tyrphostin 8

2-[(4-hydroxyphenyl)methylidene]propanedinitrile

A tyrosine kinase inhibitor.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_503
Screen concentration 294.0 μM
Source ICCB bioactive library
PubChem CID 92970
SMILES C1=CC(=CC=C1C=C(C#N)C#N)O
Standardized SMILES Oc1ccc(C=C(C#N)C#N)cc1
Molecular weight 170.1674
ALogP 1.77
H-bond donor count 1
H-bond acceptor count 3
Response signature TSC3-RPN4

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.57
% growth inhibition (Hom. pool) 8.45


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 92970
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:-3.82|P-value:6.78E-5|Clearance:0||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:COG4(YPR105C)|FD-Score:-3.74|P-value:9.23E-5|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:GWT1(YJL091C)|FD-Score:3.4|P-value:3.39E-4|Clearance:0.3||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:NUS1(YDL193W)|FD-Score:3.53|P-value:2.11E-4|Clearance:0.13||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PRE2(YPR103W)|FD-Score:3.9|P-value:4.83E-5|Clearance:0.37||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RER2(YBR002C)|FD-Score:8.11|P-value:2.49E-16|Clearance:2.4||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RET1(YOR207C)|FD-Score:15|P-value:6.73E-51|Clearance:6.38||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPB8(YOR224C)|FD-Score:4.87|P-value:5.63E-7|Clearance:0.5||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC53(YDL150W)|FD-Score:5.41|P-value:3.11E-8|Clearance:0.54||SGD DESC:RNA polymerase III subunit C53 Gene:RPP1(YHR062C)|FD-Score:8.58|P-value:4.81E-18|Clearance:0.47||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SRM1(YGL097W)|FD-Score:4.37|P-value:6.27E-6|Clearance:0.03||SGD DESC:Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p Gene:TOM20(YGR082W)|FD-Score:3.09|P-value:9.88E-4|Clearance:0.07||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins Gene:YDL152W(YDL152W_d)|FD-Score:5.71|P-value:5.65E-9|Clearance:0.3||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YDR526C(YDR526C_d)|FD-Score:4.34|P-value:7.25E-6|Clearance:0.44||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR076C(YLR076C_d)|FD-Score:-5.08|P-value:1.89E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:ACC1(YNR016C)|FD-Score:-3.82|P-value:6.78E-5|Clearance:0||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:COG4(YPR105C)|FD-Score:-3.74|P-value:9.23E-5|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:GWT1(YJL091C)|FD-Score:3.4|P-value:3.39E-4|Clearance:0.3||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:NUS1(YDL193W)|FD-Score:3.53|P-value:2.11E-4|Clearance:0.13||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PRE2(YPR103W)|FD-Score:3.9|P-value:4.83E-5|Clearance:0.37||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RER2(YBR002C)|FD-Score:8.11|P-value:2.49E-16|Clearance:2.4||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RET1(YOR207C)|FD-Score:15|P-value:6.73E-51|Clearance:6.38||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPB8(YOR224C)|FD-Score:4.87|P-value:5.63E-7|Clearance:0.5||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC53(YDL150W)|FD-Score:5.41|P-value:3.11E-8|Clearance:0.54||SGD DESC:RNA polymerase III subunit C53 Gene:RPP1(YHR062C)|FD-Score:8.58|P-value:4.81E-18|Clearance:0.47||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SRM1(YGL097W)|FD-Score:4.37|P-value:6.27E-6|Clearance:0.03||SGD DESC:Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p Gene:TOM20(YGR082W)|FD-Score:3.09|P-value:9.88E-4|Clearance:0.07||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins Gene:YDL152W(YDL152W_d)|FD-Score:5.71|P-value:5.65E-9|Clearance:0.3||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YDR526C(YDR526C_d)|FD-Score:4.34|P-value:7.25E-6|Clearance:0.44||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR076C(YLR076C_d)|FD-Score:-5.08|P-value:1.89E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 92970
Download HOP data (tab-delimited text)  (excel)
Gene:AEP2(YMR282C)|FD-Score:4|P-value:3.13E-5||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AIM19(YIL087C)|FD-Score:3.72|P-value:9.96E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth Gene:AIM37(YNL100W)|FD-Score:-3.11|P-value:9.29E-4||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:APM4(YOL062C)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:ARG1(YOL058W)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate Gene:ARO3(YDR035W)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ARO7(YPR060C)|FD-Score:5.19|P-value:1.05E-7||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATP3(YBR039W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BMH2(YDR099W)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BST1(YFL025C)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUD27(YFL023W)|FD-Score:4.43|P-value:4.65E-6||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:BUL2(YML111W)|FD-Score:3.94|P-value:4.01E-5||SGD