r062-0005

3-chloro-5-methylpyridazino[3,4-b][1,4]benzoxazine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_521
Screen concentration 13.8 μM
Source ChemDiv (Drug-like library)
PubChem CID 2832033
SMILES CN1C2=CC=CC=C2OC3=NN=C(C=C31)Cl
Standardized SMILES CN1c2ccccc2Oc3nnc(Cl)cc13
Molecular weight 233.6537
ALogP 3.11
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 16.7
% growth inhibition (Hom. pool) 7.71


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2832033
Download HIP data (tab-delimited text)  (excel)
Gene:ALG1(YBR110W)|FD-Score:-5.39|P-value:3.45E-8|Clearance:0||SGD DESC:Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog Gene:BDP1(YNL039W)|FD-Score:3.11|P-value:9.44E-4|Clearance:0.07||SGD DESC:Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs Gene:DIB1(YPR082C)|FD-Score:3.58|P-value:1.70E-4|Clearance:0.07||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:ERG20(YJL167W)|FD-Score:4.06|P-value:2.42E-5|Clearance:0.33||SGD DESC:Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis Gene:IPI3(YNL182C)|FD-Score:3.24|P-value:6.00E-4|Clearance:0.13||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene Gene:IPP1(YBR011C)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.27||SGD DESC:Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase Gene:LSG1(YGL099W)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.04||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MES1(YGR264C)|FD-Score:3.66|P-value:1.26E-4|Clearance:0.04||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:PCF11(YDR228C)|FD-Score:-3.27|P-value:5.37E-4|Clearance:0||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:PGS1(YCL004W)|FD-Score:-3.88|P-value:5.16E-5|Clearance:0||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PRP19(YLL036C)|FD-Score:-4.77|P-value:9.30E-7|Clearance:0||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:RPS20(YHL015W)|FD-Score:-3.57|P-value:1.81E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:SLN1(YIL147C)|FD-Score:-3.85|P-value:5.95E-5|Clearance:0||SGD DESC:Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators Gene:TEL2(YGR099W)|FD-Score:3.99|P-value:3.28E-5|Clearance:0.33||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TRS130(YMR218C)|FD-Score:-4.18|P-value:1.44E-5|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy Gene:ALG1(YBR110W)|FD-Score:-5.39|P-value:3.45E-8|Clearance:0||SGD DESC:Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog Gene:BDP1(YNL039W)|FD-Score:3.11|P-value:9.44E-4|Clearance:0.07||SGD DESC:Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs Gene:DIB1(YPR082C)|FD-Score:3.58|P-value:1.70E-4|Clearance:0.07||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:ERG20(YJL167W)|FD-Score:4.06|P-value:2.42E-5|Clearance:0.33||SGD DESC:Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis Gene:IPI3(YNL182C)|FD-Score:3.24|P-value:6.00E-4|Clearance:0.13||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene Gene:IPP1(YBR011C)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.27||SGD DESC:Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase Gene:LSG1(YGL099W)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.04||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MES1(YGR264C)|FD-Score:3.66|P-value:1.26E-4|Clearance:0.04||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:PCF11(YDR228C)|FD-Score:-3.27|P-value:5.37E-4|Clearance:0||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:PGS1(YCL004W)|FD-Score:-3.88|P-value:5.16E-5|Clearance:0||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PRP19(YLL036C)|FD-Score:-4.77|P-value:9.30E-7|Clearance:0||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:RPS20(YHL015W)|FD-Score:-3.57|P-value:1.81E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:SLN1(YIL147C)|FD-Score:-3.85|P-value:5.95E-5|Clearance:0||SGD DESC:Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators Gene:TEL2(YGR099W)|FD-Score:3.99|P-value:3.28E-5|Clearance:0.33||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TRS130(YMR218C)|FD-Score:-4.18|P-value:1.44E-5|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2832033
Download HOP data (tab-delimited text)  (excel)
