genistein

5,7-dimethyl-4-methylidene-3-(4-methylphenyl)-1H-naphthalene

A kinase inhibitor, peroxisome proliferator-activated receptor activator and oxygen scavenger.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_499
Screen concentration 192.0 μM
Source ICCB bioactive library
PubChem CID 6610345
SMILES CC1=CC=C(C=C1)C2=CCC3=CC(=CC(=C3C2=C)C)C
Standardized SMILES Cc1ccc(cc1)C2=CCc3cc(C)cc(C)c3C2=C
Molecular weight 260.3728
ALogP 5.89
H-bond donor count 0
H-bond acceptor count 0
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.75
% growth inhibition (Hom. pool) 4.02


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6610345
Download HIP data (tab-delimited text)  (excel)
Gene:RFC2(YJR068W)|FD-Score:4.29|P-value:8.96E-6|Clearance:0.57||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:SUB2(YDL084W)|FD-Score:3.9|P-value:4.87E-5|Clearance:0.57||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:YDL152W(YDL152W_d)|FD-Score:3.52|P-value:2.19E-4|Clearance:0.57||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:RFC2(YJR068W)|FD-Score:4.29|P-value:8.96E-6|Clearance:0.57||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:SUB2(YDL084W)|FD-Score:3.9|P-value:4.87E-5|Clearance:0.57||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:YDL152W(YDL152W_d)|FD-Score:3.52|P-value:2.19E-4|Clearance:0.57||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6610345
Download HOP data (tab-delimited text)  (excel)
Gene:GAL83(YER027C)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:GUP2(YPL189W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog Gene:HSL7(YBR133C)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:NMA1(YLR328W)|FD-Score:3.75|P-value:8.99E-5||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:YDL218W(YDL218W_p)|FD-Score:-3.13|P-value:8.80E-4||SGD DESC:Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin Gene:YDR008C(YDR008C_d)|FD-Score:-3.11|P-value:9.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFL042C(YFL042C_p)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YGR182C(YGR182C_d)|FD-Score:-3.1|P-value:9.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TIM13/YGR181W Gene:YKL102C(YKL102C_d)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site Gene:YOR152C(YOR152C_p)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Putative protein of unknown function; YOR152C is not an essential gene Gene:GAL83(YER027C)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:GUP2(YPL189W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog Gene:HSL7(YBR133C)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:NMA1(YLR328W)|FD-Score:3.75|P-value:8.99E-5||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:YDL218W(YDL218W_p)|FD-Score:-3.13|P-value:8.80E-4||SGD DESC:Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin Gene:YDR008C(YDR008C_d)|FD-Score:-3.11|P-value:9.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFL042C(YFL042C_p)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YGR182C(YGR182C_d)|FD-Score:-3.1|P-value:9.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TIM13/YGR181W Gene:YKL102C(YKL102C_d)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site Gene:YOR152C(YOR152C_p)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Putative protein of unknown function; YOR152C is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJR068W4.298.96E-60.57RFC2Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YDL084W3.904.87E-50.57SUB2Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress
YDL152W_d3.522.19E-40.57YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YOR257W2.950.001590.04CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YOR207C2.910.001830.01RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YML105C2.890.001910.20SEC65Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19
YGL097W2.700.003520.07SRM1Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p
YDL195W2.620.004390.04SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YKL144C2.580.004870.03RPC25RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p
YIL144W2.560.005300.17TID3Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering
YLR141W2.380.008608.34E-4RRN5Protein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p
YFL038C2.380.008620.01YPT1Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
YGR190C_d2.370.008800.04YGR190C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W
YDL150W2.330.009890.01RPC53RNA polymerase III subunit C53
YBR038W2.320.010000.03CHS2Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL102C_d4.221.21E-5YKL102C_dDubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site
YDL020C4.181.43E-5RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YLR328W3.758.99E-5NMA1Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication
YFL042C_p3.561.88E-4YFL042C_pPutative protein of unknown function; YFL042C is not an essential gene
YPL189W3.512.27E-4GUP2Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog
YIL145C3.050.00115PAN6Pantothenate synthase, also known as pantoate-beta-alanine ligase, required for pantothenic acid biosynthesis, deletion causes pantothenic acid auxotrophy, homologous to E. coli panC
YML102W3.000.00135CAC2Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription
YDL018C3.000.00135ERP3Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
YDR248C_p2.990.00141YDR248C_pPutative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1
YJR031C2.950.00158GEA1Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication
YDR503C2.950.00161LPP1Lipid phosphate phosphatase, catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA
YJR110W2.930.00168YMR1Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family
YDR046C2.890.00194BAP3Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication
YLR151C2.860.00210PCD1Peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member
YJL045W2.830.00230YJL045WMinor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner

GO enrichment analysis for SGTC_499
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1653.27E-37SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.0517241
0.1529.51E-32SGTC_32799140807 49.5 μMChembridge (Drug-like library)455957180.0714286
0.1411.34E-27SGTC_508roscovitine 141.0 μMICCB bioactive library1603550.0512821
0.1411.39E-27SGTC_23859074692 200.0 μMChembridge (Fragment library)170469060.08
0.1271.36E-22SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.0517241
0.1181.12E-19SGTC_30259087895 49.5 μMChembridge (Drug-like library)171736410.140845PDR1
0.1165.17E-19SGTC_1165k072-0275 30.3 μMChemDiv (Drug-like library)61535230.101695
0.1158.30E-19SGTC_2499avocadenofuran 51.5 μMMicrosource (Natural product library)68577920.0727273
0.1141.20E-18SGTC_575k072-0246 11.9 μMChemDiv (Drug-like library)67967790.0533333
0.1126.76E-18SGTC_14173966-0327 90.4 μMChemDiv (Drug-like library)6511180.056338
0.1056.85E-16SGTC_12870971-0001 34.6 μMChemDiv (Drug-like library)46658690.118644sphingolipid biosynthesis & PDR1
0.1032.06E-15SGTC_2248tofa 1.1 μMMiscellaneous1151750.0655738sphingolipid biosynthesis & PDR1
0.1023.80E-15SGTC_64000l-0018 272.1 μMChemDiv (Drug-like library)68111190.0657895ubiquinone biosynthesis & proteasome
0.1018.50E-15SGTC_7033875-0075 71.8 μMChemDiv (Drug-like library)28508140.0632911
0.0983.41E-14SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.046875

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3328914062429.04 μM0.2166674904392Chembridge (Drug-like library)335.4463.33104
SGTC_3043909230349.47 μM0.21428625236084Chembridge (Drug-like library)299.430443.97302
SGTC_23809035439200 μM0.2127666462575Chembridge (Fragment library)201.693142.33922endomembrane recycling
SGTC_3088911757849.47 μM0.211538678341Chembridge (Drug-like library)251.279924.11303amide catabolism
SGTC_1811st05006961.5 μM0.23877702TimTec (Natural product derivative library)325.444584.69113
SGTC_3257913920632.98 μM0.1967214910041Chembridge (Drug-like library)321.419423.04804
SGTC_255413-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one66.67 μM0.1929826708561Microsource (Natural product library)228.32943.65701
SGTC_270485-000132.62 μM0.1923082054479ChemDiv (Drug-like library)266.360764.32313RPP1 & pyrimidine depletion
SGTC_14554262-0120131 μM0.1914891529587ChemDiv (Drug-like library)308.460424.87302plasma membrane duress
SGTC_1691st01993384.6 μM0.1886796176082TimTec (Natural product derivative library)236.308344.67401