cgp-37157

7-chloro-5-(2-chlorophenyl)-1,5-dihydro-4,1-benzothiazepin-2-one

A selective antagonist of mitochondrial Na+/Ca2+ exchange.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_465
Screen concentration 41.0 μM
Source ICCB bioactive library
PubChem CID 2688
SMILES C1C(=O)NC2=C(C=C(C=C2)Cl)C(S1)C3=CC=CC=C3Cl
Standardized SMILES Clc1ccc2NC(=O)CSC(c3ccccc3Cl)c2c1
Molecular weight 324.2249
ALogP 4.13
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.81
% growth inhibition (Hom. pool) 8.84


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2688
Download HIP data (tab-delimited text)  (excel)
Gene:ERG11(YHR007C)|FD-Score:-3.32|P-value:4.44E-4|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:FCP1(YMR277W)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.57||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:SPC24(YMR117C)|FD-Score:3.51|P-value:2.27E-4|Clearance:0.57||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:ERG11(YHR007C)|FD-Score:-3.32|P-value:4.44E-4|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:FCP1(YMR277W)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.57||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:SPC24(YMR117C)|FD-Score:3.51|P-value:2.27E-4|Clearance:0.57||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2688
Download HOP data (tab-delimited text)  (excel)
Gene:AHA1(YDR214W)|FD-Score:-3.09|P-value:9.93E-4||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:ALR2(YFL050C)|FD-Score:-3.23|P-value:6.12E-4||SGD DESC:Probable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; plays a role in regulating Ty1 transposition Gene:ARO1(YDR127W)|FD-Score:4.9|P-value:4.77E-7||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:6.93|P-value:2.07E-12||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:BCK1(YJL095W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BTS1(YPL069C)|FD-Score:-8.27|P-value:6.67E-17||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD32(YGR262C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:CGI121(YML036W)|FD-Score:-3.72|P-value:9.87E-5||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:GCN2(YDR283C)|FD-Score:9.89|P-value:2.20E-23||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN4(YEL009C)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HSP82(YPL240C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:IOC3(YFR013W)|FD-Score:3.76|P-value:8.57E-5||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRC21(YMR073C)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:NDI1(YML120C)|FD-Score:3.74|P-value:9.35E-5||SGD DESC:NADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; involved in Mn and H2O2 induced apoptosis; upon apoptotic stress, Ndip is activated in the mitochondria by N-terminal cleavage, and the truncated protein translocates to the cytoplasm to induce apoptosis; homolog of human AMID Gene:OPT2(YPR194C)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PTK2(YJR059W)|FD-Score:6.95|P-value:1.78E-12||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PYC2(YBR218C)|FD-Score:-3.21|P-value:6.60E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RIM9(YMR063W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:ROM2(YLR371W)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:SLG1(YOR008C)|FD-Score:4.45|P-value:4.23E-6||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:TRP1(YDR007W)|FD-Score:5.18|P-value:1.12E-7||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.86|P-value:3.49E-12||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:YBR238C(YBR238C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YCR085W(YCR085W_d)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR102C(YCR102C_p)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDR008C(YDR008C_d)|FD-Score:3.77|P-value:8.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:6.05|P-value:7.42E-10||SGD DESC:Putative protein of unknown function Gene:YNL120C(YNL120C_d)|FD-Score:4.6|P-value:2.10E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Gene:AHA1(YDR214W)|FD-Score:-3.09|P-value:9.93E-4||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:ALR2(YFL050C)|FD-Score:-3.23|P-value:6.12E-4||SGD DESC:Probable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; plays a role in regulating Ty1 transposition Gene:ARO1(YDR127W)|FD-Score:4.9|P-value:4.77E-7||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:6.93|P-value:2.07E-12||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:BCK1(YJL095W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BTS1(YPL069C)|FD-Score:-8.27|P-value:6.67E-17||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD32(YGR262C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:CGI121(YML036W)|FD-Score:-3.72|P-value:9.87E-5||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:GCN2(YDR283C)|FD-Score:9.89|P-value:2.20E-23||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN4(YEL009C)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HSP82(YPL240C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:IOC3(YFR013W)|FD-Score:3.76|P-value:8.57E-5||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRC21(YMR073C)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:NDI1(YML120C)|FD-Score:3.74|P-value:9.35E-5||SGD DESC:NADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; involved in Mn and H2O2 induced apoptosis; upon apoptotic stress, Ndip is activated in the mitochondria by N-terminal cleavage, and the truncated protein translocates to the cytoplasm to induce apoptosis; homolog of human AMID Gene:OPT2(YPR194C)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PTK2(YJR059W)|FD-Score:6.95|P-value:1.78E-12||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PYC2(YBR218C)|FD-Score:-3.21|P-value:6.60E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RIM9(YMR063W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:ROM2(YLR371W)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:SLG1(YOR008C)|FD-Score:4.45|P-value:4.23E-6||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:TRP1(YDR007W)|FD-Score:5.18|P-value:1.12E-7||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.86|P-value:3.49E-12||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:YBR238C(YBR238C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YCR085W(YCR085W_d)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR102C(YCR102C_p)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDR008C(YDR008C_d)|FD-Score:3.77|P-value:8.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:6.05|P-value:7.42E-10||SGD DESC:Putative protein of unknown function Gene:YNL120C(YNL120C_d)|FD-Score:4.6|P-value:2.10E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR277W3.591.67E-40.57FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
YMR117C3.512.27E-40.57SPC24Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
YHR083W2.940.001660.08SAM35Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane
YBR192W2.860.002150.13RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YGL001C2.730.003210.08ERG26C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis
YLR163C2.640.004100.02MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YJL031C2.630.004320.07BET4Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YMR208W2.560.005250.05ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YKL083W_d2.510.006000.00YKL083W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
YKL145W2.510.006050.03RPT1One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
YBR140C2.470.006670.05IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YGR114C_d2.430.007600.02YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YKL088W2.400.008130.07CAB3Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC
YPR136C_d2.330.009780.08YPR136C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9
YLR215C2.260.012100.02CDC123Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR283C9.892.20E-23GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YJR059W6.951.78E-12PTK2Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake
YDR035W6.932.07E-12ARO33-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
YDR354W6.863.49E-12TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YGL117W_p6.057.42E-10YGL117W_pPutative protein of unknown function
YDR007W5.181.12E-7TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YDR127W4.904.77E-7ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YNL120C_d4.602.10E-6YNL120C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene
YOR008C4.454.23E-6SLG1Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response
YFR009W4.102.03E-5GCN20Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
YDR008C_d3.778.05E-5YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YFR013W3.768.57E-5IOC3Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication
YML120C3.749.35E-5NDI1NADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; involved in Mn and H2O2 induced apoptosis; upon apoptotic stress, Ndip is activated in the mitochondria by N-terminal cleavage, and the truncated protein translocates to the cytoplasm to induce apoptosis; homolog of human AMID
YKL211C3.691.12E-4TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YBR238C3.651.30E-4YBR238CMitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span