Gene:ADD37(YMR184W)|FD-Score:-5.78|P-value:3.64E-9||SGD DESC:Protein of unknown function; involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:AKR1(YDR264C)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ARG3(YJL088W)|FD-Score:-3.86|P-value:5.65E-5||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ASC1(YMR116C)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:AUS1(YOR011W)|FD-Score:-3.26|P-value:5.54E-4||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:BDF1(YLR399C)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BNA1(YJR025C)|FD-Score:-3.8|P-value:7.19E-5||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BZZ1(YHR114W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins Gene:CAT8(YMR280C)|FD-Score:-3.2|P-value:6.89E-4||SGD DESC:Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress Gene:CIN8(YEL061C)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CLB2(YPR119W)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CPA1(YOR303W)|FD-Score:-3.17|P-value:7.67E-4||SGD DESC:Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader Gene:DUS4(YLR405W)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:FRA2(YGL220W)|FD-Score:-4.27|P-value:9.97E-6||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:GDS1(YOR355W)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:HNT3(YOR258W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:LPP1(YDR503C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Lipid phosphate phosphatase, catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA Gene:MCX1(YBR227C)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MHF1(YOL086W-A)|FD-Score:-4.02|P-value:2.89E-5||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 Gene:MIC14(YDR031W)|FD-Score:-5.32|P-value:5.19E-8||SGD DESC:Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MMT1(YMR177W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p Gene:MNN1(YER001W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family Gene:MSB2(YGR014W)|FD-Score:6.41|P-value:7.28E-11||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSG5(YNL053W)|FD-Score:-3.14|P-value:8.49E-4||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:MSN5(YDR335W)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:NRG2(YBR066C)|FD-Score:-3.89|P-value:5.06E-5||SGD DESC:Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; NRG2 has a paralog, NRG1, that arose from the whole genome duplication Gene:NTE1(YML059C)|FD-Score:-4.68|P-value:1.41E-6||SGD DESC:Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes Gene:NTO1(YPR031W)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:PEX21(YGR239C)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; partially redundant with Pex18p; relative distribution to cytoplasmic foci increases upon DNA replication stress Gene:PGM3(YMR278W)|FD-Score:-3.26|P-value:5.64E-4||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PIN4(YBL051C)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PML1(YLR016C)|FD-Score:-3.19|P-value:7.17E-4||SGD DESC:Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p Gene:POR2(YIL114C)|FD-Score:-3.76|P-value:8.57E-5||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:RAD55(YDR076W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RBG2(YGR173W)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein Gene:RKM5(YLR137W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species Gene:RMD6(YEL072W)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Protein required for sporulation Gene:RPL20A(YMR242C)|FD-Score:4.47|P-value:3.92E-6||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL26B(YGR034W)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL31B(YLR406C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:RPS0A(YGR214W)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:RRP8(YDR083W)|FD-Score:5.13|P-value:1.44E-7||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:RRT1(YBL048W_d)|FD-Score:4.75|P-value:1.00E-6||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:SIP4(YJL089W)|FD-Score:-5.45|P-value:2.50E-8||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SPO74(YGL170C)|FD-Score:4.41|P-value:5.25E-6||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SRL2(YLR082C)|FD-Score:-4.7|P-value:1.33E-6||SGD DESC:Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation Gene:TDA10(YGR205W)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele Gene:TOS4(YLR183C)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; expression is periodic and peaks in G1; relative distribution to the nucleus increases upon DNA replication stress; TOS4 has a paralog, PLM2, that arose from the whole genome duplication Gene:TPC1(YGR096W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family Gene:TRK2(YKR050W)|FD-Score:4.58|P-value:2.28E-6||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; TRK2 has a paralog, TRK1, that arose from the whole genome duplication Gene:TRP4(YDR354W)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VPS65(YLR322W_d)|FD-Score:7.63|P-value:1.21E-14||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:WHI5(YOR083W)|FD-Score:-3.87|P-value:5.41E-5||SGD DESC:Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1 Gene:YBP2(YGL060W)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YBR056W(YBR056W_p)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YDL172C(YDL172C_d)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL180W(YDL180W_p)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR095C(YDR095C_d)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR222W(YDR222W_p)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YER085C(YER085C_p)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Putative