1548-0774

N-[2-[(2Z)-2-(1-benzyl-2-oxoindol-3-ylidene)hydrazinyl]-2-oxoethyl]thiophene-2-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_532
Screen concentration 215.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 5932993
SMILES C1=CC=C(C=C1)CN2C3=CC=CC=C3C(=NNC(=O)CNC(=O)C4=CC=CS4)C2=O
Standardized SMILES Oc1c(N=NC(=O)CNC(=O)c2cccs2)c3ccccc3n1Cc4ccccc4
Molecular weight 418.4683
ALogP 2.57
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.51
% growth inhibition (Hom. pool) 4.65


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5932993
Download HIP data (tab-delimited text)  (excel)
Gene:BET1(YIL004C)|FD-Score:4.83|P-value:6.99E-7|Clearance:0.73||SGD DESC:Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins Gene:BRL1(YHR036W)|FD-Score:3.15|P-value:8.29E-4|Clearance:0.03||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CHS2(YBR038W)|FD-Score:3.09|P-value:9.84E-4|Clearance:0.03||SGD DESC:Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis Gene:CWC22(YGR278W)|FD-Score:-3.8|P-value:7.24E-5|Clearance:0||SGD DESC:Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein Gene:DUO1(YGL061C)|FD-Score:5.27|P-value:6.70E-8|Clearance:0.45||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:FOL2(YGR267C)|FD-Score:-3.11|P-value:9.41E-4|Clearance:0||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:NOP56(YLR197W)|FD-Score:3.92|P-value:4.44E-5|Clearance:0.35||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NPA3(YJR072C)|FD-Score:-3.55|P-value:1.89E-4|Clearance:0||SGD DESC:Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress Gene:NUG1(YER006W)|FD-Score:3.57|P-value:1.76E-4|Clearance:0.02||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:NUP145(YGL092W)|FD-Score:4.1|P-value:2.07E-5|Clearance:0.18||SGD DESC:Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 Gene:RPC19(YNL113W)|FD-Score:3.12|P-value:9.04E-4|Clearance:0.03||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPT5(YOR117W)|FD-Score:3.55|P-value:1.93E-4|Clearance:0.23||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:SMD1(YGR074W)|FD-Score:3.32|P-value:4.58E-4|Clearance:0.05||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:UTP20(YBL004W)|FD-Score:3.27|P-value:5.41E-4|Clearance:0.12||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:UTP25(YIL091C)|FD-Score:-3.77|P-value:8.07E-5|Clearance:0||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:YDL163W(YDL163W_d)|FD-Score:6.41|P-value:7.19E-11|Clearance:1.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:BET1(YIL004C)|FD-Score:4.83|P-value:6.99E-7|Clearance:0.73||SGD DESC:Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins Gene:BRL1(YHR036W)|FD-Score:3.15|P-value:8.29E-4|Clearance:0.03||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CHS2(YBR038W)|FD-Score:3.09|P-value:9.84E-4|Clearance:0.03||SGD DESC:Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis Gene:CWC22(YGR278W)|FD-Score:-3.8|P-value:7.24E-5|Clearance:0||SGD DESC:Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein Gene:DUO1(YGL061C)|FD-Score:5.27|P-value:6.70E-8|Clearance:0.45||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:FOL2(YGR267C)|FD-Score:-3.11|P-value:9.41E-4|Clearance:0||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:NOP56(YLR197W)|FD-Score:3.92|P-value:4.44E-5|Clearance:0.35||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NPA3(YJR072C)|FD-Score:-3.55|P-value:1.89E-4|Clearance:0||SGD DESC:Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress Gene:NUG1(YER006W)|FD-Score:3.57|P-value:1.76E-4|Clearance:0.02||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:NUP145(YGL092W)|FD-Score:4.1|P-value:2.07E-5|Clearance:0.18||SGD DESC:Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 Gene:RPC19(YNL113W)|FD-Score:3.12|P-value:9.04E-4|Clearance:0.03||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPT5(YOR117W)|FD-Score:3.55|P-value:1.93E-4|Clearance:0.23||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:SMD1(YGR074W)|FD-Score:3.32|P-value:4.58E-4|Clearance:0.05||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:UTP20(YBL004W)|FD-Score:3.27|P-value:5.41E-4|Clearance:0.12||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:UTP25(YIL091C)|FD-Score:-3.77|P-value:8.07E-5|Clearance:0||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:YDL163W(YDL163W_d)|FD-Score:6.41|P-value:7.19E-11|Clearance:1.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5932993
Download HOP data (tab-delimited text)  (excel)
