.00E-4|Clearance:0.02||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RFC2(YJR068W)|FD-Score:-3.69|P-value:1.14E-4|Clearance:0||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RHO1(YPR165W)|FD-Score:16.2|P-value:5.40E-59|Clearance:11.48||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RHO3(YIL118W)|FD-Score:4.14|P-value:1.76E-5|Clearance:0.69||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RPC10(YHR143W-A)|FD-Score:3.1|P-value:9.64E-4|Clearance:0.01||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPL17A(YKL180W)|FD-Score:-3.87|P-value:5.53E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SDH3(YKL141W)|FD-Score:3.09|P-value:9.99E-4|Clearance:0.25||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SEC12(YNR026C)|FD-Score:3.38|P-value:3.66E-4|Clearance:0.01||SGD DESC:Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER Gene:SEC23(YPR181C)|FD-Score:3.45|P-value:2.82E-4|Clearance:0.07||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:TFC6(YDR362C)|FD-Score:-3.37|P-value:3.78E-4|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TIF35(YDR429C)|FD-Score:-3.17|P-value:7.55E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YPL251W(YPL251W_d)|FD-Score:3.36|P-value:3.86E-4|Clearance:0.21||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 15352
Download HOP data (tab-delimited text)  (excel)
Gene:AKR1(YDR264C)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ALF1(YNL148C)|FD-Score:5.43|P-value:2.78E-8||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ALY1(YKR021W)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication Gene:APL3(YBL037W)|FD-Score:5.04|P-value:2.38E-7||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:APN1(YKL114C)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARG82(YDR173C)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ARO1(YDR127W)|FD-Score:6.17|P-value:3.44E-10||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARP1(YHR129C)|FD-Score:4.83|P-value:6.95E-7||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:BCK1(YJL095W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CAN1(YEL063C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CDC50(YCR094W)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CHS6(YJL099W)|FD-Score:-3.78|P-value:7.74E-5||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:COS10(YNR075W)|FD-Score:4|P-value:3.11E-5||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CUE1(YMR264W)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:CYS3(YAL012W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DBR1(YKL149C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition Gene:DMA2(YNL116W)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains Gene:DYN2(YDR424C)|FD-Score:-3.2|P-value:6.86E-4||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:ELP3(YPL086C)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:ENV7(YPL236C)|FD-Score:7.04|P-value:9.83E-13||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:ERG2(YMR202W)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG3(YLR056W)|FD-Score:4.31|P-value:8.19E-6||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:ERG5(YMR015C)|FD-Score:7.87|P-value:1.84E-15||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:ERV46(YAL042W)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FYV10(YIL097W)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GCN2(YDR283C)|FD-Score:4.42|P-value:5.04E-6||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GUD1(YDL238C)|FD-Score:3.1|P-value:9.51E-4||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HAP4(YKL109W)|FD-Score:-3.76|P-value:8.57E-5||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HEF3(YNL014W)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:HTA1(YDR225W)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:ISY1(YJR050W)|FD-Score:4.49|P-value:3.48E-6||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:KAP120(YPL125W)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:LOC1(YFR001W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:LOT5(YKL183W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress Gene:MNS1(YJR131W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Alpha-1,2-mannosidase involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation Gene:MPD2(YOL088C)|FD-Score:3.73|P-value:9.46E-5||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRH4(YGL064C)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRN1(YPL184C)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:NOT5(YPR072W)|FD-Score:5.05|P-value:2.21E-7||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:OGG1(YML060W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:OPI3(YJR073C)|FD-Score:5.16|P-value:1.21E-7||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OPI9(YLR338W_d)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PCI8(YIL071C)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PET494(YNR045W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane Gene:PHO88(YBR106W)|FD-Score:15.1|P-value:7.24E-52||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PNS1(YOR161C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport Gene:PTC7(YHR076W)|FD-Score:5.68|P-value:6.70E-9||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:RAD23(YEL037C)|FD-Score:4.69|P-value:1.36E-6||SGD DESC:Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover Gene:RAD50(YNL250W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress Gene:RBK1(YCR036W)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Putative ribokinase Gene:ROD1(YOR018W)|FD-Score:3.86|P-value:5.75E-5||SGD