capsazepine

N-[2-(4-chlorophenyl)ethyl]-7,8-dihydroxy-1,3,4,5-tetrahydro-2-benzazepine-2-carbothioamide

A calcium channel blocker.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_497
Screen concentration 133.0 μM
Source ICCB bioactive library
PubChem CID 2733484
SMILES C1CC2=CC(=C(C=C2CN(C1)C(=S)NCCC3=CC=C(C=C3)Cl)O)O
Standardized SMILES Oc1cc2CCCN(Cc2cc1O)C(=S)NCCc3ccc(Cl)cc3
Molecular weight 376.9002
ALogP 5.39
H-bond donor count 3
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.81
% growth inhibition (Hom. pool) 4.02


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2733484
Download HIP data (tab-delimited text)  (excel)
Gene:CDC19(YAL038W)|FD-Score:-3.83|P-value:6.38E-5|Clearance:0||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:CDC3(YLR314C)|FD-Score:-3.44|P-value:2.93E-4|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC9(YDL164C)|FD-Score:3.57|P-value:1.80E-4|Clearance:0||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:GCD2(YGR083C)|FD-Score:-3.78|P-value:7.80E-5|Clearance:0||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPI16(YHR188C)|FD-Score:4.2|P-value:1.34E-5|Clearance:0.55||SGD DESC:Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog Gene:IPP1(YBR011C)|FD-Score:6.38|P-value:8.91E-11|Clearance:1.43||SGD DESC:Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase Gene:NSE4(YDL105W)|FD-Score:3.11|P-value:9.28E-4|Clearance:0.12||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:NUP57(YGR119C)|FD-Score:3.59|P-value:1.63E-4|Clearance:0.03||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PKC1(YBL105C)|FD-Score:3.18|P-value:7.48E-4|Clearance:0.06||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRP46(YPL151C)|FD-Score:-3.58|P-value:1.69E-4|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:RPL10(YLR075W)|FD-Score:3.54|P-value:2.02E-4|Clearance:0.33||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPN12(YFR052W)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.06||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress Gene:RPN5(YDL147W)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.02||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPT6(YGL048C)|FD-Score:3.18|P-value:7.25E-4|Clearance:0.01||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RSC6(YCR052W)|FD-Score:4.95|P-value:3.64E-7|Clearance:0.07||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC16(YPL085W)|FD-Score:3.54|P-value:1.99E-4|Clearance:0||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:SEC6(YIL068C)|FD-Score:-3.17|P-value:7.52E-4|Clearance:0||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:SLN1(YIL147C)|FD-Score:3.21|P-value:6.69E-4|Clearance:0.02||SGD DESC:Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators Gene:SMD3(YLR147C)|FD-Score:-3.12|P-value:8.94E-4|Clearance:0||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:UTP5(YDR398W)|FD-Score:4.88|P-value:5.25E-7|Clearance:0.68||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:CDC19(YAL038W)|FD-Score:-3.83|P-value:6.38E-5|Clearance:0||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:CDC3(YLR314C)|FD-Score:-3.44|P-value:2.93E-4|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC9(YDL164C)|FD-Score:3.57|P-value:1.80E-4|Clearance:0||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:GCD2(YGR083C)|FD-Score:-3.78|P-value:7.80E-5|Clearance:0||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPI16(YHR188C)|FD-Score:4.2|P-value:1.34E-5|Clearance:0.55||SGD DESC:Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog Gene:IPP1(YBR011C)|FD-Score:6.38|P-value:8.91E-11|Clearance:1.43||SGD DESC:Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase Gene:NSE4(YDL105W)|FD-Score:3.11|P-value:9.28E-4|Clearance:0.12||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:NUP57(YGR119C)|FD-Score:3.59|P-value:1.63E-4|Clearance:0.03||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PKC1(YBL105C)|FD-Score:3.18|P-value:7.48E-4|Clearance:0.06||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRP46(YPL151C)|FD-Score:-3.58|P-value:1.69E-4|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:RPL10(YLR075W)|FD-Score:3.54|P-value:2.02E-4|Clearance:0.33||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPN12(YFR052W)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.06||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress Gene:RPN5(YDL147W)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.02||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPT6(YGL048C)|FD-Score:3.18|P-value:7.25E-4|Clearance:0.01||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RSC6(YCR052W)|FD-Score:4.95|P-value:3.64E-7|Clearance:0.07||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC16(YPL085W)|FD-Score:3.54|P-value:1.99E-4|Clearance:0||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:SEC6(YIL068C)|FD-Score:-3.17|P-value:7.52E-4|Clearance:0||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:SLN1(YIL147C)|FD-Score:3.21|P-value:6.69E-4|Clearance:0.02||SGD DESC:Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators Gene:SMD3(YLR147C)|FD-Score:-3.12|P-value:8.94E-4|Clearance:0||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:UTP5(YDR398W)|FD-Score:4.88|P-value:5.25E-7|Clearance:0.68||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2733484
Download HOP data (tab-delimited text)  (excel)
