paxilline

A toxic potassium channel blocker.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_489
Screen concentration 115.0 μM
Source ICCB bioactive library
PubChem CID 105008
SMILES CC12CCC3C(=CC(=O)C(O3)C(C)(C)O)C1(CCC4C2(C5=C(C4)C6=CC=CC=C6N5)C)O
Standardized SMILES CC(C)(O)C1OC2CCC3(C)C(O)(CCC4Cc5c([nH]c6ccccc56)C34C)C2=CC1=O
Molecular weight 435.5552
ALogP 3.9
H-bond donor count 3
H-bond acceptor count 4
Response signature PDR1

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.01
% growth inhibition (Hom. pool) 3.86


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 105008
Download HIP data (tab-delimited text)  (excel)
Gene:DPM1(YPR183W)|FD-Score:4.51|P-value:3.22E-6|Clearance:0.5||SGD DESC:Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation Gene:ERG9(YHR190W)|FD-Score:-3.29|P-value:5.09E-4|Clearance:0||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:GPI19(YDR437W)|FD-Score:-3.75|P-value:8.91E-5|Clearance:0||SGD DESC:Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P Gene:IPI1(YHR085W)|FD-Score:-3.61|P-value:1.55E-4|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; IPI1 is an essential gene Gene:PRP45(YAL032C)|FD-Score:-3.54|P-value:1.98E-4|Clearance:0||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:RRP7(YCL031C)|FD-Score:3.14|P-value:8.53E-4|Clearance:0.02||SGD DESC:Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress Gene:SEC13(YLR208W)|FD-Score:4.33|P-value:7.59E-6|Clearance:0.5||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SRB6(YBR253W)|FD-Score:3.22|P-value:6.31E-4|Clearance:0.09||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:SSL1(YLR005W)|FD-Score:3.82|P-value:6.58E-5|Clearance:0.29||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:SYF1(YDR416W)|FD-Score:3.12|P-value:9.17E-4|Clearance:0.03||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans Gene:TID3(YIL144W)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.3||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:DPM1(YPR183W)|FD-Score:4.51|P-value:3.22E-6|Clearance:0.5||SGD DESC:Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation Gene:ERG9(YHR190W)|FD-Score:-3.29|P-value:5.09E-4|Clearance:0||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:GPI19(YDR437W)|FD-Score:-3.75|P-value:8.91E-5|Clearance:0||SGD DESC:Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P Gene:IPI1(YHR085W)|FD-Score:-3.61|P-value:1.55E-4|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; IPI1 is an essential gene Gene:PRP45(YAL032C)|FD-Score:-3.54|P-value:1.98E-4|Clearance:0||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:RRP7(YCL031C)|FD-Score:3.14|P-value:8.53E-4|Clearance:0.02||SGD DESC:Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress Gene:SEC13(YLR208W)|FD-Score:4.33|P-value:7.59E-6|Clearance:0.5||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SRB6(YBR253W)|FD-Score:3.22|P-value:6.31E-4|Clearance:0.09||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:SSL1(YLR005W)|FD-Score:3.82|P-value:6.58E-5|Clearance:0.29||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:SYF1(YDR416W)|FD-Score:3.12|P-value:9.17E-4|Clearance:0.03||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans Gene:TID3(YIL144W)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.3||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 105008
Download HOP data (tab-delimited text)  (excel)
Gene:ADI1(YMR009W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions Gene:ARC18(YLR370C)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATP17(YDR377W)|FD-Score:3.79|P-value:7.57E-5||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:ATP22(YDR350C)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Specific translational activator for the mitochondrial ATP6 mRNA, encoding a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane Gene:BTS1(YPL069C)|FD-Score:7.15|P-value:4.38E-13||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUB2(YMR055C)|FD-Score:3.79|P-value:7.46E-5||SGD DESC:Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage Gene:CRH1(YGR189C)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:CTF8(YHR191C)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DOA4(YDR069C)|FD-Score:3.9|P-value:4.76E-5||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:DSE3(YOR264W)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ERG2(YMR202W)|FD-Score:3.78|P-value:7.81E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:EST2(YLR318W)|FD-Score:-3.17|P-value:7.73E-4||SGD DESC:Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia Gene:FDC1(YDR539W)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:FUR4(YBR021W)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:HEM14(YER014W)|FD-Score:-3.92|P-value:4.51E-5||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:IDS2(YJL146W)|FD-Score:4.43|P-value:4.75E-6||SGD DESC:Protein involved in modulation of Ime2p activity during meiosis, appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation Gene:IKI1(YHR187W)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:LSB3(YFR024C-A)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication Gene:MRPL37(YBR268W)|FD-Score:-3.16|P-value:7.94E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRS2(YOR334W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Mitochondrial inner membrane Mg(2+) channel, required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns Gene:MSH1(YHR120W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MSN1(YOL116W)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MUD1(YBR119W)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:NAM2(YLR382C)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NUM1(YDR150W)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:ORM1(YGR038W)|FD-Score:-4.27|P-value:9.82E-6||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:OSH2(YDL019C)|FD-Score:-3.79|P-value:7.56E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PCK1(YKR097W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PDR1(YGL013C)|FD-Score:12.4|P-value:2.32E-35||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PFK1(YGR240C)|FD-Score:5|P-value:2.89E-7||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PIH1(YHR034C)|FD-Score:-3.18|P-value:7.30E-4||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:POL4(YCR014C)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta Gene:PRR1(YKL116C)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:PTR2(YKR093W)|FD-Score:3.96|P-value:3.70E-5||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:PUF2(YPR042C)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:RAD18(YCR066W)|FD-Score:4.38|P-value:5.94E-6||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:REG1(YDR028C)|FD-Score:3.76|P-value:8.57E-5||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIM101(YHL027W)|FD-Score:-3.19|P-value:7.03E-4||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RPL16A(YIL133C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPN4(YDL020C)|FD-Score:5.08|P-value:1.86E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS14A(YCR031C)|FD-Score:-3.46|P-value:2.66E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RTN2(YDL204W)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:SED4(YCR067C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p Gene:SFM1(YOR021C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism Gene:SNA3(YJL151C)|FD-Score:-3.21|P-value:6.63E-4||SGD DESC:Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles Gene:SNF12(YNR023W)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SPT3(YDR392W)|FD-Score:3.76|P-value:8.48E-5||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRB8(YCR081W)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:STR2(YJR130C)|FD-Score:-3.11|P-value:9.29E-4||SGD DESC:Cystathionine gamma-synthase, converts cysteine into cystathionine Gene:TEA1(YOR337W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein Gene:THO2(YNL139C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:VID24(YBR105C)|FD-Score:3.96|P-value:3.76E-5||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VPS51(YKR020W)|FD-Score:3.79|P-value:7.62E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:YBL055C(YBL055C)|FD-Score:-3.88|P-value:5.29E-5||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBR056W(YBR056W_p)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YCL042W(YCL042W_p)|FD-Score:4.52|P-value:3.07E-6||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YCR085W(YCR085W_d)|FD-Score:4.64|P-value:1.78E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR100C(YCR100C_p)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Putative protein of unknown function Gene:YDL057W(YDL057W_p)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Putative protein of unknown function; YDL057W is not an essential gene Gene:YDL094C(YDL094C_d)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential Gene:YDL177C(YDL177C_p)|FD-Score:-4.34|P-value:7.08E-6||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YDR239C(YDR239C)|FD-Score:-3.31|P-value:4.66E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YER119C-A(YER119C-A_d)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YHR210C(YHR210C_p)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YIL001W(YIL001W_p)|FD-Score:3.87|P-value:5.55E-5||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YIP5(YGL161C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YMR027W(YMR027W_p)|FD-Score:-3.28|P-value:5.18E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene Gene:YNL024C(YNL024C_p)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Putative methyltransferase; has seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; despite similarity to methyltransferases, null mutant does not display alterations in lysine methylation pattern Gene:YOR034C-A(YOR034C-A_p)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR161C-C(YOR161C-C_p)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPK1(YKL126W)|FD-Score:5.18|P-value:1.13E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:ADI1(YMR009W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions Gene:ARC18(YLR370C)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATP17(YDR377W)|FD-Score:3.79|P-value:7.57E-5||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:ATP22(YDR350C)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Specific translational activator for the mitochondrial ATP6 mRNA, encoding a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane Gene:BTS1(YPL069C)|FD-Score:7.15|P-value:4.38E-13||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUB2(YMR055C)|FD-Score:3.79|P-value:7.46E-5||SGD DESC:Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage Gene:CRH1(YGR189C)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:CTF8(YHR191C)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DOA4(YDR069C)|FD-Score:3.9|P-value:4.76E-5||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:DSE3(YOR264W)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ERG2(YMR202W)|FD-Score:3.78|P-value:7.81E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:EST2(YLR318W)|FD-Score:-3.17|P-value:7.73E-4||SGD DESC:Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia Gene:FDC1(YDR539W)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:FUR4(YBR021W)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:HEM14(YER014W)|FD-Score:-3.92|P-value:4.51E-5||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:IDS2(YJL146W)|FD-Score:4.43|P-value:4.75E-6||SGD DESC:Protein involved in modulation of Ime2p activity during meiosis, appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation Gene:IKI1(YHR187W)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:LSB3(YFR024C-A)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication Gene:MRPL37(YBR268W)|FD-Score:-3.16|P-value:7.94E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRS2(YOR334W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Mitochondrial inner membrane Mg(2+) channel, required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns Gene:MSH1(YHR120W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MSN1(YOL116W)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MUD1(YBR119W)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:NAM2(YLR382C)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NUM1(YDR150W)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:ORM1(YGR038W)|FD-Score:-4.27|P-value:9.82E-6||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:OSH2(YDL019C)|FD-Score:-3.79|P-value:7.56E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PCK1(YKR097W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PDR1(YGL013C)|FD-Score:12.4|P-value:2.32E-35||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PFK1(YGR240C)|FD-Score:5|P-value:2.89E-7||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PIH1(YHR034C)|FD-Score:-3.18|P-value:7.30E-4||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:POL4(YCR014C)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta Gene:PRR1(YKL116C)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:PTR2(YKR093W)|FD-Score:3.96|P-value:3.70E-5||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:PUF2(YPR042C)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:RAD18(YCR066W)|FD-Score:4.38|P-value:5.94E-6||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:REG1(YDR028C)|FD-Score:3.76|P-value:8.57E-5||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIM101(YHL027W)|FD-Score:-3.19|P-value:7.03E-4||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RPL16A(YIL133C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPN4(YDL020C)|FD-Score:5.08|P-value:1.86E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS14A(YCR031C)|FD-Score:-3.46|P-value:2.66E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RTN2(YDL204W)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:SED4(YCR067C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p Gene:SFM1(YOR021C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism Gene:SNA3(YJL151C)|FD-Score:-3.21|P-value:6.63E-4||SGD DESC:Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles Gene:SNF12(YNR023W)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SPT3(YDR392W)|FD-Score:3.76|P-value:8.48E-5||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRB8(YCR081W)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:STR2(YJR130C)|FD-Score:-3.11|P-value:9.29E-4||SGD DESC:Cystathionine gamma-synthase, converts cysteine into cystathionine Gene:TEA1(YOR337W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein Gene:THO2(YNL139C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:VID24(YBR105C)|FD-Score:3.96|P-value:3.76E-5||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VPS51(YKR020W)|FD-Score:3.79|P-value:7.62E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:YBL055C(YBL055C)|FD-Score:-3.88|P-value:5.29E-5||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBR056W(YBR056W_p)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YCL042W(YCL042W_p)|FD-Score:4.52|P-value:3.07E-6||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YCR085W(YCR085W_d)|FD-Score:4.64|P-value:1.78E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR100C(YCR100C_p)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Putative protein of unknown function Gene:YDL057W(YDL057W_p)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Putative protein of unknown function; YDL057W is not an essential gene Gene:YDL094C(YDL094C_d)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential Gene:YDL177C(YDL177C_p)|FD-Score:-4.34|P-value:7.08E-6||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YDR239C(YDR239C)|FD-Score:-3.31|P-value:4.66E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YER119C-A(YER119C-A_d)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YHR210C(YHR210C_p)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YIL001W(YIL001W_p)|FD-Score:3.87|P-value:5.55E-5||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YIP5(YGL161C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YMR027W(YMR027W_p)|FD-Score:-3.28|P-value:5.18E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene Gene:YNL024C(YNL024C_p)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Putative methyltransferase; has seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; despite similarity to methyltransferases, null mutant does not display alterations in lysine methylation pattern Gene:YOR034C-A(YOR034C-A_p)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR161C-C(YOR161C-C_p)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPK1(YKL126W)|FD-Score:5.18|P-value:1.13E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR183W4.513.22E-60.50DPM1Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation
YLR208W4.337.59E-60.50SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YLR005W3.826.58E-50.29SSL1Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
YIL144W3.532.08E-40.30TID3Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering
YBR253W3.226.31E-40.09SRB6Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YCL031C3.148.53E-40.02RRP7Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress
YDR416W3.129.17E-40.03SYF1Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans
YMR076C3.090.001020.13PDS5Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis
YJL005W2.960.001540.02CYR1Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation
YPR176C2.940.001620.05BET2Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YPL124W2.900.001870.03SPC29Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication
YDL143W2.870.002030.15CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YER133W2.720.003230.00GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YDR141C2.720.003260.02DOP1Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies
YPR177C_d2.700.003450.11YPR177C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL013C12.402.32E-35PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YPL069C7.154.38E-13BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YKL126W5.181.13E-7YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YDL020C5.081.86E-7RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YGR240C5.002.89E-7PFK1Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes
YCR085W_d4.641.78E-6YCR085W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YCL042W_p4.523.07E-6YCL042W_pPutative protein of unknown function; epitope-tagged protein localizes to the cytoplasm
YJL146W4.434.75E-6IDS2Protein involved in modulation of Ime2p activity during meiosis, appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation
YCR066W4.385.94E-6RAD18E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
YCR081W4.211.26E-5SRB8Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression
YCR067C4.151.65E-5SED4Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p
YDL057W_p4.062.43E-5YDL057W_pPutative protein of unknown function; YDL057W is not an essential gene
YKR093W3.963.70E-5PTR2Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p
YBR105C3.963.76E-5VID24GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles
YDR069C3.904.76E-5DOA4Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole

GO enrichment analysis for SGTC_489
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2625.33E-93SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.0535714PDR1
0.2328.40E-73SGTC_1694st024011 44.9 μMTimTec (Natural product derivative library)27875330.117647ERG2
0.2301.11E-71SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.110092PDR1
0.2212.39E-66SGTC_295k015-0027 31.0 μMChemDiv (Drug-like library)38874240.0638298PDR1
0.2201.27E-65SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.117021ERG2
0.2132.54E-61SGTC_12430416-0003 5.7 μMChemDiv (Drug-like library)21419770.0886076PDR1
0.2057.31E-57SGTC_32419133421 49.5 μMChembridge (Drug-like library)170182210.0642202PDR1
0.1982.22E-53SGTC_1139k015-0024 46.9 μMChemDiv (Drug-like library)27666520.0888889PDR1
0.1971.44E-52SGTC_5983937-0236 20.2 μMChemDiv (Drug-like library)13793130.0531915ERG2
0.1924.13E-50SGTC_2508celastrol 40.5 μMMicrosource (Natural product library)1227240.116505PDR1
0.1911.12E-49SGTC_250brefeldin a 552.2 μMICCB bioactive library64361870.108696PDR1
0.1899.93E-49SGTC_6641222-0054 14.9 μMChemDiv (Drug-like library)68139910.0526316sphingolipid biosynthesis & PDR1
0.1822.88E-45SGTC_30259087895 49.5 μMChembridge (Drug-like library)171736410.0576923PDR1
0.1759.97E-42SGTC_12770873-0036 22.0 μMChemDiv (Drug-like library)59657350.0526316PDR1
0.1681.78E-38SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.0729167ERG2

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_20155119600184 μM0.270272828786Chembridge (Fragment library)200.236482.07521
SGTC_1993st07399570.8 μM0.2441863098394TimTec (Natural product derivative library)282.423144.94511
SGTC_1972st07020117.4 μM0.220932829099TimTec (Natural product derivative library)326.77691.10933
SGTC_1524corynanthine62.1 μM0.20618618528820TimTec (Pure natural product library)408.918962.99745
SGTC_1949st07602469.1 μM0.2045452921930TimTec (Natural product derivative library)289.3446032.56213
SGTC_1953st07680012.1 μM0.202703297591TimTec (Natural product derivative library)219.281124.53410
SGTC_1636st00770768.9 μM0.2403051TimTec (Natural product derivative library)290.359023.75911
SGTC_1954st07703268.9 μM0.2698907TimTec (Natural product derivative library)290.359023.68611
SGTC_25303-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene92.62 μM0.1756708760Microsource (Natural product library)216.31873.35611
SGTC_2545solidagenone89.61 μM0.1739136708572Microsource (Natural product library)316.434524.0113