nppb

5-nitro-2-(3-phenylpropylamino)benzoic acid

A chloride channel blocker.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 

PubChem MeSH terms: Angiogenesis Inhibitors



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_458
Screen concentration 133.0 μM
Source ICCB bioactive library
PubChem CID 4549
SMILES C1=CC=C(C=C1)CCCNC2=C(C=C(C=C2)[N+](=O)[O-])C(=O)O
Standardized SMILES OC(=O)c1cc(ccc1NCCCc2ccccc2)[N+](=O)[O-]
Molecular weight 300.3092
ALogP 3.52
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.43
% growth inhibition (Hom. pool) 5.2


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4549
Download HIP data (tab-delimited text)  (excel)
Gene:CCT5(YJR064W)|FD-Score:3.14|P-value:8.31E-4|Clearance:0.13||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:GCD14(YJL125C)|FD-Score:4.5|P-value:3.37E-6|Clearance:1.33||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:POP8(YBL018C)|FD-Score:3.18|P-value:7.49E-4|Clearance:0.03||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPC53(YDL150W)|FD-Score:-3.63|P-value:1.39E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:SNU23(YDL098C)|FD-Score:-3.27|P-value:5.31E-4|Clearance:0||SGD DESC:Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome Gene:CCT5(YJR064W)|FD-Score:3.14|P-value:8.31E-4|Clearance:0.13||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:GCD14(YJL125C)|FD-Score:4.5|P-value:3.37E-6|Clearance:1.33||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:POP8(YBL018C)|FD-Score:3.18|P-value:7.49E-4|Clearance:0.03||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPC53(YDL150W)|FD-Score:-3.63|P-value:1.39E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:SNU23(YDL098C)|FD-Score:-3.27|P-value:5.31E-4|Clearance:0||SGD DESC:Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4549
Download HOP data (tab-delimited text)  (excel)
Gene:AHP1(YLR109W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p Gene:AIM29(YKR074W)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:APD1(YBR151W)|FD-Score:-3.13|P-value:8.72E-4||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:ARC18(YLR370C)|FD-Score:3.84|P-value:6.10E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARO9(YHR137W)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism Gene:ATG21(YPL100W)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein Gene:BEM4(YPL161C)|FD-Score:4.46|P-value:4.12E-6||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:DHH1(YDL160C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:DYN2(YDR424C)|FD-Score:4.63|P-value:1.83E-6||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:ENV7(YPL236C)|FD-Score:5.29|P-value:6.00E-8||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:ENV9(YOR246C)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:GEP3(YOR205C)|FD-Score:3.74|P-value:9.07E-5||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GGC1(YDL198C)|FD-Score:3.74|P-value:9.38E-5||SGD DESC:Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family Gene:HFI1(YPL254W)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:ISR1(YPR106W)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:LEM3(YNL323W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MAC1(YMR021C)|FD-Score:4.93|P-value:4.18E-7||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MSC2(YDR205W)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:NUP60(YAR002W)|FD-Score:4.42|P-value:4.98E-6||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:PER1(YCR044C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PEX7(YDR142C)|FD-Score:-3.18|P-value:7.46E-4||SGD DESC:Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) Gene:PST2(YDR032C)|FD-Score:-4.65|P-value:1.70E-6||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:RAD27(YKL113C)|FD-Score:4.74|P-value:1.07E-6||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RCK1(YGL158W)|FD-Score:-4.19|P-value:1.41E-5||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:ROX3(YBL093C)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL23B(YER117W)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:4.32|P-value:7.84E-6||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDP1(YIL113W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SMF3(YLR034C)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:SOD1(YJR104C)|FD-Score:6.22|P-value:2.48E-10||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SRX1(YKL086W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress Gene:TCB1(YOR086C)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TRP4(YDR354W)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VCX1(YDL128W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity, involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter Gene:VMA9(YCL005W-A)|FD-Score:-3.09|P-value:9.96E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:YBR277C(YBR277C_d)|FD-Score:3.2|P-value:6.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YDR286C(YDR286C_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site Gene:YFR012W-A(YFR012W-A_p)|FD-Score:5.56|P-value:1.36E-8||SGD DESC:Putative protein of unknown function; identified by homology Gene:YHR177W(YHR177W_p)|FD-Score:3.75|P-value:8.86E-5||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YJR039W(YJR039W_p)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNL046W(YNL046W_p)|FD-Score:5.9|P-value:1.83E-9||SGD DESC:Putative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YNL296W(YNL296W_d)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Gene:AHP1(YLR109W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p Gene:AIM29(YKR074W)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:APD1(YBR151W)|FD-Score:-3.13|P-value:8.72E-4||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:ARC18(YLR370C)|FD-Score:3.84|P-value:6.10E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARO9(YHR137W)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism Gene:ATG21(YPL100W)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein Gene:BEM4(YPL161C)|FD-Score:4.46|P-value:4.12E-6||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:DHH1(YDL160C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:DYN2(YDR424C)|FD-Score:4.63|P-value:1.83E-6||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:ENV7(YPL236C)|FD-Score:5.29|P-value:6.00E-8||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:ENV9(YOR246C)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:GEP3(YOR205C)|FD-Score:3.74|P-value:9.07E-5||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GGC1(YDL198C)|FD-Score:3.74|P-value:9.38E-5||SGD DESC:Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family Gene:HFI1(YPL254W)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:ISR1(YPR106W)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:LEM3(YNL323W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MAC1(YMR021C)|FD-Score:4.93|P-value:4.18E-7||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MSC2(YDR205W)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:NUP60(YAR002W)|FD-Score:4.42|P-value:4.98E-6||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:PER1(YCR044C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PEX7(YDR142C)|FD-Score:-3.18|P-value:7.46E-4||SGD DESC:Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) Gene:PST2(YDR032C)|FD-Score:-4.65|P-value:1.70E-6||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:RAD27(YKL113C)|FD-Score:4.74|P-value:1.07E-6||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RCK1(YGL158W)|FD-Score:-4.19|P-value:1.41E-5||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:ROX3(YBL093C)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL23B(YER117W)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:4.32|P-value:7.84E-6||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDP1(YIL113W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SMF3(YLR034C)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:SOD1(YJR104C)|FD-Score:6.22|P-value:2.48E-10||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SRX1(YKL086W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress Gene:TCB1(YOR086C)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TRP4(YDR354W)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VCX1(YDL128W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity, involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter Gene:VMA9(YCL005W-A)|FD-Score:-3.09|P-value:9.96E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:YBR277C(YBR277C_d)|FD-Score:3.2|P-value:6.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YDR286C(YDR286C_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site Gene:YFR012W-A(YFR012W-A_p)|FD-Score:5.56|P-value:1.36E-8||SGD DESC:Putative protein of unknown function; identified by homology Gene:YHR177W(YHR177W_p)|FD-Score:3.75|P-value:8.86E-5||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YJR039W(YJR039W_p)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNL046W(YNL046W_p)|FD-Score:5.9|P-value:1.83E-9||SGD DESC:Putative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YNL296W(YNL296W_d)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL125C4.503.37E-61.33GCD14Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YBL018C3.187.49E-40.03POP8Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YJR064W3.148.31E-40.13CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YPL231W3.010.001300.11FAS2Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities
YHR020W2.900.001860.02YHR020WProlyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments
YIL062C2.890.001950.13ARC15Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity
YHR190W2.760.002920.00ERG9Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway
YDR160W2.760.002930.07SSY1Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YGR267C2.680.003650.09FOL2GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway
YIR006C2.590.004810.13PAN1Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease
YER133W2.460.006920.04GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YDR464W2.420.007770.01SPP41Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3
YNR035C2.410.008040.10ARC35Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin
YLR317W_d2.310.010400.03YLR317W_dDubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C
YHR188C2.280.011200.12GPI16Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJR104C6.222.48E-10SOD1Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
YNL046W_p5.901.83E-9YNL046W_pPutative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
YFR012W-A_p5.561.36E-8YFR012W-A_pPutative protein of unknown function; identified by homology
YPL236C5.296.00E-8ENV7Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16)
YMR021C4.934.18E-7MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YKL113C4.741.07E-6RAD275' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family
YDR424C4.631.83E-6DYN2Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex
YPL161C4.464.12E-6BEM4Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length
YAR002W4.424.98E-6NUP60FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153
YDR129C4.327.84E-6SAC6Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
YBL093C4.241.14E-5ROX3Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme
YKR074W4.211.29E-5AIM29Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss
YPL254W3.855.95E-5HFI1Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions
YLR370C3.846.10E-5ARC18Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YHR177W_p3.758.86E-5YHR177W_pPutative protein of unknown function; overexpression causes a cell cycle delay or arrest

