α-linolenic acid

(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid

Alpha-linolenic acid (ALA) is a polyunsaturated n−3 (omega-3) fatty acid. ALA has been noted to be a reactive oxygen species scavenger that temporarily improves cell proliferation.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_475
Screen concentration 10.0 μM
Source ICCB bioactive library
PubChem CID 5280934
SMILES CCC=CCC=CCC=CCCCCCCCC(=O)O
Standardized SMILES CCC=CCC=CCC=CCCCCCCCC(=O)O
Molecular weight 278.4296
ALogP 5.97
H-bond donor count 1
H-bond acceptor count 2
Response signature excess fatty acid

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.22
% growth inhibition (Hom. pool) 5.47


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5280934
Download HIP data (tab-delimited text)  (excel)
Gene:CDC25(YLR310C)|FD-Score:-4.4|P-value:5.30E-6|Clearance:0||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:DRE2(YKR071C)|FD-Score:3.72|P-value:1.00E-4|Clearance:0.02||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:FAS1(YKL182W)|FD-Score:3.16|P-value:7.87E-4|Clearance:0.02||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:IMP3(YHR148W)|FD-Score:3.11|P-value:9.35E-4|Clearance:0.09||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:MDN1(YLR106C)|FD-Score:4.57|P-value:2.45E-6|Clearance:0.63||SGD DESC:Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus Gene:MED4(YOR174W)|FD-Score:5.44|P-value:2.61E-8|Clearance:0.63||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NAR1(YNL240C)|FD-Score:-3.25|P-value:5.79E-4|Clearance:0||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:PRP45(YAL032C)|FD-Score:3.22|P-value:6.51E-4|Clearance:0.06||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:RAM2(YKL019W)|FD-Score:-3.41|P-value:3.25E-4|Clearance:0||SGD DESC:Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor Gene:RPA190(YOR341W)|FD-Score:3.28|P-value:5.13E-4|Clearance:0.07||SGD DESC:RNA polymerase I largest subunit A190 Gene:RPN11(YFR004W)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.13||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPT5(YOR117W)|FD-Score:4.85|P-value:6.29E-7|Clearance:0.63||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RSC58(YLR033W)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.29||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:3.93|P-value:4.16E-5|Clearance:0.22||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:5.05|P-value:2.24E-7|Clearance:0.63||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:5.72|P-value:5.37E-9|Clearance:0.63||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SGV1(YPR161C)|FD-Score:-3.7|P-value:1.06E-4|Clearance:0||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SRB6(YBR253W)|FD-Score:3.12|P-value:9.11E-4|Clearance:0.01||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:TIF35(YDR429C)|FD-Score:-3.78|P-value:7.97E-5|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TOM40(YMR203W)|FD-Score:3.14|P-value:8.44E-4|Clearance:0.02||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TUB4(YLR212C)|FD-Score:-3.47|P-value:2.60E-4|Clearance:0||SGD DESC:Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress Gene:YJL195C(YJL195C_d)|FD-Score:-3.21|P-value:6.57E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:CDC25(YLR310C)|FD-Score:-4.4|P-value:5.30E-6|Clearance:0||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:DRE2(YKR071C)|FD-Score:3.72|P-value:1.00E-4|Clearance:0.02||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:FAS1(YKL182W)|FD-Score:3.16|P-value:7.87E-4|Clearance:0.02||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:IMP3(YHR148W)|FD-Score:3.11|P-value:9.35E-4|Clearance:0.09||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:MDN1(YLR106C)|FD-Score:4.57|P-value:2.45E-6|Clearance:0.63||SGD DESC:Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus Gene:MED4(YOR174W)|FD-Score:5.44|P-value:2.61E-8|Clearance:0.63||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NAR1(YNL240C)|FD-Score:-3.25|P-value:5.79E-4|Clearance:0||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:PRP45(YAL032C)|FD-Score:3.22|P-value:6.51E-4|Clearance:0.06||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:RAM2(YKL019W)|FD-Score:-3.41|P-value:3.25E-4|Clearance:0||SGD DESC:Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor Gene:RPA190(YOR341W)|FD-Score:3.28|P-value:5.13E-4|Clearance:0.07||SGD DESC:RNA polymerase I largest subunit A190 Gene:RPN11(YFR004W)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.13||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPT5(YOR117W)|FD-Score:4.85|P-value:6.29E-7|Clearance:0.63||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RSC58(YLR033W)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.29||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:3.93|P-value:4.16E-5|Clearance:0.22||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:5.05|P-value:2.24E-7|Clearance:0.63||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:5.72|P-value:5.37E-9|Clearance:0.63||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SGV1(YPR161C)|FD-Score:-3.7|P-value:1.06E-4|Clearance:0||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SRB6(YBR253W)|FD-Score:3.12|P-value:9.11E-4|Clearance:0.01||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:TIF35(YDR429C)|FD-Score:-3.78|P-value:7.97E-5|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TOM40(YMR203W)|FD-Score:3.14|P-value:8.44E-4|Clearance:0.02||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TUB4(YLR212C)|FD-Score:-3.47|P-value:2.60E-4|Clearance:0||SGD DESC:Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress Gene:YJL195C(YJL195C_d)|FD-Score:-3.21|P-value:6.57E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5280934