DESC:Membrane protein, binds the ubiquitin ligase Rsp5p via its 2 PY motifs; overexpression confers resistance to the GST substrate o-dinitrobenzene, zinc, and calcium; proposed to regulate the endocytosis of plasma membrane proteins; protein abundance increases in response to DNA replication stress; ROD1 has a paralog, ROG3, that arose from the whole genome duplication Gene:RPL17B(YJL177W)|FD-Score:4.32|P-value:7.67E-6||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:7.47|P-value:3.97E-14||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAC6(YDR129C)|FD-Score:-3.82|P-value:6.71E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SEC28(YIL076W)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress Gene:SHE4(YOR035C)|FD-Score:-3.72|P-value:9.79E-5||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIL1(YOL031C)|FD-Score:4.39|P-value:5.80E-6||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SKN7(YHR206W)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLP1(YOR154W_p)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p Gene:SRO77(YBL106C)|FD-Score:3.76|P-value:8.48E-5||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:STB3(YDR169C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress Gene:TAT1(YBR069C)|FD-Score:6.09|P-value:5.71E-10||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA6(YPR157W_p)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:TPK2(YPL203W)|FD-Score:-4.45|P-value:4.37E-6||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TRM12(YML005W)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA Gene:TRP3(YKL211C)|FD-Score:5.17|P-value:1.18E-7||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSR2(YLR435W)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:TUB3(YML124C)|FD-Score:6.21|P-value:2.61E-10||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBC7(YMR022W)|FD-Score:6.4|P-value:7.93E-11||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:VID22(YLR373C)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:WSS1(YHR134W)|FD-Score:4.43|P-value:4.72E-6||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YBL029C-A(YBL029C-A_p)|FD-Score:-4.33|P-value:7.60E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica Gene:YBL071C-B(YBL071C-B_p)|FD-Score:5.61|P-value:9.93E-9||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR277C(YBR277C_d)|FD-Score:5.2|P-value:9.70E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCR102C(YCR102C_p)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDL118W(YDL118W_p)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR008C(YDR008C_d)|FD-Score:5.16|P-value:1.26E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR201C(YGR201C_p)|FD-Score:-3.15|P-value:8.14E-4||SGD DESC:Putative protein of unknown function Gene:YHR022C-A(YHR022C-A_p)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YIL054W(YIL054W_p)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YIR035C(YIR035C_p)|FD-Score:-3.13|P-value:8.67E-4||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YJL135W(YJL135W_d)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3 Gene:YJR151W-A(YJR151W-A_p)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational guilt by association analysis Gene:YKR045C(YKR045C_p)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YMR031W-A(YMR031W-A_d)|FD-Score:4.08|P-value:2.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR196W(YMR196W_p)|FD-Score:3.75|P-value:8.98E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YOL073C(YOL073C_p)|FD-Score:3.98|P-value:3.45E-5||SGD DESC:Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2 Gene:YOR338W(YOR338W_p)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication Gene:YPL080C(YPL080C_d)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR084W(YPR084W_p)|FD-Score:-4.68|P-value:1.43E-6||SGD DESC:Putative protein of unknown function Gene:AKR1(YDR264C)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ALF1(YNL148C)|FD-Score:5.43|P-value:2.78E-8||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ALY1(YKR021W)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication Gene:APL3(YBL037W)|FD-Score:5.04|P-value:2.38E-7||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:APN1(YKL114C)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARG82(YDR173C)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ARO1(YDR127W)|FD-Score:6.17|P-value:3.44E-10||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARP1(YHR129C)|FD-Score:4.83|P-value:6.95E-7||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:BCK1(YJL095W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CAN1(YEL063C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CDC50(YCR094W)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CHS6(YJL099W)|FD-Score:-3.78|P-value:7.74E-5||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:COS10(YNR075W)|FD-Score:4|P-value:3.11E-5||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CUE1(YMR264W)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:CYS3(YAL012W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DBR1(YKL149C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition Gene:DMA2(YNL116W)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains