bw-b 70c

1-[(E)-4-[3-(4-fluorophenoxy)phenyl]but-3-en-2-yl]-1-hydroxyurea

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_464
Screen concentration 158.0 μM
Source ICCB bioactive library
PubChem CID 5353454
SMILES CC(C=CC1=CC(=CC=C1)OC2=CC=C(C=C2)F)N(C(=O)N)O
Standardized SMILES CC(C=Cc1cccc(Oc2ccc(F)cc2)c1)N(O)C(=O)N
Molecular weight 316.3269
ALogP 3.37
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.54
% growth inhibition (Hom. pool) 5.59


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5353454
Download HIP data (tab-delimited text)  (excel)
Gene:ARC40(YBR234C)|FD-Score:3.76|P-value:8.43E-5|Clearance:0.06||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CCT3(YJL014W)|FD-Score:5.66|P-value:7.51E-9|Clearance:1.3||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.2||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CWC2(YDL209C)|FD-Score:6.24|P-value:2.16E-10|Clearance:1.3||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:DBP10(YDL031W)|FD-Score:3.5|P-value:2.33E-4|Clearance:0.4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis Gene:GLC7(YER133W)|FD-Score:5.58|P-value:1.23E-8|Clearance:1.3||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:NOG1(YPL093W)|FD-Score:4.14|P-value:1.76E-5|Clearance:0.29||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:POP5(YAL033W)|FD-Score:3.85|P-value:6.01E-5|Clearance:0.08||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPP1(YHR062C)|FD-Score:4.24|P-value:1.09E-5|Clearance:0.02||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT5(YOR117W)|FD-Score:-4.35|P-value:6.89E-6|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:SEC16(YPL085W)|FD-Score:5.54|P-value:1.47E-8|Clearance:1.3||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:SEC39(YLR440C)|FD-Score:4.22|P-value:1.20E-5|Clearance:0.09||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SRP14(YDL092W)|FD-Score:3.1|P-value:9.53E-4|Clearance:0.03||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 Gene:ARC40(YBR234C)|FD-Score:3.76|P-value:8.43E-5|Clearance:0.06||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CCT3(YJL014W)|FD-Score:5.66|P-value:7.51E-9|Clearance:1.3||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.2||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CWC2(YDL209C)|FD-Score:6.24|P-value:2.16E-10|Clearance:1.3||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:DBP10(YDL031W)|FD-Score:3.5|P-value:2.33E-4|Clearance:0.4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis Gene:GLC7(YER133W)|FD-Score:5.58|P-value:1.23E-8|Clearance:1.3||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:NOG1(YPL093W)|FD-Score:4.14|P-value:1.76E-5|Clearance:0.29||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:POP5(YAL033W)|FD-Score:3.85|P-value:6.01E-5|Clearance:0.08||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPP1(YHR062C)|FD-Score:4.24|P-value:1.09E-5|Clearance:0.02||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT5(YOR117W)|FD-Score:-4.35|P-value:6.89E-6|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:SEC16(YPL085W)|FD-Score:5.54|P-value:1.47E-8|Clearance:1.3||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:SEC39(YLR440C)|FD-Score:4.22|P-value:1.20E-5|Clearance:0.09||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SRP14(YDL092W)|FD-Score:3.1|P-value:9.53E-4|Clearance:0.03||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5353454
Download HOP data (tab-delimited text)  (excel)
Gene:AGA2(YGL032C)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:ALG6(YOR002W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:APM1(YPL259C)|FD-Score:-4.35|P-value:6.68E-6||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:BCK1(YJL095W)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BGL2(YGR282C)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:BTS1(YPL069C)|FD-Score:-3.88|P-value:5.15E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD32(YGR262C)|FD-Score:3.96|P-value:3.80E-5||SGD DESC:Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:CAF20(YOR276W)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E Gene:CCC1(YLR220W)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to the vacuole decreases upon DNA replication stress Gene:CDC73(YLR418C)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CMR3(YPR013C_p)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:COA4(YLR218C)|FD-Score:4.38|P-value:5.99E-6||SGD DESC:Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors Gene:COQ10(YOL008W)|FD-Score:3.81|P-value:6.95E-5||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:COS1(YNL336W)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:DAL81(YIR023W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DOT1(YDR440W)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:ERG3(YLR056W)|FD-Score:-3.24|P-value:5.91E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FRA2(YGL220W)|FD-Score:-3.87|P-value:5.48E-5||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:GDS1(YOR355W)|FD-Score:3.32|P-value:4.42E-4||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GIS2(YNL255C)|FD-Score:-3.11|P-value:9.39E-4||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GUP1(YGL084C)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HIT1(YJR055W)|FD-Score:4.88|P-value:5.29E-7||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HOF1(YMR032W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p Gene:HOP2(YGL033W)|FD-Score:5.73|P-value:4.95E-9||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HXK1(YFR053C)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:IDP2(YLR174W)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication Gene:IRC21(YMR073C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:ISR1(YPR106W)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:IST1(YNL265C)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:JNM1(YMR294W)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LNP1(YHR192W_p)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MGR3(YMR115W)|FD-Score:3.9|P-value:4.79E-5||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MHF1(YOL086W-A)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 Gene:MMS22(YLR320W)|FD-Score:-4.4|P-value:5.42E-6||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MNN2(YBR015C)|FD-Score:7.66|P-value:9.05E-15||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRPS17(YMR188C)|FD-Score:3.74|P-value:9.24E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MTF1(YMR228W)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:MTG1(YMR097C)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NDJ1(YOL104C)|FD-Score:5.01|P-value:2.73E-7||SGD DESC:Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement Gene:NGG1(YDR176W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:ODC1(YPL134C)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation Gene:PEX19(YDL065C)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:PHO4(YFR034C)|FD-Score:3.82|P-value:6.60E-5||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PHO84(YML123C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PHO87(YCR037C)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PPA2(YMR267W)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:PTH1(YHR189W)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RCF2(YNR018W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast Gene:RPL20B(YOR312C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPL24A(YGL031C)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RPS23B(YPR132W)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RPS6A(YPL090C)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:RRI1(YDL216C)|FD-Score:-3.48|P-value:2.55E-4||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:SEH1(YGL100W)|FD-Score:3.92|P-value:4.47E-5||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SLA1(YBL007C)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLG1(YOR008C)|FD-Score:3.9|P-value:4.80E-5||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLM6(YBR266C_d)|FD-Score:3.1|P-value:9.84E-4||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SLT2(YHR030C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF2(YOR290C)|FD-Score:-5.44|P-value:2.60E-8||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:STP3(YLR375W)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:SWD3(YBR175W)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 Gene:TAT1(YBR069C)|FD-Score:4.96|P-value:3.60E-7||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:UBC13(YDR092W)|FD-Score:-3.55|P-value:1.89E-4||SGD DESC:Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus Gene:URA2(YJL130C)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:VMA1(YDL185W)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA8(YEL051W)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:VPH1(YOR270C)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:YAL056C-A(YAL056C-A_d)|FD-Score:4.79|P-value:8.18E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAR1(YPL239W)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YBL062W(YBL062W_d)|FD-Score:6.22|P-value:2.56E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCL042W(YCL042W_p)|FD-Score:-3.61|P-value:1.51E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YCR090C(YCR090C_p)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YDR161W(YDR161W_p)|FD-Score:5.66|P-value:7.42E-9||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YDR203W(YDR203W_d)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR524C-B(YDR524C-B_p)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YER039C-A(YER039C-A_p)|FD-Score:3.42|P-value:3.19E-4||SGD DESC:Putative protein of unknown function; YER039C-A is not an essential gene Gene:YGR069W(YGR069W_d)|FD-Score:-3.82|P-value:6.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR126W(YGR126W_p)|FD-Score:-3.12|P-value:9.11E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YHR182W(YHR182W_p)|FD-Score:-3.98|P-value:3.38E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YLR455W(YLR455W_p)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YNL050C(YNL050C_p)|FD-Score:4.58|P-value:2.37E-6||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YPL114W(YPL114W_d)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index Gene:YPR014C(YPR014C_d)|FD-Score:4.01|P-value:3.00E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPS7(YDR349C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum Gene:AGA2(YGL032C)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:ALG6(YOR002W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:APM1(YPL259C)|FD-Score:-4.35|P-value:6.68E-6||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:BCK1(YJL095W)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BGL2(YGR282C)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:BTS1(YPL069C)|FD-Score:-3.88|P-value:5.15E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD32(YGR262C)|FD-Score:3.96|P-value:3.80E-5||SGD DESC:Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:CAF20(YOR276W)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E Gene:CCC1(YLR220W)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to the vacuole decreases upon DNA