geldanamycin

[(3R,5S,6R,7S,8E,10S,11S,12Z,14E)-6-hydroxy-5,11,21-trimethoxy-3,7,9,15-tetramethyl-16,20,22-trioxo-17-azabicyclo[16.3.1]docosa-1(21),8,12,14,18-pentaen-10-yl] carbamate

HSP90 inhibitor.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 

PubChem MeSH terms: Antibiotics, Antineoplastic;Antiviral Agents;Cysteine Proteinase Inhibitors;Enzyme Inhibitors



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_466
Screen concentration 89.2 μM
Source ICCB bioactive library
PubChem CID 5288382
SMILES CC1CC(C(C(C=C(C(C(C=CC=C(C(=O)NC2=CC(=O)C(=C(C1)C2=O)OC)C)OC)OC(=O)N)C)C)O)OC
Standardized SMILES COC1CC(C)CC2=C(OC)C(=O)C=C(NC(=O)C(=CC=CC(OC)C(OC(=O)N)C(=CC(C)C1O)C)C)C2=O
Molecular weight 560.6359
ALogP 2.36
H-bond donor count 3
H-bond acceptor count 9
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.69
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5288382
Download HIP data (tab-delimited text)  (excel)
Gene:NSA2(YER126C)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.11||SGD DESC:Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA Gene:RPB8(YOR224C)|FD-Score:-7.88|P-value:1.68E-15|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPO26(YPR187W)|FD-Score:-3.52|P-value:2.12E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPP1(YHR062C)|FD-Score:8.57|P-value:5.13E-18|Clearance:2.81||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS3(YNL178W)|FD-Score:3.57|P-value:1.78E-4|Clearance:0.76||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RPT1(YKL145W)|FD-Score:5.76|P-value:4.13E-9|Clearance:2.09||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RVB2(YPL235W)|FD-Score:-5.12|P-value:1.55E-7|Clearance:0||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:NSA2(YER126C)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.11||SGD DESC:Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA Gene:RPB8(YOR224C)|FD-Score:-7.88|P-value:1.68E-15|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPO26(YPR187W)|FD-Score:-3.52|P-value:2.12E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPP1(YHR062C)|FD-Score:8.57|P-value:5.13E-18|Clearance:2.81||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS3(YNL178W)|FD-Score:3.57|P-value:1.78E-4|Clearance:0.76||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RPT1(YKL145W)|FD-Score:5.76|P-value:4.13E-9|Clearance:2.09||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RVB2(YPL235W)|FD-Score:-5.12|P-value:1.55E-7|Clearance:0||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5288382
Download HOP data (tab-delimited text)  (excel)
Gene:APL5(YPL195W)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:COG8(YML071C)|FD-Score:3.86|P-value:5.73E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL7(YIR031C)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DFG5(YMR238W)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DRS2(YAL026C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:ECM2(YBR065C)|FD-Score:4.69|P-value:1.34E-6||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:ERG2(YMR202W)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:8.35|P-value:3.29E-17||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:HEM14(YER014W)|FD-Score:4.94|P-value:3.84E-7||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HHF1(YBR009C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HSP26(YBR072W)|FD-Score:4.38|P-value:5.86E-6||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:MMT2(YPL224C)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:NMD4(YLR363C)|FD-Score:-4.84|P-value:6.43E-7||SGD DESC:Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NTH2(YBR001C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PAU24(YBR301W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PHO88(YBR106W)|FD-Score:-3.17|P-value:7.71E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PIH1(YHR034C)|FD-Score:-3.81|P-value:6.84E-5||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PUS5(YLR165C)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:REV3(YPL167C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:SAC1(YKL212W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SHE4(YOR035C)|FD-Score:3.86|P-value:5.62E-5||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SPR1(YOR190W)|FD-Score:-3.3|P-value:4.86E-4||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:SWP82(YFL049W)|FD-Score:-3.2|P-value:6.98E-4||SGD DESC:Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions Gene:SYS1(YJL004C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TRP4(YDR354W)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBP7(YIL156W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions Gene:VPS36(YLR417W)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS41(YDR080W)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:YGL072C(YGL072C_d)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YMR158C-A(YMR158C-A_p)|FD-Score:4.05|P-value:2.56E-5||SGD DESC:Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene Gene:APL5(YPL195W)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:COG8(YML071C)|FD-Score:3.86|P-value:5.73E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL7(YIR031C)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DFG5(YMR238W)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DRS2(YAL026C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:ECM2(YBR065C)|FD-Score:4.69|P-value:1.34E-6||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:ERG2(YMR202W)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:8.35|P-value:3.29E-17||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:HEM14(YER014W)|FD-Score:4.94|P-value:3.84E-7||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HHF1(YBR009C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HSP26(YBR072W)|FD-Score:4.38|P-value:5.86E-6||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:MMT2(YPL224C)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:NMD4(YLR363C)|FD-Score:-4.84|P-value:6.43E-7||SGD DESC:Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NTH2(YBR001C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PAU24(YBR301W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PHO88(YBR106W)|FD-Score:-3.17|P-value:7.71E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PIH1(YHR034C)|FD-Score:-3.81|P-value:6.84E-5||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PUS5(YLR165C)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:REV3(YPL167C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:SAC1(YKL212W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SHE4(YOR035C)|FD-Score:3.86|P-value:5.62E-5||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SPR1(YOR190W)|FD-Score:-3.3|P-value:4.86E-4||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:SWP82(YFL049W)|FD-Score:-3.2|P-value:6.98E-4||SGD DESC:Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions Gene:SYS1(YJL004C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TRP4(YDR354W)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBP7(YIL156W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions Gene:VPS36(YLR417W)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS41(YDR080W)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:YGL072C(YGL072C_d)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YMR158C-A(YMR158C-A_p)|FD-Score:4.05|P-value:2.56E-5||SGD DESC:Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C8.575.13E-182.81RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YKL145W5.764.13E-92.09RPT1One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
YER126C3.681.18E-40.11NSA2Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA
YNL178W3.571.78E-40.76RPS3Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3
YOL127W2.810.002440.14RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
YJR007W2.680.003700.03SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
YOR340C2.650.004050.04RPA43RNA polymerase I subunit A43
YBL050W2.610.004520.12SEC17Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP
YBR087W2.490.006360.06RFC5Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YJL014W2.430.007470.08CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YOR341W2.350.009370.04RPA190RNA polymerase I largest subunit A190
YER038C2.310.010300.04KRE29Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
YLR106C2.280.011400.01MDN1Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus
YOL139C2.270.011600.06CDC33mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth
YJL081C2.210.013400.07ARP4Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL012W8.353.29E-17ERG4C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
YER014W4.943.84E-7HEM14Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides
YBR065C4.691.34E-6ECM2Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p
YBR072W4.385.86E-6HSP26Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity
YBR301W4.151.63E-5PAU24Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth
YDR080W4.072.36E-5VPS41Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport
YMR158C-A_p4.052.56E-5YMR158C-A_pPutative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene
YOR035C3.865.62E-5SHE4Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization
YML071C3.865.73E-5COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YKL212W3.641.38E-4SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YPL167C3.512.21E-4REV3Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair
YJL004C3.512.21E-4SYS1Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
YBR009C3.462.71E-4HHF1Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity
YGL072C_d3.413.26E-4YGL072C_dDubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
YBR001C3.413.27E-4NTH2Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_466
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1831.50E-45SGTC_2526patulin 37.6 μMMicrosource (Natural product library)46960.101124
0.1701.59E-39SGTC_22006600604 21.9 μMChembridge (Fragment library)29047770.0869565tubulin folding & SWR complex
0.1661.23E-37SGTC_13691866-0067 208.0 μMChemDiv (Drug-like library)35313550.036036RPP1 & pyrimidine depletion
0.1645.07E-37SGTC_9751335-0046 28.5 μMChemDiv (Drug-like library)164867400.0740741RPP1 & pyrimidine depletion
0.1611.40E-35SGTC_20775212015 200.0 μMChembridge (Fragment library)7809000.0412371
0.1581.73E-34SGTC_8410251-0215 91.2 μMChemDiv (Drug-like library)38886140.0344828
0.1561.62E-33SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.0841122RPP1 & pyrimidine depletion
0.1497.28E-31SGTC_481bay k-8644 50.0 μMICCB bioactive library23030.114286
0.1423.82E-28SGTC_6790422-0091 41.4 μMChemDiv (Drug-like library)6273840.0319149Golgi
0.1424.04E-28SGTC_1581himbacine 57.9 μMTimTec (Pure natural product library)52818250.0733945Golgi
0.1402.26E-27SGTC_31669102297 49.5 μMChembridge (Drug-like library)252370720.0571429Golgi
0.1395.53E-27SGTC_391gliotoxin 8.3 μMMiscellaneous62230.122642
0.1396.81E-27SGTC_540159-0055 9.0 μMChemDiv (Drug-like library)33190990.0181818ERG2
0.1398.97E-27SGTC_265mycophenolic acid 32.9 μMMiscellaneous4465410.11215RPP1 & pyrimidine depletion
0.1381.60E-26SGTC_1464k208-0027 110.0 μMChemDiv (Drug-like library)X14640.0672269