Gene:DYN2(YDR424C)|FD-Score:-3.2|P-value:6.86E-4||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:ELP3(YPL086C)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:ENV7(YPL236C)|FD-Score:7.04|P-value:9.83E-13||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:ERG2(YMR202W)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG3(YLR056W)|FD-Score:4.31|P-value:8.19E-6||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:ERG5(YMR015C)|FD-Score:7.87|P-value:1.84E-15||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:ERV46(YAL042W)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FYV10(YIL097W)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GCN2(YDR283C)|FD-Score:4.42|P-value:5.04E-6||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GUD1(YDL238C)|FD-Score:3.1|P-value:9.51E-4||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HAP4(YKL109W)|FD-Score:-3.76|P-value:8.57E-5||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HEF3(YNL014W)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:HTA1(YDR225W)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:ISY1(YJR050W)|FD-Score:4.49|P-value:3.48E-6||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:KAP120(YPL125W)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:LOC1(YFR001W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:LOT5(YKL183W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress Gene:MNS1(YJR131W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Alpha-1,2-mannosidase involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation Gene:MPD2(YOL088C)|FD-Score:3.73|P-value:9.46E-5||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRH4(YGL064C)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRN1(YPL184C)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:NOT5(YPR072W)|FD-Score:5.05|P-value:2.21E-7||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:OGG1(YML060W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:OPI3(YJR073C)|FD-Score:5.16|P-value:1.21E-7||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OPI9(YLR338W_d)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PCI8(YIL071C)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PET494(YNR045W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane Gene:PHO88(YBR106W)|FD-Score:15.1|P-value:7.24E-52||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PNS1(YOR161C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport Gene:PTC7(YHR076W)|FD-Score:5.68|P-value:6.70E-9||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:RAD23(YEL037C)|FD-Score:4.69|P-value:1.36E-6||SGD DESC:Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover Gene:RAD50(YNL250W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress Gene:RBK1(YCR036W)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Putative ribokinase Gene:ROD1(YOR018W)|FD-Score:3.86|P-value:5.75E-5||SGD DESC:Membrane protein, binds the ubiquitin ligase Rsp5p via its 2 PY motifs; overexpression confers resistance to the GST substrate o-dinitrobenzene, zinc, and calcium; proposed to regulate the endocytosis of plasma membrane proteins; protein abundance increases in response to DNA replication stress; ROD1 has a paralog, ROG3, that arose from the whole genome duplication Gene:RPL17B(YJL177W)|FD-Score:4.32|P-value:7.67E-6||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:7.47|P-value:3.97E-14||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAC6(YDR129C)|FD-Score:-3.82|P-value:6.71E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SEC28(YIL076W)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress Gene:SHE4(YOR035C)|FD-Score:-3.72|P-value:9.79E-5||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIL1(YOL031C)|FD-Score:4.39|P-value:5.80E-6||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SKN7(YHR206W)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLP1(YOR154W_p)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p Gene:SRO77(YBL106C)|FD-Score:3.76|P-value:8.48E-5||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:STB3(YDR169C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress Gene:TAT1(YBR069C)|FD-Score:6.09|P-value:5.71E-10||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA6(YPR157W_p)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:TPK2(YPL203W)|FD-Score:-4.45|P-value:4.37E-6||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TRM12(YML005W)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA Gene:TRP3(YKL211C)|FD-Score:5.17|P-value:1.18E-7||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSR2(YLR435W)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:TUB3(YML124C)|FD-Score:6.21|P-value:2.61E-10||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBC7(YMR022W)|FD-Score:6.4|P-value:7.93E-11||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:VID22(YLR373C)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:WSS1(YHR134W)|FD-Score:4.43|P-value:4.72E-6||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YBL029C-A(YBL029C-A_p)|FD-Score:-4.33|P-value:7.60E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica Gene:YBL071C-B(YBL071C-B_p)|FD-Score:5.61|P-value:9.93E-9||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR277C(YBR277C_d)|FD-Score:5.2|P-value:9.70E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCR102C(YCR102C_p)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDL118W(YDL118W_p)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR008C(YDR008C_d)|FD-Score:5.16|P-value:1.26E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR201C(YGR201C_p)|FD-Score:-3.15|P-value:8.14E-4||SGD DESC:Putative protein of unknown function Gene:YHR022C-A(YHR022C-A_p)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YIL054W(YIL054W_p)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YIR035C(YIR035C_p)|FD-Score:-3.13|P-value:8.67E-4||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YJL135W(YJL135W_d)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3 Gene:YJR151W-A(YJR151W-A_p)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational guilt by association analysis Gene:YKR045C(YKR045C_p)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YMR031W-A(YMR031W-A_d)|FD-Score:4.08|P-value:2.