dl-dihydrosphingosine

(2S,3S)-2-aminooctadecane-1,3-diol

A sphingolipid protein kinase C inhibitor.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 

PubChem MeSH terms: Enzyme Inhibitors



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_474
Screen concentration 5.0 μM
Source ICCB bioactive library
PubChem CID 3058739
SMILES CCCCCCCCCCCCCCCC(C(CO)N)O
Standardized SMILES CCCCCCCCCCCCCCCC(O)C(N)CO
Molecular weight 301.5078
ALogP 5.13
H-bond donor count 3
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.8
% growth inhibition (Hom. pool) 5.47


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3058739
Download HIP data (tab-delimited text)  (excel)
Gene:ARP9(YMR033W)|FD-Score:3.8|P-value:7.13E-5|Clearance:0.02||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC123(YLR215C)|FD-Score:-3.36|P-value:3.84E-4|Clearance:0||SGD DESC:Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein Gene:CLP1(YOR250C)|FD-Score:4.84|P-value:6.34E-7|Clearance:0.72||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:CSE1(YGL238W)|FD-Score:-3.12|P-value:8.89E-4|Clearance:0||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:DED81(YHR019C)|FD-Score:4.52|P-value:3.03E-6|Clearance:0.72||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:EFB1(YAL003W)|FD-Score:3.44|P-value:2.96E-4|Clearance:0.05||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:ERG7(YHR072W)|FD-Score:3.67|P-value:1.21E-4|Clearance:0.14||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:GLC7(YER133W)|FD-Score:-3.37|P-value:3.72E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPN2(YOR262W)|FD-Score:3.79|P-value:7.64E-5|Clearance:0.09||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:KAR2(YJL034W)|FD-Score:3.39|P-value:3.55E-4|Clearance:0.18||SGD DESC:ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p Gene:NAM9(YNL137C)|FD-Score:3.47|P-value:2.65E-4|Clearance:0.02||SGD DESC:Mitochondrial ribosomal component of the small subunit Gene:NOP19(YGR251W)|FD-Score:3.53|P-value:2.11E-4|Clearance:0.02||SGD DESC:Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes Gene:PTI1(YGR156W)|FD-Score:-3.43|P-value:2.99E-4|Clearance:0||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RPT2(YDL007W)|FD-Score:3.2|P-value:6.81E-4|Clearance:0.07||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle Gene:RRP46(YGR095C)|FD-Score:3.11|P-value:9.38E-4|Clearance:0.12||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RRP7(YCL031C)|FD-Score:3.21|P-value:6.69E-4|Clearance:0.01||SGD DESC:Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress Gene:RSE1(YML049C)|FD-Score:5.05|P-value:2.19E-7|Clearance:0.72||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:SCL1(YGL011C)|FD-Score:3.7|P-value:1.07E-4|Clearance:0.03||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SEC21(YNL287W)|FD-Score:-4.15|P-value:1.70E-5|Clearance:0||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SPC19(YDR201W)|FD-Score:3.5|P-value:2.32E-4|Clearance:0.04||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:SPT5(YML010W)|FD-Score:3.45|P-value:2.84E-4|Clearance:0.01||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:YGR115C(YGR115C_d)|FD-Score:5.71|P-value:5.59E-9|Clearance:0.72||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YJL015C(YJL015C_d)|FD-Score:-3.64|P-value:1.34E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W Gene:YLR076C(YLR076C_d)|FD-Score:-3.24|P-value:5.96E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YLR101C(YLR101C_d)|FD-Score:3.13|P-value:8.63E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W Gene:ARP9(YMR033W)|FD-Score:3.8|P-value:7.13E-5|Clearance:0.02||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC123(YLR215C)|FD-Score:-3.36|P-value:3.84E-4|Clearance:0||SGD DESC:Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein Gene:CLP1(YOR250C)|FD-Score:4.84|P-value:6.34E-7|Clearance:0.72||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:CSE1(YGL238W)|FD-Score:-3.12|P-value:8.89E-4|Clearance:0||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:DED81(YHR019C)|FD-Score:4.52|P-value:3.03E-6|Clearance:0.72||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:EFB1(YAL003W)|FD-Score:3.44|P-value:2.96E-4|Clearance:0.05||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:ERG7(YHR072W)|FD-Score:3.67|P-value:1.21E-4|Clearance:0.14||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:GLC7(YER133W)|FD-Score:-3.37|P-value:3.72E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPN2(YOR262W)|FD-Score:3.79|P-value:7.64E-5|Clearance:0.09||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:KAR2(YJL034W)|FD-Score:3.39|P-value:3.55E-4|Clearance:0.18||SGD DESC:ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p Gene:NAM9(YNL137C)|FD-Score:3.47|P-value:2.65E-4|Clearance:0.02||SGD DESC:Mitochondrial ribosomal component of the small subunit Gene:NOP19(YGR251W)|FD-Score:3.53|P-value:2.11E-4|Clearance:0.02||SGD DESC:Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes Gene:PTI1(YGR156W)|FD-Score:-3.43|P-value:2.99E-4|Clearance:0||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RPT2(YDL007W)|FD-Score:3.2|P-value:6.81E-4|Clearance:0.07||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle Gene:RRP46(YGR095C)|FD-Score:3.11|P-value:9.38E-4|Clearance:0.12||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RRP7(YCL031C)|FD-Score:3.21|P-value:6.69E-4|Clearance:0.01||SGD