gingerol

(5S)-5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)decan-3-one

A herb.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_456
Screen concentration 170.0 μM
Source ICCB bioactive library
PubChem CID 442793
SMILES CCCCCC(CC(=O)CCC1=CC(=C(C=C1)O)OC)O
Standardized SMILES CCCCCC(O)CC(=O)CCc1ccc(O)c(OC)c1
Molecular weight 294.3859
ALogP 3.64
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.99
% growth inhibition (Hom. pool) 10.69


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 442793
Download HIP data (tab-delimited text)  (excel)
Gene:DAD2(YKR083C)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.01||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:GPI13(YLL031C)|FD-Score:5.86|P-value:2.29E-9|Clearance:1.35||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:MED4(YOR174W)|FD-Score:4.22|P-value:1.21E-5|Clearance:0.51||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MTR3(YGR158C)|FD-Score:3.15|P-value:8.26E-4|Clearance:0.09||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:RPP1(YHR062C)|FD-Score:7.81|P-value:2.83E-15|Clearance:1.95||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS3(YNL178W)|FD-Score:3.46|P-value:2.74E-4|Clearance:0.31||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP9(YPR137W)|FD-Score:4.51|P-value:3.19E-6|Clearance:0.29||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SEC65(YML105C)|FD-Score:-5.33|P-value:4.84E-8|Clearance:0||SGD DESC:Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 Gene:SMC2(YFR031C)|FD-Score:3.7|P-value:1.07E-4|Clearance:0.21||SGD DESC:Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus Gene:YLR076C(YLR076C_d)|FD-Score:3.49|P-value:2.38E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:DAD2(YKR083C)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.01||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:GPI13(YLL031C)|FD-Score:5.86|P-value:2.29E-9|Clearance:1.35||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:MED4(YOR174W)|FD-Score:4.22|P-value:1.21E-5|Clearance:0.51||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MTR3(YGR158C)|FD-Score:3.15|P-value:8.26E-4|Clearance:0.09||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:RPP1(YHR062C)|FD-Score:7.81|P-value:2.83E-15|Clearance:1.95||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS3(YNL178W)|FD-Score:3.46|P-value:2.74E-4|Clearance:0.31||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP9(YPR137W)|FD-Score:4.51|P-value:3.19E-6|Clearance:0.29||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SEC65(YML105C)|FD-Score:-5.33|P-value:4.84E-8|Clearance:0||SGD DESC:Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 Gene:SMC2(YFR031C)|FD-Score:3.7|P-value:1.07E-4|Clearance:0.21||SGD DESC:Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus Gene:YLR076C(YLR076C_d)|FD-Score:3.49|P-value:2.38E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 442793
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AIM5(YBR262C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:ALD2(YMR170C)|FD-Score:5.2|P-value:9.99E-8||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:ARI1(YGL157W)|FD-Score:3.77|P-value:8.27E-5||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:ARO1(YDR127W)|FD-Score:6.79|P-value:5.80E-12||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARP1(YHR129C)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:ATG7(YHR171W)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:CCM1(YGR150C)|FD-Score:3.89|P-value:4.98E-5||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CDA2(YLR308W)|FD-Score:-3.76|P-value:8.46E-5||SGD DESC:Chitin deacetylase, together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:CLB3(YDL155W)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress Gene:COX10(YPL172C)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:CYB5(YNL111C)|FD-Score:-3.2|P-value:6.83E-4||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DAL7(YIR031C)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:FKH2(YNL068C)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:FMP16(YDR070C_p)|FD-Score:-3.28|P-value:5.23E-4||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:FRE4(YNR060W)|FD-Score:3.75|P-value:8.88E-5||SGD DESC:Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:GCN2(YDR283C)|FD-Score:3.88|P-value:5.21E-5||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GIM5(YML094W)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:GSY1(YFR015C)|FD-Score:6.89|P-value:2.73E-12||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:HIS5(YIL116W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:IKI1(YHR187W)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:IME4(YGL192W)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:JNM1(YMR294W)|FD-Score:6.03|P-value:8.18E-10||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:KAP120(YPL125W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:KAP123(YER110C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:LDB18(YLL049W)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Component of the dynactin complex, which is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24 