DESC:Component of the Rsp5p E3-ubiquitin ligase complex; involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication Gene:CBP2(YHL038C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene Gene:CDC26(YFR036W)|FD-Score:5.48|P-value:2.11E-8||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CIN4(YMR138W)|FD-Score:6.65|P-value:1.48E-11||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CLN3(YAL040C)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:CUE1(YMR264W)|FD-Score:-3.23|P-value:6.14E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DCS1(YLR270W)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DIN7(YDR263C)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:DRS2(YAL026C)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:DTR1(YBR180W)|FD-Score:4.49|P-value:3.58E-6||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:FET4(YMR319C)|FD-Score:-3.17|P-value:7.75E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GCN4(YEL009C)|FD-Score:3.74|P-value:9.38E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GEP5(YLR091W)|FD-Score:3.82|P-value:6.55E-5||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:GTT2(YLL060C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HAP3(YBL021C)|FD-Score:-3.12|P-value:9.00E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding Gene:HXT8(YJL214W)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:ISM1(YPL040C)|FD-Score:3.78|P-value:7.78E-5||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:LTE1(YAL024C)|FD-Score:3.9|P-value:4.76E-5||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MIG3(YER028C)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication Gene:MRP1(YDR347W)|FD-Score:3.75|P-value:8.82E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein Gene:MRP2(YPR166C)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRP4(YHL004W)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL11(YDL202W)|FD-Score:4.62|P-value:1.95E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL28(YDR462W)|FD-Score:3.86|P-value:5.70E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL33(YMR286W)|FD-Score:3.76|P-value:8.33E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL49(YJL096W)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL7(YDR237W)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MRPS35(YGR165W)|FD-Score:5.55|P-value:1.42E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MRPS5(YBR251W)|FD-Score:4.64|P-value:1.78E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSE1(YOL033W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:MST1(YKL194C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:PET112(YBL080C)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PET130(YJL023C)|FD-Score:5.41|P-value:3.12E-8||SGD DESC:Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PEX22(YAL055W)|FD-Score:-3.09|P-value:9.87E-4||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:QCR7(YDR529C)|FD-Score:3.83|P-value:6.49E-5||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RMD1(YDL001W)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Cytoplasmic protein required for sporulation Gene:RML2(YEL050C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RNH70(YGR276C)|FD-Score:5.65|P-value:7.94E-9||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:RNR3(YIL066C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Minor isoform of the large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits Gene:RPL13B(YMR142C)|FD-Score:7.42|P-value:5.83E-14||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS27B(YHR021C)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication Gene:RRG9(YNL213C)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RSM22(YKL155C)|FD-Score:5.85|P-value:2.48E-9||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RTC6(YPL183W-A)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SAC7(YDR389W)|FD-Score:-3.16|P-value:7.96E-4||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SED4(YCR067C)|FD-Score:5.19|P-value:1.05E-7||SGD DESC:Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p Gene:SKS1(YPL026C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Putative serine/threonine protein kinase; involved in the adaptation to low concentrations of glucose independent of the SNF3 regulated pathway Gene:SWM1(YDR260C)|FD-Score:4.66|P-value:1.62E-6||SGD DESC:Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation Gene:SWS2(YNL081C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TAT1(YBR069C)|FD-Score:-3.83|P-value:6.30E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA5(YLR426W_p)|FD-Score:-4.87|P-value:5.64E-7||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TOS6(YNL300W_p)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TRM3(YDL112W)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:TRP4(YDR354W)|FD-Score:-3.75|P-value:8.98E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:15.5|P-value:1.43E-54||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URE2(YNL229C)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VPS24(YKL041W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS62(YGR141W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins Gene:VPS8(YAL002W)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:YAL066W(YAL066W_d)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR116C(YBR116C_d)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TKL2 Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-4.48|P-value:3.81E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR102C(YDR102C_d)|FD-Score:4.39|P-value:5.65E-6||SGD DESC:Dubious open reading frame; homozygous diploid deletion strain exhibits high budding index Gene:YDR290W(YDR290W_d)|FD-Score:-3.37|P-value:3.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YEL010W(YEL010W_d)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER039C-A(YER039C-A_p)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Putative protein of unknown function; YER039C-A is not an essential gene Gene:YJR037W(YJR037W_d)|FD-Score:3.94|P-value:4.13E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces Gene:YKL102C(YKL102C_d)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site Gene:YLR118C(YLR118C)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YMD8(YML038C)|FD-Score:3.82|P-value:6.65E-5||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YML100W-A(YML100W-A_p)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YTA12(YMR089C)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes Gene:YTA7(YGR270W)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:ZRC1(YMR243C)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication Gene:AEP2(YMR282C)|FD-Score:4|P-value:3.13E-5||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AIM19(YIL087C)|FD-Score:3.72|P-value:9.96E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth Gene:AIM37(YNL100W)|FD-Score:-3.11|P-value:9.29E-4||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:APM4(YOL062C)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:ARG1(YOL058W)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate Gene:ARO3(YDR035W)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ARO7(YPR060C)|FD-Score:5.19|P-value:1.05E-7||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATP3(YBR039W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BMH2(YDR099W)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BST1(YFL025C)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUD27(YFL023W)|FD-Score:4.43|P-value:4.65E-6||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:BUL2(YML111W)|FD-Score:3.94|P-value:4.01E-5||SGD DESC:Component of the Rsp5p E3-ubiquitin ligase complex; involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication Gene:CBP2(YHL038C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene Gene:CDC26(YFR036W)|FD-Score:5.48|P-value:2.11E-8||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CIN4(YMR138W)|FD-Score:6.65|P-value:1.48E-11||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CLN3(YAL040C)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:CUE1(YMR264W)|FD-Score:-3.23|P-value:6.14E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DCS1(YLR270W)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DIN7(YDR263C)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:DRS2(YAL026C)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:DTR1(YBR180W)|FD-Score:4.49|P-value:3.58E-6||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:FET4(YMR319C)|FD-Score:-3.17|P-value:7.75E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GCN4(YEL009C)|FD-Score:3.74|P-value:9.38E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GEP5(YLR091W)|FD-Score:3.82|P-value:6.55E-5||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:GTT2(YLL060C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HAP3(YBL021C)|FD-Score:-3.12|P-value:9.00E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding Gene:HXT8(YJL214W)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:ISM1(YPL040C)|FD-Score:3.78|P-value:7.78E-5||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:LTE1(YAL024C)|FD-Score:3.9|P-value:4.76E-5||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MIG3(YER028C)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication Gene:MRP1(YDR347W)|FD-Score:3.75|P-value:8.82E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein Gene:MRP2(YPR166C)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRP4(YHL004W)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL11(YDL202W)|FD-Score:4.62|P-value:1.95E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL28(YDR462W)|FD-Score:3.86|P-value:5.70E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL33(YMR286W)|FD-Score:3.76|P-value:8.33E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL49(YJL096W)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL7(YDR237W)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MRPS35(YGR165W)|FD-Score:5.55|P-value:1.42E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MRPS5(YBR251W)|FD-Score:4.64|P-value:1.78E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSE1(YOL033W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:MST1(YKL194C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:PET112(YBL080C)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PET130(YJL023C)|FD-Score:5.41|P-value:3.12E-8||SGD DESC:Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PEX22(YAL055W)|FD-Score:-3.09|P-value:9.87E-4||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:QCR7(YDR529C)|FD-Score:3.83|P-value:6.49E-5||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RMD1(YDL001W)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Cytoplasmic protein required for sporulation Gene:RML2(YEL050C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RNH70(YGR276C)|FD-Score:5.65|P-value:7.94E-9||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:RNR3(YIL066C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Minor isoform of the large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits Gene:RPL13B(YMR142C)|FD-Score:7.42|P-value:5.83E-14||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS27B(YHR021C)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication Gene:RRG9(YNL213C)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RSM22(YKL155C)|FD-Score:5.85|P-value:2.48E-9||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RTC6(YPL183W-A)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SAC7(YDR389W)|FD-Score:-3.16|P-value:7.96E-4||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SED4(YCR067C)|FD-Score:5.19|P-value:1.05E-7||SGD DESC:Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p Gene:SKS1(YPL026C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Putative serine/threonine protein kinase; involved in the adaptation to low concentrations of glucose independent of the SNF3 regulated pathway Gene:SWM1(YDR260C)|FD-Score:4.66|P-value:1.62E-6||SGD DESC:Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation Gene:SWS2(YNL081C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TAT1(YBR069C)|FD-Score:-3.83|P-value:6.30E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA5(YLR426W_p)|FD-Score:-4.87|P-value:5.64E-7||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TOS6(YNL300W_p)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TRM3(YDL112W)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:TRP4(YDR354W)|FD-Score:-3.75|P-value:8.98E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:15.5|P-value:1.43E-54||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URE2(YNL229C)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VPS24(YKL041W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS62(YGR141W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins Gene:VPS8(YAL002W)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:YAL066W(YAL066W_d)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR116C(YBR116C_d)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TKL2 Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-4.48|P-value:3.81E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR102C(YDR102C_d)|FD-Score:4.39|P-value:5.65E-6||SGD DESC:Dubious open reading frame; homozygous diploid deletion strain exhibits high budding index Gene:YDR290W(YDR290W_d)|FD-Score:-3.37|P-value:3.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YEL010W(YEL010W_d)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER039C-A(YER039C-A_p)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Putative protein of unknown function; YER039C-A is not an essential gene Gene:YJR037W(YJR037W_d)|FD-Score:3.94|P-value:4.13E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces Gene:YKL102C(YKL102C_d)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site Gene:YLR118C(YLR118C)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YMD8(YML038C)|FD-Score:3.82|P-value:6.65E-5||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YML100W-A(YML100W-A_p)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YTA12(YMR089C)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes Gene:YTA7(YGR270W)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:ZRC1(YMR243C)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YOR207C15.006.73E-516.38RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YHR062C8.584.81E-180.47RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YBR002C8.112.49E-162.40RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YDL152W_d5.715.65E-90.30YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YDL150W5.413.11E-80.54RPC53RNA polymerase III subunit C53
