1013-0126

S-[(5E)-5-benzylidene-4-oxo-1,3-thiazol-2-yl] 2-bromobenzenecarbothioate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_51
Screen concentration 23.4 μM
Source ChemDiv (Drug-like library)
PubChem CID 1627850
SMILES C1=CC=C(C=C1)C=C2C(=O)N=C(S2)SC(=O)C3=CC=CC=C3Br
Standardized SMILES Brc1ccccc1C(=O)SC2=NC(=O)C(=Cc3ccccc3)S2
Molecular weight 404.3008
ALogP 5.61
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 17.28
% growth inhibition (Hom. pool) 9.65


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1627850
Download HIP data (tab-delimited text)  (excel)
Gene:ABD1(YBR236C)|FD-Score:3.81|P-value:7.01E-5|Clearance:0.35||SGD DESC:Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA Gene:BMS1(YPL217C)|FD-Score:3.2|P-value:6.85E-4|Clearance:0||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CCT4(YDL143W)|FD-Score:-3.46|P-value:2.71E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:DPS1(YLL018C)|FD-Score:-5.12|P-value:1.52E-7|Clearance:0||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:DRE2(YKR071C)|FD-Score:3.22|P-value:6.33E-4|Clearance:0.02||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:GCD11(YER025W)|FD-Score:-3.33|P-value:4.36E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GCD6(YDR211W)|FD-Score:-3.62|P-value:1.47E-4|Clearance:0||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:ILV5(YLR355C)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.35||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:IRR1(YIL026C)|FD-Score:3.2|P-value:6.91E-4|Clearance:0.18||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:JIP5(YPR169W)|FD-Score:3.25|P-value:5.83E-4|Clearance:0.02||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:KRE33(YNL132W)|FD-Score:4.64|P-value:1.73E-6|Clearance:0.35||SGD DESC:Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:MCM1(YMR043W)|FD-Score:-3.62|P-value:1.45E-4|Clearance:0||SGD DESC:Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes Gene:NOP9(YJL010C)|FD-Score:-3.81|P-value:6.91E-5|Clearance:0||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:NUG1(YER006W)|FD-Score:-3.17|P-value:7.60E-4|Clearance:0||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:NUS1(YDL193W)|FD-Score:4.39|P-value:5.72E-6|Clearance:0.35||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:RIB2(YOL066C)|FD-Score:4.72|P-value:1.17E-6|Clearance:0.35||SGD DESC:Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs Gene:RPB10(YOR210W)|FD-Score:4.76|P-value:9.44E-7|Clearance:0.35||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPB8(YOR224C)|FD-Score:-3.6|P-value:1.62E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:SPC97(YHR172W)|FD-Score:-4.73|P-value:1.14E-6|Clearance:0||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TFC8(YPL007C)|FD-Score:-3.6|P-value:1.59E-4|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:THG1(YGR024C)|FD-Score:4.13|P-value:1.83E-5|Clearance:0.35||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:ABD1(YBR236C)|FD-Score:3.81|P-value:7.01E-5|Clearance:0.35||SGD DESC:Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA Gene:BMS1(YPL217C)|FD-Score:3.2|P-value:6.85E-4|Clearance:0||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CCT4(YDL143W)|FD-Score:-3.46|P-value:2.71E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:DPS1(YLL018C)|FD-Score:-5.12|P-value:1.52E-7|Clearance:0||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:DRE2(YKR071C)|FD-Score:3.22|P-value:6.33E-4|Clearance:0.02||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:GCD11(YER025W)|FD-Score:-3.33|P-value:4.36E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GCD6(YDR211W)|FD-Score:-3.62|P-value:1.47E-4|Clearance:0||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:ILV5(YLR355C)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.35||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:IRR1(YIL026C)|FD-Score:3.2|P-value:6.91E-4|Clearance:0.18||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:JIP5(YPR169W)|FD-Score:3.25|P-value:5.83E-4|Clearance:0.02||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:KRE33(YNL132W)|FD-Score:4.64|P-value:1.73E-6|Clearance:0.35||SGD DESC:Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:MCM1(YMR043W)|FD-Score:-3.62|P-value:1.45E-4|Clearance:0||SGD DESC:Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes Gene:NOP9(YJL010C)|FD-Score:-3.81|P-value:6.91E-5|Clearance:0||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:NUG1(YER006W)|FD-Score:-3.17|P-value:7.60E-4|Clearance:0||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:NUS1(YDL193W)|FD-Score:4.39|P-value:5.72E-6|Clearance:0.35||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:RIB2(YOL066C)|FD-Score:4.72|P-value:1.17E-6|Clearance:0.35||SGD DESC:Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs Gene:RPB10(YOR210W)|FD-Score:4.76|P-value:9.44E-7|Clearance:0.35||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPB8(YOR224C)|FD-Score:-3.6|P-value:1.62E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:SPC97(YHR172W)|FD-Score:-4.73|P-value:1.14E-6|Clearance:0||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TFC8(YPL007C)|FD-Score:-3.6|P-value:1.59E-4|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:THG1(YGR024C)|FD-Score:4.13|P-value:1.83E-5|Clearance:0.35||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1627850
