nimesulide FDA approved compound

N-(4-nitro-2-phenoxyphenyl)methanesulfonamide

A COX2 inhibitor.

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PubChem MeSH terms: Anti-Inflammatory Agents, Non-Steroidal;Cyclooxygenase Inhibitors



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_507
Screen concentration 162.0 μM
Source Miscellaneous
PubChem CID 4495
SMILES CS(=O)(=O)NC1=C(C=C(C=C1)[N+](=O)[O-])OC2=CC=CC=C2
Standardized SMILES CS(=O)(=O)Nc1ccc(cc1Oc2ccccc2)[N+](=O)[O-]
Molecular weight 308.3098
ALogP 2.2
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.04
% growth inhibition (Hom. pool) 1.41


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4495
Download HIP data (tab-delimited text)  (excel)
Gene:ABF1(YKL112W)|FD-Score:-3.62|P-value:1.47E-4|Clearance:0||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:ACS2(YLR153C)|FD-Score:-4.11|P-value:1.98E-5|Clearance:0||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:CDC123(YLR215C)|FD-Score:3.17|P-value:7.60E-4|Clearance:0.06||SGD DESC:Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein Gene:DFR1(YOR236W)|FD-Score:3.44|P-value:2.87E-4|Clearance:0.02||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:IMP3(YHR148W)|FD-Score:4.38|P-value:6.01E-6|Clearance:0.77||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:NOP14(YDL148C)|FD-Score:3.2|P-value:6.94E-4|Clearance:0.03||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:PMI40(YER003C)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.07||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PRP28(YDR243C)|FD-Score:3.31|P-value:4.75E-4|Clearance:0.04||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:PWP1(YLR196W)|FD-Score:3.37|P-value:3.70E-4|Clearance:0.07||SGD DESC:Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily Gene:RPP1(YHR062C)|FD-Score:3.42|P-value:3.08E-4|Clearance:0.05||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP46(YGR095C)|FD-Score:-3.25|P-value:5.85E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SAS10(YDL153C)|FD-Score:3.45|P-value:2.80E-4|Clearance:0.01||SGD DESC:Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance Gene:SPC24(YMR117C)|FD-Score:3.6|P-value:1.57E-4|Clearance:0.15||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:TOM40(YMR203W)|FD-Score:-3.22|P-value:6.40E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:VAS1(YGR094W)|FD-Score:-3.89|P-value:4.92E-5|Clearance:0||SGD DESC:Mitochondrial and cytoplasmic valyl-tRNA synthetase Gene:YGR265W(YGR265W_d)|FD-Score:3.11|P-value:9.28E-4|Clearance:0.18||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:ABF1(YKL112W)|FD-Score:-3.62|P-value:1.47E-4|Clearance:0||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:ACS2(YLR153C)|FD-Score:-4.11|P-value:1.98E-5|Clearance:0||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:CDC123(YLR215C)|FD-Score:3.17|P-value:7.60E-4|Clearance:0.06||SGD DESC:Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein Gene:DFR1(YOR236W)|FD-Score:3.44|P-value:2.87E-4|Clearance:0.02||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:IMP3(YHR148W)|FD-Score:4.38|P-value:6.01E-6|Clearance:0.77||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:NOP14(YDL148C)|FD-Score:3.2|P-value:6.94E-4|Clearance:0.03||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:PMI40(YER003C)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.07||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PRP28(YDR243C)|FD-Score:3.31|P-value:4.75E-4|Clearance:0.04||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:PWP1(YLR196W)|FD-Score:3.37|P-value:3.70E-4|Clearance:0.07||SGD DESC:Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily Gene:RPP1(YHR062C)|FD-Score:3.42|P-value:3.08E-4|Clearance:0.05||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP46(YGR095C)|FD-Score:-3.25|P-value:5.85E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SAS10(YDL153C)|FD-Score:3.45|P-value:2.80E-4|Clearance:0.01||SGD DESC:Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance Gene:SPC24(YMR117C)|FD-Score:3.6|P-value:1.57E-4|Clearance:0.15||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:TOM40(YMR203W)|FD-Score:-3.22|P-value:6.40E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:VAS1(YGR094W)|FD-Score:-3.89|P-value:4.92E-5|Clearance:0||SGD DESC:Mitochondrial and cytoplasmic valyl-tRNA synthetase Gene:YGR265W(YGR265W_d)|FD-Score:3.11|P-value:9.28E-4|Clearance:0.18||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4495
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:-3.16|P-value:7.76E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AFT1(YGL071W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM37(YNL100W)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:AIR1(YIL079C)|FD-Score:-3.12|P-value:8.94E-4||SGD DESC:Zinc knuckle protein; involved in nuclear RNA processing and degradation as a component of the TRAMP complex; stimulates the poly(A) polymerase activity of Pap2p in vitro; AIR1 has a paralog, AIR2, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:3.75|P-value:8.87E-5||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATP22(YDR350C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Specific translational activator for the mitochondrial ATP6 mRNA, encoding a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane Gene:CCS1(YMR038C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CNM67(YNL225C)|FD-Score:-3.89|P-value:5.01E-5||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COG8(YML071C)|FD-Score:7.53|P-value:2.53E-14||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX23(YHR116W)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs Gene:CSG2(YBR036C)|FD-Score:-3.92|P-value:4.43E-5||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CSM3(YMR048W)|FD-Score:-4.35|P-value:6.66E-6||SGD DESC:Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress Gene:EXG2(YDR261C)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:GAC1(YOR178C)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock Gene:GCN2(YDR283C)|FD-Score:4.95|P-value:3.76E-7||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GTT2(YLL060C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:GUP1(YGL084C)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:IMO32(YGR031W)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 Gene:IRS4(YKR019C)|FD-Score:7.29|P-value:1.60E-13||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KEL3(YPL263C)|FD-Score:5.58|P-value:1.23E-8||SGD DESC:Cytoplasmic protein of unknown function Gene:MDV1(YJL112W)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication Gene:MNN2(YBR015C)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRP13(YGR084C)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NIT2(YJL126W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NRM1(YNR009W)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:OPY2(YPR075C)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:PAD1(YDR538W)|FD-Score:-3.82|P-value:6.60E-5||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PDR1(YGL013C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PFK1(YGR240C)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PIH1(YHR034C)|FD-Score:-3.76|P-value:8.62E-5||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PMP3(YDR276C)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PPZ1(YML016C)|FD-Score:-3.99|P-value:3.34E-5||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:PTC6(YCR079W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:QDR3(YBR043C)|FD-Score:3.76|P-value:8.65E-5||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:REC104(YHR157W)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RPL13B(YMR142C)|FD-Score:-3.28|P-value:5.16E-4||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RSM28(YDR494W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:RUP1(YOR138C)|FD-Score:4.91|P-value:4.45E-7||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SHY1(YGR112W)|FD-Score:3.92|P-value:4.49E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome Gene:SLT2(YHR030C)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SRN2(YLR119W)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:TIP1(YBR067C)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins Gene:TRP1(YDR007W)|FD-Score:4.9|P-value:4.86E-7||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:8|P-value:6.22E-16||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:7.07|P-value:7.58E-13||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:10.4|P-value:1.61E-25||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBX4(YMR067C)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:VFA1(YER128W)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VMR1(YHL035C)|FD-Score:3.73|P-value:9.61E-5||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VPS55(YJR044C)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YAL065C(YAL065C_p)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YAL066W(YAL066W_d)|FD-Score:3.77|P-value:8.13E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR053C(YBR053C_p)|FD-Score:-3.87|P-value:5.53E-5||SGD DESC:Putative protein of unknown function; induced by cell wall perturbation Gene:YBR232C(YBR232C_d)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR100C(YCR100C_p)|FD-Score:-3.3|P-value:4.81E-4||SGD DESC:Putative protein of unknown function Gene:YDR008C(YDR008C_d)|FD-Score:7.23|P-value:2.35E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL072C(YGL072C_d)|FD-Score:-3.23|P-value:6.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL117W(YGL117W_p)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Putative protein of unknown function Gene:YIA6(YIL006W)|FD-Score:-3.36|P-value:3.93E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YIL163C(YIL163C_p)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YLR253W(YLR253W_p)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; transcription is periodic during the metabolic cycle; YLR253W is not an essential gene Gene:YNR071C(YNR071C_p)|FD-Score:5.82|P-value:2.99E-9||SGD DESC:Putative aldose 1-epimerase Gene:YPR123C(YPR123C_d)|FD-Score:4.56|P-value:2.55E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR147C(YPR147C_p)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:ACB1(YGR037C)|FD-Score:-3.16|P-value:7.76E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AFT1(YGL071W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM37(YNL100W)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:AIR1(YIL079C)|FD-Score:-3.12|P-value:8.94E-4||SGD DESC:Zinc knuckle protein; involved in nuclear RNA processing and degradation as a component of the TRAMP complex; stimulates the poly(A) polymerase activity of Pap2p in vitro; AIR1 has a paralog, AIR2, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:3.75|P-value:8.87E-5||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATP22(YDR350C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Specific translational activator for the mitochondrial ATP6 mRNA, encoding a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane Gene:CCS1(YMR038C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CNM67(YNL225C)|FD-Score:-3.89|P-value:5.01E-5||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COG8(YML071C)|FD-Score:7.53|P-value:2.53E-14||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX23(YHR116W)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs Gene:CSG2(YBR036C)|FD-Score:-3.92|P-value:4.43E-5||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CSM3(YMR048W)|FD-Score:-4.35|P-value:6.66E-6||SGD DESC:Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress Gene:EXG2(YDR261C)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:GAC1(YOR178C)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock Gene:GCN2(YDR283C)|FD-Score:4.95|P-value:3.76E-7||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GTT2(YLL060C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:GUP1(YGL084C)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:IMO32(YGR031W)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 Gene:IRS4(YKR019C)|FD-Score:7.29|P-value:1.60E-13||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KEL3(YPL263C)|FD-Score:5.58|P-value:1.23E-8||SGD