Gene:LYP1(YNL268W)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:LYS21(YDL131W)|FD-Score:-3.86|P-value:5.78E-5||SGD DESC:Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication Gene:MET31(YPL038W)|FD-Score:4.52|P-value:3.03E-6||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET31 has a paralog, MET32, that arose from the whole genome duplication Gene:MGR2(YPL098C)|FD-Score:-4.4|P-value:5.43E-6||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MLH1(YMR167W)|FD-Score:4.31|P-value:8.25E-6||SGD DESC:Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer Gene:MRE11(YMR224C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MRN1(YPL184C)|FD-Score:3.81|P-value:6.98E-5||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:MRP1(YDR347W)|FD-Score:3.88|P-value:5.23E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein Gene:MRP21(YBL090W)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL32(YCR003W)|FD-Score:4.9|P-value:4.82E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MSC2(YDR205W)|FD-Score:-3.2|P-value:6.76E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:MSF1(YPR047W)|FD-Score:4.27|P-value:9.81E-6||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:NUM1(YDR150W)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:NUR1(YDL089W)|FD-Score:-3.74|P-value:9.08E-5||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:PDR10(YOR328W)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in the pleiotropic drug resistance network; regulated by Pdr1p and Pdr3p Gene:PET54(YGR222W)|FD-Score:3.72|P-value:9.88E-5||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PGC1(YPL206C)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:PMT2(YAL023C)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PUS2(YGL063W)|FD-Score:-3.99|P-value:3.29E-5||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:PUT2(YHR037W)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:RFS1(YBR052C)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication Gene:SIM1(YIL123W)|FD-Score:4.35|P-value:6.83E-6||SGD DESC:Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated Gene:SRN2(YLR119W)|FD-Score:-3.11|P-value:9.31E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:SST2(YLR452C)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family Gene:VBA3(YCL069W)|FD-Score:5.94|P-value:1.40E-9||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS70(YJR126C)|FD-Score:3.83|P-value:6.47E-5||SGD DESC:Protein of unknown function involved in vacuolar protein sorting Gene:WSS1(YHR134W)|FD-Score:4.61|P-value:1.99E-6||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YAR035C-A(YAR035C-A_p)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YBL029C-A(YBL029C-A_p)|FD-Score:4.5|P-value:3.41E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica Gene:YBR277C(YBR277C_d)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCL042W(YCL042W_p)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YDR239C(YDR239C)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YDR274C(YDR274C_d)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL008W(YEL008W_d)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Hypothetical protein predicted to be involved in metabolism Gene:YGL138C(YGL138C_p)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YGR107W(YGR107W_d)|FD-Score:5.27|P-value:6.73E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL067C(YIL067C_p)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Uncharacterized protein of unknown function Gene:YIL141W(YIL141W_d)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIR035C(YIR035C_p)|FD-Score:-3.15|P-value:8.14E-4||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YJL218W(YJL218W_p)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene Gene:YKR070W(YKR070W_p)|FD-Score:-3.94|P-value:4.09E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR455W(YLR455W_p)|FD-Score:-4.01|P-value:3.07E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YML012C-A(YML012C-A_d)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR254C(YMR254C_d)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR123C(YPR123C_d)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:ACE2(YLR131C)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AIM5(YBR262C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:ALD2(YMR170C)|FD-Score:5.2|P-value:9.99E-8||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:ARI1(YGL157W)|FD-Score:3.77|P-value:8.27E-5||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:ARO1(YDR127W)|FD-Score:6.79|P-value:5.80E-12||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARP1(YHR129C)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:ATG7(YHR171W)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:CCM1(YGR150C)|FD-Score:3.89|P-value:4.98E-5||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CDA2(YLR308W)|FD-Score:-3.76|P-value:8.46E-5||SGD DESC:Chitin deacetylase, together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:CLB3(YDL155W)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress Gene:COX10(YPL172C)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:CYB5(YNL111C)|FD-Score:-3.2|P-value:6.83E-4||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DAL7(YIR031C)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:FKH2(YNL068C)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:FMP16(YDR070C_p)|FD-Score:-3.28|P-value:5.23E-4||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:FRE4(YNR060W)|FD-Score:3.75|P-value:8.88E-5||SGD DESC:Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:GCN2(YDR283C)|FD-Score:3.88|P-value:5.21E-5||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GIM5(YML094W)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:GSY1(YFR015C)|FD-Score:6.89|P-value:2.73E-12||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:HIS5(YIL116W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:IKI1(YHR187W)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:IME4(YGL192W)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:JNM1(YMR294W)|FD-Score:6.03|P-value:8.18E-10||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:KAP120(YPL125W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:KAP123(YER110C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:LDB18(YLL049W)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Component of the dynactin complex, which is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24 Gene:LYP1(YNL268W)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:LYS21(YDL131W)|FD-Score:-3.86|P-value:5.78E-5||SGD DESC:Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication Gene:MET31(YPL038W)|FD-Score:4.52|P-value:3.03E-6||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET31 has a paralog, MET32, that arose from the whole genome duplication Gene:MGR2(YPL098C)|FD-Score:-4.4|P-value:5.43E-6||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MLH1(YMR167W)|FD-Score:4.31|P-value:8.25E-6||SGD DESC:Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer Gene:MRE11(YMR224C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MRN1(YPL184C)|FD-Score:3.81|P-value:6.98E-5||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:MRP1(YDR347W)|FD-Score:3.88|P-value:5.23E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein Gene:MRP21(YBL090W)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL32(YCR003W)|FD-Score:4.9|P-value:4.82E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MSC2(YDR205W)|FD-Score:-3.2|P-value:6.76E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:MSF1(YPR047W)|FD-Score:4.27|P-value:9.81E-6||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:NUM1(YDR150W)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:NUR1(YDL089W)|FD-Score:-3.74|P-value:9.08E-5||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:PDR10(YOR328W)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in the pleiotropic drug resistance network; regulated by Pdr1p and Pdr3p Gene:PET54(YGR222W)|FD-Score:3.72|P-value:9.88E-5||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PGC1(YPL206C)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:PMT2(YAL023C)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PUS2(YGL063W)|FD-Score:-3.99|P-value:3.29E-5||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:PUT2(YHR037W)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:RFS1(YBR052C)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication Gene:SIM1(YIL123W)|FD-Score:4.35|P-value:6.83E-6||SGD DESC:Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated Gene:SRN2(YLR119W)|FD-Score:-3.11|P-value:9.31E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:SST2(YLR452C)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family Gene:VBA3(YCL069W)|FD-Score:5.94|P-value:1.40E-9||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS70(YJR126C)|FD-Score:3.83|P-value:6.47E-5||SGD DESC:Protein of unknown function involved in vacuolar protein sorting Gene:WSS1(YHR134W)|FD-Score:4.61|P-value:1.99E-6||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YAR035C-A(YAR035C-A_p)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YBL029C-A(YBL029C-A_p)|FD-Score:4.5|P-value:3.41E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica Gene:YBR277C(YBR277C_d)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCL042W(YCL042W_p)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YDR239C(YDR239C)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YDR274C(YDR274C_d)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL008W(YEL008W_d)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Hypothetical protein predicted to be involved in metabolism Gene:YGL138C(YGL138C_p)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YGR107W(YGR107W_d)|FD-Score:5.27|P-value:6.73E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL067C(YIL067C_p)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Uncharacterized protein of unknown function Gene:YIL141W(YIL141W_d)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIR035C(YIR035C_p)|FD-Score:-3.15|P-value:8.14E-4||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YJL218W(YJL218W_p)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene Gene:YKR070W(YKR070W_p)|FD-Score:-3.94|P-value:4.09E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR455W(YLR455W_p)|FD-Score:-4.01|P-value:3.07E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YML012C-A(YML012C-A_d)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR254C(YMR254C_d)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR123C(YPR123C_d)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C7.812.83E-151.95RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YLL031C5.862.29E-91.35GPI13ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein
YPR137W4.513.19E-60.29RRP9Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein
YOR174W4.221.21E-50.51MED4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YKR083C3.711.02E-40.01DAD2Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YFR031C3.701.07E-40.21SMC2Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus
YLR076C_d3.492.38E-40.04YLR076C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10
YNL178W3.462.74E-40.31RPS3Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3
YGR158C3.158.26E-40.09MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
YLR002C3.060.001110.17NOC3Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing
YOR181W2.880.001970.04LAS17Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP)
YOR146W_d2.840.002250.19YOR146W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
YGR186W2.650.004000.04TFG1TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74
YDR166C2.610.004490.08SEC5Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
YDL217C2.540.005600.00TIM22Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YFR015C6.892.73E-12GSY1Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YDR127W6.795.80E-12ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YMR294W6.038.18E-10JNM1Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B
YCL069W5.941.40E-9VBA3Permease of basic amino acids in the vacuolar membrane
YGR107W_d5.276.73E-8YGR107W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YMR170C5.209.99E-8ALD2Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p
YCR003W4.904.82E-7MRPL32Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
YHR134W4.611.99E-6WSS1Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response
YPL038W4.523.03E-6MET31Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET31 has a paralog, MET32, that arose from the whole genome duplication
YBL029C-A_p4.503.41E-6YBL029C-A_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica
YIL123W4.356.83E-6SIM1Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated
YMR167W4.318.25E-6MLH1Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer
YPR047W4.279.81E-6MSF1Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase
YIL067C_p4.261.04E-5YIL067C_pUncharacterized protein of unknown function
YPL206C4.251.08E-5PGC1Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs

GO enrichment analysis for SGTC_456
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1301.30E-23SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.0412371
0.1224.90E-21SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0649351
0.1132.44E-18SGTC_18815652484 16.0 μMMiscellaneous22551290.0813954TRP & mitochondrial translation
0.1133.77E-18SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.0588235TRP & mitochondrial translation
0.1095.35E-17SGTC_20245144227 134.0 μMChembridge (Fragment library)34752110.0958904
0.1057.92E-16SGTC_6390833-0042 114.0 μMChemDiv (Drug-like library)59789110.0958904
0.1041.14E-15SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.0595238RPP1 & pyrimidine depletion
0.1041.43E-15SGTC_5810958-0271 211.0 μMChemDiv (Drug-like library)45911450.139241
0.1016.51E-15SGTC_15346-azauridine 81.6 μMTimTec (Pure natural product library)2335020.0666667RNA pol III & RNase P/MRP
0.1001.44E-14SGTC_442cape 100.0 μMICCB bioactive library52817870.223881
0.0992.95E-14SGTC_265mycophenolic acid 32.9 μMMiscellaneous4465410.15RPP1 & pyrimidine depletion
0.0983.90E-14SGTC_20865274770 176.6 μMChembridge (Fragment library)6892780.0447761
0.0953.27E-13SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.149254
0.0943.51E-13SGTC_18825615643 20.0 μMMiscellaneous22530750.0804598TRP & mitochondrial translation
0.0937.75E-13SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.0675676

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1135dihydrocapsaicin536.71 nM0.474576107982TimTec (Natural product library)307.427764.35423
SGTC_1122capsaicin818.57 nM0.41548943TimTec (Natural product library)305.411883.9123
SGTC_2684capsaicin100 μM0.41548943Miscellaneous305.411883.9123
SGTC_2669eugenol100 μM0.3846153314Microsource (Natural product library)164.201082.57912
SGTC_2614arthonioic acid13.39 μM0.3239446710684Microsource (Natural product library)528.590745.99239
SGTC_790052-000794.09 μM0.3103452801472ChemDiv (Drug-like library)276.370664.63813
SGTC_1925st05777010.1 μM0.3015875989122TimTec (Natural product derivative library)284.306543.68724TSC3-RPN4
SGTC_820054-0107293.76 μM0.2903233104697ChemDiv (Drug-like library)340.412866.11514
SGTC_9591222-0044107 μM0.2881366083612ChemDiv (Drug-like library)232.31814.68512
SGTC_441propafenone146 μM0.2857144932Miscellaneous341.443983.67324
SGTC_9581222-000733 μM0.2833337312461ChemDiv (Drug-like library)246.344685.14112