gf-109203x

3-[1-[3-(dimethylamino)propyl]indol-3-yl]-4-(1H-indol-3-yl)pyrrole-2,5-dione

A protein kinase C inhibitor.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 

PubChem MeSH terms: Anti-Inflammatory Agents;Enzyme Inhibitors



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_468
Screen concentration 121.0 μM
Source ICCB bioactive library
PubChem CID 2396
SMILES CN(C)CCCN1C=C(C2=CC=CC=C21)C3=C(C(=O)NC3=O)C4=CNC5=CC=CC=C54
Standardized SMILES CN(C)CCCn1cc(C2=C(C(=O)NC2=O)c3c[nH]c4ccccc34)c5ccccc15
Molecular weight 412.4837
ALogP 3.56
H-bond donor count 2
H-bond acceptor count 3
Response signature amide catabolism

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.12
% growth inhibition (Hom. pool) 4.16


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2396
Download HIP data (tab-delimited text)  (excel)
Gene:DRE2(YKR071C)|FD-Score:-3.68|P-value:1.18E-4|Clearance:0||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:ERG12(YMR208W)|FD-Score:4.84|P-value:6.64E-7|Clearance:1.33||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:IMP3(YHR148W)|FD-Score:-3.4|P-value:3.33E-4|Clearance:0||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:MAK21(YDR060W)|FD-Score:3.46|P-value:2.66E-4|Clearance:0.11||SGD DESC:Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein Gene:PMI40(YER003C)|FD-Score:3.36|P-value:3.96E-4|Clearance:0.5||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PTI1(YGR156W)|FD-Score:4.79|P-value:8.35E-7|Clearance:1.33||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RRS1(YOR294W)|FD-Score:4.8|P-value:7.96E-7|Clearance:1.33||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:TRS20(YBR254C)|FD-Score:-3.12|P-value:9.12E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder Gene:DRE2(YKR071C)|FD-Score:-3.68|P-value:1.18E-4|Clearance:0||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:ERG12(YMR208W)|FD-Score:4.84|P-value:6.64E-7|Clearance:1.33||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:IMP3(YHR148W)|FD-Score:-3.4|P-value:3.33E-4|Clearance:0||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:MAK21(YDR060W)|FD-Score:3.46|P-value:2.66E-4|Clearance:0.11||SGD DESC:Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein Gene:PMI40(YER003C)|FD-Score:3.36|P-value:3.96E-4|Clearance:0.5||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PTI1(YGR156W)|FD-Score:4.79|P-value:8.35E-7|Clearance:1.33||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RRS1(YOR294W)|FD-Score:4.8|P-value:7.96E-7|Clearance:1.33||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:TRS20(YBR254C)|FD-Score:-3.12|P-value:9.12E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2396
Download HOP data (tab-delimited text)  (excel)
Gene:ARP1(YHR129C)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:ASI1(YMR119W)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ATG3(YNR007C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:BEM3(YPL115C)|FD-Score:-3.11|P-value:9.50E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:BNI1(YNL271C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BTS1(YPL069C)|FD-Score:-4.83|P-value:6.98E-7||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CMC4(YMR194C-B)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs Gene:CTK1(YKL139W)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DAL81(YIR023W)|FD-Score:4.28|P-value:9.14E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:FMP46(YKR049C)|FD-Score:-3.83|P-value:6.46E-5||SGD DESC:Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GAL1(YBR020W)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication Gene:IOC4(YMR044W)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:LEM3(YNL323W)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MRPL15(YLR312W-A)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:PIB2(YGL023C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PRO2(YOR323C)|FD-Score:7.96|P-value:8.73E-16||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RGP1(YDR137W)|FD-Score:-3.32|P-value:4.50E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPS1A(YLR441C)|FD-Score:-3.26|P-value:5.47E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:SPI1(YER150W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SUL2(YLR092W)|FD-Score:3.88|P-value:5.26E-5||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:TOM70(YNL121C)|FD-Score:-3.12|P-value:9.07E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:YLR358C(YLR358C_p)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YPR114W(YPR114W_p)|FD-Score:4.46|P-value:4.19E-6||SGD DESC:Putative protein of unknown function Gene:ARP1(YHR129C)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:ASI1(YMR119W)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ATG3(YNR007C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:BEM3(YPL115C)|FD-Score:-3.11|P-value:9.50E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:BNI1(YNL271C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BTS1(YPL069C)|FD-Score:-4.83|P-value:6.98E-7||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CMC4(YMR194C-B)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs Gene:CTK1(YKL139W)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DAL81(YIR023W)|FD-Score:4.28|P-value:9.14E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:FMP46(YKR049C)|FD-Score:-3.83|P-value:6.46E-5||SGD DESC:Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GAL1(YBR020W)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication Gene:IOC4(YMR044W)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:LEM3(YNL323W)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MRPL15(YLR312W-A)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:PIB2(YGL023C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PRO2(YOR323C)|FD-Score:7.96|P-value:8.73E-16||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RGP1(YDR137W)|FD-Score:-3.32|P-value:4.50E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPS1A(YLR441C)|FD-Score:-3.26|P-value:5.47E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:SPI1(YER150W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SUL2(YLR092W)|FD-Score:3.88|P-value:5.26E-5||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:TOM70(YNL121C)|FD-Score:-3.12|P-value:9.07E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:YLR358C(YLR358C_p)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YPR114W(YPR114W_p)|FD-Score:4.46|P-value:4.19E-6||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR208W4.846.64E-71.33ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YOR294W4.807.96E-71.33RRS1Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
YGR156W4.798.35E-71.33PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
YDR060W3.462.66E-40.11MAK21Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
YER003C3.363.96E-40.50PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YNR038W2.860.002130.01DBP6Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family
YNL317W2.840.002220.27PFS2Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex
YBR136W2.580.004970.08MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
YOR278W2.500.006270.06HEM4Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
YBR243C2.440.007320.01ALG7UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
YPL175W2.430.007570.08SPT14UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins
YHR172W2.350.009330.01SPC97Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YPR187W2.340.009550.00RPO26RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit
YMR309C2.340.009680.03NIP1eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection
YGR065C2.310.010500.12VHT1High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR323C7.968.73E-16PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YPR114W_p4.464.19E-6YPR114W_pPutative protein of unknown function
YIR023W4.289.14E-6DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YNR007C4.241.13E-5ATG3E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p
YMR119W4.121.88E-5ASI1Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication
YLR092W3.885.26E-5SUL2High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates
YGL023C3.621.47E-4PIB2Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27
YKL139W3.502.36E-4CTK1Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
YLR358C_p3.492.38E-4YLR358C_pProtein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W
YNL271C3.363.88E-4BNI1Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1
YNL323W3.344.15E-4LEM3Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane
YER150W3.265.63E-4SPI1GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p
YMR044W3.245.89E-4IOC4Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing
YHR129C3.206.79E-4ARP1Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin
YBR020W3.187.45E-4GAL1Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication

GO enrichment analysis for SGTC_468
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2441.52E-80SGTC_4711-acyl-paf 4.0 μMICCB bioactive library167593670.0434783DNA intercalators
0.2231.94E-67SGTC_7003453-2283 142.0 μMChemDiv (Drug-like library)5656640.0769231amide catabolism
0.2042.84E-56SGTC_469w7 172.0 μMICCB bioactive library451155700.108108ERG2
0.1995.57E-54SGTC_6330437-0093 97.7 μMChemDiv (Drug-like library)28311180.0808081DNA intercalators
0.1866.62E-47SGTC_8580438-0351 2.8 μMChemDiv (Drug-like library)27991190.0487805copper-dependent oxidative stress
0.1811.72E-44SGTC_8670518-0118 155.0 μMChemDiv (Drug-like library)68481580.0779221DNA intercalators
0.1784.35E-43SGTC_512prima-1 270.0 μMICCB bioactive library3229680.057971DNA intercalators
0.1743.44E-41SGTC_1973st070275 50.2 μMTimTec (Natural product derivative library)171183340.173333
0.1743.69E-41SGTC_1459k035-0031 35.7 μMChemDiv (Drug-like library)38771280.119403DNA intercalators
0.1503.98E-31SGTC_486niguldipine 82.0 μMMiscellaneous12360.105769amide catabolism
0.1463.03E-29SGTC_30409091894 49.5 μMChembridge (Drug-like library)252369660.0952381DNA intercalators
0.1381.52E-26SGTC_2501sodium tetradecyl sulfate 13.4 μMMicrosource (Natural product library)236657720.0512821
0.1367.39E-26SGTC_412gemfibrozil 100.0 μMMiscellaneous34630.0897436DNA intercalators
0.1368.29E-26SGTC_28459003024 43.0 μMChembridge (Drug-like library)29901730.0864198amide catabolism
0.1292.79E-23SGTC_467ro 31-8220 72.8 μMICCB bioactive library50830.477612calcium & mitochondrial duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_467ro 31-822072.8 μM0.4776125083ICCB bioactive library457.547423.86634calcium & mitochondrial duress
SGTC_2385hr heat shock (37°C) + nsc-6384328.93 μM0.30137368090Miscellaneous406.904625.23213heat shock/prefoldin
SGTC_419nsc-638432893 μM0.30137368090Miscellaneous406.904625.23213DNA damage response
SGTC_8910929-0073133 μM0.2923085603632ChemDiv (Drug-like library)244.269122.23523
SGTC_12630774-120864.8 μM0.292308906585ChemDiv (Drug-like library)304.342543.51612
SGTC_2591skatole100 μM0.2777786736Microsource (Natural product library)131.174462.60910
SGTC_452diindolylmethane8 μM0.2727273071ICCB bioactive library246.306464.39120RNA processing & uracil transport
SGTC_1774st04837148.47 μM0.2647064242727TimTec (Natural product derivative library)318.415483.47412RNA processing & uracil transport
SGTC_2588indole-3-carbinol100 μM0.2631583712Microsource (Natural product library)147.173861.51821
SGTC_2017513292760.65 μM0.2539688367Chembridge (Fragment library)188.268842.45121