Gene:ACO2(YJL200C)|FD-Score:-4.08|P-value:2.30E-5||SGD DESC:Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol Gene:AIM19(YIL087C)|FD-Score:3.79|P-value:7.60E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth Gene:AIM5(YBR262C)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:APE3(YBR286W)|FD-Score:3.82|P-value:6.77E-5||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:BDH1(YAL060W)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:BIO3(YNR058W)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:BMH2(YDR099W)|FD-Score:-3.77|P-value:8.05E-5||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BNA3(YJL060W)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BRP1(YGL007W_d)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BUD27(YFL023W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CAN1(YEL063C)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CDH1(YGL003C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:DID2(YKR035W-A)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors Gene:DPP1(YDR284C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:ECM25(YJL201W)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:GLG2(YJL137C)|FD-Score:-4.56|P-value:2.51E-6||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:GNP1(YDR508C)|FD-Score:-6.05|P-value:7.13E-10||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:GUT1(YHL032C)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HKR1(YDR420W)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection Gene:HMX1(YLR205C)|FD-Score:-3.95|P-value:3.96E-5||SGD DESC:ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants Gene:MFA1(YDR461W)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 Gene:MOT3(YMR070W)|FD-Score:3.77|P-value:8.06E-5||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MPC54(YOR177C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MRPL37(YBR268W)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NPP2(YEL016C)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:OPI8(YKR035C_d)|FD-Score:3.28|P-value:5.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:OPI9(YLR338W_d)|FD-Score:-4.2|P-value:1.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PKP2(YGL059W)|FD-Score:-3.78|P-value:7.98E-5||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:PML1(YLR016C)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p Gene:PTC6(YCR079W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:RDS2(YPL133C)|FD-Score:4.31|P-value:8.34E-6||SGD DESC:Transcription factor involved in regulating gluconeogenesis and glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole Gene:REC104(YHR157W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RGC1(YPR115W)|FD-Score:-3.27|P-value:5.36E-4||SGD DESC:Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication Gene:RIT1(YMR283C)|FD-Score:-3.18|P-value:7.41E-4||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:RMD8(YFR048W)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Cytosolic protein required for sporulation Gene:RNY1(YPL123C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity Gene:RPL9A(YGL147C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RPS10B(YMR230W)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:RPS16A(YMR143W)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:RPS28B(YLR264W)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:SBH1(YER087C-B)|FD-Score:-3.13|P-value:8.73E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SLT2(YHR030C)|FD-Score:-4.73|P-value:1.14E-6||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:TGL2(YDR058C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:THI20(YOL055C)|FD-Score:4.9|P-value:4.74E-7||SGD DESC:Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p Gene:TRM732(YMR259C_p)|FD-Score:4.49|P-value:3.48E-6||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:TRP3(YKL211C)|FD-Score:6.37|P-value:9.52E-11||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TWF1(YGR080W)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Twinfilin, highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton, composed of two cofilin-like regions, localizes actin monomers to sites of rapid filament assembly Gene:UBC12(YLR306W)|FD-Score:-3.1|P-value:9.66E-4||SGD DESC:Enzyme that mediates the conjugation of Rub1p, a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes Gene:UBX2(YML013W)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:UMP1(YBR173C)|FD-Score:3.77|P-value:8.25E-5||SGD DESC:Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly Gene:VMA5(YKL080W)|FD-Score:-3.83|P-value:6.51E-5||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS24(YKL041W)|FD-Score:4.78|P-value:8.74E-7||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS72(YDR485C)|FD-Score:-3.25|P-value:5.80E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YCR007C(YCR007C_p)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDR186C(YDR186C)|FD-Score:3.79|P-value:7.40E-5||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGL039W(YGL039W)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Oxidoreductase shown to reduce carbonyl compounds to chiral alcohols Gene:YGL072C(YGL072C_d)|FD-Score:-3.77|P-value:8.16E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR219W(YGR219W_d)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YJL206C(YJL206C_p)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment Gene:YLR036C(YLR036C_p)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Putative protein of unknown function predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein Gene:YLR202C(YLR202C_d)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YMD8(YML038C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YMR172C-A(YMR172C-A_d)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR012W(YOR012W_p)|FD-Score:-4.3|P-value:8.42E-6||SGD DESC:Putative protein of unknown function Gene:YOR114W(YOR114W_p)|FD-Score:4.27|P-value:9.80E-6||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YOR225W(YOR225W_d)|FD-Score:-3.14|P-value:8.