N-arachidonylglycine

2-[[(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoyl]amino]acetic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_478
Screen concentration 10.0 μM
Source ICCB bioactive library
PubChem CID 5283389
SMILES CCCCCC=CCC=CCC=CCC=CCCCC(=O)NCC(=O)O
Standardized SMILES CCCCCC=CCC=CCC=CCC=CCCCC(=O)NCC(=O)O
Molecular weight 361.5182
ALogP 5.5
H-bond donor count 2
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.3
% growth inhibition (Hom. pool) 4.17


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5283389
Download HIP data (tab-delimited text)  (excel)
Gene:CBF2(YGR140W)|FD-Score:3.45|P-value:2.81E-4|Clearance:0.61||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:YJL015C(YJL015C_d)|FD-Score:-3.1|P-value:9.60E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W Gene:CBF2(YGR140W)|FD-Score:3.45|P-value:2.81E-4|Clearance:0.61||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:YJL015C(YJL015C_d)|FD-Score:-3.1|P-value:9.60E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5283389
Download HOP data (tab-delimited text)  (excel)
Gene:AQR1(YNL065W)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:BDF1(YLR399C)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:CHS7(YHR142W)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:DDI3(YNL335W_p)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:HNT3(YOR258W)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HOP2(YGL033W)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:IRC16(YPR038W_d)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci Gene:KTI11(YBL071W-A)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:PBS2(YJL128C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:SEF1(YBL066C)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Putative transcription factor, has homolog in Kluyveromyces lactis Gene:SLX5(YDL013W)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SRB8(YCR081W)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:YBR226C(YBR226C_d)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YKL075C(YKL075C_p)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YNL024C(YNL024C_p)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Putative methyltransferase; has seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; despite similarity to methyltransferases, null mutant does not display alterations in lysine methylation pattern Gene:AQR1(YNL065W)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:BDF1(YLR399C)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:CHS7(YHR142W)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:DDI3(YNL335W_p)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:HNT3(YOR258W)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HOP2(YGL033W)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:IRC16(YPR038W_d)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci Gene:KTI11(YBL071W-A)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:PBS2(YJL128C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:SEF1(YBL066C)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Putative transcription factor, has homolog in Kluyveromyces lactis Gene:SLX5(YDL013W)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SRB8(YCR081W)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:YBR226C(YBR226C_d)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YKL075C(YKL075C_p)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YNL024C(YNL024C_p)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Putative methyltransferase; has seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; despite similarity to methyltransferases, null mutant does not display alterations in lysine methylation pattern

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR140W3.452.81E-40.61CBF2Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress
YEL019C2.840.002260.12MMS21SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination
YNL113W2.720.003260.07RPC19RNA polymerase subunit AC19, common to RNA polymerases I and III
YNL181W_p2.650.004050.09YNL181W_pPutative oxidoreductase; required for cell viability
YGR083C2.560.005220.04GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YJR064W2.520.005800.22CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YOL149W2.300.010600.07DCP1Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress
YEL058W2.240.012600.17PCM1Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors
YKL193C2.070.019500.00SDS22Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function
YNL178W2.060.019700.03RPS3Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3
YDR246W2.040.020900.06TRS23One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4
YER127W1.980.024108.77E-4LCP5Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus
YFR031C1.970.024200.02SMC2Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus
YLR117C1.960.025100.02CLF1Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
YOL078W1.940.026200.02AVO1Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR142W4.241.13E-5CHS7Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress
YBR226C_d4.211.28E-5YBR226C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W
YNL065W4.121.87E-5AQR1Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
YCR081W4.102.11E-5SRB8Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression
YPR038W_d3.611.54E-4IRC16_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci
YNL335W_p3.403.43E-4DDI3_pProtein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants
YJL128C3.383.57E-4PBS2MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition
YKL075C_p3.324.46E-4YKL075C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin
YBL071W-A3.255.74E-4KTI11Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs
YOR258W3.255.76E-4HNT3DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress
YLR399C3.187.29E-4BDF1Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p
YBL066C3.158.22E-4SEF1Putative transcription factor, has homolog in Kluyveromyces lactis
YDL013W3.158.26E-4SLX5Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers
YOR147W2.960.00154MDM32Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34
YDR148C2.930.00171KGD2Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated

GO enrichment analysis for SGTC_478
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0896.99E-12SGTC_2760etidronate 98.0 μMMiscellaneous33050.0666667
0.0856.67E-11SGTC_30139080133 71.4 μMChembridge (Drug-like library)170501930.0666667
0.0823.56E-10SGTC_1644st010134 20.9 μMTimTec (Natural product derivative library)38181340.0454545mitochondrial processes
0.0813.99E-10SGTC_12720839-0021 27.0 μMChemDiv (Drug-like library)50272500.0333333
0.0791.40E-9SGTC_14894065-0444 182.0 μMChemDiv (Drug-like library)28779680.0133333
0.0781.65E-9SGTC_9123474-0017 2.2 μMChemDiv (Drug-like library)33605500.0724638
0.0705.92E-8SGTC_6653914-0008 307.0 μMChemDiv (Drug-like library)10695490.12
0.0691.22E-7SGTC_12920kpi-0043 232.0 μMChemDiv (Drug-like library)33339050.104478
0.0672.10E-7SGTC_31489097893 49.5 μMChembridge (Drug-like library)171741000.0684932
0.0656.41E-7SGTC_474dl-dihydrosphingosine 5.0 μMICCB bioactive library30587390.166667
0.0649.13E-7SGTC_20305175110 200.0 μMChembridge (Fragment library)28319860.0689655Golgi
0.0649.44E-7SGTC_30359091303 49.5 μMChembridge (Drug-like library)252370020.15
0.0621.57E-6SGTC_29269010726 65.2 μMChembridge (Drug-like library)29942930.09375
0.0621.60E-6SGTC_7593486-0358 89.3 μMChemDiv (Drug-like library)8751030.04
0.0621.67E-6SGTC_1762st045503 25.5 μMTimTec (Natural product derivative library)46455540.0116279

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_479n-linoleoylglycine10 μM0.9142866433346ICCB bioactive library337.49685.47623
SGTC_409arachidonic acid100 μM0.685714231Miscellaneous304.466886.43912
SGTC_477mead ethanolamide10 μM0.67516061185ICCB bioactive library349.550565.92222
SGTC_476mead acid10 μM0.6666675312531ICCB bioactive library306.482766.88312
SGTC_410α-linolenic acid100 μM0.5860Miscellaneous278.42965.97112excess fatty acid
SGTC_475α-linolenic acid10 μM0.55280934ICCB bioactive library278.42965.97112excess fatty acid
SGTC_4732-arachidonoylglycerol10 μM0.4888895282280ICCB bioactive library378.545425.61424
SGTC_1617st00290678 μM0.3414635150859TimTec (Natural product derivative library)256.381024.51413
SGTC_1573oleylamine1.06 μM0.3333335356789TimTec (Pure natural product library)267.493086.62311
SGTC_1876myriocin605 nM0.333333301119Miscellaneous401.537460.28457
SGTC_1616st00273456.1 μM0.3111114026101TimTec (Natural product derivative library)356.53997.40224