Download HOP data (tab-delimited text)  (excel)
Gene:AHC2(YCR082W)|FD-Score:-4.41|P-value:5.15E-6||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:ARO1(YDR127W)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:7.7|P-value:6.79E-15||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ASF1(YJL115W)|FD-Score:-3.91|P-value:4.64E-5||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:ATP22(YDR350C)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Specific translational activator for the mitochondrial ATP6 mRNA, encoding a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane Gene:BUB3(YOR026W)|FD-Score:-3.1|P-value:9.83E-4||SGD DESC:Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p Gene:CLN2(YPL256C)|FD-Score:3.09|P-value:9.89E-4||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:CTF4(YPR135W)|FD-Score:5.82|P-value:2.99E-9||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:DAL7(YIR031C)|FD-Score:-4.15|P-value:1.63E-5||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DOA1(YKL213C)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DSE2(YHR143W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:ECM34(YHL043W)|FD-Score:5.87|P-value:2.18E-9||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:FMP43(YGR243W)|FD-Score:3.81|P-value:6.89E-5||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress Gene:FRA1(YLL029W)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:FSH3(YOR280C)|FD-Score:3.81|P-value:7.08E-5||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:GCN2(YDR283C)|FD-Score:9.32|P-value:5.68E-21||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:10.4|P-value:8.69E-26||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:6.64|P-value:1.60E-11||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:13.2|P-value:4.95E-40||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GDB1(YPR184W)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GDH1(YOR375C)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication Gene:GUT1(YHL032C)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HES1(YOR237W)|FD-Score:-3.15|P-value:8.25E-4||SGD DESC:Protein implicated in the regulation of ergosterol biosynthesis; one of a seven member gene family with a common essential function and non-essential unique functions; similar to human oxysterol binding protein (OSBP); HES1 has a paralog, KES1, that arose from the whole genome duplication Gene:HMO1(YDR174W)|FD-Score:-3.38|P-value:3.69E-4||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HSP31(YDR533C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:LAT1(YNL071W)|FD-Score:-3.91|P-value:4.61E-5||SGD DESC:Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA Gene:LHS1(YKL073W)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:LSB3(YFR024C-A)|FD-Score:-4.19|P-value:1.41E-5||SGD DESC:Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication Gene:MOG1(YJR074W)|FD-Score:-3.35|P-value:3.98E-4||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MSB4(YOL112W)|FD-Score:5.77|P-value:3.88E-9||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:MTD1(YKR080W)|FD-Score:5.39|P-value:3.61E-8||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:NPT1(YOR209C)|FD-Score:5.5|P-value:1.93E-8||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:OCH1(YGL038C)|FD-Score:-5.04|P-value:2.38E-7||SGD DESC:Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins Gene:PAA1(YDR071C)|FD-Score:6.57|P-value:2.56E-11||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PAU24(YBR301W)|FD-Score:4.46|P-value:4.11E-6||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PHO86(YJL117W)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PKR1(YMR123W)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PRM4(YPL156C)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift Gene:PSK2(YOL045W)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:PUS7(YOR243C)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:QCR6(YFR033C)|FD-Score:4.97|P-value:3.38E-7||SGD DESC:Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space Gene:RCK2(YLR248W)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RNH201(YNL072W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RPL12B(YDR418W)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RPL31A(YDL075W)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:SFB3(YHR098C)|FD-Score:12.4|P-value:7.33E-36||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SIP2(YGL208W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane Gene:SIS2(YKR072C)|FD-Score:-4.98|P-value:3.13E-7||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SLH1(YGR271W)|FD-Score:-4.73|P-value:1.10E-6||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SPS19(YNL202W)|FD-Score:-3.22|P-value:6.48E-4||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SUR4(YLR372W)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TGL4(YKR089C)|FD-Score:-3.26|P-value:5.48E-4||SGD DESC:Multifunctional triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p Gene:TIS11(YLR136C)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:TOR1(YJR066W)|FD-Score:4.77|P-value:9.16E-7||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication Gene:TOS6(YNL300W_p)|FD-Score:4.48|P-value:3.72E-6||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TRP1(YDR007W)|FD-Score:12.4|P-value:2.32E-35||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:12.5|P-value:3.53E-36||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:12|P-value:1.73E-33||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:19.9|P-value:9.92E-89||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:10.4|P-value:9.45E-26||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:UGA2(YBR006W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VMR1(YHL035C)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:YAP1801(YHR161C)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YBR138C(YBR138C_p)|FD-Score:4.5|P-value:3.40E-6||SGD DESC:Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene Gene:YCR101C(YCR101C_p)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:15.5|P-value:1.19E-54||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR262W(YDR262W_p)|FD-Score:4.33|P-value:7.53E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole and is induced in response to the DNA-damaging agent MMS; gene expression increases in response to Zymoliase treatment Gene:YER097W(YER097W_d)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFR032C-B(YFR032C-B_p)|FD-Score:-3.11|P-value:9.24E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGL117W(YGL117W_p)|FD-Score:4.07|P-value:2.32E-5||SGD DESC:Putative protein of unknown function Gene:YIL029C(YIL029C_p)|FD-Score:-3.89|P-value:4.94E-5||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YIR024C(YIR024C)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect Gene:YJL120W(YJL120W_d)|FD-Score:-3.14|P-value:8.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YNL144C(YNL144C_p)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study Gene:YOR331C(YOR331C_d)|FD-Score:-3.73|P-value:9.62E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W Gene:YPR012W(YPR012W_d)|FD-Score:-4|P-value:3.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:YPR096C(YPR096C)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YPR097W(YPR097W)|FD-Score:5.06|P-value:2.14E-7||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR117W(YPR117W_p)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Putative protein of unknown function Gene:AHC2(YCR082W)|FD-Score:-4.41|P-value:5.15E-6||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:ARO1(YDR127W)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:7.7|P-value:6.79E-15||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ASF1(YJL115W)|FD-Score:-3.91|P-value:4.64E-5||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:ATP22(YDR350C)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Specific translational activator for the mitochondrial ATP6 mRNA, encoding a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane Gene:BUB3(YOR026W)|FD-Score:-3.1|P-value:9.83E-4||SGD DESC:Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p Gene:CLN2(YPL256C)|FD-Score:3.09|P-value:9.89E-4||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:CTF4(YPR135W)|FD-Score:5.82|P-value:2.99E-9||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:DAL7(YIR031C)|FD-Score:-4.15|P-value:1.63E-5||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DOA1(YKL213C)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DSE2(YHR143W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:ECM34(YHL043W)|FD-Score:5.87|P-value:2.18E-9||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:FMP43(YGR243W)|FD-Score:3.81|P-value:6.89E-5||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress Gene:FRA1(YLL029W)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:FSH3(YOR280C)|FD-Score:3.81|P-value:7.08E-5||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:GCN2(YDR283C)|FD-Score:9.32|P-value:5.68E-21||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:10.4|P-value:8.69E-26||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:6.64|P-value:1.60E-11||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:13.2|P-value:4.95E-40||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GDB1(YPR184W)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GDH1(YOR375C)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication Gene:GUT1(YHL032C)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HES1(YOR237W)|FD-Score:-3.15|P-value:8.25E-4||SGD