k292-0788

3-(3-methylbutyl)-2-sulfanylidene-1H-thieno[3,2-d]pyrimidin-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_534
Screen concentration 63.4 μM
Source ChemDiv (Drug-like library)
PubChem CID 4146317
SMILES CC(C)CCN1C(=O)C2=C(C=CS2)NC1=S
Standardized SMILES CC(C)CCN1C(=S)Nc2ccsc2C1=O
Molecular weight 254.3717
ALogP 3.68
H-bond donor count 1
H-bond acceptor count 3
Response signature mitochondrial processes

Pool Growth Kinetics
% growth inhibition (Het. pool) 34.57
% growth inhibition (Hom. pool) 13.17


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4146317
Download HIP data (tab-delimited text)  (excel)
Gene:BCP1(YDR361C)|FD-Score:5.24|P-value:8.17E-8|Clearance:0.58||SGD DESC:Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport Gene:CCT4(YDL143W)|FD-Score:3.18|P-value:7.44E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CFT1(YDR301W)|FD-Score:6.95|P-value:1.86E-12|Clearance:1.29||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:DAD2(YKR083C)|FD-Score:-3.33|P-value:4.41E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DFR1(YOR236W)|FD-Score:4.05|P-value:2.52E-5|Clearance:0.09||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:FHL1(YPR104C)|FD-Score:3.51|P-value:2.20E-4|Clearance:0.06||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:GLC7(YER133W)|FD-Score:4.24|P-value:1.11E-5|Clearance:0.01||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI18(YBR004C)|FD-Score:3.17|P-value:7.62E-4|Clearance:0.01||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:KEG1(YFR042W)|FD-Score:3.41|P-value:3.29E-4|Clearance:0.23||SGD DESC:Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability Gene:LSM2(YBL026W)|FD-Score:3.16|P-value:8.01E-4|Clearance:0.03||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MSL5(YLR116W)|FD-Score:-4.1|P-value:2.07E-5|Clearance:0||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:NOP56(YLR197W)|FD-Score:4.2|P-value:1.31E-5|Clearance:0.15||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PGA3(YML125C)|FD-Score:3.17|P-value:7.56E-4|Clearance:0||SGD DESC:Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication Gene:RER2(YBR002C)|FD-Score:3.91|P-value:4.66E-5|Clearance:0.05||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPC19(YNL113W)|FD-Score:4.23|P-value:1.18E-5|Clearance:0.02||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RRP15(YPR143W)|FD-Score:3.97|P-value:3.65E-5|Clearance:0.06||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RRP4(YHR069C)|FD-Score:3.86|P-value:5.76E-5|Clearance:0.34||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RSC8(YFR037C)|FD-Score:5.66|P-value:7.72E-9|Clearance:0.42||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:4.52|P-value:3.08E-6|Clearance:0.28||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:RTP1(YMR185W_p)|FD-Score:3.12|P-value:9.01E-4|Clearance:0.04||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SUP35(YDR172W)|FD-Score:4.66|P-value:1.61E-6|Clearance:0.09||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TCP1(YDR212W)|FD-Score:3.45|P-value:2.79E-4|Clearance:0.04||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TEM1(YML064C)|FD-Score:3.13|P-value:8.89E-4|Clearance:0||SGD DESC:GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis Gene:YOR218C(YOR218C_d)|FD-Score:4.56|P-value:2.53E-6|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W Gene:BCP1(YDR361C)|FD-Score:5.24|P-value:8.17E-8|Clearance:0.58||SGD DESC:Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport Gene:CCT4(YDL143W)|FD-Score:3.18|P-value:7.44E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CFT1(YDR301W)|FD-Score:6.95|P-value:1.86E-12|Clearance:1.29||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:DAD2(YKR083C)|FD-Score:-3.33|P-value:4.41E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DFR1(YOR236W)|FD-Score:4.05|P-value:2.52E-5|Clearance:0.09||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:FHL1(YPR104C)|FD-Score:3.51|P-value:2.20E-4|Clearance:0.06||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:GLC7(YER133W)|FD-Score:4.24|P-value:1.11E-5|Clearance:0.01||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI18(YBR004C)|FD-Score:3.17|P-value:7.62E-4|Clearance:0.01||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:KEG1(YFR042W)|FD-Score:3.41|P-value:3.29E-4|Clearance:0.23||SGD DESC:Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability Gene:LSM2(YBL026W)|FD-Score:3.16|P-value:8.01E-4|Clearance:0.03||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MSL5(YLR116W)|FD-Score:-4.1|P-value:2.07E-5|Clearance:0||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:NOP56(YLR197W)|FD-Score:4.2|P-value:1.31E-5|Clearance:0.15||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PGA3(YML125C)|FD-Score:3.17|P-value:7.56E-4|Clearance:0||SGD DESC:Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication Gene:RER2(YBR002C)|FD-Score:3.91|P-value:4.66E-5|Clearance:0.05||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPC19(YNL113W)|FD-Score:4.23|P-value:1.18E-5|Clearance:0.02||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RRP15(YPR143W)|FD-Score:3.97|P-value:3.65E-5|Clearance:0.06||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RRP4(YHR069C)|FD-Score:3.86|P-value:5.76E-5|Clearance:0.34||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RSC8(YFR037C)|FD-Score:5.66|P-value:7.72E-9|Clearance:0.42||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:4.52|P-value:3.08E-6|Clearance:0.28||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:RTP1(YMR185W_p)|FD-Score:3.12|P-value:9.01E-4|Clearance:0.04||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SUP35(YDR172W)|FD-Score:4.66|P-value:1.61E-6|Clearance:0.09||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TCP1(YDR212W)|FD-Score:3.45|P-value:2.79E-4|Clearance:0.04||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TEM1(YML064C)|FD-Score:3.13|P-value:8.89E-4|Clearance:0||SGD DESC:GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis Gene:YOR218C(YOR218C_d)|FD-Score:4.56|P-value:2.53E-6|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4146317
Download HOP data (tab-delimited text)  (excel)
Gene:AHC2(YCR082W)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:APA1(YCL050C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication Gene:BUD16(YEL029C)|FD-Score:-3.17|P-value:7.51E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:BUD3(YCL014W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:CAF4(YKR036C)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:WD40 repeat-containing protein associated with the CCR4-NOT complex; interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; CAF4 has a paralog, MDV1, that arose from the whole genome duplication Gene:CHL1(YPL008W)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome Gene:DAL81(YIR023W)|FD-Score:5.99|P-value:1.04E-9||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DSS4(YPR017C)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:ECM38(YLR299W)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:Gamma-glutamyltranspeptidase, major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation Gene:ECM5(YMR176W)|FD-Score:4.39|P-value:5.74E-6||SGD DESC:Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress Gene:FZO1(YBR179C)|FD-Score:5.11|P-value:1.63E-7||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GCN2(YDR283C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GDE1(YPL110C)|FD-Score:6.37|P-value:9.24E-11||SGD DESC:Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes Gene:GPB2(YAL056W)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:HOC1(YJR075W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele Gene:HOM3(YER052C)|FD-Score:4.65|P-value:1.67E-6||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:KEL3(YPL263C)|FD-Score:4.36|P-value:6.55E-6||SGD DESC:Cytoplasmic protein of unknown function Gene:LCB5(YLR260W)|FD-Score:3.26|P-value:5.47E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LEU4(YNL104C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication Gene:MAL12(YGR292W)|FD-Score:4.92|P-value:4.31E-7||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MCM21(YDR318W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MDY2(YOL111C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MHP1(YJL042W)|FD-Score:4.57|P-value:2.38E-6||SGD DESC:Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins Gene:MLF3(YNL074C)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Serine-rich protein of unknown function, predicted to be palmitoylated; overproduction suppresses the growth inhibition caused by exposure to the immunosuppressant leflunomide Gene:MMS1(YPR164W)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MRK1(YDL079C)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:PAU17(YLL025W)|FD-Score:4.94|P-value:3.87E-7||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene Gene:PDR8(YLR266C)|FD-Score:3.76|P-value:8.48E-5||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PIM1(YBL022C)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:POS5(YPL188W)|FD-Score:3.77|P-value:8.22E-5||SGD DESC:Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress Gene:QRI5(YLR204W)|FD-Score:-3.19|P-value:7.13E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAD16(YBR114W)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:REV7(YIL139C)|FD-Score:4.98|P-value:3.13E-7||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RGL1(YPL066W)|FD-Score:3.72|P-value:9.91E-5||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RKR1(YMR247C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice Gene:RPA34(YJL148W)|FD-Score:4.78|P-value:8.58E-7||SGD DESC:RNA polymerase I subunit A34.5 Gene:RPS8A(YBL072C)|FD-Score:-3.93|P-value:4.28E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RRT14(YIL127C_p)|FD-Score:6.2|P-value:2.90E-10||SGD DESC:Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:SCT1(YBL011W)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SCW10(YMR305C)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p