roscovitine

(2R)-2-[[6-(benzylamino)-9-propan-2-ylpurin-2-yl]amino]butan-1-ol

A CDKinase inhibitor.

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PubChem MeSH terms: Antineoplastic Agents;Protein Kinase Inhibitors



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_508
Screen concentration 141.0 μM
Source ICCB bioactive library
PubChem CID 160355
SMILES CCC(CO)NC1=NC2=C(C(=N1)NCC3=CC=CC=C3)N=CN2C(C)C
Standardized SMILES CCC(CO)Nc1nc(NCc2ccccc2)c3ncn(C(C)C)c3n1
Molecular weight 354.4493
ALogP 3.32
H-bond donor count 3
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) -0.02


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 160355
Download HIP data (tab-delimited text)  (excel)
Gene:ILV3(YJR016C)|FD-Score:-3.2|P-value:6.76E-4|Clearance:0||SGD DESC:Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids Gene:RFC2(YJR068W)|FD-Score:3.19|P-value:7.21E-4|Clearance:0.23||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:TEL2(YGR099W)|FD-Score:5.09|P-value:1.82E-7|Clearance:1.66||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TIP20(YGL145W)|FD-Score:3.42|P-value:3.08E-4|Clearance:0.24||SGD DESC:Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p Gene:ILV3(YJR016C)|FD-Score:-3.2|P-value:6.76E-4|Clearance:0||SGD DESC:Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids Gene:RFC2(YJR068W)|FD-Score:3.19|P-value:7.21E-4|Clearance:0.23||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:TEL2(YGR099W)|FD-Score:5.09|P-value:1.82E-7|Clearance:1.66||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TIP20(YGL145W)|FD-Score:3.42|P-value:3.08E-4|Clearance:0.24||SGD DESC:Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 160355
Download HOP data (tab-delimited text)  (excel)
Gene:AXL2(YIL140W)|FD-Score:3.8|P-value:7.38E-5||SGD DESC:Integral plasma membrane protein required for axial budding in haploid cells, localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate Gene:CBS2(YDR197W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CDC26(YFR036W)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:DPH5(YLR172C)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:GAL83(YER027C)|FD-Score:-4.22|P-value:1.20E-5||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:HEH2(YDR458C)|FD-Score:3.75|P-value:8.88E-5||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HST4(YDR191W)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism Gene:HTB2(YBL002W)|FD-Score:4.47|P-value:3.90E-6||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:IMO32(YGR031W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 Gene:ISF1(YMR081C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Serine-rich, hydrophilic protein with similarity to Mbr1p; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant Gene:LRO1(YNR008W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:MDM38(YOL027C)|FD-Score:-3.26|P-value:5.48E-4||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:NMA1(YLR328W)|FD-Score:3.89|P-value:4.93E-5||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication Gene:PCL5(YHR071W)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Cyclin, interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity Gene:REX2(YLR059C)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RNH70(YGR276C)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:RPS23A(YGR118W)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:SAW1(YAL027W)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:SGE1(YPR198W)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SHM2(YLR058C)|FD-Score:3.8|P-value:7.37E-5||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SNX4(YJL036W)|FD-Score:-4.6|P-value:2.16E-6||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:TOF2(YKR010C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication Gene:TPC1(YGR096W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family Gene:TRF5(YNL299W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:UFD4(YKL010C)|FD-Score:-4.19|P-value:1.37E-5||SGD DESC:Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR222W(YDR222W_p)|FD-Score:3.75|P-value:8.78E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YER137C(YER137C_p)|FD-Score:-3.22|P-value:6.47E-4||SGD DESC:Putative protein of unknown function Gene:YJR154W(YJR154W_p)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKL018C-A(YKL018C-A_p)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Putative protein of unknown function; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR171W(YLR171W_d)|FD-Score:3.92|P-value:4.52E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR269C(YLR269C_d)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR074C(YMR074C)|FD-Score:-4.68|P-value:1.42E-6||SGD DESC:Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:AXL2(YIL140W)|FD-Score:3.8|P-value:7.38E-5||SGD DESC:Integral plasma membrane protein required for axial budding in haploid cells, localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate Gene:CBS2(YDR197W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CDC26(YFR036W)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:DPH5(YLR172C)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:GAL83(YER027C)|FD-Score:-4.22|P-value:1.20E-5||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:HEH2(YDR458C)|FD-Score:3.75|P-value:8.88E-5||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HST4(YDR191W)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism Gene:HTB2(YBL002W)|FD-Score:4.47|P-value:3.90E-6||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:IMO32(YGR031W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 Gene:ISF1(YMR081C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Serine-rich, hydrophilic protein with similarity to Mbr1p; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant Gene:LRO1(YNR008W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:MDM38(YOL027C)|FD-Score:-3.26|P-value:5.48E-4||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:NMA1(YLR328W)|FD-Score:3.89|P-value:4.93E-5||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication Gene:PCL5(YHR071W)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Cyclin, interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity Gene:REX2(YLR059C)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RNH70(YGR276C)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:RPS23A(YGR118W)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:SAW1(YAL027W)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:SGE1(YPR198W)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SHM2(YLR058C)|FD-Score:3.8|P-value:7.37E-5||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SNX4(YJL036W)|FD-Score:-4.6|P-value:2.16E-6||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:TOF2(YKR010C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication Gene:TPC1(YGR096W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family Gene:TRF5(YNL299W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:UFD4(YKL010C)|FD-Score:-4.19|P-value:1.37E-5||SGD DESC:Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR222W(YDR222W_p)|FD-Score:3.75|P-value:8.78E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YER137C(YER137C_p)|FD-Score:-3.22|P-value:6.47E-4||SGD DESC:Putative protein of unknown function Gene:YJR154W(YJR154W_p)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKL018C-A(YKL018C-A_p)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Putative protein of unknown function; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR171W(YLR171W_d)|FD-Score:3.92|P-value:4.52E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR269C(YLR269C_d)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR074C(YMR074C)|FD-Score:-4.68|P-value:1.42E-6||SGD DESC:Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR099W5.091.82E-71.66TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
YGL145W3.423.08E-40.24TIP20Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p
YJR068W3.197.21E-40.23RFC2Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YKL182W2.960.001550.05FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YKL125W2.900.001840.15RRN3Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
YDR060W2.750.002940.05MAK21Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
YGL102C_d2.710.003390.07YGL102C_dDubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein
YER003C2.630.004210.02PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YDL084W2.610.004490.02SUB2Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress
YPR165W2.600.004710.00RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YOR278W2.590.004760.00HEM4Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
YPL210C2.590.004790.03SRP72Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane
YGR030C2.560.005200.06POP6Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YNR017W2.500.006240.02TIM23Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel
YDR189W2.480.006520.00SLY1Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBL002W4.473.90E-6HTB2Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
YFR036W4.161.59E-5CDC26Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YHR071W4.141.73E-5PCL5Cyclin, interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity
YDR191W4.111.94E-5HST4Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism
YKL018C-A_p4.032.84E-5YKL018C-A_pPutative protein of unknown function; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YLR171W_d3.924.52E-5YLR171W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YLR328W3.894.93E-5NMA1Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication
YLR058C3.807.37E-5SHM2Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis
YIL140W3.807.38E-5AXL2Integral plasma membrane protein required for axial budding in haploid cells, localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate
YDR222W_p3.758.78E-5YDR222W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YDR458C3.758.88E-5HEH2Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle
YGR031W3.512.23E-4IMO32Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1
YNL299W3.452.79E-4TRF5Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p
YGR118W3.433.00E-4RPS23ARibosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal
YGR096W3.373.81E-4TPC1Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family

