fpl-64176

methyl 4-(2-benzylbenzoyl)-2,5-dimethyl-1H-pyrrole-3-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_483
Screen concentration 144.0 μM
Source ICCB bioactive library
PubChem CID 3423
SMILES CC1=C(C(=C(N1)C)C(=O)OC)C(=O)C2=CC=CC=C2CC3=CC=CC=C3
Standardized SMILES COC(=O)c1c(C)[nH]c(C)c1C(=O)c2ccccc2Cc3ccccc3
Molecular weight 347.407
ALogP 4.45
H-bond donor count 1
H-bond acceptor count 3
Response signature cell wall

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.55
% growth inhibition (Hom. pool) 12.93


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3423
Download HIP data (tab-delimited text)  (excel)
Gene:ARP9(YMR033W)|FD-Score:5.36|P-value:4.19E-8|Clearance:0.33||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CYR1(YJL005W)|FD-Score:-3.59|P-value:1.66E-4|Clearance:0||SGD DESC:Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation Gene:FCP1(YMR277W)|FD-Score:4.29|P-value:8.84E-6|Clearance:0.23||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:GLC7(YER133W)|FD-Score:4.07|P-value:2.40E-5|Clearance:0.43||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:PKC1(YBL105C)|FD-Score:3.63|P-value:1.40E-4|Clearance:0.51||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RHO1(YPR165W)|FD-Score:10.8|P-value:2.74E-27|Clearance:4.6||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RSC8(YFR037C)|FD-Score:5.03|P-value:2.51E-7|Clearance:0.61||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC13(YLR208W)|FD-Score:6.06|P-value:6.94E-10|Clearance:0.7||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC14(YMR079W)|FD-Score:3.13|P-value:8.89E-4|Clearance:0.03||SGD DESC:Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs Gene:SEC31(YDL195W)|FD-Score:6.16|P-value:3.74E-10|Clearance:0.1||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:YDL196W(YDL196W_d)|FD-Score:4.42|P-value:5.03E-6|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YTH1(YPR107C)|FD-Score:3.1|P-value:9.78E-4|Clearance:0.04||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ARP9(YMR033W)|FD-Score:5.36|P-value:4.19E-8|Clearance:0.33||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CYR1(YJL005W)|FD-Score:-3.59|P-value:1.66E-4|Clearance:0||SGD DESC:Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation Gene:FCP1(YMR277W)|FD-Score:4.29|P-value:8.84E-6|Clearance:0.23||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:GLC7(YER133W)|FD-Score:4.07|P-value:2.40E-5|Clearance:0.43||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:PKC1(YBL105C)|FD-Score:3.63|P-value:1.40E-4|Clearance:0.51||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RHO1(YPR165W)|FD-Score:10.8|P-value:2.74E-27|Clearance:4.6||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RSC8(YFR037C)|FD-Score:5.03|P-value:2.51E-7|Clearance:0.61||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC13(YLR208W)|FD-Score:6.06|P-value:6.94E-10|Clearance:0.7||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC14(YMR079W)|FD-Score:3.13|P-value:8.89E-4|Clearance:0.03||SGD DESC:Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs Gene:SEC31(YDL195W)|FD-Score:6.16|P-value:3.74E-10|Clearance:0.1||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:YDL196W(YDL196W_d)|FD-Score:4.42|P-value:5.03E-6|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YTH1(YPR107C)|FD-Score:3.1|P-value:9.78E-4|Clearance:0.04||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3423
Download HOP data (tab-delimited text)  (excel)
Gene:ARL1(YBR164C)|FD-Score:-3.73|P-value:9.74E-5||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARO1(YDR127W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO10(YDR380W)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Phenylpyruvate decarboxylase, catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway Gene:ATG32(YIL146C)|FD-Score:-3.21|P-value:6.68E-4||SGD DESC:Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane Gene:BCK1(YJL095W)|FD-Score:6.23|P-value:2.40E-10||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BST1(YFL025C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CLA4(YNL298W)|FD-Score:3.98|P-value:3.45E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG6(YNL041C)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:EMI2(YDR516C)|FD-Score:-3.47|P-value:2.63E-4||SGD DESC:Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:ERG4(YGL012W)|FD-Score:4.35|P-value:6.79E-6||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:GCN2(YDR283C)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GET1(YGL020C)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GPM2(YDL021W)|FD-Score:4.33|P-value:7.59E-6||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:HOP2(YGL033W)|FD-Score:3.82|P-value:6.62E-5||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:IRS4(YKR019C)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MIH1(YMR036C)|FD-Score:3.94|P-value:4.03E-5||SGD DESC:Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 Gene:MSC1(YML128C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:OCA5(YHL029C_p)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:PDR1(YGL013C)|FD-Score:5.14|P-value:1.39E-7||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEP8(YJL053W)|FD-Score:3.74|P-value:9.16E-5||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PTC1(YDL006W)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:RGP1(YDR137W)|FD-Score:3.87|P-value:5.42E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPN4(YDL020C)|FD-Score:4.52|P-value:3.03E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SLT2(YHR030C)|FD-Score:8.14|P-value:2.01E-16||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SPT7(YBR081C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SSD1(YDR293C)|FD-Score:5.42|P-value:2.97E-8||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWP82(YFL049W)|FD-Score:3.88|P-value:5.21E-5||SGD