protein of unknown function Gene:YGL218W(YGL218W_d)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR045C(YGR045C_d)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR126W(YGR126W_p)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YGR130C(YGR130C)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YIL089W(YIL089W)|FD-Score:-6.45|P-value:5.42E-11||SGD DESC:Protein of unknown function found in the ER and vacuole lumen; overexpression of YIL089W affects endocytic protein trafficking Gene:YIL108W(YIL108W_p)|FD-Score:-4.33|P-value:7.53E-6||SGD DESC:Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress Gene:YIP4(YGL198W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YKL077W(YKL077W_p)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YKL096C-B(YKL096C-B_p)|FD-Score:-3.75|P-value:8.92E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YKL097C(YKL097C_d)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species Gene:YKL202W(YKL202W_d)|FD-Score:3.84|P-value:6.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR023W(YKR023W_p)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR072W(YLR072W_p)|FD-Score:-4.02|P-value:2.91E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene Gene:YLR287C(YLR287C_p)|FD-Score:5.33|P-value:4.83E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YMR124W(YMR124W_p)|FD-Score:6.06|P-value:6.87E-10||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization Gene:YNL162W-A(YNL162W-A_p)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Putative protein of unknown function; identified by homology Gene:YOR225W(YOR225W_d)|FD-Score:4.62|P-value:1.90E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR078C(YPR078C_p)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible Gene:YPR170C(YPR170C_d)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:ADD37(YMR184W)|FD-Score:-5.78|P-value:3.64E-9||SGD DESC:Protein of unknown function; involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:AKR1(YDR264C)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ARG3(YJL088W)|FD-Score:-3.86|P-value:5.65E-5||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ASC1(YMR116C)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:AUS1(YOR011W)|FD-Score:-3.26|P-value:5.54E-4||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:BDF1(YLR399C)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BNA1(YJR025C)|FD-Score:-3.8|P-value:7.19E-5||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BZZ1(YHR114W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins Gene:CAT8(YMR280C)|FD-Score:-3.2|P-value:6.89E-4||SGD DESC:Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress Gene:CIN8(YEL061C)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CLB2(YPR119W)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CPA1(YOR303W)|FD-Score:-3.17|P-value:7.67E-4||SGD DESC:Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader Gene:DUS4(YLR405W)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:FRA2(YGL220W)|FD-Score:-4.27|P-value:9.97E-6||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:GDS1(YOR355W)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:HNT3(YOR258W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:LPP1(YDR503C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Lipid phosphate phosphatase, catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA Gene:MCX1(YBR227C)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MHF1(YOL086W-A)|FD-Score:-4.02|P-value:2.89E-5||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 Gene:MIC14(YDR031W)|FD-Score:-5.32|P-value:5.19E-8||SGD DESC:Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MMT1(YMR177W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p Gene:MNN1(YER001W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family Gene:MSB2(YGR014W)|FD-Score:6.41|P-value:7.28E-11||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSG5(YNL053W)|FD-Score:-3.14|P-value:8.49E-4||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:MSN5(YDR335W)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:NRG2(YBR066C)|FD-Score:-3.89|P-value:5.06E-5||SGD DESC:Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; NRG2 has a paralog, NRG1, that arose from the whole genome duplication Gene:NTE1(YML059C)|FD-Score:-4.68|P-value:1.41E-6||SGD DESC:Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes Gene:NTO1(YPR031W)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:PEX21(YGR239C)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; partially redundant with Pex18p; relative distribution to cytoplasmic foci increases upon DNA replication stress