3788-1495

N-[(4-methoxyphenyl)methyl]-2,3-dihydro-1,4-benzodioxine-3-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_531
Screen concentration 174.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 2896655
SMILES COC1=CC=C(C=C1)CNC(=O)C2COC3=CC=CC=C3O2
Standardized SMILES COc1ccc(CNC(=O)C2COc3ccccc3O2)cc1
Molecular weight 299.3212
ALogP 2.38
H-bond donor count 1
H-bond acceptor count 4
Response signature unfolded protein response

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.36
% growth inhibition (Hom. pool) 14.31


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2896655
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:4.05|P-value:2.59E-5|Clearance:0.94||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:CHS2(YBR038W)|FD-Score:4.4|P-value:5.51E-6|Clearance:0.16||SGD DESC:Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis Gene:DFR1(YOR236W)|FD-Score:3.1|P-value:9.53E-4|Clearance:0.01||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:FAS1(YKL182W)|FD-Score:13.3|P-value:1.38E-40|Clearance:4.37||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GLN1(YPR035W)|FD-Score:6.12|P-value:4.55E-10|Clearance:1.11||SGD DESC:Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GPI1(YGR216C)|FD-Score:-3.32|P-value:4.44E-4|Clearance:0||SGD DESC:Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs Gene:GWT1(YJL091C)|FD-Score:10.5|P-value:4.83E-26|Clearance:4.37||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:MAK16(YAL025C)|FD-Score:-3.35|P-value:3.98E-4|Clearance:0||SGD DESC:Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus Gene:NOP8(YOL144W)|FD-Score:-3.98|P-value:3.43E-5|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PSF1(YDR013W)|FD-Score:-3.53|P-value:2.08E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:PUP2(YGR253C)|FD-Score:3.1|P-value:9.71E-4|Clearance:0.02||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:RPN11(YFR004W)|FD-Score:-3.17|P-value:7.58E-4|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPN3(YER021W)|FD-Score:-3.51|P-value:2.27E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RSC8(YFR037C)|FD-Score:5.02|P-value:2.64E-7|Clearance:0.35||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SDH3(YKL141W)|FD-Score:-4.65|P-value:1.64E-6|Clearance:0||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SEC59(YMR013C)|FD-Score:-3.55|P-value:1.96E-4|Clearance:0||SGD DESC:Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation Gene:SUI3(YPL237W)|FD-Score:4.22|P-value:1.23E-5|Clearance:0.17||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TFC3(YAL001C)|FD-Score:4.24|P-value:1.11E-5|Clearance:0.02||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:ULP1(YPL020C)|FD-Score:4.66|P-value:1.55E-6|Clearance:0.27||SGD DESC:Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions Gene:ACC1(YNR016C)|FD-Score:4.05|P-value:2.59E-5|Clearance:0.94||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:CHS2(YBR038W)|FD-Score:4.4|P-value:5.51E-6|Clearance:0.16||SGD DESC:Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis Gene:DFR1(YOR236W)|FD-Score:3.1|P-value:9.53E-4|Clearance:0.01||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:FAS1(YKL182W)|FD-Score:13.3|P-value:1.38E-40|Clearance:4.37||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GLN1(YPR035W)|FD-Score:6.12|P-value:4.55E-10|Clearance:1.11||SGD DESC:Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GPI1(YGR216C)|FD-Score:-3.32|P-value:4.44E-4|Clearance:0||SGD DESC:Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs Gene:GWT1(YJL091C)|FD-Score:10.5|P-value:4.83E-26|Clearance:4.37||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:MAK16(YAL025C)|FD-Score:-3.35|P-value:3.98E-4|Clearance:0||SGD DESC:Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus Gene:NOP8(YOL144W)|FD-Score:-3.98|P-value:3.43E-5|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PSF1(YDR013W)|FD-Score:-3.53|P-value:2.08E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:PUP2(YGR253C)|FD-Score:3.1|P-value:9.71E-4|Clearance:0.02||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:RPN11(YFR004W)|FD-Score:-3.17|P-value:7.58E-4|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPN3(YER021W)|FD-Score:-3.51|P-value:2.27E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RSC8(YFR037C)|FD-Score:5.02|P-value:2.64E-7|Clearance:0.35||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SDH3(YKL141W)|FD-Score:-4.65|P-value:1.64E-6|Clearance:0||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SEC59(YMR013C)|FD-Score:-3.55|P-value:1.96E-4|Clearance:0||SGD DESC:Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation Gene:SUI3(YPL237W)|FD-Score:4.22|P-value:1.23E-5|Clearance:0.17||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TFC3(YAL001C)|FD-Score:4.24|P-value:1.11E-5|Clearance:0.02||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:ULP1(YPL020C)|FD-Score:4.66|P-value:1.55E-6|Clearance:0.27||SGD DESC:Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2896655
Download HOP data (tab-delimited text)  (excel)