DESC:Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions Gene:TSC3(YBR058C-A)|FD-Score:7.15|P-value:4.44E-13||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URE2(YNL229C)|FD-Score:3.86|P-value:5.62E-5||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VPS51(YKR020W)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:YDL177C(YDL177C_p)|FD-Score:-5.14|P-value:1.34E-7||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YDR220C(YDR220C_d)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:ARL1(YBR164C)|FD-Score:-3.73|P-value:9.74E-5||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARO1(YDR127W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO10(YDR380W)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Phenylpyruvate decarboxylase, catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway Gene:ATG32(YIL146C)|FD-Score:-3.21|P-value:6.68E-4||SGD DESC:Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane Gene:BCK1(YJL095W)|FD-Score:6.23|P-value:2.40E-10||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BST1(YFL025C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CLA4(YNL298W)|FD-Score:3.98|P-value:3.45E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG6(YNL041C)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:EMI2(YDR516C)|FD-Score:-3.47|P-value:2.63E-4||SGD DESC:Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:ERG4(YGL012W)|FD-Score:4.35|P-value:6.79E-6||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:GCN2(YDR283C)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GET1(YGL020C)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GPM2(YDL021W)|FD-Score:4.33|P-value:7.59E-6||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:HOP2(YGL033W)|FD-Score:3.82|P-value:6.62E-5||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:IRS4(YKR019C)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MIH1(YMR036C)|FD-Score:3.94|P-value:4.03E-5||SGD DESC:Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 Gene:MSC1(YML128C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:OCA5(YHL029C_p)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:PDR1(YGL013C)|FD-Score:5.14|P-value:1.39E-7||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEP8(YJL053W)|FD-Score:3.74|P-value:9.16E-5||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PTC1(YDL006W)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:RGP1(YDR137W)|FD-Score:3.87|P-value:5.42E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPN4(YDL020C)|FD-Score:4.52|P-value:3.03E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SLT2(YHR030C)|FD-Score:8.14|P-value:2.01E-16||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SPT7(YBR081C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SSD1(YDR293C)|FD-Score:5.42|P-value:2.97E-8||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWP82(YFL049W)|FD-Score:3.88|P-value:5.21E-5||SGD DESC:Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions Gene:TSC3(YBR058C-A)|FD-Score:7.15|P-value:4.44E-13||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URE2(YNL229C)|FD-Score:3.86|P-value:5.62E-5||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VPS51(YKR020W)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:YDL177C(YDL177C_p)|FD-Score:-5.14|P-value:1.34E-7||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YDR220C(YDR220C_d)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR165W10.802.74E-274.60RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YDL195W6.163.74E-100.10SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YLR208W6.066.94E-100.70SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YMR033W5.364.19E-80.33ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YFR037C5.032.51E-70.61RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YDL196W_d4.425.03E-60.12YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YMR277W4.298.84E-60.23FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
YER133W4.072.40E-50.43GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YBL105C3.631.40E-40.51PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YMR079W3.138.89E-40.03SEC14Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
YPR107C3.109.78E-40.04YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YOR257W3.050.001130.00CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YGL073W3.050.001150.00HSF1Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated
YNR016C3.050.001160.14ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YLR033W2.910.001840.04RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR030C8.142.01E-16SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YBR058C-A7.154.44E-13TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YJL095W6.232.40E-10BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YDR293C5.422.97E-8SSD1Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
YGL013C5.141.39E-7PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YDL020C4.523.03E-6RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YGL012W4.356.79E-6ERG4C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
YDL021W4.337.59E-6GPM2Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event
YDR283C4.171.54E-5GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YNL041C4.082.23E-5COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YNL298W3.983.45E-5CLA4Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p
YMR036C3.944.03E-5MIH1Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
YFL049W3.885.21E-5SWP82Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions
YDR137W3.875.42E-5RGP1Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p
YNL229C3.865.62E-5URE2Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion

GO enrichment analysis for SGTC_483
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3925.61E-216SGTC_8830850-0245 24.6 μMChemDiv (Drug-like library)68232290.0617284cell wall
0.3515.61E-171SGTC_33509150499 3.6 μMChembridge (Drug-like library)49109960.135802cell wall
0.3472.61E-166SGTC_9052-aminofluorene 215.0 μMChemDiv (Drug-like library)15390.0877193cell wall signaling
0.3404.50E-159SGTC_32859112818 20.9 μMChembridge (Drug-like library)170179470.142857cell wall
0.3251.54E-145SGTC_29127964298 10.9 μMChembridge (Drug-like library)29758500.166667cell wall
0.3123.12E-133SGTC_22727805186 180.0 μMChembridge (Fragment library)29560620.102941cell wall signaling
0.3073.73E-129SGTC_1874staurosporine 7.6 μMICCB bioactive library468641930.0978261cell wall signaling
0.2972.71E-120SGTC_33319141060 2.0 μMChembridge (Drug-like library)49065670.1cell wall
0.2973.16E-120SGTC_6631486-0733 13.9 μMChemDiv (Drug-like library)7224450.0864198cell wall signaling
0.2951.29E-118SGTC_8630466-0191 129.0 μMChemDiv (Drug-like library)7651600.138462cell wall
0.2959.33E-118SGTC_30601327-0027 12.5 μMChemDiv (Drug-like library)160004550.138889
0.2852.10E-110SGTC_5860957-0265 8.2 μMChemDiv (Drug-like library)67523090.103448cell wall
0.2832.84E-109SGTC_6420335-0876 158.0 μMChemDiv (Drug-like library)68123500.0649351ubiquinone biosynthesis & proteasome
0.2836.58E-109SGTC_33569151811 4.5 μMChembridge (Drug-like library)18592540.1375cell wall
0.2811.87E-107SGTC_9641287-0107 19.4 μMChemDiv (Drug-like library)515168120.0588235cell wall

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_23789074213121.43 μM0.354167657714Chembridge (Fragment library)195.215121.44213RSC complex & mRNA processing
SGTC_2796794963445.45 μM0.2985072971588Chembridge (Drug-like library)366.433523.11515
SGTC_9861493-048384 μM0.28070240079ChemDiv (Drug-like library)256.256681.31914
SGTC_1167k781-0610148 μM0.2786894525169ChemDiv (Drug-like library)276.314282.7934RNA processing & uracil transport
SGTC_2897904859812.99 μM0.2786896467404Chembridge (Drug-like library)283.321782.99113
SGTC_415dibenzyl ketone100 μM0.2727277593Miscellaneous210.271063.29401
SGTC_3232913287449.47 μM0.2727273784940Chembridge (Drug-like library)255.268622.4713RPP1 & pyrimidine depletion
SGTC_1685st02404953.09 μM0.2676061941139TimTec (Natural product derivative library)363.406443.35214TSC3-RPN4
SGTC_10234482-388816 μM0.2666673303168ChemDiv (Drug-like library)267.324062.58205TSC3-RPN4
SGTC_1759st04305970 μM0.266667669426TimTec (Natural product derivative library)285.29462.45314amide catabolism