DESC:Cytoplasmic protein of unknown function Gene:MDV1(YJL112W)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication Gene:MNN2(YBR015C)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRP13(YGR084C)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NIT2(YJL126W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NRM1(YNR009W)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:OPY2(YPR075C)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:PAD1(YDR538W)|FD-Score:-3.82|P-value:6.60E-5||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PDR1(YGL013C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PFK1(YGR240C)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PIH1(YHR034C)|FD-Score:-3.76|P-value:8.62E-5||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PMP3(YDR276C)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PPZ1(YML016C)|FD-Score:-3.99|P-value:3.34E-5||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:PTC6(YCR079W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:QDR3(YBR043C)|FD-Score:3.76|P-value:8.65E-5||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:REC104(YHR157W)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RPL13B(YMR142C)|FD-Score:-3.28|P-value:5.16E-4||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RSM28(YDR494W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:RUP1(YOR138C)|FD-Score:4.91|P-value:4.45E-7||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SHY1(YGR112W)|FD-Score:3.92|P-value:4.49E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome Gene:SLT2(YHR030C)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SRN2(YLR119W)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:TIP1(YBR067C)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins Gene:TRP1(YDR007W)|FD-Score:4.9|P-value:4.86E-7||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:8|P-value:6.22E-16||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:7.07|P-value:7.58E-13||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:10.4|P-value:1.61E-25||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBX4(YMR067C)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:VFA1(YER128W)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VMR1(YHL035C)|FD-Score:3.73|P-value:9.61E-5||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VPS55(YJR044C)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YAL065C(YAL065C_p)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YAL066W(YAL066W_d)|FD-Score:3.77|P-value:8.13E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR053C(YBR053C_p)|FD-Score:-3.87|P-value:5.53E-5||SGD DESC:Putative protein of unknown function; induced by cell wall perturbation Gene:YBR232C(YBR232C_d)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR100C(YCR100C_p)|FD-Score:-3.3|P-value:4.81E-4||SGD DESC:Putative protein of unknown function Gene:YDR008C(YDR008C_d)|FD-Score:7.23|P-value:2.35E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL072C(YGL072C_d)|FD-Score:-3.23|P-value:6.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL117W(YGL117W_p)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Putative protein of unknown function Gene:YIA6(YIL006W)|FD-Score:-3.36|P-value:3.93E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YIL163C(YIL163C_p)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YLR253W(YLR253W_p)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; transcription is periodic during the metabolic cycle; YLR253W is not an essential gene Gene:YNR071C(YNR071C_p)|FD-Score:5.82|P-value:2.99E-9||SGD DESC:Putative aldose 1-epimerase Gene:YPR123C(YPR123C_d)|FD-Score:4.56|P-value:2.55E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR147C(YPR147C_p)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR148W4.386.01E-60.77IMP3Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA
YMR117C3.601.57E-40.15SPC24Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
YDL153C3.452.80E-40.01SAS10Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance
YOR236W3.442.87E-40.02DFR1Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
YHR062C3.423.08E-40.05RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YLR196W3.373.70E-40.07PWP1Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily
YDR243C3.314.75E-40.04PRP28RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site
YER003C3.275.42E-40.07PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YDL148C3.206.94E-40.03NOP14Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
YLR215C3.177.60E-40.06CDC123Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein
YGR265W_d3.119.28E-40.18YGR265W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase
YJL050W2.930.001700.03MTR4ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
YLR195C2.900.001870.07NMT1N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
YGR116W2.830.002360.07SPT6Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region
YOR278W2.750.002950.11HEM4Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR354W10.401.61E-25TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YER090W8.006.22E-16TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YML071C7.532.53E-14COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YKR019C7.291.60E-13IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YDR008C_d7.232.35E-13YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YKL211C7.077.58E-13TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YNR071C_p5.822.99E-9YNR071C_pPutative aldose 1-epimerase
YPL263C5.581.23E-8KEL3Cytoplasmic protein of unknown function
YDR283C4.953.76E-7GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YOR138C4.914.45E-7RUP1Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress
YDR007W4.904.86E-7TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YDR127W4.572.42E-6ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YPR123C_d4.562.55E-6YPR123C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR
YPR075C4.111.95E-5OPY2Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress
YDR276C4.022.88E-5PMP3Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential

GO enrichment analysis for SGTC_507
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3582.47E-178SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0555556
0.3403.58E-159SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0224719
0.3365.87E-156SGTC_24885268135 45.7 μMMiscellaneous12703560.111111
0.3321.28E-151SGTC_18815652484 16.0 μMMiscellaneous22551290.115385TRP & mitochondrial translation
0.3318.07E-151SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.106061
0.3279.67E-147SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0555556
0.3262.67E-146SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0864198
0.3231.33E-143SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0410959
0.3211.58E-141SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0344828
0.3175.16E-138SGTC_18825615643 20.0 μMMiscellaneous22530750.0864198TRP & mitochondrial translation
0.3178.14E-138SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.118644
0.3168.79E-137SGTC_505gw-5074 63.9 μMICCB bioactive library59242080.0704225
0.3158.16E-136SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.0967742
0.3135.37E-134SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.0972222
0.3102.17E-131SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.0735294

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_8890919-152482.7 μM0.4107143905452ChemDiv (Drug-like library)274.271962.85825
SGTC_410478-063282.64 μM0.4081633750424ChemDiv (Drug-like library)229.234642.52724
SGTC_7680384-003377.3 μM0.43965305ChemDiv (Drug-like library)275.216962.43216fatty acid desaturase (OLE1)
SGTC_2820798963071.43 μM0.42984098Chembridge (Drug-like library)416.877983.7525ERG2
SGTC_480382-001855 μM0.387755762596ChemDiv (Drug-like library)231.204163.04214
SGTC_260302-0163425.16 μM0.3653853100915ChemDiv (Drug-like library)275.216962.92626heme biosynthesis & mitochondrial translocase
SGTC_225nsc-1226571.02 μM0.358491275685Miscellaneous322.29332.72806
SGTC_9491165-0505146 μM0.3584916244267ChemDiv (Drug-like library)304.321082.15415
SGTC_15141189-1591158 μM0.345455767698ChemDiv (Drug-like library)320.363543.6715
SGTC_3710195-00067.36 μM0.3404263928593ChemDiv (Drug-like library)253.209682.70204