0149-0135

4-[(3,5-ditert-butyl-4-hydroxyphenyl)methyl]benzene-1,2-diol

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_47
Screen concentration 24.2 μM
Source ChemDiv (Drug-like library)
PubChem CID 5239155
SMILES CC(C)(C)C1=CC(=CC(=C1O)C(C)(C)C)CC2=CC(=C(C=C2)O)O
Standardized SMILES CC(C)(C)c1cc(Cc2ccc(O)c(O)c2)cc(c1O)C(C)(C)C
Molecular weight 328.4452
ALogP 5.88
H-bond donor count 3
H-bond acceptor count 3
Response signature TSC3-RPN4

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.64
% growth inhibition (Hom. pool) 2.58


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5239155
Download HIP data (tab-delimited text)  (excel)
Gene:ABD1(YBR236C)|FD-Score:7.13|P-value:5.10E-13|Clearance:1.71||SGD DESC:Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA Gene:CLP1(YOR250C)|FD-Score:5.42|P-value:2.96E-8|Clearance:0.63||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:EPL1(YFL024C)|FD-Score:-3.29|P-value:5.01E-4|Clearance:0||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:LSM3(YLR438C-A)|FD-Score:4.12|P-value:1.87E-5|Clearance:0.17||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MEX67(YPL169C)|FD-Score:3.43|P-value:3.02E-4|Clearance:0.06||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MSL5(YLR116W)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.07||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:NIC96(YFR002W)|FD-Score:3.47|P-value:2.58E-4|Clearance:0.04||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:ORC1(YML065W)|FD-Score:-4.15|P-value:1.69E-5|Clearance:0||SGD DESC:Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity Gene:PDC2(YDR081C)|FD-Score:3.3|P-value:4.83E-4|Clearance:0.11||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:RPA43(YOR340C)|FD-Score:4.79|P-value:8.19E-7|Clearance:0.67||SGD DESC:RNA polymerase I subunit A43 Gene:SMD2(YLR275W)|FD-Score:3.37|P-value:3.75E-4|Clearance:0.07||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SMP3(YOR149C)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.14||SGD DESC:Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure Gene:SUA5(YGL169W)|FD-Score:3.96|P-value:3.80E-5|Clearance:0.42||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:YCG1(YDR325W)|FD-Score:-3.09|P-value:9.96E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:ABD1(YBR236C)|FD-Score:7.13|P-value:5.10E-13|Clearance:1.71||SGD DESC:Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA Gene:CLP1(YOR250C)|FD-Score:5.42|P-value:2.96E-8|Clearance:0.63||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:EPL1(YFL024C)|FD-Score:-3.29|P-value:5.01E-4|Clearance:0||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:LSM3(YLR438C-A)|FD-Score:4.12|P-value:1.87E-5|Clearance:0.17||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MEX67(YPL169C)|FD-Score:3.43|P-value:3.02E-4|Clearance:0.06||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MSL5(YLR116W)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.07||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:NIC96(YFR002W)|FD-Score:3.47|P-value:2.58E-4|Clearance:0.04||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:ORC1(YML065W)|FD-Score:-4.15|P-value:1.69E-5|Clearance:0||SGD DESC:Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity Gene:PDC2(YDR081C)|FD-Score:3.3|P-value:4.83E-4|Clearance:0.11||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:RPA43(YOR340C)|FD-Score:4.79|P-value:8.19E-7|Clearance:0.67||SGD DESC:RNA polymerase I subunit A43 Gene:SMD2(YLR275W)|FD-Score:3.37|P-value:3.75E-4|Clearance:0.07||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SMP3(YOR149C)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.14||SGD DESC:Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure Gene:SUA5(YGL169W)|FD-Score:3.96|P-value:3.80E-5|Clearance:0.42||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:YCG1(YDR325W)|FD-Score:-3.09|P-value:9.96E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5239155
Download HOP data (tab-delimited text)  (excel)
Gene:AAC1(YMR056C)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:ADO1(YJR105W)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:APQ13(YJL075C_d)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate Gene:ASE1(YOR058C)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:COG6(YNL041C)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ9(YLR201C)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes Gene:CPD1(YGR247W)|FD-Score:-3.18|P-value:7.49E-4||SGD DESC:Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress Gene:CTF4(YPR135W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:CYT2(YKL087C)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:DIA3(YDL024C)|FD-Score:4.87|P-value:5.49E-7||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:DNF3(YMR162C)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase Gene:EFG1(YGR271C-A)|FD-Score:4.48|P-value:3.66E-6||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ERP5(YHR110W)|FD-Score:-3.23|P-value:6.20E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FRK1(YPL141C)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:GLO2(YDR272W)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:ISR1(YPR106W)|FD-Score:-3.31|P-value:4.75E-4||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:KAR9(YPL269W)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:KEL2(YGR238C)|FD-Score:-4.96|P-value:3.57E-7||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:KRE2(YDR483W)|FD-Score:3.98|P-value:3.48E-5||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LEA1(YPL213W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:LIP2(YLR239C)|FD-Score:5.02|P-value:2.57E-7||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:LOC1(YFR001W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:MDY2(YOL111C)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MET17(YLR303W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MPC2(YHR162W)|FD-Score:5.4|P-value:3.34E-8||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium Gene:MSA2(YKR077W)|FD-Score:4.39|P-value:5.66E-6||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MTD1(YKR080W)|FD-Score:6.49|P-value:4.27E-11||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:NIT2(YJL126W)|FD-Score:3.77|P-value:8.17E-5||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:OLA1(YBR025C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress Gene:OSH2(YDL019C)|FD-Score:4.38|P-value:5.89E-6||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAF1(YBR279W)|FD-Score:4.75|P-value:9.95E-7||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PAN3(YKL025C)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PEP12(YOR036W)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEX32(YBR168W)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PHR1(YOR386W)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:PRM4(YPL156C)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift Gene:PTC1(YDL006W)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PYC1(YGL062W)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:RAD14(YMR201C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RAD34(YDR314C)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Protein involved in nucleotide excision repair (NER); homologous to RAD4 Gene:RPL16A(YIL133C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RRT2(YBR246W)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:RTS3(YGR161C_p)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SCO2(YBR024W)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication Gene:SFB3(YHR098C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SIP5(YMR140W)|FD-Score:-7.84|P-value:2.18E-15||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SLH1(YGR271W)|FD-Score:4.93|P-value:4.03E-7||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SSO2(YMR183C)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p Gene:TMA23(YMR269W)|FD-Score:4.5|P-value:3.35E-6||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TRM11(YOL124C)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain Gene:TSC3(YBR058C-A)|FD-Score:15.1|P-value:7.74E-52||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TSR2(YLR435W)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:YAL037W(YAL037W_p)|FD-Score:-3.14|P-value:8.33E-4||SGD DESC:Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication Gene:YBL096C(YBL096C_d)|FD-Score:-4.19|P-value:1.40E-5||SGD DESC:Non-essential protein of unknown function Gene:YBR027C(YBR027C_d)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR015C(YCR015C_p)|FD-Score:-3.3|P-value:4.86E-4||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YDL211C(YDL211C_p)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR222W(YDR222W_p)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YDR249C(YDR249C_p)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:Putative protein of unknown function Gene:YER184C(YER184C_p)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source Gene:YHR007C-A(YHR007C-A_p)|FD-Score:-3.19|P-value:7.24E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YHR095W(YHR095W_d)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL055W(YJL055W_p)|FD-Score:5.71|P-value:5.57E-9||SGD DESC:Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU Gene:YJL206C(YJL206C_p)|FD-Score:3.32|P-value:4.55E-4||SGD DESC:Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment Gene:YKL069W(YKL069W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YMR122C(YMR122C_d)|FD-Score:5.64|P-value:8.70E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL143C(YNL143C_p)|FD-Score:4.89|P-value:5.17E-7||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOL097W-A(YOL097W-A_p)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR376W-A(YOR376W-A_p)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPL272C(YPL272C_p)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Putative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene Gene:YPR099C(YPR099C_d)|FD-Score:-3.44|P-value:2.