YOR224C4.875.63E-70.50RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YGL097W4.376.27E-60.03SRM1Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p
YDR526C_d4.347.25E-60.44YDR526C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YPR103W3.904.83E-50.37PRE2Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
YDL193W3.532.11E-40.13NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YJL091C3.403.39E-40.30GWT1Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors
YGR082W3.099.88E-40.07TOM20Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins
YLR317W_d3.020.001250.02YLR317W_dDubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C
YCR057C3.000.001350.03PWP2Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis
YNL114C_d2.970.001500.04YNL114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A15.501.43E-54TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YMR142C7.425.83E-14RPL13BRibosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication
YMR138W6.651.48E-11CIN4GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog
YKL155C5.852.48E-9RSM22Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase
YGR276C5.657.94E-9RNH703'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts
YGR165W5.551.42E-8MRPS35Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets
YFR036W5.482.11E-8CDC26Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YJL023C5.413.12E-8PET130Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YPR060C5.191.05E-7ARO7Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis
YCR067C5.191.05E-7SED4Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p
YDR260C4.661.62E-6SWM1Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
YBR251W4.641.78E-6MRPS5Mitochondrial ribosomal protein of the small subunit
YDL202W4.621.95E-6MRPL11Mitochondrial ribosomal protein of the large subunit
YBR180W4.493.58E-6DTR1Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation
YFL023W4.434.65E-6BUD27Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP

GO enrichment analysis for SGTC_503
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3578.46E-177SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.104167TSC3-RPN4
0.3215.98E-142SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.0625
0.3103.52E-132SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.0606061TSC3-RPN4
0.3052.12E-127SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.145833TSC3-RPN4
0.2912.23E-115SGTC_1643st009866 20.2 μMTimTec (Natural product derivative library)31562730.0952381
0.2871.60E-112SGTC_23236148992 148.3 μMChembridge (Fragment library)28882200.0740741
0.2848.01E-110SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.102041
0.2761.17E-102SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651800.0757576
0.2711.39E-99SGTC_21135491891 107.0 μMChembridge (Fragment library)7899790.133333TSC3-RPN4
0.2707.35E-99SGTC_32829108498 15.5 μMChembridge (Drug-like library)164596450.0615385
0.2671.48E-96SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0740741TSC3-RPN4
0.2631.14E-93SGTC_21125488546 200.0 μMChembridge (Fragment library)6007570.0909091TSC3-RPN4
0.2626.41E-93SGTC_28pentachloropyridine N-oxide 1.6 μMChemDiv (Drug-like library)1402500.0294118TSC3-RPN4
0.2579.32E-90SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.05TSC3-RPN4
0.2571.91E-89SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.1TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_15852',4'-dihydroxychalcone10.4 μM0.3076925357218TimTec (Pure natural product library)240.253983.21823
SGTC_25732',4'-dihydroxychalcone15.04 μM0.3076925357218TimTec (Pure natural product library)240.253983.21823
SGTC_25742',4'-dihydroxychalcone14.64 μM0.3076925357218TimTec (Pure natural product library)240.253983.21823
SGTC_26602',4'-dihydroxychalcone17.14 μM0.3076925357218TimTec (Pure natural product library)240.253983.21823
SGTC_26682',4'-dihydroxychalcone15.08 μM0.3076925357218TimTec (Pure natural product library)240.253983.21823
SGTC_2565isoliquiritigenin100 μM0.307692638278Microsource (Natural product library)256.253382.97534
SGTC_1087pterostilbene9.05 μM0.3055565281727NIH Clinical Collection256.296443.54113
SGTC_2366907044160.58 μM0.2972976837235Chembridge (Fragment library)197.232541.09912
SGTC_26044'-hydroxychalcone11.32 μM0.2894745282362Microsource (Natural product library)224.254583.4612
SGTC_510ag-490170 μM0.2857145328779ICCB bioactive library294.304662.44434
SGTC_2129girgensonine121.05 μM0.282051442638Chembridge (Fragment library)216.278942.44413heme biosynthesis & mitochondrial translocase
SGTC_11544088-0362150 μM0.2765966618304ChemDiv (Drug-like library)282.360164.17714
SGTC_462ag-879158 μM0.2727276809654ICCB bioactive library316.460963.88224
SGTC_1651st01192886.1 μM0.266667720198TimTec (Natural product derivative library)232.258421.77414RSC complex & mRNA processing