Download HOP data (tab-delimited text)  (excel)
Gene:AAP1(YHR047C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ACE2(YLR131C)|FD-Score:4.53|P-value:2.89E-6||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AFT2(YPL202C)|FD-Score:4.89|P-value:4.99E-7||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:AIM11(YER093C-A)|FD-Score:-3.39|P-value:3.51E-4||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:ARO10(YDR380W)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Phenylpyruvate decarboxylase, catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway Gene:ATP22(YDR350C)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:Specific translational activator for the mitochondrial ATP6 mRNA, encoding a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane Gene:BAG7(YOR134W)|FD-Score:4.99|P-value:3.02E-7||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:BDF1(YLR399C)|FD-Score:-3.84|P-value:6.21E-5||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BST1(YFL025C)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:COG6(YNL041C)|FD-Score:7.07|P-value:7.50E-13||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:7.14|P-value:4.61E-13||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:8.01|P-value:5.66E-16||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COS1(YNL336W)|FD-Score:5.89|P-value:1.96E-9||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CPR7(YJR032W)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CRG1(YHR209W)|FD-Score:-3.71|P-value:1.06E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:DNF3(YMR162C)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase Gene:ECM31(YBR176W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate Gene:EDC1(YGL222C)|FD-Score:-4.35|P-value:6.75E-6||SGD DESC:RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress Gene:ENT3(YJR125C)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p Gene:FYV6(YNL133C)|FD-Score:-5.1|P-value:1.66E-7||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:HHF1(YBR009C)|FD-Score:-4.41|P-value:5.25E-6||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HIR2(YOR038C)|FD-Score:3.78|P-value:7.69E-5||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p Gene:KCS1(YDR017C)|FD-Score:4.59|P-value:2.17E-6||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:LOA1(YPR139C)|FD-Score:-3.17|P-value:7.63E-4||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:LRO1(YNR008W)|FD-Score:29.3|P-value:7.98E-189||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:MDM34(YGL219C)|FD-Score:-4.7|P-value:1.33E-6||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MSB3(YNL293W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:NMA111(YNL123W)|FD-Score:-4.2|P-value:1.33E-5||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NTH2(YBR001C)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PHA2(YNL316C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PRM9(YAR031W)|FD-Score:4.61|P-value:2.01E-6||SGD DESC:Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family Gene:PTK2(YJR059W)|FD-Score:4.87|P-value:5.46E-7||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PTP3(YER075C)|FD-Score:4.45|P-value:4.30E-6||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm Gene:QCR6(YFR033C)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space Gene:REF2(YDR195W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:ROM2(YLR371W)|FD-Score:4.63|P-value:1.79E-6||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL16A(YIL133C)|FD-Score:3.85|P-value:5.99E-5||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SAC1(YKL212W)|FD-Score:-3.13|P-value:8.60E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SFP1(YLR403W)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SGS1(YMR190C)|FD-Score:3.84|P-value:6.25E-5||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SIS2(YKR072C)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SSE1(YPL106C)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:TDA8(YAL064C-A_p)|FD-Score:3.92|P-value:4.49E-5||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TGL2(YDR058C)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TIR4(YOR009W)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock Gene:UBC13(YDR092W)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus Gene:VTS1(YOR359W)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity Gene:WTM2(YOR229W)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats Gene:YCR090C(YCR090C_p)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YDL062W(YDL062W_d)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential Gene:YDR230W(YDR230W_d)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20 Gene:YDR290W(YDR290W_d)|FD-Score:-3.39|P-value:3.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YHL005C(YHL005C_d)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W Gene:YHL015W-A(YHL015W-A_p)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:Putative protein of unknown function Gene:YIL014C-A(YIL014C-A_p)|FD-Score:-4.6|P-value:2.08E-6||SGD DESC:Putative protein of unknown function Gene:YIP4(YGL198W)|FD-Score:-3.11|P-value:9.29E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YKL053W(YKL053W_d)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Gene:YLR171W(YLR171W_d)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR446W(YLR446W_p)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene Gene:YML002W(YML002W_p)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YMR210W(YMR210W)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification Gene:YNL010W(YNL010W_p)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNL155W(YNL155W_p)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Protein