GO enrichment analysis for SGTC_465
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4730SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0731707
0.4522.68E-295SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.075
0.4486.84E-289SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0857143
0.4413.36E-279SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.15873
0.4367.22E-273SGTC_6770929-0063 136.0 μMChemDiv (Drug-like library)15395830.142857
0.4312.05E-266SGTC_1649st011737 51.3 μMTimTec (Natural product derivative library)17610350.0526316
0.4283.05E-262SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0857143
0.4156.37E-245SGTC_2524avocatin b 10.6 μMMicrosource (Natural product library)6710748NA
0.4151.24E-244SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.0555556
0.4103.34E-238SGTC_2851486-1325 46.0 μMChemDiv (Drug-like library)223102300.112676
0.4052.98E-232SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.112676
0.4031.19E-229SGTC_20384100038 31.0 μMChembridge (Fragment library)35968030.135593plasma membrane duress
0.3912.86E-215SGTC_14274048-4900 550.0 nMChemDiv (Drug-like library)241325860.114754
0.3901.23E-213SGTC_24885268135 45.7 μMMiscellaneous12703560.166667
0.3884.88E-211SGTC_33089128332 17.6 μMChembridge (Drug-like library)173328050.157143plasma membrane duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_23609040895200 μM0.2909094914414Chembridge (Fragment library)239.6551.24613RPP1 & pyrimidine depletion
SGTC_14264048-448071.7 μM0.26666714224343ChemDiv (Drug-like library)290.767943.2920360S ribosome export
SGTC_1090efavirenz20.7 μM0.26153864139Miscellaneous315.674974.38115
SGTC_3239913375749.47 μM0.26153817036923Chembridge (Drug-like library)301.767443.42423
SGTC_2405ticlatone3.52 μM0.266258Miscellaneous185.630762.03912
SGTC_440929-005561.21 μM0.2592591379590ChemDiv (Drug-like library)239.678142.60613
SGTC_20775212015200 μM0.259259780900Chembridge (Fragment library)288.755362.12725
SGTC_232clozapine119 μM0.2539682818Miscellaneous326.823263.47614NEO1
SGTC_443clozapine122 μM0.2539682818Miscellaneous326.823263.47614NEO1-PIK1
SGTC_3323913856453.92 μM0.2543912313Chembridge (Drug-like library)263.290622.94312
SGTC_21015377231179.23 μM0.2413792844213Chembridge (Fragment library)256.708662.35514