mead ethanolamide

(5Z,8Z,11Z)-N-(2-hydroxyethyl)icosa-5,8,11-trienamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_477
Screen concentration 10.0 μM
Source ICCB bioactive library
PubChem CID 16061185
SMILES CCCCCCCCC=CCC=CCC=CCCCC(=O)NCCO
Standardized SMILES CCCCCCCCC=CCC=CCC=CCCCC(=O)NCCO
Molecular weight 349.5506
ALogP 5.92
H-bond donor count 2
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) 2.87


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 16061185
Download HIP data (tab-delimited text)  (excel)

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 16061185
Download HOP data (tab-delimited text)  (excel)
Gene:ATG32(YIL146C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane Gene:BOP3(YNL042W)|FD-Score:3.74|P-value:9.13E-5||SGD DESC:Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury Gene:CSF1(YLR087C)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ERF2(YLR246W)|FD-Score:5.14|P-value:1.40E-7||SGD DESC:Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole Gene:ERG3(YLR056W)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:KCS1(YDR017C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:LNP1(YHR192W_p)|FD-Score:-4.85|P-value:6.29E-7||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:LTP1(YPR073C)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine Gene:NUP53(YMR153W)|FD-Score:4.42|P-value:5.01E-6||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:PKH3(YDR466W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:RFU1(YLR073C)|FD-Score:-3.19|P-value:7.19E-4||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RPL16A(YIL133C)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SAP1(YER047C)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:TLG2(YOL018C)|FD-Score:-3.92|P-value:4.44E-5||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP4(YDR354W)|FD-Score:-3.8|P-value:7.22E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:YLR456W(YLR456W_p)|FD-Score:4.4|P-value:5.33E-6||SGD DESC:Putative pyridoxal 5'-phosphate synthase; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication Gene:YML119W(YML119W_p)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate Gene:ATG32(YIL146C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane Gene:BOP3(YNL042W)|FD-Score:3.74|P-value:9.13E-5||SGD DESC:Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury Gene:CSF1(YLR087C)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ERF2(YLR246W)|FD-Score:5.14|P-value:1.40E-7||SGD DESC:Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole Gene:ERG3(YLR056W)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:KCS1(YDR017C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:LNP1(YHR192W_p)|FD-Score:-4.85|P-value:6.29E-7||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:LTP1(YPR073C)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine Gene:NUP53(YMR153W)|FD-Score:4.42|P-value:5.01E-6||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:PKH3(YDR466W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:RFU1(YLR073C)|FD-Score:-3.19|P-value:7.19E-4||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RPL16A(YIL133C)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SAP1(YER047C)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:TLG2(YOL018C)|FD-Score:-3.92|P-value:4.44E-5||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP4(YDR354W)|FD-Score:-3.8|P-value:7.22E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:YLR456W(YLR456W_p)|FD-Score:4.4|P-value:5.33E-6||SGD DESC:Putative pyridoxal 5'-phosphate synthase; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication Gene:YML119W(YML119W_p)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOL149W2.300.010700.10DCP1Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress
YDR339C2.230.012800.10FCF1Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p
YEL058W2.130.016400.05PCM1Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors
YLR305C2.080.018700.00STT4Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization
YLL036C2.080.018800.03PRP19Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain
YOL127W2.050.020100.06RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
YGR113W1.990.023400.02DAM1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments
YDL205C1.970.024700.07HEM3Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
YLR127C1.900.028800.01APC2Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p
YBR190W_d1.890.029307.60E-4YBR190W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
YNR011C1.890.029400.01PRP2RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16
YOL139C1.870.030400.02CDC33mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth
YPL007C1.860.031804.04E-4TFC8One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90
YBL035C1.850.031800.02POL12B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation
YPL190C1.830.033600.00NAB3RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR246W5.141.40E-7ERF2Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole
YMR153W4.425.01E-6NUP53FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication
YLR456W_p4.405.33E-6YLR456W_pPutative pyridoxal 5'-phosphate synthase; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication
YIL146C4.201.31E-5ATG32Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane
YNL042W3.749.13E-5BOP3Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury
YDR017C3.462.72E-4KCS1Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance
YLR087C3.373.75E-4CSF1Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YML119W_p3.275.46E-4YML119W_pPutative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate
YPR073C3.216.57E-4LTP1Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine
YER047C3.158.19E-4SAP1Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system
YBR108W3.030.00122AIM3Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss
YMR158W3.020.00127MRPS8Mitochondrial ribosomal protein of the small subunit
YMR030W2.870.00205RSF1Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes
YKL026C2.710.00335GPX1Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress
YOR038C2.700.00351HIR2Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p

GO enrichment analysis for SGTC_477
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0831.91E-10SGTC_9373122-0005 46.7 μMChemDiv (Drug-like library)7595350.0298507
0.0791.22E-9SGTC_411enrofloxacin 100.0 μMMiscellaneous711880.0921053DNA damage response
0.0791.37E-9SGTC_33409146634 11.1 μMChembridge (Drug-like library)49073870.0897436
0.0722.87E-8SGTC_29619083290 32.5 μMChembridge (Drug-like library)177414900.0615385
0.0681.45E-7SGTC_7820083-0095 30.5 μMChemDiv (Drug-like library)35917290.05
0.0656.28E-7SGTC_492tmb-8 126.0 μMICCB bioactive library54940.125
0.0631.07E-6SGTC_32239129970 49.5 μMChembridge (Drug-like library)172483860.0704225
0.0613.03E-6SGTC_32609137109 49.5 μMChembridge (Drug-like library)174783460.13559360S ribosome export
0.0561.86E-5SGTC_8810833-0569 170.0 μMChemDiv (Drug-like library)54187000.0724638
0.0552.63E-5SGTC_31369095672 49.5 μMChembridge (Drug-like library)172173800.0519481
0.0552.77E-5SGTC_22167189485 200.0 μMChembridge (Fragment library)7312730.078125
0.0535.34E-5SGTC_30529092717 49.5 μMChembridge (Drug-like library)252375340.153846
0.0525.67E-5SGTC_917harmaline 728.1 nMTimTec (Natural product library)52809510.0144928ergosterol biosynthesis
0.0526.25E-5SGTC_32279130768 49.5 μMChembridge (Drug-like library)177208990.121622
0.0482.22E-4SGTC_30539095903 49.5 μMChembridge (Drug-like library)171740180.101449Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_478N-arachidonylglycine10 μM0.6755283389ICCB bioactive library361.51825.49923
SGTC_479n-linoleoylglycine10 μM0.6585376433346ICCB bioactive library337.49685.47623
SGTC_476mead acid10 μM0.6052635312531ICCB bioactive library306.482766.88312
SGTC_409arachidonic acid100 μM0.578947231Miscellaneous304.466886.43912
SGTC_4732-arachidonoylglycerol10 μM0.5111115282280ICCB bioactive library378.545425.61424
SGTC_410α-linolenic acid100 μM0.452381860Miscellaneous278.42965.97112excess fatty acid
SGTC_475α-linolenic acid10 μM0.4523815280934ICCB bioactive library278.42965.97112excess fatty acid
SGTC_1573oleylamine1.06 μM0.3589745356789TimTec (Pure natural product library)267.493086.62311
SGTC_1876myriocin605 nM0.350877301119Miscellaneous401.537460.28457
SGTC_438sphingosine6.67 μM0.3191495280335ICCB bioactive library299.491884.82433
SGTC_436n,n-dimethylsphingosine3.3 μM0.3061225282309ICCB bioactive library327.545045.79223