DESC:Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress Gene:RSE1(YML049C)|FD-Score:5.05|P-value:2.19E-7|Clearance:0.72||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:SCL1(YGL011C)|FD-Score:3.7|P-value:1.07E-4|Clearance:0.03||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SEC21(YNL287W)|FD-Score:-4.15|P-value:1.70E-5|Clearance:0||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SPC19(YDR201W)|FD-Score:3.5|P-value:2.32E-4|Clearance:0.04||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:SPT5(YML010W)|FD-Score:3.45|P-value:2.84E-4|Clearance:0.01||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:YGR115C(YGR115C_d)|FD-Score:5.71|P-value:5.59E-9|Clearance:0.72||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YJL015C(YJL015C_d)|FD-Score:-3.64|P-value:1.34E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W Gene:YLR076C(YLR076C_d)|FD-Score:-3.24|P-value:5.96E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YLR101C(YLR101C_d)|FD-Score:3.13|P-value:8.63E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3058739
Download HOP data (tab-delimited text)  (excel)
Gene:AIF1(YNR074C)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase Gene:AIM22(YJL046W)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss Gene:ANS1(YHR126C_p)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Putative GPI protein; transcription dependent upon Azf1p Gene:ARO1(YDR127W)|FD-Score:15.3|P-value:2.10E-53||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARP5(YNL059C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ASH1(YKL185W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate Gene:ASM4(YDL088C)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:AZR1(YGR224W)|FD-Score:6.25|P-value:2.10E-10||SGD DESC:Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole Gene:BCH1(YMR237W)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BDF1(YLR399C)|FD-Score:6.06|P-value:6.96E-10||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BDH1(YAL060W)|FD-Score:4|P-value:3.13E-5||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:BIO3(YNR058W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CCP1(YKR066C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CMC4(YMR194C-B)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs Gene:CRH1(YGR189C)|FD-Score:-4.02|P-value:2.97E-5||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:CYS3(YAL012W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:CYS4(YGR155W)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAL81(YIR023W)|FD-Score:6.48|P-value:4.45E-11||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCI1(YOR180C)|FD-Score:4.31|P-value:8.26E-6||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DFG5(YMR238W)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DGA1(YOR245C)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles Gene:DSF2(YBR007C_p)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DUG1(YFR044C)|FD-Score:4.36|P-value:6.45E-6||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:DUS4(YLR405W)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:ERG4(YGL012W)|FD-Score:-3.22|P-value:6.43E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FUB1(YCR076C)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene Gene:GCN4(YEL009C)|FD-Score:6.65|P-value:1.51E-11||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCV1(YDR019C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm Gene:GPR1(YDL035C)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HAP5(YOR358W)|FD-Score:3.74|P-value:9.34E-5||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex Gene:HNT3(YOR258W)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:JEM1(YJL073W)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KAP114(YGL241W)|FD-Score:4.28|P-value:9.47E-6||SGD DESC:Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress Gene:KEL2(YGR238C)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LHS1(YKL073W)|FD-Score:-3.32|P-value:4.45E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MRP2(YPR166C)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL44(YMR225C)|FD-Score:-3.22|P-value:6.40E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MTC3(YGL226W)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:NBA1(YOL070C)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:NHP6A(YPR052C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:OPI3(YJR073C)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PAU11(YGL261C_p)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PAU24(YBR301W)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PCS60(YBR222C)|FD-Score:-3.1|P-value:9.81E-4||SGD DESC:Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase Gene:PDR12(YPL058C)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity Gene:PDR8(YLR266C)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PHO4(YFR034C)|FD-Score:5.08|P-value:1.90E-7||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PHO88(YBR106W)|FD-Score:-4.39|P-value:5.60E-6||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PKP2(YGL059W)|FD-Score:-3.29|P-value:4.98E-4||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:RFU1(YLR073C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RNH203(YLR154C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome Gene:ROM2(YLR371W)|FD-Score:-4.28|P-value:9.31E-6||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL27B(YDR471W)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RTT106(YNL206C)|FD-Score:-3.13|P-value:8.82E-4||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:SAC1(YKL212W)|FD-Score:-4.05|P-value:2.51E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SBP1(YHL034C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 Gene:SEC72(YLR292C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SEY1(YOR165W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SHU1(YHL006C)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SPR1(YOR190W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:SPT2(YER161C)|FD-Score:-4.52|P-value:3.13E-6||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SQS1(YNL224C)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:SWI4(YER111C)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:TAN1(YGL232W)|FD-Score:4.83|P-value:6.95E-7||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TAT1(YBR069C)|FD-Score:5.69|P-value:6.51E-9||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDH1(YJL052W)|FD-Score:4.1|P-value:2.02E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TFB6(YOR352W)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TRP1(YDR007W)|FD-Score:6.83|P-value:4.23E-12||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:6.79|P-value:5.70E-12||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:10.4|P-value:1.02E-25||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:8.64|P-value:2.89E-18||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:7.57|P-value:1.93E-14||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TUM1(YOR251C)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:TVP38(YKR088C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBP12(YJL197W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:UBP7(YIL156W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions Gene:VPH1(YOR270C)|FD-Score:-4.13|P-value:1.79E-5||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS3(YDR495C)|FD-Score:-4.09|P-value:2.11E-5||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS74(YDR372C)|FD-Score:-5.15|P-value:1.30E-7||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YCR025C(YCR025C_d)|FD-Score:5.24|P-value:7.99E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YDL183C(YDL183C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR008C(YDR008C_d)|FD-Score:8.2|P-value:1.22E-16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFL051C(YFL051C_p)|FD-Score:-3.12|P-value:8.99E-4||SGD DESC:Putative protein of unknown function; YFL051C is not an essential gene Gene:YGL006W-A(YGL006W-A_p)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YIH1(YCR059C)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YJR003C(YJR003C_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis Gene:YML094C-A(YML094C-A_d)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YML122C(YML122C_d)|FD-Score:-3.1|P-value:9.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR315W(YMR315W)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YNL122C(YNL122C_p)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YNL146W(YNL146W_p)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene Gene:YOL098C(YOL098C_p)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Putative metalloprotease Gene:YPK1(YKL126W)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL114W(YPL114W_d)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index Gene:YPR114W(YPR114W_p)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Putative protein of unknown function Gene:YPT6(YLR262C)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:AIF1(YNR074C)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase Gene:AIM22(YJL046W)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss Gene:ANS1(YHR126C_p)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Putative GPI protein; transcription dependent upon Azf1p Gene:ARO1(YDR127W)|FD-Score:15.3|P-value:2.10E-53||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARP5(YNL059C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ASH1(YKL185W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate Gene:ASM4(YDL088C)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:AZR1(YGR224W)|FD-Score:6.25|P-value:2.10E-10||SGD DESC:Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole Gene:BCH1(YMR237W)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BDF1(YLR399C)|FD-Score:6.06|P-value:6.96E-10||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BDH1(YAL060W)|FD-Score:4|P-value:3.13E-5||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:BIO3(YNR058W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CCP1(YKR066C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CMC4(YMR194C-B)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs Gene:CRH1(YGR189C)|FD-Score:-4.02|P-value:2.97E-5||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:CYS3(YAL012W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:CYS4(YGR155W)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAL81(YIR023W)|FD-Score:6.48|P-value:4.45E-11||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCI1(YOR180C)|FD-Score:4.31|P-value:8.26E-6||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DFG5(YMR238W)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DGA1(YOR245C)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles Gene:DSF2(YBR007C_p)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DUG1(YFR044C)|FD-Score:4.36|P-value:6.45E-6||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:DUS4(YLR405W)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:ERG4(YGL012W)|FD-Score:-3.22|P-value:6.43E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FUB1(YCR076C)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene Gene:GCN4(YEL009C)|FD-Score:6.65|P-value:1.51E-11||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCV1(YDR019C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm Gene:GPR1(YDL035C)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HAP5(YOR358W)|FD-Score:3.74|P-value:9.34E-5||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex Gene:HNT3(YOR258W)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:JEM1(YJL073W)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KAP114(YGL241W)|FD-Score:4.28|P-value:9.47E-6||SGD DESC:Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress Gene:KEL2(YGR238C)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LHS1(YKL073W)|FD-Score:-3.32|P-value:4.45E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MRP2(YPR166C)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL44(YMR225C)|FD-Score:-3.22|P-value:6.40E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MTC3(YGL226W)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:NBA1(YOL070C)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:NHP6A(YPR052C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:OPI3(YJR073C)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PAU11(YGL261C_p)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PAU24(YBR301W)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PCS60(YBR222C)|FD-Score:-3.1|P-value:9.81E-4||SGD DESC:Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase Gene:PDR12(YPL058C)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity Gene:PDR8(YLR266C)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PHO4(YFR034C)|FD-Score:5.08|P-value:1.90E-7||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PHO88(YBR106W)|FD-Score:-4.39|P-value:5.60E-6||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PKP2(YGL059W)|FD-Score:-3.29|P-value:4.98E-4||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:RFU1(YLR073C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RNH203(YLR154C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome Gene:ROM2(YLR371W)|FD-Score:-4.28|P-value:9.31E-6||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL27B(YDR471W)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RTT106(YNL206C)|FD-Score:-3.13|P-value:8.82E-4||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:SAC1(YKL212W)|FD-Score:-4.05|P-value:2.51E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SBP1(YHL034C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 Gene:SEC72(YLR292C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SEY1(YOR165W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SHU1(YHL006C)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SPR1(YOR190W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:SPT2(YER161C)|FD-Score:-4.52|P-value:3.13E-6||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SQS1(YNL224C)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:SWI4(YER111C)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:TAN1(YGL232W)|FD-Score:4.83|P-value:6.95E-7||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TAT1(YBR069C)|FD-Score:5.69|P-value:6.51E-9||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDH1(YJL052W)|FD-Score:4.1|P-value:2.02E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TFB6(YOR352W)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TRP1(YDR007W)|FD-Score:6.83|P-value:4.23E-12||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:6.79|P-value:5.70E-12||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:10.4|P-value:1.02E-25||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:8.64|P-value:2.89E-18||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:7.57|P-value:1.93E-14||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TUM1(YOR251C)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:TVP38(YKR088C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBP12(YJL197W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:UBP7(YIL156W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions Gene:VPH1(YOR270C)|FD-Score:-4.13|P-value:1.79E-5||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS3(YDR495C)|FD-Score:-4.09|P-value:2.11E-5||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS74(YDR372C)|FD-Score:-5.15|P-value:1.30E-7||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YCR025C(YCR025C_d)|FD-Score:5.24|P-value:7.99E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YDL183C(YDL183C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR008C(YDR008C_d)|FD-Score:8.2|P-value:1.22E-16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFL051C(YFL051C_p)|FD-Score:-3.12|P-value:8.99E-4||SGD DESC:Putative protein of unknown function; YFL051C is not an essential gene Gene:YGL006W-A(YGL006W-A_p)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YIH1(YCR059C)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YJR003C(YJR003C_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis Gene:YML094C-A(YML094C-A_d)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YML122C(YML122C_d)|FD-Score:-3.1|P-value:9.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR315W(YMR315W)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YNL122C(YNL122C_p)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YNL146W(YNL146W_p)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene Gene:YOL098C(YOL098C_p)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Putative metalloprotease Gene:YPK1(YKL126W)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL114W(YPL114W_d)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index Gene:YPR114W(YPR114W_p)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Putative protein of unknown function Gene:YPT6(YLR262C)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR115C_d5.715.59E-90.72YGR115C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