Gene:ADH2(YMR303C)|FD-Score:4.43|P-value:4.69E-6||SGD DESC:Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 Gene:ALG12(YNR030W)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:APE1(YKL103C)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Vacuolar aminopeptidase yscI; zinc metalloproteinase that belongs to the peptidase family M18; often used as a marker protein in studies of autophagy and cytosol to vacuole targeting (CVT) pathway; protein increases in abundance and relative distribution to cytoplasmic foci increases upon DNA replication stress Gene:BCK1(YJL095W)|FD-Score:11.8|P-value:1.87E-32||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CDC73(YLR418C)|FD-Score:4.51|P-value:3.23E-6||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLB2(YPR119W)|FD-Score:5.37|P-value:3.95E-8||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CNE1(YAL058W)|FD-Score:6.21|P-value:2.64E-10||SGD DESC:Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast Gene:CWH41(YGL027C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DAK1(YML070W)|FD-Score:-5.34|P-value:4.57E-8||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DBP7(YKR024C)|FD-Score:4.85|P-value:6.18E-7||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:ERG4(YGL012W)|FD-Score:3.78|P-value:7.80E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FMP52(YER004W)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FPR1(YNL135C)|FD-Score:-3.13|P-value:8.61E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:FYV8(YGR196C)|FD-Score:6.37|P-value:9.20E-11||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GAS3(YMR215W)|FD-Score:3.75|P-value:8.88E-5||SGD DESC:Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation Gene:GET1(YGL020C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GEX2(YKR106W)|FD-Score:-4.11|P-value:1.98E-5||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GFD2(YCL036W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication Gene:GIS2(YNL255C)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:HAC1(YFL031W)|FD-Score:19.6|P-value:3.34E-86||SGD DESC:Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress Gene:HHT2(YNL031C)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation Gene:HRQ1(YDR291W_p)|FD-Score:-3.97|P-value:3.57E-5||SGD DESC:3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) Gene:IES2(YNL215W)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:IRE1(YHR079C)|FD-Score:36.9|P-value:5.69E-299||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:IST2(YBR086C)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:JIP3(YLR331C_d)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 Gene:JIP4(YDR475C)|FD-Score:3.81|P-value:6.88E-5||SGD DESC:Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence Gene:LAP2(YNL045W)|FD-Score:-3.16|P-value:7.97E-4||SGD DESC:Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:MDM31(YHR194W)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 Gene:MEP1(YGR121C)|FD-Score:-3.2|P-value:6.95E-4||SGD DESC:Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MKC7(YDR144C)|FD-Score:-4.21|P-value:1.28E-5||SGD DESC:GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p Gene:MNN11(YJL183W)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MRP20(YDR405W)|FD-Score:3.27|P-value:5.35E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS5(YBR251W)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NMA111(YNL123W)|FD-Score:-3.34|P-value:4.18E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NPR1(YNL183C)|FD-Score:-3.14|P-value:8.46E-4||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:OPI6(YDL096C_d)|FD-Score:6.27|P-value:1.80E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential Gene:OSH2(YDL019C)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAI3(YMR174C)|FD-Score:3.74|P-value:9.27E-5||SGD DESC:Cytoplasmic proteinase A (Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact Gene:PAU17(YLL025W)|FD-Score:-4.01|P-value:3.04E-5||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene Gene:PAU2(YEL049W)|FD-Score:-3.11|P-value:9.35E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PCS60(YBR222C)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase Gene:PMT1(YDL095W)|FD-Score:3.79|P-value:7.41E-5||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:PUT1(YLR142W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Proline oxidase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source Gene:QRI5(YLR204W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RDR1(YOR380W)|FD-Score:-4.3|P-value:8.71E-6||SGD DESC:Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins Gene:REG1(YDR028C)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:REX4(YOL080C)|FD-Score:-4.68|P-value:1.43E-6||SGD DESC:Putative RNA exonuclease possibly involved in pre-rRNA processing and ribosome assembly Gene:RGD1(YBR260C)|FD-Score:3.39|P-value:3.43E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization Gene:RPH1(YER169W)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPL9A(YGL147C)|FD-Score:-3.31|P-value:4.66E-4||SGD DESC:Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RPS24B(YIL069C)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24B has a paralog, RPS24A, that arose from the whole genome duplication