Gene:PGM3(YMR278W)|FD-Score:-3.26|P-value:5.64E-4||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PIN4(YBL051C)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PML1(YLR016C)|FD-Score:-3.19|P-value:7.17E-4||SGD DESC:Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p Gene:POR2(YIL114C)|FD-Score:-3.76|P-value:8.57E-5||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:RAD55(YDR076W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RBG2(YGR173W)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein Gene:RKM5(YLR137W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species Gene:RMD6(YEL072W)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Protein required for sporulation Gene:RPL20A(YMR242C)|FD-Score:4.47|P-value:3.92E-6||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL26B(YGR034W)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL31B(YLR406C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:RPS0A(YGR214W)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:RRP8(YDR083W)|FD-Score:5.13|P-value:1.44E-7||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:RRT1(YBL048W_d)|FD-Score:4.75|P-value:1.00E-6||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:SIP4(YJL089W)|FD-Score:-5.45|P-value:2.50E-8||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SPO74(YGL170C)|FD-Score:4.41|P-value:5.25E-6||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SRL2(YLR082C)|FD-Score:-4.7|P-value:1.33E-6||SGD DESC:Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation Gene:TDA10(YGR205W)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele Gene:TOS4(YLR183C)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; expression is periodic and peaks in G1; relative distribution to the nucleus increases upon DNA replication stress; TOS4 has a paralog, PLM2, that arose from the whole genome duplication Gene:TPC1(YGR096W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family Gene:TRK2(YKR050W)|FD-Score:4.58|P-value:2.28E-6||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; TRK2 has a paralog, TRK1, that arose from the whole genome duplication Gene:TRP4(YDR354W)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VPS65(YLR322W_d)|FD-Score:7.63|P-value:1.21E-14||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:WHI5(YOR083W)|FD-Score:-3.87|P-value:5.41E-5||SGD DESC:Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1 Gene:YBP2(YGL060W)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YBR056W(YBR056W_p)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YDL172C(YDL172C_d)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL180W(YDL180W_p)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR095C(YDR095C_d)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR222W(YDR222W_p)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YER085C(YER085C_p)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Putative protein of unknown function Gene:YGL218W(YGL218W_d)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR045C(YGR045C_d)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR126W(YGR126W_p)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YGR130C(YGR130C)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YIL089W(YIL089W)|FD-Score:-6.45|P-value:5.42E-11||SGD DESC:Protein of unknown function found in the ER and vacuole lumen; overexpression of YIL089W affects endocytic protein trafficking Gene:YIL108W(YIL108W_p)|FD-Score:-4.33|P-value:7.53E-6||SGD DESC:Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress Gene:YIP4(YGL198W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YKL077W(YKL077W_p)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YKL096C-B(YKL096C-B_p)|FD-Score:-3.75|P-value:8.92E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YKL097C(YKL097C_d)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species Gene:YKL202W(YKL202W_d)|FD-Score:3.84|P-value:6.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR023W(YKR023W_p)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR072W(YLR072W_p)|FD-Score:-4.02|P-value:2.91E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene Gene:YLR287C(YLR287C_p)|FD-Score:5.33|P-value:4.83E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YMR124W(YMR124W_p)|FD-Score:6.06|P-value:6.87E-10||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization Gene:YNL162W-A(YNL162W-A_p)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Putative protein of unknown function; identified by homology Gene:YOR225W(YOR225W_d)|FD-Score:4.62|P-value:1.90E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR078C(YPR078C_p)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible Gene:YPR170C(YPR170C_d)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YJL167W4.062.42E-50.33ERG20Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis
YGR099W3.993.28E-50.33TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