of unknown function; contains a DHHC domain and an AN1-type zinc finger; predicted to have thiol-disulfide oxidoreductase active site; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YOL046C(YOL046C_d)|FD-Score:4.51|P-value:3.26E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W Gene:YPR126C(YPR126C_d)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT53(YNL093W)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:YSC83(YHR017W)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:AAP1(YHR047C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ACE2(YLR131C)|FD-Score:4.53|P-value:2.89E-6||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AFT2(YPL202C)|FD-Score:4.89|P-value:4.99E-7||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:AIM11(YER093C-A)|FD-Score:-3.39|P-value:3.51E-4||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:ARO10(YDR380W)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Phenylpyruvate decarboxylase, catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway Gene:ATP22(YDR350C)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:Specific translational activator for the mitochondrial ATP6 mRNA, encoding a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane Gene:BAG7(YOR134W)|FD-Score:4.99|P-value:3.02E-7||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:BDF1(YLR399C)|FD-Score:-3.84|P-value:6.21E-5||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BST1(YFL025C)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:COG6(YNL041C)|FD-Score:7.07|P-value:7.50E-13||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:7.14|P-value:4.61E-13||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:8.01|P-value:5.66E-16||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COS1(YNL336W)|FD-Score:5.89|P-value:1.96E-9||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CPR7(YJR032W)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CRG1(YHR209W)|FD-Score:-3.71|P-value:1.06E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:DNF3(YMR162C)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase Gene:ECM31(YBR176W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate Gene:EDC1(YGL222C)|FD-Score:-4.35|P-value:6.75E-6||SGD DESC:RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress Gene:ENT3(YJR125C)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p Gene:FYV6(YNL133C)|FD-Score:-5.1|P-value:1.66E-7||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:HHF1(YBR009C)|FD-Score:-4.41|P-value:5.25E-6||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HIR2(YOR038C)|FD-Score:3.78|P-value:7.69E-5||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p Gene:KCS1(YDR017C)|FD-Score:4.59|P-value:2.17E-6||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:LOA1(YPR139C)|FD-Score:-3.17|P-value:7.63E-4||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:LRO1(YNR008W)|FD-Score:29.3|P-value:7.98E-189||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:MDM34(YGL219C)|FD-Score:-4.7|P-value:1.33E-6||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MSB3(YNL293W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:NMA111(YNL123W)|FD-Score:-4.2|P-value:1.33E-5||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NTH2(YBR001C)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PHA2(YNL316C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PRM9(YAR031W)|FD-Score:4.61|P-value:2.01E-6||SGD DESC:Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family Gene:PTK2(YJR059W)|FD-Score:4.87|P-value:5.46E-7||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PTP3(YER075C)|FD-Score:4.45|P-value:4.30E-6||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm Gene:QCR6(YFR033C)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space Gene:REF2(YDR195W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:ROM2(YLR371W)|FD-Score:4.63|P-value:1.79E-6||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL16A(YIL133C)|FD-Score:3.85|P-value:5.99E-5||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SAC1(YKL212W)|FD-Score:-3.13|P-value:8.60E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SFP1(YLR403W)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SGS1(YMR190C)|FD-Score:3.84|P-value:6.25E-5||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SIS2(YKR072C)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SSE1(YPL106C)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:TDA8(YAL064C-A_p)|FD-Score:3.92|P-value:4.49E-5||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TGL2(YDR058C)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TIR4(YOR009W)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock Gene:UBC13(YDR092W)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus Gene:VTS1(YOR359W)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity Gene:WTM2(YOR229W)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats Gene:YCR090C(YCR090C_p)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YDL062W(YDL062W_d)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential Gene:YDR230W(YDR230W_d)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20 Gene:YDR290W(YDR290W_d)|FD-Score:-3.39|P-value:3.