Gene:SPG4(YMR107W)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SSD1(YDR293C)|FD-Score:3.89|P-value:4.98E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:TAE2(YPL009C)|FD-Score:4.67|P-value:1.54E-6||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:TIF1(YKR059W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication Gene:TRP3(YKL211C)|FD-Score:4.49|P-value:3.58E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VMA7(YGR020C)|FD-Score:8.43|P-value:1.73E-17||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:XPT1(YJR133W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Xanthine-guanine phosphoribosyl transferase, required for xanthine utilization and for optimal utilization of guanine Gene:YAL067W-A(YAL067W-A_p)|FD-Score:-5.19|P-value:1.06E-7||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBL039W-B(YBL039W-B_p)|FD-Score:-5.72|P-value:5.22E-9||SGD DESC:Putative protein of unknown function Gene:YBL083C(YBL083C_d)|FD-Score:4.48|P-value:3.65E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YCF1(YDR135C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR Gene:YDR008C(YDR008C_d)|FD-Score:4.85|P-value:6.13E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR109C(YDR109C_p)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Putative kinase Gene:YDR179W-A(YDR179W-A_p)|FD-Score:3.72|P-value:9.86E-5||SGD DESC:Putative protein of unknown function Gene:YDR387C(YDR387C_p)|FD-Score:5.24|P-value:8.08E-8||SGD DESC:Putative transporter, member of the sugar porter family; YDR387C is not an essential gene Gene:YDR476C(YDR476C_p)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YDR541C(YDR541C_p)|FD-Score:4.53|P-value:2.97E-6||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YEL057C(YEL057C_p)|FD-Score:4.32|P-value:7.83E-6||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YER097W(YER097W_d)|FD-Score:4.9|P-value:4.67E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFR039C(YFR039C_p)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces Gene:YGL015C(YGL015C_p)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:Putative protein of unknown function; null mutants accumulate cargo in the Golgi Gene:YHI9(YHR029C)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YHR022C(YHR022C_p)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Putative protein of unknown function; YHR022C is not an essential gene Gene:YHR035W(YHR035W_p)|FD-Score:4.47|P-value:3.99E-6||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YLL053C(YLL053C_p)|FD-Score:3.76|P-value:8.57E-5||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YMC2(YBR104W)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC2 has a paralog, YMC1, that arose from the whole genome duplication Gene:YMR175W-A(YMR175W-A_p)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Putative protein of unknown function Gene:YNL115C(YNL115C_p)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene Gene:YNL211C(YNL211C_p)|FD-Score:-3.72|P-value:9.99E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YOL131W(YOL131W_p)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Putative protein of unknown function Gene:YOR024W(YOR024W_d)|FD-Score:4.63|P-value:1.80E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR152C(YOR152C_p)|FD-Score:3.11|P-value:9.24E-4||SGD DESC:Putative protein of unknown function; YOR152C is not an essential gene Gene:YOR342C(YOR342C_p)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication Gene:YPL068C(YPL068C_p)|FD-Score:-3.17|P-value:7.55E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YRR1(YOR162C)|FD-Score:-3.81|P-value:6.97E-5||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:AHC2(YCR082W)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:APA1(YCL050C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication Gene:BUD16(YEL029C)|FD-Score:-3.17|P-value:7.51E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:BUD3(YCL014W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:CAF4(YKR036C)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:WD40 repeat-containing protein associated with the CCR4-NOT complex; interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; CAF4 has a paralog, MDV1, that arose from the whole genome duplication Gene:CHL1(YPL008W)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome Gene:DAL81(YIR023W)|FD-Score:5.99|P-value:1.04E-9||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DSS4(YPR017C)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:ECM38(YLR299W)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:Gamma-glutamyltranspeptidase, major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation Gene:ECM5(YMR176W)|FD-Score:4.39|P-value:5.74E-6||SGD DESC:Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress Gene:FZO1(YBR179C)|FD-Score:5.11|P-value:1.63E-7||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GCN2(YDR283C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GDE1(YPL110C)|FD-Score:6.37|P-value:9.24E-11||SGD