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W Gene:AAC1(YMR056C)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:ADO1(YJR105W)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:APQ13(YJL075C_d)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate Gene:ASE1(YOR058C)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:COG6(YNL041C)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ9(YLR201C)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes Gene:CPD1(YGR247W)|FD-Score:-3.18|P-value:7.49E-4||SGD DESC:Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress Gene:CTF4(YPR135W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:CYT2(YKL087C)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:DIA3(YDL024C)|FD-Score:4.87|P-value:5.49E-7||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:DNF3(YMR162C)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase Gene:EFG1(YGR271C-A)|FD-Score:4.48|P-value:3.66E-6||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ERP5(YHR110W)|FD-Score:-3.23|P-value:6.20E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FRK1(YPL141C)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:GLO2(YDR272W)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:ISR1(YPR106W)|FD-Score:-3.31|P-value:4.75E-4||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:KAR9(YPL269W)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:KEL2(YGR238C)|FD-Score:-4.96|P-value:3.57E-7||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:KRE2(YDR483W)|FD-Score:3.98|P-value:3.48E-5||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LEA1(YPL213W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:LIP2(YLR239C)|FD-Score:5.02|P-value:2.57E-7||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:LOC1(YFR001W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:MDY2(YOL111C)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MET17(YLR303W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MPC2(YHR162W)|FD-Score:5.4|P-value:3.34E-8||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium Gene:MSA2(YKR077W)|FD-Score:4.39|P-value:5.66E-6||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MTD1(YKR080W)|FD-Score:6.49|P-value:4.27E-11||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:NIT2(YJL126W)|FD-Score:3.77|P-value:8.17E-5||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:OLA1(YBR025C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress Gene:OSH2(YDL019C)|FD-Score:4.38|P-value:5.89E-6||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAF1(YBR279W)|FD-Score:4.75|P-value:9.95E-7||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PAN3(YKL025C)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PEP12(YOR036W)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEX32(YBR168W)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PHR1(YOR386W)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:PRM4(YPL156C)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift Gene:PTC1(YDL006W)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PYC1(YGL062W)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:RAD14(YMR201C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RAD34(YDR314C)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Protein involved in nucleotide excision repair (NER); homologous to RAD4 Gene:RPL16A(YIL133C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RRT2(YBR246W)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:RTS3(YGR161C_p)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SCO2(YBR024W)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication Gene:SFB3(YHR098C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SIP5(YMR140W)|FD-Score:-7.84|P-value:2.18E-15||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SLH1(YGR271W)|FD-Score:4.93|P-value:4.03E-7||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SSO2(YMR183C)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p Gene:TMA23(YMR269W)|FD-Score:4.5|P-value:3.35E-6||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TRM11(YOL124C)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain Gene:TSC3(YBR058C-A)|FD-Score:15.1|P-value:7.74E-52||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TSR2(YLR435W)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:YAL037W(YAL037W_p)|FD-Score:-3.14|P-value:8.33E-4||SGD DESC:Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication Gene:YBL096C(YBL096C_d)|FD-Score:-4.19|P-value:1.40E-5||SGD DESC:Non-essential protein of unknown function Gene:YBR027C(YBR027C_d)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR015C(YCR015C_p)|FD-Score:-3.3|P-value:4.86E-4||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YDL211C(YDL211C_p)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR222W(YDR222W_p)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YDR249C(YDR249C_p)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:Putative protein of unknown function Gene:YER184C(YER184C_p)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source Gene:YHR007C-A(YHR007C-A_p)|FD-Score:-3.19|P-value:7.24E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YHR095W(YHR095W_d)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL055W(YJL055W_p)|FD-Score:5.71|P-value:5.57E-9||SGD DESC:Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU Gene:YJL206C(YJL206C_p)|FD-Score:3.32|P-value:4.55E-4||SGD DESC:Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment Gene:YKL069W(YKL069W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YMR122C(YMR122C_d)|FD-Score:5.64|P-value:8.70E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL143C(YNL143C_p)|FD-Score:4.89|P-value:5.17E-7||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOL097W-A(YOL097W-A_p)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR376W-A(YOR376W-A_p)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPL272C(YPL272C_p)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Putative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene Gene:YPR099C(YPR099C_d)|FD-Score:-3.44|P-value:2.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR236C7.135.10E-131.71ABD1Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA
YOR250C5.422.96E-80.63CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YOR340C4.798.19E-70.67RPA43RNA polymerase I subunit A43
YLR438C-A4.121.87E-50.17LSM3Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YGL169W3.963.80E-50.42SUA5Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family
YLR116W3.542.01E-40.07MSL5Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA
YFR002W3.472.58E-40.04NIC96Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p)
YPL169C3.433.02E-40.06MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YLR275W3.373.75E-40.07SMD2Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
YDR081C3.304.83E-40.11PDC2Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
YOR149C3.197.13E-40.14SMP3Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure
YBR038W3.050.001150.06CHS2Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis
YDL087C2.990.001400.06LUC7Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA
YDR212W2.930.001690.03TCP1Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
YJR123W2.900.001840.10RPS5Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A15.107.74E-52TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YKR080W6.494.27E-11MTD1NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline
YJL055W_p5.715.57E-9YJL055W_pPutative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU
YMR122C_d5.648.70E-9YMR122C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YHR162W5.403.34E-8MPC2Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium
YLR239C5.022.57E-7LIP2Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
YGR271W4.934.03E-7SLH1Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses
YNL143C_p4.895.17E-7YNL143C_pProtein of unknown function; expressed at both mRNA and protein levels
YDL024C4.875.49E-7DIA3Protein of unknown function, involved in invasive and pseudohyphal growth
YBR279W4.759.95E-7PAF1Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1
YPL269W4.592.23E-6KAR9Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase
YMR269W4.503.35E-6TMA23Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan
YGR271C-A4.483.66E-6EFG1Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus
YKR077W4.395.66E-6MSA2Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation
YDL019C4.385.89E-6OSH2Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability

GO enrichment analysis for SGTC_47
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3261.57E-146SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0350877TSC3-RPN4
0.3101.51E-131SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.0704225TSC3-RPN4
0.3066.51E-128SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0952381TSC3-RPN4
0.3012.77E-124SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.103448TSC3-RPN4
0.2892.68E-114SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0517241TSC3-RPN4
0.2866.37E-112SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0634921TSC3-RPN4
0.2653.04E-95SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0701754TSC3-RPN4
0.2611.44E-92SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.0821918TSC3-RPN4
0.2601.22E-91SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0555556TSC3-RPN4
0.2542.55E-87SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0925926TSC3-RPN4
0.2475.54E-83SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.175439TSC3-RPN4
0.2421.89E-79SGTC_1813st051246 8.6 μMTimTec (Natural product derivative library)52919590.0535714TSC3-RPN4
0.2393.30E-77SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.127273TSC3-RPN4
0.2356.33E-75SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.0882353
0.2331.33E-73SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.0714286

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_950471-0001561.06 μM0.6578954015405ChemDiv (Drug-like library)356.455325.75134
SGTC_2764methyldopa94.69 μM0.41463438853Miscellaneous211.21452-1.77845
SGTC_4982,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)150 μM0.3870972374ICCB bioactive library222.323284.14622Golgi
SGTC_1863455-346567.28 μM0.318182755278ChemDiv (Drug-like library)325.144954.00312
SGTC_462ag-879158 μM0.2916676809654ICCB bioactive library316.460963.88224
SGTC_1535tetrahydropapaveroline hydrobromide54.3 μM0.2916672724278TimTec (Pure natural product library)368.222422.89855RPP1 & pyrimidine depletion
SGTC_6961560-0013611 μM0.281650269ChemDiv (Drug-like library)327.4356436.1913
SGTC_2669eugenol100 μM0.2727273314Microsource (Natural product library)164.201082.57912
SGTC_275trichlorophene5.69 μM0.26829362616Miscellaneous409.69037.04633mitochondrial stress
SGTC_2723salbutamol83.58 μM0.2448989884233NIH Clinical Collection337.389220.33468