2-arachidonoylglycerol

1,3-dihydroxypropan-2-yl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate

An endocannabinoid.

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PubChem MeSH terms: Endocannabinoids



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_473
Screen concentration 10.0 μM
Source ICCB bioactive library
PubChem CID 5282280
SMILES CCCCCC=CCC=CCC=CCC=CCCCC(=O)OC(CO)CO
Standardized SMILES CCCCCC=CCC=CCC=CCC=CCCCC(=O)OC(CO)CO
Molecular weight 378.5454
ALogP 5.61
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.8
% growth inhibition (Hom. pool) 1.44


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5282280
Download HIP data (tab-delimited text)  (excel)
Gene:ABD1(YBR236C)|FD-Score:3.68|P-value:1.14E-4|Clearance:0.08||SGD DESC:Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA Gene:APC2(YLR127C)|FD-Score:4.02|P-value:2.88E-5|Clearance:0.03||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p Gene:CCT5(YJR064W)|FD-Score:-3.26|P-value:5.61E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:DRE2(YKR071C)|FD-Score:-3.54|P-value:2.02E-4|Clearance:0||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:DRS1(YLL008W)|FD-Score:3.56|P-value:1.88E-4|Clearance:0.13||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:DUO1(YGL061C)|FD-Score:-3.57|P-value:1.77E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ERG8(YMR220W)|FD-Score:-3.59|P-value:1.62E-4|Clearance:0||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:FRQ1(YDR373W)|FD-Score:-3.51|P-value:2.23E-4|Clearance:0||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:KRS1(YDR037W)|FD-Score:3.99|P-value:3.28E-5|Clearance:0.14||SGD DESC:Lysyl-tRNA synthetase Gene:MET4(YNL103W)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.05||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:MSL5(YLR116W)|FD-Score:-3.79|P-value:7.52E-5|Clearance:0||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:NSE3(YDR288W)|FD-Score:-3.33|P-value:4.33E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:PIS1(YPR113W)|FD-Score:-3.91|P-value:4.56E-5|Clearance:0||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:RLP24(YLR009W)|FD-Score:3.42|P-value:3.09E-4|Clearance:0.12||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:ROT1(YMR200W)|FD-Score:3.18|P-value:7.25E-4|Clearance:0.03||SGD DESC:Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation Gene:RPN11(YFR004W)|FD-Score:3.15|P-value:8.05E-4|Clearance:0.12||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:SAS10(YDL153C)|FD-Score:3.22|P-value:6.47E-4|Clearance:0.03||SGD DESC:Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance Gene:SEC13(YLR208W)|FD-Score:3.85|P-value:5.81E-5|Clearance:0.17||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SPC29(YPL124W)|FD-Score:-4.32|P-value:7.77E-6|Clearance:0||SGD DESC:Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication Gene:TEM1(YML064C)|FD-Score:3.3|P-value:4.80E-4|Clearance:0.07||SGD DESC:GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis Gene:UFE1(YOR075W)|FD-Score:4.16|P-value:1.58E-5|Clearance:0.14||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER Gene:UTP11(YKL099C)|FD-Score:4.51|P-value:3.30E-6|Clearance:0.35||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YML6(YML025C)|FD-Score:3.23|P-value:6.10E-4|Clearance:0.02||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins Gene:ABD1(YBR236C)|FD-Score:3.68|P-value:1.14E-4|Clearance:0.08||SGD DESC:Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA Gene:APC2(YLR127C)|FD-Score:4.02|P-value:2.88E-5|Clearance:0.03||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p Gene:CCT5(YJR064W)|FD-Score:-3.26|P-value:5.61E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:DRE2(YKR071C)|FD-Score:-3.54|P-value:2.02E-4|Clearance:0||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:DRS1(YLL008W)|FD-Score:3.56|P-value:1.88E-4|Clearance:0.13||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:DUO1(YGL061C)|FD-Score:-3.57|P-value:1.77E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ERG8(YMR220W)|FD-Score:-3.59|P-value:1.62E-4|Clearance:0||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:FRQ1(YDR373W)|FD-Score:-3.51|P-value:2.23E-4|Clearance:0||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:KRS1(YDR037W)|FD-Score:3.99|P-value:3.28E-5|Clearance:0.14||SGD DESC:Lysyl-tRNA synthetase Gene:MET4(YNL103W)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.05||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:MSL5(YLR116W)|FD-Score:-3.79|P-value:7.52E-5|Clearance:0||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:NSE3(YDR288W)|FD-Score:-3.33|P-value:4.33E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:PIS1(YPR113W)|FD-Score:-3.91|P-value:4.56E-5|Clearance:0||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:RLP24(YLR009W)|FD-Score:3.42|P-value:3.09E-4|Clearance:0.12||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:ROT1(YMR200W)|FD-Score:3.18|P-value:7.25E-4|Clearance:0.03||SGD DESC:Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation Gene:RPN11(YFR004W)|FD-Score:3.15|P-value:8.05E-4|Clearance:0.12||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:SAS10(YDL153C)|FD-Score:3.22|P-value:6.47E-4|Clearance:0.03||SGD DESC:Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance Gene:SEC13(YLR208W)|FD-Score:3.85|P-value:5.81E-5|Clearance:0.17||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SPC29(YPL124W)|FD-Score:-4.32|P-value:7.77E-6|Clearance:0||SGD DESC:Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication Gene:TEM1(YML064C)|FD-Score:3.3|P-value:4.80E-4|Clearance:0.07||SGD DESC:GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis Gene:UFE1(YOR075W)|FD-Score:4.16|P-value:1.58E-5|Clearance:0.14||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER Gene:UTP11(YKL099C)|FD-Score:4.51|P-value:3.30E-6|Clearance:0.35||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YML6(YML025C)|FD-Score:3.23|P-value:6.10E-4|Clearance:0.02||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5282280
Download HOP data (tab-delimited text)  (excel)
Gene:AMN1(YBR158W)|FD-Score:-4.35|P-value:6.96E-6||SGD DESC:Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) Gene:ARN2(YHL047C)|FD-Score:4.94|P-value:3.85E-7||SGD DESC:Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C Gene:ARO1(YDR127W)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATC1(YDR184C)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:BCH2(YKR027W)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BNI1(YNL271C)|FD-Score:-5.25|P-value:7.73E-8||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:CIT1(YNR001C)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:CMC1(YKL137W)|FD-Score:-3.83|P-value:6.31E-5||SGD DESC:Evolutionarily conserved copper-binding protein of the mitochondrial intermembrane space, may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif Gene:COS1(YNL336W)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:DIN7(YDR263C)|FD-Score:8.55|P-value:6.07E-18||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:EDC1(YGL222C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress Gene:GCN2(YDR283C)|FD-Score:6.76|P-value:7.03E-12||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:4.52|P-value:3.11E-6||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GDE1(YPL110C)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes Gene:GGA1(YDR358W)|FD-Score:-4.34|P-value:7.08E-6||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GLR1(YPL091W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GPT2(YKR067W)|FD-Score:4.57|P-value:2.50E-6||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:GRX8(YLR364W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Glutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic Gene:HCH1(YNL281W)|FD-Score:3.88|P-value:5.29E-5||SGD DESC:Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:HOP2(YGL033W)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:IDP3(YNL009W)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication Gene:IST1(YNL265C)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:MCT1(YOR221C)|FD-Score:-4.72|P-value:1.16E-6||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MON1(YGL124C)|FD-Score:-3.86|P-value:5.69E-5||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRPL37(YBR268W)|FD-Score:-3.19|P-value:7.03E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NAS6(YGR232W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6 Gene:PCA1(YBR295W)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function Gene:PDR8(YLR266C)|FD-Score:-3.12|P-value:8.90E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PET494(YNR045W)|FD-Score:-4.6|P-value:2.11E-6||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane Gene:PEX13(YLR191W)|FD-Score:-3.8|P-value:7.22E-5||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PEX5(YDR244W)|FD-Score:-4.65|P-value:1.64E-6||SGD DESC:Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions Gene:PIF1(YML061C)|FD-Score:-3.79|P-value:7.65E-5||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:RAD17(YOR368W)|FD-Score:3.78|P-value:7.89E-5||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RAD33(YML011C)|FD-Score:4.29|P-value:8.86E-6||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:ROM1(YGR070W)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPL17B(YJL177W)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:RPS16B(YDL083C)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:RSF1(YMR030W)|FD-Score:-3.88|P-value:5.13E-5||SGD DESC:Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes Gene:RSR1(YGR152C)|FD-Score:-3.12|P-value:8.92E-4||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:SOD1(YJR104C)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPE2(YOL052C)|FD-Score:-3.09|P-value:9.92E-4||SGD DESC:S-adenosylmethionine decarboxylase, required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically Gene:SPT2(YER161C)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:STP3(YLR375W)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:SWE1(YJL187C)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate Gene:SWP82(YFL049W)|FD-Score:4.53|P-value:2.98E-6||SGD DESC:Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions Gene:TAN1(YGL232W)|FD-Score:-4.53|P-value:2.88E-6||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TGL2(YDR058C)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TRM3(YDL112W)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:TRM7(YBR061C)|FD-Score:-3.34|P-value:4.26E-4||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TRP1(YDR007W)|FD-Score:3.12|P-value:8.89E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP3(YKL211C)|FD-Score:5.71|P-value:5.66E-9||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:5.08|P-value:1.92E-7||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSA1(YML028W)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:VHS2(YIL135C)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p Gene:YCL042W(YCL042W_p)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YCR007C(YCR007C_p)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDL034W(YDL034W_d)|FD-Score:4.49|P-value:3.54E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene Gene:YDL050C(YDL050C_d)|FD-Score:-3.17|P-value:7.59E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL177C(YDL177C_p)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:4.65|P-value:1.62E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR230W(YDR230W_d)|FD-Score:-4.41|P-value:5.28E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20 Gene:YGR042W(YGR042W_p)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress Gene:YGR269W(YGR269W_d)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YGR273C(YGR273C_p)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHR050W-A(YHR050W-A_p)|FD-Score:4.41|P-value:5.08E-6||SGD DESC:Protein of unknown function; identified by expression profiling and mass spectrometry Gene:YHR127W(YHR127W)|FD-Score:-3.24|P-value:6.05E-4||SGD DESC:Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis Gene:YKL075C(YKL075C_p)|FD-Score:-3.94|P-value:4.12E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YLL059C(YLL059C_d)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML094C-A(YML094C-A_d)|FD-Score:7.49|P-value:3.35E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YMR057C(YMR057C_d)|FD-Score:4.72|P-value:1.18E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YMR099C(YMR099C)|FD-Score:5.79|P-value:3.60E-9||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YNL043C(YNL043C_d)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YNL050C(YNL050C_p)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YNL092W(YNL092W_p)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene Gene:YNL203C(YNL203C_d)|FD-Score:-3.93|P-value:4.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL303W(YNL303W_d)|FD-Score:4.82|P-value:7.15E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL037C(YOL037C_d)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Gene:YOR302W(YOR302W)|FD-Score:4.54|P-value:2.86E-6||SGD DESC:CPA1 uORF, Arginine attenuator peptide, regulates translation of the CPA1 mRNA Gene:YOR376W-A(YOR376W-A_p)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR117W(YPR117W_p)|FD-Score:-5.11|P-value:1.65E-7||SGD DESC:Putative protein of unknown function Gene:AMN1(YBR158W)|FD-Score:-4.35|P-value:6.96E-6||SGD DESC:Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) Gene:ARN2(YHL047C)|FD-Score:4.94|P-value:3.85E-7||SGD DESC:Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C Gene:ARO1(YDR127W)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATC1(YDR184C)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:BCH2(YKR027W)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BNI1(YNL271C)|FD-Score:-5.25|P-value:7.73E-8||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:CIT1(YNR001C)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:CMC1(YKL137W)|FD-Score:-3.83|P-value:6.31E-5||SGD DESC:Evolutionarily conserved copper-binding protein of the mitochondrial intermembrane space, may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif Gene:COS1(YNL336W)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:DIN7(YDR263C)|FD-Score:8.55|P-value:6.07E-18||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:EDC1(YGL222C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress Gene:GCN2(YDR283C)|FD-Score:6.76|P-value:7.03E-12||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:4.52|P-value:3.11E-6||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GDE1(YPL110C)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes Gene:GGA1(YDR358W)|FD-Score:-4.34|P-value:7.08E-6||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GLR1(YPL091W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GPT2(YKR067W)|FD-Score:4.57|P-value:2.50E-6||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:GRX8(YLR364W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Glutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic Gene:HCH1(YNL281W)|FD-Score:3.88|P-value:5.29E-5||SGD