calcifediol FDA approved compound

(1S,3Z)-3-[(2E)-2-[(1R,3aS,7aR)-1-[(2R)-6-hydroxy-6-methylheptan-2-yl]-7a-methyl-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylidenecyclohexan-1-ol

A vitamin D metabolite.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_472
Screen concentration 10.0 μM
Source ICCB bioactive library
PubChem CID 5283731
SMILES CC(CCCC(C)(C)O)C1CCC2C1(CCCC2=CC=C3CC(CCC3=C)O)C
Standardized SMILES CC(CCCC(C)(C)O)C1CCC2C(=CC=C3CC(O)CCC3=C)CCCC12C
Molecular weight 400.6371
ALogP 6.4
H-bond donor count 2
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5283731
Download HIP data (tab-delimited text)  (excel)
Gene:CDC6(YJL194W)|FD-Score:3.93|P-value:4.24E-5|Clearance:0.52||SGD DESC:Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:NOP53(YPL146C)|FD-Score:3.09|P-value:9.87E-4|Clearance:0.21||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:RNR2(YJL026W)|FD-Score:3.41|P-value:3.29E-4|Clearance:0.13||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPC37(YKR025W)|FD-Score:3.27|P-value:5.31E-4|Clearance:0.18||SGD DESC:RNA polymerase III subunit C37 Gene:CDC6(YJL194W)|FD-Score:3.93|P-value:4.24E-5|Clearance:0.52||SGD DESC:Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:NOP53(YPL146C)|FD-Score:3.09|P-value:9.87E-4|Clearance:0.21||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:RNR2(YJL026W)|FD-Score:3.41|P-value:3.29E-4|Clearance:0.13||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPC37(YKR025W)|FD-Score:3.27|P-value:5.31E-4|Clearance:0.18||SGD DESC:RNA polymerase III subunit C37