DESC:Protein implicated in the regulation of ergosterol biosynthesis; one of a seven member gene family with a common essential function and non-essential unique functions; similar to human oxysterol binding protein (OSBP); HES1 has a paralog, KES1, that arose from the whole genome duplication Gene:HMO1(YDR174W)|FD-Score:-3.38|P-value:3.69E-4||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HSP31(YDR533C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:LAT1(YNL071W)|FD-Score:-3.91|P-value:4.61E-5||SGD DESC:Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA Gene:LHS1(YKL073W)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:LSB3(YFR024C-A)|FD-Score:-4.19|P-value:1.41E-5||SGD DESC:Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication Gene:MOG1(YJR074W)|FD-Score:-3.35|P-value:3.98E-4||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MSB4(YOL112W)|FD-Score:5.77|P-value:3.88E-9||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:MTD1(YKR080W)|FD-Score:5.39|P-value:3.61E-8||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:NPT1(YOR209C)|FD-Score:5.5|P-value:1.93E-8||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:OCH1(YGL038C)|FD-Score:-5.04|P-value:2.38E-7||SGD DESC:Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins Gene:PAA1(YDR071C)|FD-Score:6.57|P-value:2.56E-11||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PAU24(YBR301W)|FD-Score:4.46|P-value:4.11E-6||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PHO86(YJL117W)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PKR1(YMR123W)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PRM4(YPL156C)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift Gene:PSK2(YOL045W)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:PUS7(YOR243C)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:QCR6(YFR033C)|FD-Score:4.97|P-value:3.38E-7||SGD DESC:Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space Gene:RCK2(YLR248W)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RNH201(YNL072W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RPL12B(YDR418W)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RPL31A(YDL075W)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:SFB3(YHR098C)|FD-Score:12.4|P-value:7.33E-36||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SIP2(YGL208W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane Gene:SIS2(YKR072C)|FD-Score:-4.98|P-value:3.13E-7||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SLH1(YGR271W)|FD-Score:-4.73|P-value:1.10E-6||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SPS19(YNL202W)|FD-Score:-3.22|P-value:6.48E-4||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SUR4(YLR372W)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TGL4(YKR089C)|FD-Score:-3.26|P-value:5.48E-4||SGD DESC:Multifunctional triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p Gene:TIS11(YLR136C)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:TOR1(YJR066W)|FD-Score:4.77|P-value:9.16E-7||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication Gene:TOS6(YNL300W_p)|FD-Score:4.48|P-value:3.72E-6||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TRP1(YDR007W)|FD-Score:12.4|P-value:2.32E-35||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:12.5|P-value:3.53E-36||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:12|P-value:1.73E-33||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:19.9|P-value:9.92E-89||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:10.4|P-value:9.45E-26||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:UGA2(YBR006W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VMR1(YHL035C)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:YAP1801(YHR161C)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YBR138C(YBR138C_p)|FD-Score:4.5|P-value:3.40E-6||SGD DESC:Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene Gene:YCR101C(YCR101C_p)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:15.5|P-value:1.19E-54||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR262W(YDR262W_p)|FD-Score:4.33|P-value:7.53E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole and is induced in response to the DNA-damaging agent MMS; gene expression increases in response to Zymoliase treatment Gene:YER097W(YER097W_d)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFR032C-B(YFR032C-B_p)|FD-Score:-3.11|P-value:9.24E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGL117W(YGL117W_p)|FD-Score:4.07|P-value:2.32E-5||SGD DESC:Putative protein of unknown function Gene:YIL029C(YIL029C_p)|FD-Score:-3.89|P-value:4.94E-5||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YIR024C(YIR024C)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect Gene:YJL120W(YJL120W_d)|FD-Score:-3.14|P-value:8.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YNL144C(YNL144C_p)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study Gene:YOR331C(YOR331C_d)|FD-Score:-3.73|P-value:9.62E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W Gene:YPR012W(YPR012W_d)|FD-Score:-4|P-value:3.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:YPR096C(YPR096C)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YPR097W(YPR097W)|FD-Score:5.06|P-value:2.14E-7||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR117W(YPR117W_p)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR210W4.769.44E-70.35RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YOL066C4.721.17E-60.35RIB2Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs
YNL132W4.641.73E-60.35KRE33Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
YDL193W4.395.72E-60.35NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YGR024C4.131.83E-50.35THG1tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes
YBR236C3.817.01E-50.35ABD1Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA
YLR355C3.601.58E-40.35ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YPR169W3.255.83E-40.02JIP5Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein
YKR071C3.226.33E-40.02DRE2Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress
YPL217C3.206.85E-40.00BMS1GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p
YIL026C3.206.91E-40.17IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YDL148C3.020.001250.06NOP14Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
YIL129C2.960.001540.03TAO3Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p
YGL171W2.930.001710.19ROK1RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation
YHR062C2.730.003120.01RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR354W19.909.92E-89TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YDR008C_d15.501.19E-54YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YEL009C13.204.95E-40GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YER090W12.503.53E-36TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YHR098C12.407.33E-36SFB3Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p
YDR007W12.402.32E-35TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YKL211C12.001.73E-33TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YFR009W10.408.69E-26GCN20Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
YGL026C10.409.45E-26TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YDR283C9.325.68E-21GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YDR035W7.706.79E-15ARO33-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
YKR026C6.641.60E-11GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YDR071C6.572.56E-11PAA1Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication
YHL043W5.872.18E-9ECM34Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
YPR135W5.822.99E-9CTF4Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion

GO enrichment analysis for SGTC_51
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.5470SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0533333
0.5470SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0533333
0.5210SGTC_18825615643 20.0 μMMiscellaneous22530750.166667TRP & mitochondrial translation
0.5180SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.05
0.5140SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.0853659
0.5140SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.1875
0.5110SGTC_24885268135 45.7 μMMiscellaneous12703560.237288
0.5090SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0705882
0.4890SGTC_6770929-0063 136.0 μMChemDiv (Drug-like library)15395830.190476
0.4830SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0217391
0.4800SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0394737
0.4790SGTC_18815652484 16.0 μMMiscellaneous22551290.216216TRP & mitochondrial translation
0.4780SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.326923TRP & mitochondrial translation
0.4720SGTC_1673st016611 27.3 μMTimTec (Natural product derivative library)52267990.0481928
0.4700SGTC_24785763493 32.3 μMMiscellaneous13655760.147059

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_18835-benzylidenerhodanine20 μM0.3269231273210Miscellaneous221.298682.84113TRP & mitochondrial translation
SGTC_24545-benzylidenerhodanine10.36 μM0.3269231273210Miscellaneous221.298682.84113TRP & mitochondrial translation
SGTC_2450573973712.93 μM0.2656252262825Miscellaneous328.3607233.79315
SGTC_2453573113222.18 μM0.2656251362040Miscellaneous436.266794.16614
SGTC_2468598776572.9 μM0.2526772094Miscellaneous272.322282.45413
SGTC_2472537765249.4 μM0.2459021610903Miscellaneous261.362543.75513TRP & mitochondrial translation
SGTC_2486528320222.39 μM0.2456141377751Miscellaneous231.270362.4113
SGTC_500929-007856.39 μM0.2456141377751ChemDiv (Drug-like library)231.270362.4113
SGTC_7670929-002386 μM0.2380951204563ChemDiv (Drug-like library)279.334762.69615TRP & mitochondrial translation
SGTC_3193911193149.47 μM0.23809517087244Chembridge (Drug-like library)348.191263.79713
SGTC_380929-005158.27 μM0.2372881379719ChemDiv (Drug-like library)223.2235432.14714