DESC:Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:HOP2(YGL033W)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:IDP3(YNL009W)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication Gene:IST1(YNL265C)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:MCT1(YOR221C)|FD-Score:-4.72|P-value:1.16E-6||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MON1(YGL124C)|FD-Score:-3.86|P-value:5.69E-5||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRPL37(YBR268W)|FD-Score:-3.19|P-value:7.03E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NAS6(YGR232W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6 Gene:PCA1(YBR295W)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function Gene:PDR8(YLR266C)|FD-Score:-3.12|P-value:8.90E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PET494(YNR045W)|FD-Score:-4.6|P-value:2.11E-6||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane Gene:PEX13(YLR191W)|FD-Score:-3.8|P-value:7.22E-5||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PEX5(YDR244W)|FD-Score:-4.65|P-value:1.64E-6||SGD DESC:Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions Gene:PIF1(YML061C)|FD-Score:-3.79|P-value:7.65E-5||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:RAD17(YOR368W)|FD-Score:3.78|P-value:7.89E-5||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RAD33(YML011C)|FD-Score:4.29|P-value:8.86E-6||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:ROM1(YGR070W)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPL17B(YJL177W)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:RPS16B(YDL083C)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:RSF1(YMR030W)|FD-Score:-3.88|P-value:5.13E-5||SGD DESC:Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes Gene:RSR1(YGR152C)|FD-Score:-3.12|P-value:8.92E-4||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:SOD1(YJR104C)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPE2(YOL052C)|FD-Score:-3.09|P-value:9.92E-4||SGD DESC:S-adenosylmethionine decarboxylase, required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically Gene:SPT2(YER161C)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:STP3(YLR375W)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:SWE1(YJL187C)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate Gene:SWP82(YFL049W)|FD-Score:4.53|P-value:2.98E-6||SGD DESC:Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions Gene:TAN1(YGL232W)|FD-Score:-4.53|P-value:2.88E-6||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TGL2(YDR058C)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TRM3(YDL112W)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:TRM7(YBR061C)|FD-Score:-3.34|P-value:4.26E-4||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TRP1(YDR007W)|FD-Score:3.12|P-value:8.89E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP3(YKL211C)|FD-Score:5.71|P-value:5.66E-9||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:5.08|P-value:1.92E-7||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSA1(YML028W)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:VHS2(YIL135C)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p Gene:YCL042W(YCL042W_p)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YCR007C(YCR007C_p)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDL034W(YDL034W_d)|FD-Score:4.49|P-value:3.54E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene Gene:YDL050C(YDL050C_d)|FD-Score:-3.17|P-value:7.59E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL177C(YDL177C_p)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:4.65|P-value:1.62E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR230W(YDR230W_d)|FD-Score:-4.41|P-value:5.28E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20 Gene:YGR042W(YGR042W_p)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress Gene:YGR269W(YGR269W_d)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YGR273C(YGR273C_p)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHR050W-A(YHR050W-A_p)|FD-Score:4.41|P-value:5.08E-6||SGD DESC:Protein of unknown function; identified by expression profiling and mass spectrometry Gene:YHR127W(YHR127W)|FD-Score:-3.24|P-value:6.05E-4||SGD DESC:Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis Gene:YKL075C(YKL075C_p)|FD-Score:-3.94|P-value:4.12E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YLL059C(YLL059C_d)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML094C-A(YML094C-A_d)|FD-Score:7.49|P-value:3.35E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YMR057C(YMR057C_d)|FD-Score:4.72|P-value:1.18E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YMR099C(YMR099C)|FD-Score:5.79|P-value:3.60E-9||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YNL043C(YNL043C_d)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YNL050C(YNL050C_p)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YNL092W(YNL092W_p)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene Gene:YNL203C(YNL203C_d)|FD-Score:-3.93|P-value:4.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL303W(YNL303W_d)|FD-Score:4.82|P-value:7.15E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL037C(YOL037C_d)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Gene:YOR302W(YOR302W)|FD-Score:4.54|P-value:2.86E-6||SGD DESC:CPA1 uORF, Arginine attenuator peptide, regulates translation of the CPA1 mRNA Gene:YOR376W-A(YOR376W-A_p)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR117W(YPR117W_p)|FD-Score:-5.11|P-value:1.65E-7||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL099C4.513.30E-60.34UTP11Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YOR075W4.161.58E-50.14UFE1t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER
YLR127C4.022.88E-50.03APC2Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p
YDR037W3.993.28E-50.14KRS1Lysyl-tRNA synthetase
YLR208W3.855.81E-50.17SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YBR236C3.681.14E-40.08ABD1Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA
YNL103W3.611.55E-40.05MET4Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p
YLL008W3.561.88E-40.13DRS1Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles
YLR009W3.423.09E-40.12RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YML064C3.304.80E-40.07TEM1GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis
YML025C3.236.10E-40.02YML6Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins
YDL153C3.226.47E-40.03SAS10Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance
YMR200W3.187.25E-40.03ROT1Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation
YFR004W3.158.05E-40.12RPN11Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
YFL017C3.030.001210.01GNA1Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR263C8.556.07E-18DIN7Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination
YML094C-A_d7.493.35E-14YML094C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO
YDR283C6.767.03E-12GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YMR099C5.793.60E-9YMR099CGlucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS
YKL211C5.715.66E-9TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR354W5.081.92E-7TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YHL047C4.943.85E-7ARN2Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C
YNL303W_d4.827.15E-7YNL303W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YMR057C_d4.721.18E-6YMR057C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1
YDR008C_d4.651.62E-6YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YKR027W4.631.86E-6BCH2Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication
YKR067W4.572.50E-6GPT2Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen
YOR302W4.542.86E-6YOR302WCPA1 uORF, Arginine attenuator peptide, regulates translation of the CPA1 mRNA
YFL049W4.532.98E-6SWP82Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions
YFR009W4.523.11E-6GCN20Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA

GO enrichment analysis for SGTC_473
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2273.12E-69SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.12
0.2152.45E-62SGTC_2616abietic acid 100.0 μMMicrosource (Natural product library)67086780.0821918
0.2098.11E-59SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0434783
0.1993.76E-53SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.0224719
0.1952.68E-51SGTC_6770929-0063 136.0 μMChemDiv (Drug-like library)15395830.0625
0.1913.87E-49SGTC_20384100038 31.0 μMChembridge (Fragment library)3596803NAplasma membrane duress
0.1908.51E-49SGTC_7234182-0001 115.0 μMChemDiv (Drug-like library)7448210.0793651
0.1895.37E-48SGTC_33089128332 17.6 μMChembridge (Drug-like library)173328050.0131579plasma membrane duress
0.1874.19E-47SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.272727
0.1852.83E-46SGTC_24785763493 32.3 μMMiscellaneous13655760.0597015
0.1814.12E-44SGTC_8191013-0258 27.7 μMChemDiv (Drug-like library)57531980.0657895
0.1793.27E-43SGTC_24885268135 45.7 μMMiscellaneous12703560.03125
0.1785.97E-43SGTC_505gw-5074 63.9 μMICCB bioactive library59242080.0434783
0.1781.14E-42SGTC_14964469-0713 57.4 μMChemDiv (Drug-like library)7466910.0540541
0.1771.71E-42SGTC_465cgp-37157 41.0 μMICCB bioactive library26880.0140845

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_409arachidonic acid100 μM0.578947231Miscellaneous304.466886.43912
SGTC_476mead acid10 μM0.5641035312531ICCB bioactive library306.482766.88312
SGTC_477mead ethanolamide10 μM0.51111116061185ICCB bioactive library349.550565.92222
SGTC_478N-arachidonylglycine10 μM0.4888895283389ICCB bioactive library361.51825.49923
SGTC_479n-linoleoylglycine10 μM0.4468096433346ICCB bioactive library337.49685.47623
SGTC_410α-linolenic acid100 μM0.418605860Miscellaneous278.42965.97112excess fatty acid
SGTC_475α-linolenic acid10 μM0.4186055280934ICCB bioactive library278.42965.97112excess fatty acid
SGTC_1876myriocin605 nM0.350877301119Miscellaneous401.537460.28457
SGTC_438sphingosine6.67 μM0.3478265280335ICCB bioactive library299.491884.82433
SGTC_436n,n-dimethylsphingosine3.3 μM0.3333335282309ICCB bioactive library327.545045.79223
SGTC_1573oleylamine1.06 μM0.3255356789TimTec (Pure natural product library)267.493086.62311