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5283731
Download HOP data (tab-delimited text)  (excel)
Gene:AFG1(YEL052W)|FD-Score:-3.11|P-value:9.20E-4||SGD DESC:Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain Gene:BTS1(YPL069C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CRN1(YLR429W)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly Gene:DAS1(YJL149W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DER1(YBR201W)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p Gene:DIN7(YDR263C)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:GZF3(YJL110C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:LTP1(YPR073C)|FD-Score:-4.46|P-value:4.15E-6||SGD DESC:Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine Gene:MCA1(YOR197W)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization Gene:MNN10(YDR245W)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:NPT1(YOR209C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:OAF1(YAL051W)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:OCA4(YCR095C_p)|FD-Score:3.82|P-value:6.65E-5||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:PPZ1(YML016C)|FD-Score:3.83|P-value:6.30E-5||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:RPL18B(YNL301C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:SAS4(YDR181C)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SLK19(YOR195W)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate Gene:TFS1(YLR178C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Protein that interacts with and inhibits carboxypeptidase Y and Ira2p; phosphatidylethanolamine-binding protein (PEBP) family member; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase; protein abundance increases in response to DNA replication stress Gene:TRM3(YDL112W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:ULA1(YPL003W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation Gene:VPS69(YPR087W_d)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:VPS74(YDR372C)|FD-Score:-3.88|P-value:5.24E-5||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:VTA1(YLR181C)|FD-Score:4.28|P-value:9.49E-6||SGD DESC:Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain Gene:WSC4(YHL028W)|FD-Score:4.37|P-value:6.29E-6||SGD DESC:ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 Gene:YDR061W(YDR061W_p)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance Gene:YDR426C(YDR426C_d)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 Gene:YDR445C(YDR445C_d)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR007C-A(YHR007C-A_p)|FD-Score:-3.98|P-value:3.39E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL016W(YJL016W_p)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YJL027C(YJL027C_p)|FD-Score:5.54|P-value:1.53E-8||SGD DESC:Putative protein of unknown function Gene:YJR003C(YJR003C_p)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis Gene:YKR104W(YKR104W)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLR041W(YLR041W_d)|FD-Score:-3.22|P-value:6.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YLR040C Gene:YOR034C-A(YOR034C-A_p)|FD-Score:-3.8|P-value:7.25E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:AFG1(YEL052W)|FD-Score:-3.11|P-value:9.20E-4||SGD DESC:Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain Gene:BTS1(YPL069C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CRN1(YLR429W)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly Gene:DAS1(YJL149W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DER1(YBR201W)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p Gene:DIN7(YDR263C)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:GZF3(YJL110C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:LTP1(YPR073C)|FD-Score:-4.46|P-value:4.15E-6||SGD DESC:Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine Gene:MCA1(YOR197W)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization Gene:MNN10(YDR245W)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:NPT1(YOR209C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:OAF1(YAL051W)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:OCA4(YCR095C_p)|FD-Score:3.82|P-value:6.65E-5||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:PPZ1(YML016C)|FD-Score:3.83|P-value:6.30E-5||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:RPL18B(YNL301C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:SAS4(YDR181C)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SLK19(YOR195W)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate Gene:TFS1(YLR178C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Protein that interacts with and inhibits carboxypeptidase Y and Ira2p; phosphatidylethanolamine-binding protein (PEBP) family member; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase; protein abundance increases in response to DNA replication stress Gene:TRM3(YDL112W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:ULA1(YPL003W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation Gene:VPS69(YPR087W_d)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:VPS74(YDR372C)|FD-Score:-3.88|P-value:5.24E-5||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:VTA1(YLR181C)|FD-Score:4.28|P-value:9.49E-6||SGD DESC:Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain Gene:WSC4(YHL028W)|FD-Score:4.37|P-value:6.29E-6||SGD DESC:ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 Gene:YDR061W(YDR061W_p)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance Gene:YDR426C(YDR426C_d)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 Gene:YDR445C(YDR445C_d)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR007C-A(YHR007C-A_p)|FD-Score:-3.98|P-value:3.39E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL016W(YJL016W_p)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YJL027C(YJL027C_p)|FD-Score:5.54|P-value:1.53E-8||SGD DESC:Putative protein of unknown function Gene:YJR003C(YJR003C_p)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis Gene:YKR104W(YKR104W)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLR041W(YLR041W_d)|FD-Score:-3.22|P-value:6.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YLR040C Gene:YOR034C-A(YOR034C-A_p)|FD-Score:-3.8|P-value:7.25E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL194W3.934.24E-50.52CDC6Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress
YJL026W3.413.29E-40.13RNR2Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits
YKR025W3.275.31E-40.18RPC37RNA polymerase III subunit C37
YPL146C3.099.87E-40.21NOP53Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired
YBR265W2.880.001990.02TSC103-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family
YOR319W2.860.002100.07HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YHR069C2.790.002610.12RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YNR054C2.670.003780.02ESF2Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome
YDL208W2.650.003990.01NHP2Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing
YPL169C2.640.004100.02MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YPR133C2.630.004300.13SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YLR167W2.500.006190.03RPS31Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
YKL012W2.470.006790.01PRP40U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex
YGL128C2.460.006930.01CWC23Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p
YOL022C2.450.007071.35E-4TSR4Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL027C_p5.541.53E-8YJL027C_pPutative protein of unknown function
YHL028W4.376.29E-6WSC4ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3
YLR181C4.289.49E-6VTA1Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain
YDR263C4.022.96E-5DIN7Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination
YML016C3.836.30E-5PPZ1Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance
YCR095C_p3.826.65E-5OCA4_pCytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts
YJL110C3.721.00E-4GZF3GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication
YPL069C3.701.08E-4BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YDR445C_d3.701.10E-4YDR445C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDL112W3.671.23E-4TRM32'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs
YDR181C3.591.62E-4SAS4Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p
YOR209C3.551.96E-4NPT1Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus
YJL149W3.492.38E-4DAS1Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C
YOR197W3.432.98E-4MCA1Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization
YLR178C3.423.08E-4TFS1Protein that interacts with and inhibits carboxypeptidase Y and Ira2p; phosphatidylethanolamine-binding protein (PEBP) family member; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_472
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1201.81E-20SGTC_273hydroxyurea 18.1 mMMiscellaneous36570.0149254heme biosynthesis & mitochondrial translocase
0.0857.62E-11SGTC_482fipronil 114.0 μMICCB bioactive library33520.0204082
0.0723.73E-8SGTC_1644st010134 20.9 μMTimTec (Natural product derivative library)38181340.0689655mitochondrial processes
0.0649.00E-7SGTC_8620452-0056 10.2 μMChemDiv (Drug-like library)15753960.0454545azole & statin
0.0622.12E-6SGTC_1752st044819 83.0 μMTimTec (Natural product derivative library)4412090.0617284
0.0604.02E-6SGTC_1608st001473 40.5 μMTimTec (Natural product derivative library)72922670.0243902
0.0587.07E-6SGTC_1892st058411 7.4 μMTimTec (Natural product derivative library)39347380.0322581
0.0587.51E-6SGTC_1764st044502 93.5 μMTimTec (Natural product derivative library)84070.0123457
0.0571.40E-5SGTC_1607st000305 50.6 μMTimTec (Natural product derivative library)54576900.0392157
0.0552.34E-5SGTC_917harmaline 728.1 nMTimTec (Natural product library)52809510.0449438ergosterol biosynthesis
0.0542.90E-5SGTC_1121fusaric acid 630.5 nMTimTec (Natural product library)34420.0609756
0.0535.30E-5SGTC_2961222-0039 74.9 μMChemDiv (Drug-like library)59841760.0731707
0.0518.33E-5SGTC_6041000-0114 314.0 μMChemDiv (Drug-like library)15727450.0444444
0.0519.47E-5SGTC_4732-arachidonoylglycerol 10.0 μMICCB bioactive library52822800.0705882
0.0501.39E-4SGTC_2575plumbagin 510.0 nMTimTec (Pure natural product library)102050.037037superoxide

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2642cholecalciferol100 μM0.8032799821465Microsource (Natural product library)384.637667.75511
SGTC_2628lathosterol100 μM0.3333336710616Microsource (Natural product library)386.653547.37611
SGTC_2537larixol68.93 μM0.2658236708759Microsource (Natural product library)306.482764.12122
SGTC_2539epi(13)torulosol80 μM0.2592596708597Microsource (Natural product library)306.482764.19922
SGTC_1560lithocholic acid53.1 μM0.2409649903TimTec (Pure natural product library)376.57265.18423
SGTC_2687chenodiol100 μM0.2380955702181Microsource (Natural product library)392.5724.01434
SGTC_2625euphol100 μM0.2111116708538Microsource (Natural product library)426.71748.11911
SGTC_1517deoxycholic acid methyl ester49.2 μM0.208791229346TimTec (Pure natural product library)406.598584.30724
SGTC_2531methyl deoxycholate70.07 μM0.20879116667744Microsource (Natural product library)406.598584.30724
SGTC_2621lithochol-11-enic acid100 μM0.2087916708711Microsource (Natural product library)374.556724.73923