GO enrichment analysis for SGTC_458
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1226.01E-21SGTC_2741miltefosine 3.0 μMMiscellaneous35990.0606061
0.1111.66E-17SGTC_20805212524 12.4 μMChembridge (Fragment library)2601830.118644copper-dependent oxidative stress
0.1031.58E-15SGTC_22667929956 200.0 μMChembridge (Fragment library)29671530.115942
0.0936.27E-13SGTC_21715739187 198.1 μMChembridge (Fragment library)22627610.127273copper-dependent oxidative stress
0.0903.74E-12SGTC_31199125183 49.5 μMChembridge (Drug-like library)226953340.0857143
0.0895.86E-12SGTC_21896047310 154.4 μMChembridge (Fragment library)7115350.0833333RPP1 & pyrimidine depletion
0.0881.04E-11SGTC_2507purpurin 79.7 μMMicrosource (Natural product library)66830.137931copper-dependent oxidative stress
0.0863.66E-11SGTC_4291112-0049 25.5 μMChemDiv (Drug-like library)28361700.0789474DNA damage response
0.0822.62E-10SGTC_12670828-0272 9.5 μMChemDiv (Drug-like library)7453340.101449
0.0815.77E-10SGTC_10583448-5425 97.3 μMChemDiv (Drug-like library)72797050.104478
0.0808.51E-10SGTC_740k014-0057 261.0 μMChemDiv (Drug-like library)40127790.225
0.0764.26E-9SGTC_21966573537 42.7 μMChembridge (Fragment library)29030870.125copper-dependent oxidative stress
0.0765.14E-9SGTC_11841269-1718 139.0 μMChemDiv (Drug-like library)54038210.111111
0.0757.97E-9SGTC_10004048-0884 8.7 μMChemDiv (Drug-like library)130400880.205882copper-dependent oxidative stress
0.0741.04E-8SGTC_2682pyrogallin 40.0 μMMicrosource (Natural product library)8227980.078125copper-dependent oxidative stress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_850868-0259160.06 μM0.39622683173ChemDiv (Drug-like library)256.256682.5431360S ribosome export
SGTC_410478-063282.64 μM0.3773583750424ChemDiv (Drug-like library)229.234642.52724
SGTC_260302-0163425.16 μM0.3636363100915ChemDiv (Drug-like library)275.216962.92626heme biosynthesis & mitochondrial translocase
SGTC_13281441-002569.2 μM0.3620694562601ChemDiv (Drug-like library)287.270722.2724
SGTC_8890919-152482.7 μM0.3606563905452ChemDiv (Drug-like library)274.271962.85825
SGTC_225nsc-1226571.02 μM0.357143275685Miscellaneous322.29332.72806
SGTC_9262268-140159.4 μM0.3492062931578ChemDiv (Drug-like library)390.388724.27926TSC3-RPN4
SGTC_797k781-0471270 μM0.34426223738129ChemDiv (Drug-like library)306.701143.4125RPP1 & pyrimidine depletion
SGTC_507nimesulide162 μM0.3333334495Miscellaneous308.309782.19516
SGTC_6260214-000933 μM0.3275863437874ChemDiv (Drug-like library)332.225222.29717copper-dependent oxidative stress