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2745rifampicin24.3 μM0.23880625141428Miscellaneous822.940221.388615
SGTC_15034585-001261.3 μM0.1960782922052ChemDiv (Drug-like library)338.464943.81214
SGTC_2645abamectin100 μM0.1888115702208Microsource (Natural product library)873.076884.692314heme requiring
SGTC_2713st07914953.4 μM0.1826923103253TimTec (Natural product derivative library)374.430842.91635
SGTC_2665lovastatin100 μM0.1801853232Miscellaneous404.539644.21815
SGTC_504lovastatin124 μM0.1801853232ICCB bioactive library404.539644.21815
SGTC_1545lovastatin49.4 μM0.1801812923612TimTec (Pure natural product library)404.539644.21815
SGTC_1104tacrolimus29.8 μM0.16546823581796NIH Clinical Collection804.018164.832312
SGTC_2689tacrolimus100 μM0.16546823581796NIH Clinical Collection804.018164.832312
SGTC_1844rapamycin4 nM0.1610745284616Miscellaneous914.171866.295313
SGTC_1873rapamycin2 nM0.1610745284616Miscellaneous914.171866.295313
SGTC_233rapamycin362 pM0.1610745284616Miscellaneous914.171866.295313
SGTC_2679rapamycin1 nM0.1610745284616Microsource (Natural product library)914.171866.295313
SGTC_257daunorubicin18.5 μM0.15702530323Miscellaneous527.519860.628511anthracycline transcription coupled DNA repair
SGTC_2662daunorubicin22.37 μM0.15702530323Miscellaneous527.519860.628511anthracycline transcription coupled DNA repair
SGTC_2746daunorubicin9.88 μM0.15702530323Miscellaneous527.519860.628511anthracycline transcription coupled DNA repair
SGTC_2657nystatin74.44 μM0.1562511468707Miscellaneous926.0949-2.6041218
SGTC_277nystatin15.5 μM0.1562511468707Miscellaneous926.0949-2.6041218
SGTC_272doxorubicin8.13 μM0.15322631703Miscellaneous543.51926-0.044612anthracycline transcription coupled DNA repair