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR365C(YOR365C_p)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Putative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication Gene:YPR050C(YPR050C_d)|FD-Score:3.87|P-value:5.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPT32(YGL210W)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication Gene:ACO2(YJL200C)|FD-Score:-4.08|P-value:2.30E-5||SGD DESC:Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol Gene:AIM19(YIL087C)|FD-Score:3.79|P-value:7.60E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth Gene:AIM5(YBR262C)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:APE3(YBR286W)|FD-Score:3.82|P-value:6.77E-5||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:BDH1(YAL060W)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:BIO3(YNR058W)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:BMH2(YDR099W)|FD-Score:-3.77|P-value:8.05E-5||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BNA3(YJL060W)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BRP1(YGL007W_d)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BUD27(YFL023W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CAN1(YEL063C)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CDH1(YGL003C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:DID2(YKR035W-A)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors Gene:DPP1(YDR284C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:ECM25(YJL201W)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:GLG2(YJL137C)|FD-Score:-4.56|P-value:2.51E-6||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:GNP1(YDR508C)|FD-Score:-6.05|P-value:7.13E-10||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:GUT1(YHL032C)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HKR1(YDR420W)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection Gene:HMX1(YLR205C)|FD-Score:-3.95|P-value:3.96E-5||SGD DESC:ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants Gene:MFA1(YDR461W)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 Gene:MOT3(YMR070W)|FD-Score:3.77|P-value:8.06E-5||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MPC54(YOR177C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MRPL37(YBR268W)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NPP2(YEL016C)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:OPI8(YKR035C_d)|FD-Score:3.28|P-value:5.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:OPI9(YLR338W_d)|FD-Score:-4.2|P-value:1.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PKP2(YGL059W)|FD-Score:-3.78|P-value:7.98E-5||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:PML1(YLR016C)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p Gene:PTC6(YCR079W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:RDS2(YPL133C)|FD-Score:4.31|P-value:8.34E-6||SGD DESC:Transcription factor involved in regulating gluconeogenesis and glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole Gene:REC104(YHR157W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RGC1(YPR115W)|FD-Score:-3.27|P-value:5.36E-4||SGD DESC:Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication Gene:RIT1(YMR283C)|FD-Score:-3.18|P-value:7.41E-4||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:RMD8(YFR048W)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Cytosolic protein required for sporulation Gene:RNY1(YPL123C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity Gene:RPL9A(YGL147C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RPS10B(YMR230W)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:RPS16A(YMR143W)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:RPS28B(YLR264W)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:SBH1(YER087C-B)|FD-Score:-3.13|P-value:8.73E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SLT2(YHR030C)|FD-Score:-4.73|P-value:1.14E-6||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:TGL2(YDR058C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:THI20(YOL055C)|FD-Score:4.9|P-value:4.74E-7||SGD DESC:Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p Gene:TRM732(YMR259C_p)|FD-Score:4.49|P-value:3.48E-6||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:TRP3(YKL211C)|FD-Score:6.37|P-value:9.52E-11||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TWF1(YGR080W)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Twinfilin, highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton, composed of two cofilin-like regions, localizes actin monomers to sites of rapid filament assembly Gene:UBC12(YLR306W)|FD-Score:-3.1|P-value:9.66E-4||SGD DESC:Enzyme that mediates the conjugation of Rub1p, a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes Gene:UBX2(YML013W)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:UMP1(YBR173C)|FD-Score:3.77|P-value:8.25E-5||SGD DESC:Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly Gene:VMA5(YKL080W)|FD-Score:-3.83|P-value:6.51E-5||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS24(YKL041W)|FD-Score:4.78|P-value:8.74E-7||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS72(YDR485C)|FD-Score:-3.25|P-value:5.80E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YCR007C(YCR007C_p)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDR186C(YDR186C)|FD-Score:3.79|P-value:7.40E-5||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGL039W(YGL039W)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Oxidoreductase shown to reduce carbonyl compounds to chiral alcohols Gene:YGL072C(YGL072C_d)|FD-Score:-3.77|P-value:8.16E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR219W(YGR219W_d)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YJL206C(YJL206C_p)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment Gene:YLR036C(YLR036C_p)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Putative protein of unknown function predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein Gene:YLR202C(YLR202C_d)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YMD8(YML038C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YMR172C-A(YMR172C-A_d)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR012W(YOR012W_p)|FD-Score:-4.3|P-value:8.42E-6||SGD DESC:Putative protein of unknown function Gene:YOR114W(YOR114W_p)|FD-Score:4.27|P-value:9.80E-6||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YOR225W(YOR225W_d)|FD-Score:-3.14|P-value:8.