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YHL005C(YHL005C_d)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W Gene:YHL015W-A(YHL015W-A_p)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:Putative protein of unknown function Gene:YIL014C-A(YIL014C-A_p)|FD-Score:-4.6|P-value:2.08E-6||SGD DESC:Putative protein of unknown function Gene:YIP4(YGL198W)|FD-Score:-3.11|P-value:9.29E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YKL053W(YKL053W_d)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Gene:YLR171W(YLR171W_d)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR446W(YLR446W_p)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene Gene:YML002W(YML002W_p)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YMR210W(YMR210W)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification Gene:YNL010W(YNL010W_p)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNL155W(YNL155W_p)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Protein of unknown function; contains a DHHC domain and an AN1-type zinc finger; predicted to have thiol-disulfide oxidoreductase active site; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YOL046C(YOL046C_d)|FD-Score:4.51|P-value:3.26E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W Gene:YPR126C(YPR126C_d)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT53(YNL093W)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:YSC83(YHR017W)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YML127W5.725.37E-90.63RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YOR174W5.442.61E-80.63MED4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YFR037C5.052.24E-70.63RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YOR117W4.856.29E-70.63RPT5One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
YLR106C4.572.45E-60.63MDN1Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus
YCR052W3.934.16E-50.22RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YKR071C3.721.00E-40.02DRE2Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress
YFR004W3.701.09E-40.13RPN11Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
YLR033W3.571.79E-40.29RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YOR341W3.285.13E-40.07RPA190RNA polymerase I largest subunit A190
YAL032C3.226.51E-40.06PRP45Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
YKL182W3.167.87E-40.02FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YMR203W3.148.44E-40.02TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YBR253W3.129.11E-40.01SRB6Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YHR148W3.119.35E-40.09IMP3Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNR008W29.307.98E-189LRO1Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase
YML071C8.015.66E-16COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YGL005C7.144.61E-13COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YNL041C7.077.50E-13COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YNL336W5.891.96E-9COS1Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
YLR171W_d5.151.31E-7YLR171W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOR134W4.993.02E-7BAG7Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p
YPL202C4.894.99E-7AFT2Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication
YJR059W4.875.46E-7PTK2Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake
YLR371W4.631.79E-6ROM2GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p
YAR031W4.612.01E-6PRM9Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family
YDR017C4.592.17E-6KCS1Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance
YLR131C4.532.89E-6ACE2Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
YOL046C_d4.513.26E-6YOL046C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W
YER075C4.454.30E-6PTP3Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm

GO enrichment analysis for SGTC_475
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4730SGTC_450kpi-0036 14.9 μMChemDiv (Drug-like library)27950210.0377358excess fatty acid
0.4091.49E-236SGTC_15110906-3109 28.5 μMChemDiv (Drug-like library)28821800.0153846excess fatty acid
0.3583.77E-178SGTC_410α-linolenic acid 100.0 μMMiscellaneous8601excess fatty acid
0.2718.52E-100SGTC_6281636-0253 16.0 μMChemDiv (Drug-like library)4456143NAsphingolipid biosynthesis & PDR1
0.2566.04E-89SGTC_350k064-0020 19.8 μMChemDiv (Drug-like library)2790705NAheme requiring
0.2541.09E-87SGTC_7431275-0778 158.0 μMChemDiv (Drug-like library)28492570.0350877excess fatty acid
0.2034.87E-56SGTC_1150527-0192 112.0 μMChemDiv (Drug-like library)42339310.016129
0.2015.71E-55SGTC_1434032-1194 15.7 μMChemDiv (Drug-like library)18069630.0645161
0.1904.66E-49SGTC_9221866-0035 480.0 μMChemDiv (Drug-like library)31561690.0149254excess fatty acid
0.1884.23E-48SGTC_8520418-0143 439.0 μMChemDiv (Drug-like library)53692290.0377358calcium & mitochondrial duress
0.1701.01E-39SGTC_2766oltipraz 83.9 μMMiscellaneous473180.0185185heme requiring
0.1582.44E-34SGTC_643k048-0106 28.4 μMChemDiv (Drug-like library)67639200.046875RSC & ERG11
0.1553.83E-33SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.0273973Golgi
0.1526.69E-32SGTC_960929-0093 37.3 μMChemDiv (Drug-like library)69915790.030303calcium & mitochondrial duress
0.1503.29E-31SGTC_348k064-0012 10.7 μMChemDiv (Drug-like library)5066323NA60S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_410α-linolenic acid100 μM1860Miscellaneous278.42965.97112excess fatty acid
SGTC_476mead acid10 μM0.7419355312531ICCB bioactive library306.482766.88312
SGTC_409arachidonic acid100 μM0.709677231Miscellaneous304.466886.43912
SGTC_479n-linoleoylglycine10 μM0.5641036433346ICCB bioactive library337.49685.47623
SGTC_478N-arachidonylglycine10 μM0.55283389ICCB bioactive library361.51825.49923
SGTC_477mead ethanolamide10 μM0.45238116061185ICCB bioactive library349.550565.92222
SGTC_1617st00290678 μM0.4411765150859TimTec (Natural product derivative library)256.381024.51413
SGTC_4732-arachidonoylglycerol10 μM0.4186055282280ICCB bioactive library378.545425.61424
SGTC_1616st00273456.1 μM0.3947374026101TimTec (Natural product derivative library)356.53997.40224
SGTC_1876myriocin605 nM0.346154301119Miscellaneous401.537460.28457