Gene:RRT14(YIL127C_p)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:RUD3(YOR216C)|FD-Score:4.51|P-value:3.24E-6||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:SGF73(YGL066W)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SIP3(YNL257C)|FD-Score:3.79|P-value:7.59E-5||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SLA1(YBL007C)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLT2(YHR030C)|FD-Score:12|P-value:1.04E-33||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNA4(YDL123W)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated Gene:SNF4(YGL115W)|FD-Score:4.52|P-value:3.04E-6||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SNF6(YHL025W)|FD-Score:-5.85|P-value:2.49E-9||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SSP2(YOR242C)|FD-Score:-3.13|P-value:8.66E-4||SGD DESC:Sporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; SSP2 expression is induced midway in meiosis Gene:TGS1(YPL157W)|FD-Score:-3.95|P-value:3.86E-5||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:THP2(YHR167W)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TPO3(YPR156C)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication Gene:TUB3(YML124C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBP5(YER144C)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:YAL056C-A(YAL056C-A_d)|FD-Score:6.33|P-value:1.26E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAL067W-A(YAL067W-A_p)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBL008W-A(YBL008W-A_p)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBL039W-B(YBL039W-B_p)|FD-Score:-5.09|P-value:1.76E-7||SGD DESC:Putative protein of unknown function Gene:YDR541C(YDR541C_p)|FD-Score:-5.66|P-value:7.46E-9||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YER077C(YER077C_p)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YFL032W(YFL032W_d)|FD-Score:20.6|P-value:3.29E-94||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YGR151C(YGR151C_d)|FD-Score:-3.16|P-value:7.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YIL086C(YIL086C_d)|FD-Score:-4.05|P-value:2.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR084W(YJR084W)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YKL033W-A(YKL033W-A_p)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YLL053C(YLL053C_p)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YLR177W(YLR177W_p)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication Gene:YML047W-A(YML047W-A_d)|FD-Score:-3.33|P-value:4.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL010W(YNL010W_p)|FD-Score:-3.16|P-value:7.84E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNL211C(YNL211C_p)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YOR304C-A(YOR304C-A_p)|FD-Score:3.89|P-value:4.94E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:ADH2(YMR303C)|FD-Score:4.43|P-value:4.69E-6||SGD DESC:Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 Gene:ALG12(YNR030W)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:APE1(YKL103C)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Vacuolar aminopeptidase yscI; zinc metalloproteinase that belongs to the peptidase family M18; often used as a marker protein in studies of autophagy and cytosol to vacuole targeting (CVT) pathway; protein increases in abundance and relative distribution to cytoplasmic foci increases upon DNA replication stress Gene:BCK1(YJL095W)|FD-Score:11.8|P-value:1.87E-32||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CDC73(YLR418C)|FD-Score:4.51|P-value:3.23E-6||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLB2(YPR119W)|FD-Score:5.37|P-value:3.95E-8||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CNE1(YAL058W)|FD-Score:6.21|P-value:2.64E-10||SGD DESC:Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast Gene:CWH41(YGL027C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DAK1(YML070W)|FD-Score:-5.34|P-value:4.57E-8||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DBP7(YKR024C)|FD-Score:4.85|P-value:6.18E-7||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:ERG4(YGL012W)|FD-Score:3.78|P-value:7.80E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FMP52(YER004W)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FPR1(YNL135C)|FD-Score:-3.13|P-value:8.61E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:FYV8(YGR196C)|FD-Score:6.37|P-value:9.20E-11||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GAS3(YMR215W)|FD-Score:3.75|P-value:8.88E-5||SGD DESC:Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation Gene:GET1(YGL020C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GEX2(YKR106W)|FD-Score:-4.11|P-value:1.98E-5||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GFD2(YCL036W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication Gene:GIS2(YNL255C)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:HAC1(YFL031W)|FD-Score:19.6|P-value:3.34E-86||SGD DESC:Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress Gene:HHT2(YNL031C)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation Gene:HRQ1(YDR291W_p)|FD-Score:-3.97|P-value:3.57E-5||SGD DESC:3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) Gene:IES2(YNL215W)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:IRE1(YHR079C)|FD-Score:36.9|P-value:5.69E-299||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:IST2(YBR086C)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:JIP3(YLR331C_d)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 