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR196W(YMR196W_p)|FD-Score:3.75|P-value:8.98E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YOL073C(YOL073C_p)|FD-Score:3.98|P-value:3.45E-5||SGD DESC:Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2 Gene:YOR338W(YOR338W_p)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication Gene:YPL080C(YPL080C_d)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR084W(YPR084W_p)|FD-Score:-4.68|P-value:1.43E-6||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR165W16.205.40E-5911.50RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YPR103W4.681.46E-60.11PRE2Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
YBL105C4.572.49E-60.26PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YBR202W4.318.32E-60.02MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YDL102W4.289.15E-60.09POL3Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)
YJL125C4.191.40E-50.05GCD14Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YIL118W4.141.76E-50.69RHO3Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p
YPR181C3.452.82E-40.07SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
YNR026C3.383.66E-40.01SEC12Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER
YPL251W_d3.363.86E-40.21YPL251W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
YDR339C3.167.98E-40.04FCF1Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p
YFR051C3.129.00E-40.02RET2Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
YHR143W-A3.109.64E-40.01RPC10RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress
YKL141W3.099.99E-40.25SDH3Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex
YOL123W2.840.002280.05HRP1Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR106W15.107.24E-52PHO88Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations
YMR015C7.871.84E-15ERG5C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs
YDL020C7.473.97E-14RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YPL236C7.049.83E-13ENV7Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16)
YMR022W6.407.93E-11UBC7Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
YML124C6.212.61E-10TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YDR127W6.173.44E-10ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YBR069C6.095.71E-10TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YHR076W5.686.70E-9PTC7Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope
YBL071C-B_p5.619.93E-9YBL071C-B_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YNL148C5.432.78E-8ALF1Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
YBR277C_d5.209.70E-8YBR277C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W
YKL211C5.171.18E-7TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YJR073C5.161.21E-7OPI3Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis
YDR008C_d5.161.26E-7YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_450
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2613.08E-92SGTC_9052-aminofluorene 215.0 μMChemDiv (Drug-like library)15390.162162cell wall signaling
0.2111.92E-60SGTC_32799140807 49.5 μMChembridge (Drug-like library)455957180.116279
0.2081.36E-58SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.0701754
0.2022.92E-55SGTC_6420335-0876 158.0 μMChemDiv (Drug-like library)68123500.05ubiquinone biosynthesis & proteasome
0.1999.59E-54SGTC_32859112818 20.9 μMChembridge (Drug-like library)170179470.0615385cell wall
0.1906.27E-49SGTC_8370091-0265 12.5 μMChemDiv (Drug-like library)7593670.147059ubiquinone biosynthesis & proteasome
0.1886.14E-48SGTC_483fpl-64176 144.0 μMICCB bioactive library34230.0727273cell wall
0.1863.52E-47SGTC_10533366-3204 134.0 μMChemDiv (Drug-like library)36320710.0576923cell wall
0.1782.12E-43SGTC_33319141060 2.0 μMChembridge (Drug-like library)49065670.0615385cell wall
0.1761.86E-42SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0357143
0.1755.01E-42SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.0266667
0.1741.87E-41SGTC_2232chlorophetanol 41.5 μMMiscellaneous159070.0789474TRP & mitochondrial translation
0.1741.87E-41SGTC_15101123-0200 71.5 μMChemDiv (Drug-like library)53788120.0869565
0.1723.18E-40SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.0888889
0.1674.75E-38SGTC_1874staurosporine 7.6 μMICCB bioactive library468641930.0789474cell wall signaling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_546peri-Naphthalide147 μM0.243243223824ChemDiv (Drug-like library)184.190722.34702DNA damage response
SGTC_1070358-00222.69 μM0.235294521106ChemDiv (Drug-like library)350.453965.87124SWF1 & branched chain AA biosynthesis
SGTC_9741326-0144138 μM0.2307695411856ChemDiv (Drug-like library)198.22061.96612ERG2
SGTC_12720839-002127 μM0.2255027250ChemDiv (Drug-like library)210.2282.46802
SGTC_20305175110200 μM0.2252831986Chembridge (Fragment library)188.182721.20523Golgi
SGTC_11423253-14089.78 μM0.219512256655ChemDiv (Drug-like library)276.332443.94123
SGTC_6690108-002126.2 μM0.21212117201ChemDiv (Drug-like library)292.331843.45724fatty acid desaturase (OLE1)
SGTC_23719071574200 μM0.20588245596039Chembridge (Fragment library)203.732162.73322
SGTC_23739071805200 μM0.20588210149009Chembridge (Fragment library)153.244641.97312
SGTC_20295175081200 μM0.2051281380876Chembridge (Fragment library)174.19922.12223