GO enrichment analysis for SGTC_508
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2419.06E-79SGTC_510ag-490 170.0 μMICCB bioactive library53287790.166667
0.1411.34E-27SGTC_499genistein 192.0 μMICCB bioactive library66103450.0512821
0.1241.56E-21SGTC_450kpi-0036 14.9 μMChemDiv (Drug-like library)27950210.0684932excess fatty acid
0.1055.28E-16SGTC_475α-linolenic acid 10.0 μMICCB bioactive library52809340.0547945excess fatty acid
0.1015.86E-15SGTC_509pifithrin 175.0 μMICCB bioactive library48170.0595238mitochondrial processes
0.0912.47E-12SGTC_15110906-3109 28.5 μMChemDiv (Drug-like library)28821800.0722892excess fatty acid
0.0756.75E-9SGTC_9473447-0010 211.0 μMChemDiv (Drug-like library)7483500.103896
0.0723.40E-8SGTC_12280124-0032 52.5 μMChemDiv (Drug-like library)X12280.0757576
0.0714.45E-8SGTC_820054-0107 293.8 μMChemDiv (Drug-like library)31046970.102564
0.0707.70E-8SGTC_410α-linolenic acid 100.0 μMMiscellaneous8600.0547945excess fatty acid
0.0681.77E-7SGTC_30229087993 49.5 μMChembridge (Drug-like library)171736440.0786517
0.0672.57E-7SGTC_14314049-0260 9.1 μMChemDiv (Drug-like library)38548410.0843373
0.0664.30E-7SGTC_24275545055 42.8 μMMiscellaneous53728580.150685
0.0622.15E-6SGTC_33029125395 16.4 μMChembridge (Drug-like library)175996680.155844
0.0594.89E-6SGTC_14770139-0234 16.0 μMChemDiv (Drug-like library)6208800.0821918

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_506n9-isopropylolomoucine153 μM0.7142866610355ICCB bioactive library326.396182.41936
SGTC_1539benzyladenosine56 μM0.35616492208TimTec (Pure natural product library)357.363860.25848
SGTC_14023909-767335.9 μM0.2388065328014ChemDiv (Drug-like library)269.728883.98313
SGTC_3321913779326.83 μM0.2345684904192Chembridge (Drug-like library)343.424943.63414cell wall
SGTC_332291390582.11 μM0.2345684908926Chembridge (Drug-like library)343.424943.80714cell wall
SGTC_3351915031221.6 μM0.2307694904905Chembridge (Drug-like library)329.398363.17114cell wall
SGTC_335691518114.48 μM0.2289161859254Chembridge (Drug-like library)357.451524.0914cell wall
SGTC_23729071633200 μM0.22580621321366Chembridge (Fragment library)199.248422.9822
SGTC_331191311462.88 μM0.2254900406Chembridge (Drug-like library)343.424944.02914cell wall
SGTC_11453546-059710.2 μM0.216216875334ChemDiv (Drug-like library)293.406024.39812mitochondrial processes