pifithrin

2-(2-imino-4,5,6,7-tetrahydro-1,3-benzothiazol-3-yl)-1-(4-methylphenyl)ethanone

A p53 inhibitor.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_509
Screen concentration 175.0 μM
Source ICCB bioactive library
PubChem CID 4817
SMILES CC1=CC=C(C=C1)C(=O)CN2C3=C(CCCC3)SC2=N
Standardized SMILES Cc1ccc(cc1)C(=O)CN2C(=N)SC3=C2CCCC3
Molecular weight 286.3919
ALogP 3.5
H-bond donor count 1
H-bond acceptor count 3
Response signature mitochondrial processes

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.1
% growth inhibition (Hom. pool) 1.41


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4817
Download HIP data (tab-delimited text)  (excel)
Gene:CDC31(YOR257W)|FD-Score:3.6|P-value:1.57E-4|Clearance:0.27||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:RSC58(YLR033W)|FD-Score:4.39|P-value:5.58E-6|Clearance:0.66||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:3.73|P-value:9.52E-5|Clearance:0.09||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:4.97|P-value:3.37E-7|Clearance:0.66||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:TFC3(YAL001C)|FD-Score:3.34|P-value:4.24E-4|Clearance:0.27||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:YDL196W(YDL196W_d)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:CDC31(YOR257W)|FD-Score:3.6|P-value:1.57E-4|Clearance:0.27||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:RSC58(YLR033W)|FD-Score:4.39|P-value:5.58E-6|Clearance:0.66||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:3.73|P-value:9.52E-5|Clearance:0.09||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:4.97|P-value:3.37E-7|Clearance:0.66||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:TFC3(YAL001C)|FD-Score:3.34|P-value:4.24E-4|Clearance:0.27||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:YDL196W(YDL196W_d)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4817
Download HOP data (tab-delimited text)  (excel)
Gene:AHA1(YDR214W)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:APL3(YBL037W)|FD-Score:-3.36|P-value:3.93E-4||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:ARO10(YDR380W)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Phenylpyruvate decarboxylase, catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway Gene:ARO7(YPR060C)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ASK10(YGR097W)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Component of RNA polymerase II holoenzyme; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; proposed to function in activation of the glycerol channel Fps1p; ASK10 has a paralog, RGC1, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:3.99|P-value:3.27E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:ECM18(YDR125C)|FD-Score:-3.36|P-value:3.96E-4||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:FMP33(YJL161W_p)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NEJ1(YLR265C)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:NMA1(YLR328W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication Gene:OYE3(YPL171C)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death Gene:RIB4(YOL143C)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin Gene:SCM4(YGR049W)|FD-Score:4.71|P-value:1.21E-6||SGD DESC:Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation Gene:SLT2(YHR030C)|FD-Score:6.57|P-value:2.52E-11||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SOL4(YGR248W)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWP82(YFL049W)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions Gene:TDA5(YLR426W_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TDH1(YJL052W)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:UBA4(YHR111W)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:YDR132C(YDR132C_p)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Protein of unknown function; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; YDR132C has a paralog, YLR108C, that arose from the whole genome duplication Gene:YDR182W-A(YDR182W-A_p)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YFL042C(YFL042C_p)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YGR242W(YGR242W_d)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YJR015W(YJR015W_p)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene Gene:YKL018C-A(YKL018C-A_p)|FD-Score:4.32|P-value:7.74E-6||SGD DESC:Putative protein of unknown function; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKL115C(YKL115C_d)|FD-Score:3.13|P-value:8.59E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YMR074C(YMR074C)|FD-Score:-4.32|P-value:7.89E-6||SGD DESC:Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR1(YJR110W)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family Gene:YPT6(YLR262C)|FD-Score:-3.46|P-value:2.65E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:AHA1(YDR214W)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:APL3(YBL037W)|FD-Score:-3.36|P-value:3.93E-4||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:ARO10(YDR380W)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Phenylpyruvate decarboxylase, catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway Gene:ARO7(YPR060C)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ASK10(YGR097W)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Component of RNA polymerase II holoenzyme; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; proposed to function in activation of the glycerol channel Fps1p; ASK10 has a paralog, RGC1, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:3.99|P-value:3.27E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:ECM18(YDR125C)|FD-Score:-3.36|P-value:3.96E-4||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:FMP33(YJL161W_p)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NEJ1(YLR265C)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:NMA1(YLR328W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication Gene:OYE3(YPL171C)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death Gene:RIB4(YOL143C)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin Gene:SCM4(YGR049W)|FD-Score:4.71|P-value:1.21E-6||SGD DESC:Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation Gene:SLT2(YHR030C)|FD-Score:6.57|P-value:2.52E-11||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SOL4(YGR248W)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWP82(YFL049W)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions Gene:TDA5(YLR426W_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TDH1(YJL052W)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:UBA4(YHR111W)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:YDR132C(YDR132C_p)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Protein of unknown function; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; YDR132C has a paralog, YLR108C, that arose from the whole genome duplication Gene:YDR182W-A(YDR182W-A_p)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YFL042C(YFL042C_p)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YGR242W(YGR242W_d)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YJR015W(YJR015W_p)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene Gene:YKL018C-A(YKL018C-A_p)|FD-Score:4.32|P-value:7.74E-6||SGD DESC:Putative protein of unknown function; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKL115C(YKL115C_d)|FD-Score:3.13|P-value:8.59E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YMR074C(YMR074C)|FD-Score:-4.32|P-value:7.89E-6||SGD DESC:Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR1(YJR110W)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family Gene:YPT6(YLR262C)|FD-Score:-3.46|P-value:2.65E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YML127W4.973.37E-70.66RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YLR033W4.395.58E-60.66RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YFR037C3.739.52E-50.09RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YDL196W_d3.641.35E-40.04YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YOR257W3.601.57E-40.27CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YAL001C3.344.24E-40.27TFC3Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding
YHR197W3.070.001080.09RIX1Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene
YGR099W2.980.001430.04TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
YGR191W2.940.001660.03HIP1High-affinity histidine permease, also involved in the transport of manganese ions
YIL144W2.900.001850.05TID3Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering
YLR321C2.860.002140.05SFH1Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog
YPR107C2.810.002480.11YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YDR280W2.700.003440.01RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YJR042W2.700.003510.06NUP85Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75
YNR016C2.640.004190.04ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR030C6.572.52E-11SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YGR049W4.711.21E-6SCM4Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation
YKL018C-A_p4.327.74E-6YKL018C-A_pPutative protein of unknown function; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YDR380W4.121.87E-5ARO10Phenylpyruvate decarboxylase, catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway
YPL069C3.993.27E-5BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YGR242W_d3.944.05E-5YGR242W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C
YLR426W_p3.691.14E-4TDA5_pPutative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele
YLR328W3.611.50E-4NMA1Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication
YJL161W_p3.551.93E-4FMP33_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YDR132C_p3.512.24E-4YDR132C_pProtein of unknown function; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; YDR132C has a paralog, YLR108C, that arose from the whole genome duplication
YJL052W3.334.32E-4TDH1Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
YGR248W3.334.39E-4SOL46-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication
YDR293C3.314.59E-4SSD1Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
YPR060C3.295.05E-4ARO7Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis
YDR214W3.265.58E-4AHA1Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_509
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2243.99E-68SGTC_643k048-0106 28.4 μMChemDiv (Drug-like library)67639200.0533333RSC & ERG11
0.2048.47E-57SGTC_2534helenine 20.1 μMMicrosource (Natural product library)727240.0833333RSC complex & mRNA processing
0.1957.45E-52SGTC_29127964298 10.9 μMChembridge (Drug-like library)29758500.115942cell wall
0.1945.47E-51SGTC_7093807-1509 243.0 μMChemDiv (Drug-like library)18866770.0833333RSC complex & mRNA processing
0.1882.77E-48SGTC_483fpl-64176 144.0 μMICCB bioactive library34230.130435cell wall
0.1811.51E-44SGTC_21866124449 200.0 μMChembridge (Fragment library)38341820.0684932RSC complex & mRNA processing
0.1778.56E-43SGTC_28909046729 58.4 μMChembridge (Drug-like library)9033290.101449Golgi
0.1771.12E-42SGTC_1651st011928 86.1 μMTimTec (Natural product derivative library)7201980.0724638RSC complex & mRNA processing
0.1668.24E-38SGTC_1652st011932 78.0 μMTimTec (Natural product derivative library)15510760.126761RSC complex & mRNA processing
0.1652.60E-37SGTC_22297354935 190.0 μMChembridge (Fragment library)3181120.0606061RSC complex & mRNA processing
0.1604.02E-35SGTC_23789074213 121.4 μMChembridge (Fragment library)6577140.101695RSC complex & mRNA processing
0.1582.94E-34SGTC_3241315-0088 19.6 μMChemDiv (Drug-like library)67874210.0649351RSC & ERG11
0.1578.52E-34SGTC_237nikkomycin 200.0 μMMiscellaneous3534810.0808081RSC complex & mRNA processing
0.1555.09E-33SGTC_32779138724 49.5 μMChembridge (Drug-like library)159434180.0769231Golgi
0.1532.60E-32SGTC_2776terbinafine 19.9 μMNIH Clinical Collection52824810.0684932cell wall signaling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_15081080-062572.9 μM0.345455767111ChemDiv (Drug-like library)246.328062.25513
SGTC_12420402-010360.1 μM0.333333432868ChemDiv (Drug-like library)191.269482.3102
SGTC_12890976-007369.1 μM0.3076923391136ChemDiv (Drug-like library)253.29582.7812
SGTC_3242913394049.47 μM0.28787917248444Chembridge (Drug-like library)305.395261.94414
SGTC_24825981709142.65 μM0.258621877755Miscellaneous333.176624.14713PDR1
SGTC_1954st07703268.9 μM0.25698907TimTec (Natural product derivative library)290.359023.68611
SGTC_29087930301100 μM0.252223178Chembridge (Drug-like library)387.431143.00533
SGTC_12230764-0573147 μM0.2461541609797ChemDiv (Drug-like library)350.39113.54104
SGTC_2887904478458.44 μM0.2459026465546Chembridge (Drug-like library)323.4322.98313fatty acid desaturase (OLE1)
SGTC_3120912407749.47 μM0.24285718774327Chembridge (Drug-like library)326.389582.50703