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR365C(YOR365C_p)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Putative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication Gene:YPR050C(YPR050C_d)|FD-Score:3.87|P-value:5.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPT32(YGL210W)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YBR011C6.388.91E-111.43IPP1Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase
YCR052W4.953.64E-70.07RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YDR398W4.885.25E-70.68UTP5Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YHR188C4.201.34E-50.55GPI16Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog
YFR052W3.651.31E-40.06RPN12Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress
YGR119C3.591.63E-40.03NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
YDL164C3.571.80E-40.00CDC9DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination
YDL147W3.571.81E-40.03RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YPL085W3.541.99E-40.00SEC16COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p
YLR075W3.542.02E-40.33RPL10Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects
YIL147C3.216.69E-40.02SLN1Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators
YGL048C3.187.25E-40.01RPT6ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
YBL105C3.187.48E-40.06PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YDL105W3.119.28E-40.12NSE4Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
YOR260W3.000.001370.07GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL211C6.379.52E-11TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YOL055C4.904.74E-7THI20Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p
YKL041W4.788.74E-7VPS24One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway
YCR007C_p4.731.11E-6YCR007C_pPutative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene
YMR259C_p4.493.48E-6TRM732_pInteracts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene
YPL133C4.318.34E-6RDS2Transcription factor involved in regulating gluconeogenesis and glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole
YOR114W_p4.279.80E-6YOR114W_pPutative protein of unknown function; null mutant is viable
YML038C4.241.10E-5YMD8Putative nucleotide sugar transporter, has similarity to Vrg4p
YMR143W4.121.88E-5RPS16AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication
YGL039W4.062.44E-5YGL039WOxidoreductase shown to reduce carbonyl compounds to chiral alcohols
YPR050C_d3.875.42E-5YPR050C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W
YNR058W3.826.69E-5BIO37,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis
YBR286W3.826.77E-5APE3Vacuolar aminopeptidase Y, processed to mature form by Prb1p
YDR186C3.797.40E-5YDR186CPutative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YIL087C3.797.60E-5AIM19Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth

GO enrichment analysis for SGTC_497
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0985.02E-14SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.046875
0.0952.86E-13SGTC_2409alverine citrate 64.0 μMMiscellaneous217180.114754
0.0872.13E-11SGTC_11931488-0126 41.5 μMChemDiv (Drug-like library)13847100.12
0.0857.48E-11SGTC_15465-methoxyflavanone 78.7 μMTimTec (Pure natural product library)1477950.0519481
0.0822.59E-10SGTC_14964469-0713 57.4 μMChemDiv (Drug-like library)7466910.0595238
0.0822.59E-10SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.0493827
0.0815.19E-10SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.146667
0.0807.41E-10SGTC_22246650123 117.6 μMChembridge (Fragment library)29083700.102564
0.0808.17E-10SGTC_501nsc-95397 161.0 μMICCB bioactive library2620930.078125
0.0772.65E-9SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.130435TSC3-RPN4
0.0773.54E-9SGTC_33189139014 38.6 μMChembridge (Drug-like library)166439840.0886076
0.0773.76E-9SGTC_11941488-0194 225.0 μMChemDiv (Drug-like library)13914520.0506329
0.0741.09E-8SGTC_8191013-0258 27.7 μMChemDiv (Drug-like library)57531980.15
0.0731.66E-8SGTC_18815652484 16.0 μMMiscellaneous22551290.0804598TRP & mitochondrial translation
0.0722.70E-8SGTC_11720370-0043 85.0 μMChemDiv (Drug-like library)3765850.125

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_6530203-001655.2 μM0.290323295302ChemDiv (Drug-like library)289.756743.44212
SGTC_3033909088749.47 μM0.2812519325384Chembridge (Drug-like library)342.862423.9402fatty acid desaturase (OLE1)
SGTC_3084092-1169104 μM0.2769232268590ChemDiv (Drug-like library)346.851123.8514plasma membrane duress
SGTC_6681682-049962.6 μM0.272727682214ChemDiv (Drug-like library)324.20184.10612
SGTC_1835st00866649.2 μM0.265625690455TimTec (Natural product derivative library)306.186524.2301
SGTC_5154092-1064102 μM0.258621717353ChemDiv (Drug-like library)335.27084.77302plasma membrane duress
SGTC_2835900658871.43 μM0.2575762345538Chembridge (Drug-like library)300.782683.27912
SGTC_2784092-082123.9 μM0.25877900ChemDiv (Drug-like library)328.87895.05102plasma membrane duress
SGTC_1770st04388466.3 μM0.246377670149TimTec (Natural product derivative library)301.767443.5502
SGTC_11594092-053861.2 μM0.246154717301ChemDiv (Drug-like library)332.7997233.65403