YGR264C3.661.26E-40.04MES1Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs
YGL099W3.621.47E-40.04LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YPR082C3.581.70E-40.07DIB117-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein
YBR011C3.512.22E-40.27IPP1Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase
YNL182C3.246.00E-40.13IPI3Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene
YNL039W3.119.44E-40.07BDP1Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs
YDL145C3.030.001210.25COP1Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway
YJR045C2.790.002670.11SSC1Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication
YDL029W2.680.003730.00ARP2Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YGL238W2.670.003760.02CSE1Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation
YBR202W2.650.004030.04MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YDL028C2.600.004590.08MPS1Dual-specificity kinase; required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p
YLR140W_d2.530.005750.05YLR140W_dDubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR322W_d7.631.21E-14VPS65_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth
YGR014W6.417.28E-11MSB2Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate
YMR124W_p6.066.87E-10YMR124W_pProtein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
YLR287C_p5.334.83E-8YLR287C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene
YDR083W5.131.44E-7RRP8Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1
YBL048W_d4.751.00E-6RRT1_dIdentified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data
YOR225W_d4.621.90E-6YOR225W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YKR050W4.582.28E-6TRK2Component of the Trk1p-Trk2p potassium transport system; TRK2 has a paralog, TRK1, that arose from the whole genome duplication
YMR242C4.473.92E-6RPL20ARibosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication
YGL170C4.415.25E-6SPO74Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation
YGR045C_d4.241.10E-5YGR045C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR222W_p4.121.86E-5YDR222W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YEL061C4.121.91E-5CIN8Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation
YBR227C4.092.18E-5MCX1Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins
YEL072W4.042.62E-5RMD6Protein required for sporulation

GO enrichment analysis for SGTC_521
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0983.73E-14SGTC_5144466-0038 2.6 nMChemDiv (Drug-like library)57220640.140351cell wall
0.0931.04E-12SGTC_460trequinsin 123.0 μMMiscellaneous55370.0958904
0.0808.23E-10SGTC_9164466-0038 42.0 nMChemDiv (Drug-like library)57220640.140351cell wall
0.0765.79E-9SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.11111160S ribosome export
0.0715.05E-8SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.16071460S ribosome export
0.0715.22E-8SGTC_33029125395 16.4 μMChembridge (Drug-like library)175996680.126984
0.0672.86E-7SGTC_1210557-0495 61.3 μMChemDiv (Drug-like library)31044270.078125calcium & mitochondrial duress
0.0663.86E-7SGTC_29379044753 11.3 μMChembridge (Drug-like library)64655300.039473760S ribosome export
0.0664.55E-7SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.10810860S ribosome export
0.0641.03E-6SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.54166760S ribosome export
0.0621.73E-6SGTC_33099129340 25.8 μMChembridge (Drug-like library)171589870.115942
0.0622.03E-6SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.11428660S ribosome export
0.0622.64E-6SGTC_448monastrol 171.0 μMICCB bioactive library29879270.084507
0.0603.91E-6SGTC_24275545055 42.8 μMMiscellaneous53728580.118644
0.0603.97E-6SGTC_1829berberine 14.4 μMTimTec (Natural product derivative library)23530.098591660S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_970535-070119.13 μM0.5416672832013ChemDiv (Drug-like library)311.381543.2440660S ribosome export
SGTC_1915st06710530.6 μM0.369751TimTec (Natural product derivative library)209.243243.1602RNA pol III & RNase P/MRP
SGTC_21796298986200 μM0.285714738374Chembridge (Fragment library)218.235142.7312heme requiring
SGTC_13321480-065317.5 μM0.274515749438ChemDiv (Drug-like library)219.259662.79503TSC3-RPN4
SGTC_2701st07776714.72 μM0.2407415721188TimTec (Natural product derivative library)281.329044.46903
SGTC_1686st019653115.5 μM0.2391397252TimTec (Natural product derivative library)173.211141.90401mitochondrial processes
SGTC_2731amoxapine43.43 μM0.2372882170Miscellaneous313.781442.89713
SGTC_2001402350257.66 μM0.234043108941Chembridge (Fragment library)195.602441.5402mitochondrial processes
SGTC_12480469-068758.6 μM0.2321434017482ChemDiv (Drug-like library)284.223722.88805
SGTC_20335192550145 μM0.22449182073Chembridge (Fragment library)189.210541.14602