replication stress Gene:CDC73(YLR418C)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CMR3(YPR013C_p)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:COA4(YLR218C)|FD-Score:4.38|P-value:5.99E-6||SGD DESC:Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors Gene:COQ10(YOL008W)|FD-Score:3.81|P-value:6.95E-5||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:COS1(YNL336W)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:DAL81(YIR023W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DOT1(YDR440W)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:ERG3(YLR056W)|FD-Score:-3.24|P-value:5.91E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FRA2(YGL220W)|FD-Score:-3.87|P-value:5.48E-5||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:GDS1(YOR355W)|FD-Score:3.32|P-value:4.42E-4||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GIS2(YNL255C)|FD-Score:-3.11|P-value:9.39E-4||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GUP1(YGL084C)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HIT1(YJR055W)|FD-Score:4.88|P-value:5.29E-7||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HOF1(YMR032W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p Gene:HOP2(YGL033W)|FD-Score:5.73|P-value:4.95E-9||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HXK1(YFR053C)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:IDP2(YLR174W)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication Gene:IRC21(YMR073C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:ISR1(YPR106W)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:IST1(YNL265C)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:JNM1(YMR294W)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LNP1(YHR192W_p)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MGR3(YMR115W)|FD-Score:3.9|P-value:4.79E-5||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MHF1(YOL086W-A)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 Gene:MMS22(YLR320W)|FD-Score:-4.4|P-value:5.42E-6||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MNN2(YBR015C)|FD-Score:7.66|P-value:9.05E-15||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRPS17(YMR188C)|FD-Score:3.74|P-value:9.24E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MTF1(YMR228W)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:MTG1(YMR097C)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NDJ1(YOL104C)|FD-Score:5.01|P-value:2.73E-7||SGD DESC:Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement Gene:NGG1(YDR176W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:ODC1(YPL134C)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation Gene:PEX19(YDL065C)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:PHO4(YFR034C)|FD-Score:3.82|P-value:6.60E-5||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PHO84(YML123C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PHO87(YCR037C)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PPA2(YMR267W)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:PTH1(YHR189W)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RCF2(YNR018W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast Gene:RPL20B(YOR312C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPL24A(YGL031C)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RPS23B(YPR132W)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RPS6A(YPL090C)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:RRI1(YDL216C)|FD-Score:-3.48|P-value:2.55E-4||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:SEH1(YGL100W)|FD-Score:3.92|P-value:4.47E-5||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SLA1(YBL007C)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLG1(YOR008C)|FD-Score:3.9|P-value:4.80E-5||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLM6(YBR266C_d)|FD-Score:3.1|P-value:9.84E-4||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SLT2(YHR030C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF2(YOR290C)|FD-Score:-5.44|P-value:2.60E-8||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:STP3(YLR375W)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:SWD3(YBR175W)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 Gene:TAT1(YBR069C)|FD-Score:4.96|P-value:3.60E-7||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:UBC13(YDR092W)|FD-Score:-3.55|P-value:1.89E-4||SGD DESC:Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus Gene:URA2(YJL130C)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:VMA1(YDL185W)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA8(YEL051W)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:VPH1(YOR270C)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:YAL056C-A(YAL056C-A_d)|FD-Score:4.79|P-value:8.18E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAR1(YPL239W)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YBL062W(YBL062W_d)|FD-Score:6.22|P-value:2.56E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCL042W(YCL042W_p)|FD-Score:-3.61|P-value:1.51E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YCR090C(YCR090C_p)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YDR161W(YDR161W_p)|FD-Score:5.66|P-value:7.42E-9||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YDR203W(YDR203W_d)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR524C-B(YDR524C-B_p)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YER039C-A(YER039C-A_p)|FD-Score:3.42|P-value:3.19E-4||SGD DESC:Putative protein of unknown function; YER039C-A is not an essential gene Gene:YGR069W(YGR069W_d)|FD-Score:-3.82|P-value:6.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR126W(YGR126W_p)|FD-Score:-3.12|P-value:9.11E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YHR182W(YHR182W_p)|FD-Score:-3.98|P-value:3.38E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YLR455W(YLR455W_p)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YNL050C(YNL050C_p)|FD-Score:4.58|P-value:2.37E-6||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YPL114W(YPL114W_d)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index Gene:YPR014C(YPR014C_d)|FD-Score:4.01|P-value:3.00E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPS7(YDR349C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL209C6.242.16E-101.30CWC2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
