w7

N'-[(5-chloronaphthalen-1-yl)methyl]hexane-1,6-diamine

A P450 inhibitor.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_469
Screen concentration 172.0 μM
Source ICCB bioactive library
PubChem CID 45115570
SMILES C1=CC2=C(C=CC=C2Cl)C(=C1)CNCCCCCCN
Standardized SMILES NCCCCCCNCc1cccc2c(Cl)cccc12
Molecular weight 290.8309
ALogP 3.67
H-bond donor count 2
H-bond acceptor count 2
Response signature ERG2

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.08
% growth inhibition (Hom. pool) 6.37


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 45115570
Download HIP data (tab-delimited text)  (excel)
Gene:ERG12(YMR208W)|FD-Score:5.99|P-value:1.05E-9|Clearance:1.12||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:MAK21(YDR060W)|FD-Score:3.93|P-value:4.25E-5|Clearance:0.6||SGD DESC:Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein Gene:MCM7(YBR202W)|FD-Score:3.13|P-value:8.85E-4|Clearance:0.21||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:PMI40(YER003C)|FD-Score:3.28|P-value:5.24E-4|Clearance:0.08||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PTI1(YGR156W)|FD-Score:3.98|P-value:3.44E-5|Clearance:0.05||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RHO1(YPR165W)|FD-Score:3.2|P-value:6.84E-4|Clearance:0.07||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RRS1(YOR294W)|FD-Score:5.1|P-value:1.74E-7|Clearance:1.12||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:TSR4(YOL022C)|FD-Score:-4.07|P-value:2.36E-5|Clearance:0||SGD DESC:Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 Gene:YDL196W(YDL196W_d)|FD-Score:3.33|P-value:4.31E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:ERG12(YMR208W)|FD-Score:5.99|P-value:1.05E-9|Clearance:1.12||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:MAK21(YDR060W)|FD-Score:3.93|P-value:4.25E-5|Clearance:0.6||SGD DESC:Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein Gene:MCM7(YBR202W)|FD-Score:3.13|P-value:8.85E-4|Clearance:0.21||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:PMI40(YER003C)|FD-Score:3.28|P-value:5.24E-4|Clearance:0.08||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PTI1(YGR156W)|FD-Score:3.98|P-value:3.44E-5|Clearance:0.05||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RHO1(YPR165W)|FD-Score:3.2|P-value:6.84E-4|Clearance:0.07||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RRS1(YOR294W)|FD-Score:5.1|P-value:1.74E-7|Clearance:1.12||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:TSR4(YOL022C)|FD-Score:-4.07|P-value:2.36E-5|Clearance:0||SGD DESC:Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 Gene:YDL196W(YDL196W_d)|FD-Score:3.33|P-value:4.31E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 45115570
Download HOP data (tab-delimited text)  (excel)
Gene:APM1(YPL259C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:ARC18(YLR370C)|FD-Score:4.66|P-value:1.59E-6||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ASI1(YMR119W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ATC1(YDR184C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:CIT1(YNR001C)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:COA1(YIL157C)|FD-Score:3.83|P-value:6.52E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:COG8(YML071C)|FD-Score:-4.79|P-value:8.39E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTK1(YKL139W)|FD-Score:3.83|P-value:6.39E-5||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:EMP70(YLR083C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:END3(YNL084C)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERG2(YMR202W)|FD-Score:7.43|P-value:5.34E-14||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:HCS1(YKL017C)|FD-Score:-3.16|P-value:7.97E-4||SGD DESC:Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities Gene:HEH2(YDR458C)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:IDP2(YLR174W)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication Gene:IMP2'(YIL154C)|FD-Score:7.88|P-value:1.58E-15||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:MNN2(YBR015C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:ORM1(YGR038W)|FD-Score:4.66|P-value:1.55E-6||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PHO4(YFR034C)|FD-Score:6.82|P-value:4.41E-12||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PIN4(YBL051C)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:RCR2(YDR003W)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Vacuolar protein; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SLT2(YHR030C)|FD-Score:4.58|P-value:2.36E-6||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:TIR4(YOR009W)|FD-Score:-3.15|P-value:8.07E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock Gene:UFD2(YDL190C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:VTA1(YLR181C)|FD-Score:3.83|P-value:6.53E-5||SGD DESC:Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain Gene:YAL056C-A(YAL056C-A_d)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL055C(YBL055C)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YCL042W(YCL042W_p)|FD-Score:4.58|P-value:2.37E-6||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YCR087C-A(YCR087C-A_p)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YFL012W(YFL012W_p)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YGL176C(YGL176C_p)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YKL151C(YKL151C)|FD-Score:-3.81|P-value:6.83E-5||SGD DESC:NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YLR252W(YLR252W_d)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YMR001C-A(YMR001C-A_p)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Putative protein of unknown function Gene:YMR173W-A(YMR173W-A_d)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YPS7(YDR349C)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum Gene:APM1(YPL259C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:ARC18(YLR370C)|FD-Score:4.66|P-value:1.59E-6||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ASI1(YMR119W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ATC1(YDR184C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:CIT1(YNR001C)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:COA1(YIL157C)|FD-Score:3.83|P-value:6.52E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:COG8(YML071C)|FD-Score:-4.79|P-value:8.39E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTK1(YKL139W)|FD-Score:3.83|P-value:6.39E-5||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:EMP70(YLR083C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:END3(YNL084C)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERG2(YMR202W)|FD-Score:7.43|P-value:5.34E-14||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:HCS1(YKL017C)|FD-Score:-3.16|P-value:7.97E-4||SGD DESC:Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities Gene:HEH2(YDR458C)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:IDP2(YLR174W)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication Gene:IMP2'(YIL154C)|FD-Score:7.88|P-value:1.58E-15||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:MNN2(YBR015C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:ORM1(YGR038W)|FD-Score:4.66|P-value:1.55E-6||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PHO4(YFR034C)|FD-Score:6.82|P-value:4.41E-12||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PIN4(YBL051C)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:RCR2(YDR003W)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Vacuolar protein; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SLT2(YHR030C)|FD-Score:4.58|P-value:2.36E-6||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:TIR4(YOR009W)|FD-Score:-3.15|P-value:8.07E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock Gene:UFD2(YDL190C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:VTA1(YLR181C)|FD-Score:3.83|P-value:6.53E-5||SGD DESC:Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain Gene:YAL056C-A(YAL056C-A_d)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL055C(YBL055C)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YCL042W(YCL042W_p)|FD-Score:4.58|P-value:2.37E-6||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YCR087C-A(YCR087C-A_p)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YFL012W(YFL012W_p)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YGL176C(YGL176C_p)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YKL151C(YKL151C)|FD-Score:-3.81|P-value:6.83E-5||SGD DESC:NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YLR252W(YLR252W_d)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YMR001C-A(YMR001C-A_p)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Putative protein of unknown function Gene:YMR173W-A(YMR173W-A_d)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YPS7(YDR349C)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR208W5.991.05E-91.12ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YOR294W5.101.74E-71.12RRS1Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
YGR156W3.983.44E-50.05PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
YDR060W3.934.25E-50.60MAK21Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
YDL196W_d3.334.31E-40.05YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YER003C3.285.24E-40.08PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YPR165W3.206.84E-40.07RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YBR202W3.138.85E-40.21MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YOR278W2.910.001780.04HEM4Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
YNL317W2.880.002010.10PFS2Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex
YHR172W2.780.002760.05SPC97Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YDL058W2.730.003190.02USO1Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
YKL180W2.710.003370.01RPL17ARibosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YER147C2.700.003500.09SCC4Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX
YPR094W2.610.004510.00RDS3Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIL154C7.881.58E-15IMP2'Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YMR202W7.435.34E-14ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YFR034C6.824.41E-12PHO4Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability
YNR001C4.741.05E-6CIT1Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication
YGR038W4.661.55E-6ORM1Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control
YLR370C4.661.59E-6ARC18Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YHR030C4.582.36E-6SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YCL042W_p4.582.37E-6YCL042W_pPutative protein of unknown function; epitope-tagged protein localizes to the cytoplasm
YNL084C4.121.90E-5END3EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
YDR458C4.062.48E-5HEH2Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle
YKL139W3.836.39E-5CTK1Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
YIL157C3.836.52E-5COA1Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly
YLR181C3.836.53E-5VTA1Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain
YAL056C-A_d3.681.16E-4YAL056C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YPL259C3.631.41E-4APM1Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting

GO enrichment analysis for SGTC_469
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2667.23E-96SGTC_2523spermine 47.7 μMMicrosource (Natural product library)11030.314286calcium & mitochondrial duress
0.2641.99E-94SGTC_500h-89 112.0 μMICCB bioactive library4492410.136986cell wall signaling
0.2495.21E-84SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.0655738ERG2
0.2251.71E-68SGTC_400oxethazaine 37.5 μMMiscellaneous46210.078125calcium & mitochondrial duress
0.2131.23E-61SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.149254ERG2
0.2061.13E-57SGTC_540159-0055 9.0 μMChemDiv (Drug-like library)33190990.104478ERG2
0.2042.84E-56SGTC_468gf-109203x 121.0 μMICCB bioactive library23960.108108amide catabolism
0.2003.13E-54SGTC_14504112-4260 37.6 μMChemDiv (Drug-like library)16331750.128571calcium & mitochondrial duress
0.1991.54E-53SGTC_23007976697 71.6 μMChembridge (Fragment library)22361090.254902ERG2
0.1982.09E-53SGTC_9943910-0338 138.0 μMChemDiv (Drug-like library)34794810.0921053calcium & mitochondrial duress
0.1943.34E-51SGTC_4711-acyl-paf 4.0 μMICCB bioactive library167593670.038961DNA intercalators
0.1927.31E-50SGTC_1874staurosporine 7.6 μMICCB bioactive library468641930.0795455cell wall signaling
0.1903.46E-49SGTC_960929-0093 37.3 μMChemDiv (Drug-like library)69915790.121212calcium & mitochondrial duress
0.1891.67E-48SGTC_13772944-0135 198.0 μMChemDiv (Drug-like library)18941370.0945946calcium & mitochondrial duress
0.1811.35E-44SGTC_5820987-0078 156.0 μMChemDiv (Drug-like library)16257650.134328calcium & mitochondrial duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2523spermine47.74 μM0.3142861103Microsource (Natural product library)202.34024-1.50444calcium & mitochondrial duress
SGTC_23749071832200 μM0.27659617381698Chembridge (Fragment library)222.111622.51722endomembrane recycling
SGTC_2300797669771.57 μM0.2549022236109Chembridge (Fragment library)238.756362.28912ERG2
SGTC_2979901255245.46 μM0.25454517100905Chembridge (Drug-like library)282.724343.03612
SGTC_22577960413200 μM0.252974689Chembridge (Fragment library)237.725242.53122NEO1-PIK1
SGTC_3255913907249.47 μM0.2517646060Chembridge (Drug-like library)309.811043.48213
SGTC_234nsc-1738310.7 μM0.238095408974Miscellaneous344.901444.69112ERG2
SGTC_23549060492162 μM0.2264156471895Chembridge (Fragment library)201.26761.27823
SGTC_3211912878149.47 μM0.22033917194361Chembridge (Drug-like library)294.756643.55423
SGTC_2944906734625.62 μM0.2142866473075Chembridge (Drug-like library)324.20184.1723