Gene:ABM1(YJR108W)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Protein of unknown function, required for normal microtubule organization Gene:ADE12(YNL220W)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:AHC2(YCR082W)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:AIM4(YBR194W)|FD-Score:4.27|P-value:9.91E-6||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:ATG27(YJL178C)|FD-Score:5.57|P-value:1.27E-8||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:BCH2(YKR027W)|FD-Score:3.72|P-value:9.96E-5||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BRE1(YDL074C)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BRE2(YLR015W)|FD-Score:-3.11|P-value:9.27E-4||SGD DESC:Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L Gene:CIN5(YOR028C)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication Gene:CNE1(YAL058W)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast Gene:DPP1(YDR284C)|FD-Score:5.22|P-value:8.84E-8||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:DSS1(YMR287C)|FD-Score:3.95|P-value:3.83E-5||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:GET3(YDL100C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GEX2(YKR106W)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GLC8(YMR311C)|FD-Score:-3.4|P-value:3.32E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GYP8(YFL027C)|FD-Score:-4.14|P-value:1.77E-5||SGD DESC:GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport Gene:HDA3(YPR179C)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p Gene:HST1(YOL068C)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:ICL2(YPR006C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol Gene:ISC1(YER019W)|FD-Score:4.32|P-value:7.66E-6||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:KES1(YPL145C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:LAA1(YJL207C)|FD-Score:-4.08|P-value:2.21E-5||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:MAG1(YER142C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress Gene:MAM33(YIL070C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R Gene:MDG1(YNL173C)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MET32(YDR253C)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MNE1(YOR350C)|FD-Score:3.92|P-value:4.48E-5||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MRF1(YGL143C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MSI1(YBR195C)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:NUM1(YDR150W)|FD-Score:5.7|P-value:5.98E-9||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:PCS60(YBR222C)|FD-Score:-4.05|P-value:2.56E-5||SGD DESC:Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase Gene:PET309(YLR067C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PRE9(YGR135W)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform Gene:PSY3(YLR376C)|FD-Score:3.77|P-value:8.06E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:PUT4(YOR348C)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells Gene:PYC1(YGL062W)|FD-Score:4.41|P-value:5.13E-6||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:RAD16(YBR114W)|FD-Score:5.99|P-value:1.03E-9||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RAD28(YDR030C)|FD-Score:5.13|P-value:1.47E-7||SGD DESC:Protein involved in DNA repair, related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair Gene:RFX1(YLR176C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RGC1(YPR115W)|FD-Score:4.56|P-value:2.60E-6||SGD DESC:Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication Gene:RPL13A(YDL082W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:SFM1(YOR021C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism Gene:SGA1(YIL099W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Intracellular sporulation-specific glucoamylase involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation Gene:SIP3(YNL257C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SIZ1(YDR409W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:TDA11(YHR159W_p)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TIF4632(YGL049C)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication Gene:VMA7(YGR020C)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:YAL067W-A(YAL067W-A_p)|FD-Score:-4.3|P-value:8.44E-6||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCH1(YGR203W)|FD-Score:3.89|P-value:4.92E-5||SGD DESC:Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YDR179W-A(YDR179W-A_p)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Putative protein of unknown function Gene:YDR209C(YDR209C_d)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W. Gene:YER053C-A(YER053C-A_p)|FD-Score:-3.86|P-value:5.65E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YER097W(YER097W_d)|FD-Score:3.85|P-value:5.94E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL109W(YGL109W_d)|FD-Score:4.48|P-value:3.70E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized gene YGL108C Gene:YGR051C(YGR051C_d)|FD-Score:-3.67|P-value:1.