YJL014W5.667.51E-91.30CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YER133W5.581.23E-81.30GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YPL085W5.541.47E-81.30SEC16COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p
YHR062C4.241.09E-50.02RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YLR440C4.221.20E-50.09SEC39Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope
YPL093W4.141.76E-50.29NOG1Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
YAL033W3.856.01E-50.08POP5Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YBR234C3.768.43E-50.06ARC40Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YJL111W3.701.09E-40.20CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YDL031W3.502.33E-40.40DBP10Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis
YDL092W3.109.53E-40.03SRP14Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14
YDR449C3.080.001050.00UTP6Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YGL068W3.070.001060.02MNP1Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth
YPR016C3.060.001120.01TIF6Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR015C7.669.05E-15MNN2Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
YBL062W_d6.222.56E-10YBL062W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YGL033W5.734.95E-9HOP2Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair
YDR161W_p5.667.42E-9YDR161W_pPutative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD)
YOL104C5.012.73E-7NDJ1Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement
YBR069C4.963.60E-7TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YJR055W4.885.29E-7HIT1Protein of unknown function, required for growth at high temperature
YAL056C-A_d4.798.18E-7YAL056C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YCR037C4.651.69E-6PHO87Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication
YFR053C4.621.94E-6HXK1Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication
YNL050C_p4.582.37E-6YNL050C_pPutative protein of unknown function; YNL050c is not an essential gene
YOR270C4.532.93E-6VPH1Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress
YLR218C4.385.99E-6COA4Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors
YDR349C4.241.10E-5YPS7Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum
YEL051W4.171.53E-5VMA8Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis

GO enrichment analysis for SGTC_464
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1447.97E-29SGTC_500h-89 112.0 μMICCB bioactive library4492410.104651cell wall signaling
0.1093.60E-17SGTC_486niguldipine 82.0 μMMiscellaneous12360.11amide catabolism
0.1041.15E-15SGTC_20074011539 71.8 μMChembridge (Fragment library)455953010.084507cell wall signaling
0.1041.27E-15SGTC_422cantharidin 155.0 μMICCB bioactive library25450.047619cell wall signaling
0.1024.02E-15SGTC_1614st002383 48.6 μMTimTec (Natural product derivative library)19472350.154762cell wall signaling
0.1001.06E-14SGTC_32259130819 49.5 μMChembridge (Drug-like library)45471210.144737amide catabolism
0.0993.18E-14SGTC_408cantharidin 100.0 μMICCB bioactive library25450.047619cell wall signaling
0.0971.06E-13SGTC_6631486-0733 13.9 μMChemDiv (Drug-like library)7224450.0681818cell wall signaling
0.0961.17E-13SGTC_511blebbistatin 171.0 μMICCB bioactive library34769860.1DNA intercalators
0.0961.18E-13SGTC_8251988-2224 89.6 μMChemDiv (Drug-like library)11808590.121951cell wall signaling
0.0952.77E-13SGTC_29249010168 62.8 μMChembridge (Drug-like library)29939290.253731cell wall signaling
0.0943.56E-13SGTC_9052-aminofluorene 215.0 μMChemDiv (Drug-like library)15390.0967742cell wall signaling
0.0931.04E-12SGTC_6790422-0091 41.4 μMChemDiv (Drug-like library)6273840.0923077Golgi
0.0921.73E-12SGTC_2619obliquin 87.9 μMMicrosource (Natural product library)67085930.0779221cell wall
0.0912.44E-12SGTC_2415hr heat shock (37°C) + cantharidin 155.4 μMICCB bioactive library25450.047619cell wall signaling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_155k072-020243.6 μM0.3230775857737ChemDiv (Drug-like library)332.352644.63825
SGTC_3070911700549.47 μM0.3225818779016Chembridge (Drug-like library)273.3021433.5313
SGTC_3098911903949.47 μM0.3030317161528Chembridge (Drug-like library)287.3287234.05313Golgi
SGTC_6690108-002126.2 μM0.317201ChemDiv (Drug-like library)292.331843.45724fatty acid desaturase (OLE1)
SGTC_1461k072-020353.8 μM0.2786895418714ChemDiv (Drug-like library)264.281982.60504
SGTC_2882905804458.44 μM0.27692318076804Chembridge (Drug-like library)273.3021433.62513
SGTC_577r071-000338.6 μM0.2727274568517ChemDiv (Drug-like library)276.332443.41523fatty acid desaturase (OLE1)
SGTC_480382-001855 μM0.271186762596ChemDiv (Drug-like library)231.204163.04214
SGTC_7294092-058594.1 μM0.2676062169061ChemDiv (Drug-like library)390.4500234.5504
SGTC_8600443-001625.1 μM0.2666675337942ChemDiv (Drug-like library)242.2450433.66513