YML049C5.052.19E-70.72RSE1Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport
YOR250C4.846.34E-70.72CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YHR019C4.523.03E-60.72DED81Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA
YMR033W3.807.13E-50.02ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YOR262W3.797.64E-50.09GPN2Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion
YGL011C3.701.07E-40.03SCL1Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
YHR072W3.671.21E-40.14ERG7Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
YGR251W3.532.11E-40.03NOP19Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes
YDR201W3.502.32E-40.04SPC19Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
YNL137C3.472.65E-40.02NAM9Mitochondrial ribosomal component of the small subunit
YML010W3.452.84E-40.01SPT5Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair
YAL003W3.442.96E-40.05EFB1Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site
YJL034W3.393.55E-40.18KAR2ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
YCL031C3.216.69E-40.01RRP7Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR127W15.302.10E-53ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YKL211C10.401.02E-25TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR354W8.642.89E-18TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YDR008C_d8.201.22E-16YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YGL026C7.571.93E-14TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YDR007W6.834.23E-12TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YER090W6.795.70E-12TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YEL009C6.651.51E-11GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YIR023W6.484.45E-11DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YGR224W6.252.10E-10AZR1Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole
YLR399C6.066.96E-10BDF1Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p
YBR069C5.696.51E-9TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YCR025C_d5.247.99E-8YCR025C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene
YOR258W5.191.06E-7HNT3DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress
YFR034C5.081.90E-7PHO4Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability

GO enrichment analysis for SGTC_474
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4365.34E-272SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.025641
0.3961.64E-220SGTC_18825615643 20.0 μMMiscellaneous22530750.028169TRP & mitochondrial translation
0.3932.49E-217SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0338983
0.3921.17E-216SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.0175439
0.3753.03E-196SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0857143
0.3741.35E-195SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0338983
0.3744.51E-195SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous1273210NATRP & mitochondrial translation
0.3564.12E-176SGTC_1636st007707 68.9 μMTimTec (Natural product derivative library)4030510.015873
0.3478.10E-167SGTC_1699st031318 29.1 μMTimTec (Natural product derivative library)53406580.0350877
0.3472.41E-166SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)30151890.263158
0.3452.94E-164SGTC_12191469-0211 33.1 μMChemDiv (Drug-like library)68222820.0298507
0.3433.22E-162SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0338983
0.3422.54E-161SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0895522
0.3397.70E-159SGTC_18815652484 16.0 μMMiscellaneous22551290.0285714TRP & mitochondrial translation
0.3372.28E-156SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.25

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_438sphingosine6.67 μM0.55280335ICCB bioactive library299.491884.82433
SGTC_436n,n-dimethylsphingosine3.3 μM0.3255282309ICCB bioactive library327.545045.79223
SGTC_1616st00273456.1 μM0.3243244026101TimTec (Natural product derivative library)356.53997.40224
SGTC_2582agaric acid100 μM0.28571412629Microsource (Natural product library)416.54885.98647redox potentiating
SGTC_1573oleylamine1.06 μM0.2727275356789TimTec (Pure natural product library)267.493086.62311
SGTC_1876myriocin605 nM0.269231301119Miscellaneous401.537460.28457
SGTC_2524avocatin b10.57 μM0.2631586710748Microsource (Natural product library)570.884248.94166
SGTC_2618avocadyne43.43 μM0.2631583015189Microsource (Natural product library)284.434184.75833
SGTC_3061glucosamine200 μM0.25714391431Miscellaneous215.63206-2.69966
SGTC_1617st00290678 μM0.255150859TimTec (Natural product derivative library)256.381024.51413