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene Gene:YKL133C(YKL133C_p)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YOR072W(YOR072W_d)|FD-Score:5.21|P-value:9.32E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR139C(YOR139C_d)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPR084W(YPR084W_p)|FD-Score:4.48|P-value:3.81E-6||SGD DESC:Putative protein of unknown function Gene:ABM1(YJR108W)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Protein of unknown function, required for normal microtubule organization Gene:ADE12(YNL220W)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:AHC2(YCR082W)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:AIM4(YBR194W)|FD-Score:4.27|P-value:9.91E-6||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:ATG27(YJL178C)|FD-Score:5.57|P-value:1.27E-8||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:BCH2(YKR027W)|FD-Score:3.72|P-value:9.96E-5||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BRE1(YDL074C)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BRE2(YLR015W)|FD-Score:-3.11|P-value:9.27E-4||SGD DESC:Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L Gene:CIN5(YOR028C)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication Gene:CNE1(YAL058W)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast Gene:DPP1(YDR284C)|FD-Score:5.22|P-value:8.84E-8||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:DSS1(YMR287C)|FD-Score:3.95|P-value:3.83E-5||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:GET3(YDL100C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GEX2(YKR106W)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GLC8(YMR311C)|FD-Score:-3.4|P-value:3.32E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GYP8(YFL027C)|FD-Score:-4.14|P-value:1.77E-5||SGD DESC:GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport Gene:HDA3(YPR179C)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p Gene:HST1(YOL068C)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:ICL2(YPR006C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol Gene:ISC1(YER019W)|FD-Score:4.32|P-value:7.66E-6||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:KES1(YPL145C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:LAA1(YJL207C)|FD-Score:-4.08|P-value:2.21E-5||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:MAG1(YER142C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress Gene:MAM33(YIL070C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R Gene:MDG1(YNL173C)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MET32(YDR253C)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MNE1(YOR350C)|FD-Score:3.92|P-value:4.48E-5||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MRF1(YGL143C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MSI1(YBR195C)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:NUM1(YDR150W)|FD-Score:5.7|P-value:5.98E-9||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:PCS60(YBR222C)|FD-Score:-4.05|P-value:2.56E-5||SGD DESC:Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase Gene:PET309(YLR067C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PRE9(YGR135W)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform Gene:PSY3(YLR376C)|FD-Score:3.77|P-value:8.06E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:PUT4(YOR348C)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells Gene:PYC1(YGL062W)|FD-Score:4.41|P-value:5.13E-6||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:RAD16(YBR114W)|FD-Score:5.99|P-value:1.03E-9||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RAD28(YDR030C)|FD-Score:5.13|P-value:1.47E-7||SGD DESC:Protein involved in DNA repair, related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair Gene:RFX1(YLR176C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RGC1(YPR115W)|FD-Score:4.56|P-value:2.60E-6||SGD DESC:Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication Gene:RPL13A(YDL082W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:SFM1(YOR021C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism Gene:SGA1(YIL099W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Intracellular sporulation-specific glucoamylase involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation Gene:SIP3(YNL257C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SIZ1(YDR409W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:TDA11(YHR159W_p)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TIF4632(YGL049C)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication Gene:VMA7(YGR020C)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:YAL067W-A(YAL067W-A_p)|FD-Score:-4.3|P-value:8.44E-6||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCH1(YGR203W)|FD-Score:3.89|P-value:4.92E-5||SGD DESC:Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YDR179W-A(YDR179W-A_p)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Putative protein of unknown function Gene:YDR209C(YDR209C_d)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W. Gene:YER053C-A(YER053C-A_p)|FD-Score:-3.86|P-value:5.65E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YER097W(YER097W_d)|FD-Score:3.85|P-value:5.94E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL109W(YGL109W_d)|FD-Score:4.48|P-value:3.70E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized gene YGL108C Gene:YGR051C(YGR051C_d)|FD-Score:-3.67|P-value:1.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene Gene:YKL133C(YKL133C_p)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YOR072W(YOR072W_d)|FD-Score:5.21|P-value:9.32E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR139C(YOR139C_d)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPR084W(YPR084W_p)|FD-Score:4.48|P-value:3.81E-6||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL163W_d6.417.19E-111.14YDL163W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase
YGL061C5.276.70E-80.45DUO1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YIL004C4.836.99E-70.72BET1Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins
YGL092W4.102.07E-50.18NUP145Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98
YLR197W3.924.44E-50.35NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YER006W3.571.76E-40.02NUG1GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus
YOR117W3.551.93E-40.23RPT5One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
YGR074W3.324.58E-40.05SMD1Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress
YBL004W3.275.41E-40.12UTP20Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
YHR036W3.158.29E-40.03BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
YNL113W3.129.04E-40.03RPC19RNA polymerase subunit AC19, common to RNA polymerases I and III
YBR038W3.099.84E-40.03CHS2Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis
YER009W3.060.001100.02NTF2Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport
YCL031C3.040.001190.01RRP7Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress
YBR079C3.030.001220.03RPG1eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR114W5.991.03E-9RAD16Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex
YDR150W5.705.98E-9NUM1Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex
YJL178C5.571.27E-8ATG27Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site
YDR284C5.228.84E-8DPP1Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism
YOR072W_d5.219.32E-8YOR072W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive
YDR030C5.131.47E-7RAD28Protein involved in DNA repair, related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair
YPR115W4.562.60E-6RGC1Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication
YKL133C_p4.552.74E-6YKL133C_pPutative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype)
YGL109W_d4.483.70E-6YGL109W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized gene YGL108C
YPR084W_p4.483.81E-6YPR084W_pPutative protein of unknown function
YGL062W4.415.13E-6PYC1Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication
YER019W4.327.66E-6ISC1Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance
YBR194W4.279.91E-6AIM4Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress
YPR179C4.261.04E-5HDA3Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p
YGL049C4.072.31E-5TIF4632Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication

GO enrichment analysis for SGTC_532
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0612.68E-6SGTC_30809117754 49.5 μMChembridge (Drug-like library)95501910.0786517
0.0603.54E-6SGTC_534k292-0788 63.4 μMChemDiv (Drug-like library)41463170.155844mitochondrial processes
0.0552.16E-5SGTC_15372',6'-dihydroxyflavanone 78.0 μMTimTec (Pure natural product library)51067870.1
0.0482.04E-4SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.0769231PDR1
0.0482.04E-4SGTC_32159129942 49.5 μMChembridge (Drug-like library)176829440.137931
0.0482.44E-4SGTC_6800417-1641 114.0 μMChemDiv (Drug-like library)28359900.0804598RPP1 & pyrimidine depletion
0.0463.99E-4SGTC_1887st057648 70.8 μMTimTec (Natural product derivative library)6886720.0864198
0.0464.19E-4SGTC_171k064-0041 9.7 μMChemDiv (Drug-like library)533250.0821918heme requiring
0.0464.16E-4SGTC_31799107801 49.5 μMChembridge (Drug-like library)48182590.142857
0.0454.77E-4SGTC_2726cimetidine 79.3 μMMiscellaneous27560.0674157
0.0455.41E-4SGTC_1864st057645 30.4 μMTimTec (Natural product derivative library)6886660.116883amide catabolism
0.0446.81E-4SGTC_31519099821 49.5 μMChembridge (Drug-like library)171741890.122222
0.0447.45E-4SGTC_9302810-3379 265.0 μMChemDiv (Drug-like library)10157640.105263
0.0430.00100SGTC_1038k292-0204 154.0 μMChemDiv (Drug-like library)44542120.232877
0.0439.91E-4SGTC_22199098279 200.0 μMChembridge (Fragment library)9023490.0933333

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_13161272-005065.9 μM0.4736847340392ChemDiv (Drug-like library)251.283222.02213
SGTC_6660272-0154136 μM0.3823536870312ChemDiv (Drug-like library)276.357342.41523cell wall
SGTC_13541498-168181 μM0.3815795754243ChemDiv (Drug-like library)493.309483.11335
SGTC_544k015-002052.4 μM0.338713008304ChemDiv (Drug-like library)316.14943.20802
SGTC_3021094-006318.9 μM0.3243245440289ChemDiv (Drug-like library)374.188782.47624
SGTC_1456k015-001213 μM0.3230772989616ChemDiv (Drug-like library)363.149873.03802
SGTC_9143820-6779408 μM0.3098592893481ChemDiv (Drug-like library)392.450884.69725
SGTC_1139k015-002446.9 μM0.3088242766652ChemDiv (Drug-like library)305.251313.40205PDR1
SGTC_20535265679138 μM0.287879764595Chembridge (Fragment library)233.286242.55113
SGTC_1280906-298113.37 μM0.2876716740226ChemDiv (Drug-like library)296.343682.0124