Gene:JIP4(YDR475C)|FD-Score:3.81|P-value:6.88E-5||SGD DESC:Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence Gene:LAP2(YNL045W)|FD-Score:-3.16|P-value:7.97E-4||SGD DESC:Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:MDM31(YHR194W)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 Gene:MEP1(YGR121C)|FD-Score:-3.2|P-value:6.95E-4||SGD DESC:Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MKC7(YDR144C)|FD-Score:-4.21|P-value:1.28E-5||SGD DESC:GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p Gene:MNN11(YJL183W)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MRP20(YDR405W)|FD-Score:3.27|P-value:5.35E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS5(YBR251W)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NMA111(YNL123W)|FD-Score:-3.34|P-value:4.18E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NPR1(YNL183C)|FD-Score:-3.14|P-value:8.46E-4||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:OPI6(YDL096C_d)|FD-Score:6.27|P-value:1.80E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential Gene:OSH2(YDL019C)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAI3(YMR174C)|FD-Score:3.74|P-value:9.27E-5||SGD DESC:Cytoplasmic proteinase A (Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact Gene:PAU17(YLL025W)|FD-Score:-4.01|P-value:3.04E-5||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene Gene:PAU2(YEL049W)|FD-Score:-3.11|P-value:9.35E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PCS60(YBR222C)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase Gene:PMT1(YDL095W)|FD-Score:3.79|P-value:7.41E-5||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:PUT1(YLR142W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Proline oxidase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source Gene:QRI5(YLR204W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RDR1(YOR380W)|FD-Score:-4.3|P-value:8.71E-6||SGD DESC:Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins Gene:REG1(YDR028C)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:REX4(YOL080C)|FD-Score:-4.68|P-value:1.43E-6||SGD DESC:Putative RNA exonuclease possibly involved in pre-rRNA processing and ribosome assembly Gene:RGD1(YBR260C)|FD-Score:3.39|P-value:3.43E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization Gene:RPH1(YER169W)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPL9A(YGL147C)|FD-Score:-3.31|P-value:4.66E-4||SGD DESC:Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RPS24B(YIL069C)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24B has a paralog, RPS24A, that arose from the whole genome duplication Gene:RRT14(YIL127C_p)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:RUD3(YOR216C)|FD-Score:4.51|P-value:3.24E-6||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:SGF73(YGL066W)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SIP3(YNL257C)|FD-Score:3.79|P-value:7.59E-5||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SLA1(YBL007C)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLT2(YHR030C)|FD-Score:12|P-value:1.04E-33||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNA4(YDL123W)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated Gene:SNF4(YGL115W)|FD-Score:4.52|P-value:3.04E-6||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SNF6(YHL025W)|FD-Score:-5.85|P-value:2.49E-9||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SSP2(YOR242C)|FD-Score:-3.13|P-value:8.66E-4||SGD DESC:Sporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; SSP2 expression is induced midway in meiosis Gene:TGS1(YPL157W)|FD-Score:-3.95|P-value:3.86E-5||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:THP2(YHR167W)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TPO3(YPR156C)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication Gene:TUB3(YML124C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBP5(YER144C)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:YAL056C-A(YAL056C-A_d)|FD-Score:6.33|P-value:1.26E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAL067W-A(YAL067W-A_p)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBL008W-A(YBL008W-A_p)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBL039W-B(YBL039W-B_p)|FD-Score:-5.09|P-value:1.76E-7||SGD DESC:Putative protein of unknown function Gene:YDR541C(YDR541C_p)|FD-Score:-5.66|P-value:7.46E-9||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YER077C(YER077C_p)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YFL032W(YFL032W_d)|FD-Score:20.6|P-value:3.29E-94||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YGR151C(YGR151C_d)|FD-Score:-3.16|P-value:7.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YIL086C(YIL086C_d)|FD-Score:-4.05|P-value:2.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR084W(YJR084W)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YKL033W-A(YKL033W-A_p)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YLL053C(YLL053C_p)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YLR177W(YLR177W_p)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication Gene:YML047W-A(YML047W-A_d)|FD-Score:-3.33|P-value:4.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL010W(YNL010W_p)|FD-Score:-3.16|P-value:7.84E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNL211C(YNL211C_p)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YOR304C-A(YOR304C-A_p)|FD-Score:3.89|P-value:4.94E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL182W13.301.38E-404.37FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YJL091C10.504.83E-264.37GWT1Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors
YPR035W6.124.55E-101.11GLN1Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YFR037C5.022.64E-70.35RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YPL020C4.661.55E-60.27ULP1Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions
YBR038W4.405.51E-60.15CHS2Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis
YAL001C4.241.11E-50.02TFC3Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding
YPL237W4.221.23E-50.17SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
YNR016C4.052.59E-50.94ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YOR236W3.109.53E-40.01DFR1Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
YGR253C3.109.71E-40.02PUP2Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
YPL242C3.080.001030.05IQG1Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress
YPR180W3.030.001210.00AOS1Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
YKL111C_d3.030.001232.25E-4YKL111C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1
YLR438C-A3.030.001230.03LSM3Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR079C36.905.69E-299IRE1Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress
YFL032W_d20.603.29E-94YFL032W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene
YFL031W19.603.34E-86HAC1Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress
YHR030C12.001.04E-33SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YJL095W11.801.87E-32BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YGR196C6.379.20E-11FYV8Protein of unknown function, required for survival upon exposure to K1 killer toxin
YAL056C-A_d6.331.26E-10YAL056C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDL096C_d6.271.80E-10OPI6_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
YAL058W6.212.64E-10CNE1Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
YPR119W5.373.95E-8CLB2B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome
YKR024C4.856.18E-7DBP7Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions
YGL115W4.523.04E-6SNF4Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress
YLR418C4.513.23E-6CDC73Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress
YOR216C4.513.24E-6RUD3Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1
YMR303C4.434.69E-6ADH2Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1

GO enrichment analysis for SGTC_531
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.5260SGTC_3531988-1175 58.3 μMChemDiv (Drug-like library)27883570.75unfolded protein response
0.4446.44E-284SGTC_220964-0034 14.5 μMChemDiv (Drug-like library)1384020.016129unfolded protein response
0.4306.39E-265SGTC_12390337-0584 35.4 μMChemDiv (Drug-like library)9143010.125unfolded protein response
0.4132.95E-242SGTC_29979067075 71.4 μMChembridge (Drug-like library)272098190.188406unfolded protein response
0.3793.81E-201SGTC_30789116413 49.5 μMChembridge (Drug-like library)171773220.170732unfolded protein response
0.3692.39E-190SGTC_160428-0027 22.8 μMChemDiv (Drug-like library)39673390.114754unfolded protein response
0.3672.98E-187SGTC_255tunicamycin 200.5 nMICCB bioactive library238471770.102804unfolded protein response
0.2896.90E-114SGTC_112phenothiazine 287.2 μMChemDiv (Drug-like library)71080.0925926unfolded protein response
0.2631.19E-93SGTC_1080509-0257 621.1 μMChemDiv (Drug-like library)5366240.16129
0.2271.37E-69SGTC_30601327-0027 12.5 μMChemDiv (Drug-like library)160004550.148649
0.2251.80E-68SGTC_6631486-0733 13.9 μMChemDiv (Drug-like library)7224450.109756cell wall signaling
0.2125.02E-61SGTC_22727805186 180.0 μMChembridge (Fragment library)29560620.130435cell wall signaling
0.2111.46E-60SGTC_20074011539 71.8 μMChembridge (Fragment library)455953010.174603cell wall signaling
0.2101.33E-59SGTC_30631327-0034 4.0 μMChemDiv (Drug-like library)159985800.0897436
0.1971.39E-52SGTC_20094012297 12.1 μMChembridge (Fragment library)455953070.119403cell wall signaling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3531988-117558.3 μM0.752788357ChemDiv (Drug-like library)269.29522.39413unfolded protein response
SGTC_3135912774249.47 μM0.475413643468Chembridge (Drug-like library)427.45041.77316
SGTC_2851901592271.43 μM0.389832996893Chembridge (Drug-like library)299.364243.31513
SGTC_1970st07012235.8 μM0.350877102928TimTec (Natural product derivative library)254.280563.08303TSC3-RPN4
SGTC_14003771-899851.5 μM0.34375670767ChemDiv (Drug-like library)299.321182.72214
SGTC_2039Aminomethylbenzodioxan200 μM0.3415053Chembridge (Fragment library)165.189140.81513
SGTC_10204335-096193.3 μM0.333333669075ChemDiv (Drug-like library)267.322382.91302
SGTC_9073448-698675.5 μM0.328358590582ChemDiv (Drug-like library)352.307813.35126
SGTC_7033875-007571.8 μM0.3239442850814ChemDiv (Drug-like library)348.373642.05217
SGTC_3039909027049.47 μM0.31666717173750Chembridge (Drug-like library)303.740263.07113RPP1 & pyrimidine depletion