DESC:Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes Gene:GPB2(YAL056W)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:HOC1(YJR075W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele Gene:HOM3(YER052C)|FD-Score:4.65|P-value:1.67E-6||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:KEL3(YPL263C)|FD-Score:4.36|P-value:6.55E-6||SGD DESC:Cytoplasmic protein of unknown function Gene:LCB5(YLR260W)|FD-Score:3.26|P-value:5.47E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LEU4(YNL104C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication Gene:MAL12(YGR292W)|FD-Score:4.92|P-value:4.31E-7||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MCM21(YDR318W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MDY2(YOL111C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MHP1(YJL042W)|FD-Score:4.57|P-value:2.38E-6||SGD DESC:Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins Gene:MLF3(YNL074C)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Serine-rich protein of unknown function, predicted to be palmitoylated; overproduction suppresses the growth inhibition caused by exposure to the immunosuppressant leflunomide Gene:MMS1(YPR164W)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MRK1(YDL079C)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:PAU17(YLL025W)|FD-Score:4.94|P-value:3.87E-7||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene Gene:PDR8(YLR266C)|FD-Score:3.76|P-value:8.48E-5||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PIM1(YBL022C)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:POS5(YPL188W)|FD-Score:3.77|P-value:8.22E-5||SGD DESC:Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress Gene:QRI5(YLR204W)|FD-Score:-3.19|P-value:7.13E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAD16(YBR114W)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:REV7(YIL139C)|FD-Score:4.98|P-value:3.13E-7||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RGL1(YPL066W)|FD-Score:3.72|P-value:9.91E-5||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RKR1(YMR247C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice Gene:RPA34(YJL148W)|FD-Score:4.78|P-value:8.58E-7||SGD DESC:RNA polymerase I subunit A34.5 Gene:RPS8A(YBL072C)|FD-Score:-3.93|P-value:4.28E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RRT14(YIL127C_p)|FD-Score:6.2|P-value:2.90E-10||SGD DESC:Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:SCT1(YBL011W)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SCW10(YMR305C)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p Gene:SPG4(YMR107W)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SSD1(YDR293C)|FD-Score:3.89|P-value:4.98E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:TAE2(YPL009C)|FD-Score:4.67|P-value:1.54E-6||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:TIF1(YKR059W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication Gene:TRP3(YKL211C)|FD-Score:4.49|P-value:3.58E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VMA7(YGR020C)|FD-Score:8.43|P-value:1.73E-17||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:XPT1(YJR133W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Xanthine-guanine phosphoribosyl transferase, required for xanthine utilization and for optimal utilization of guanine Gene:YAL067W-A(YAL067W-A_p)|FD-Score:-5.19|P-value:1.06E-7||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBL039W-B(YBL039W-B_p)|FD-Score:-5.72|P-value:5.22E-9||SGD DESC:Putative protein of unknown function Gene:YBL083C(YBL083C_d)|FD-Score:4.48|P-value:3.65E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YCF1(YDR135C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR Gene:YDR008C(YDR008C_d)|FD-Score:4.85|P-value:6.13E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR109C(YDR109C_p)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Putative kinase Gene:YDR179W-A(YDR179W-A_p)|FD-Score:3.72|P-value:9.86E-5||SGD DESC:Putative protein of unknown function Gene:YDR387C(YDR387C_p)|FD-Score:5.24|P-value:8.08E-8||SGD DESC:Putative transporter, member of the sugar porter family; YDR387C is not an essential gene Gene:YDR476C(YDR476C_p)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YDR541C(YDR541C_p)|FD-Score:4.53|P-value:2.97E-6||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YEL057C(YEL057C_p)|FD-Score:4.32|P-value:7.83E-6||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YER097W(YER097W_d)|FD-Score:4.9|P-value:4.67E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFR039C(YFR039C_p)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces Gene:YGL015C(YGL015C_p)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:Putative protein of unknown function; null mutants accumulate cargo in the Golgi Gene:YHI9(YHR029C)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YHR022C(YHR022C_p)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Putative protein of unknown function; YHR022C is not an essential gene Gene:YHR035W(YHR035W_p)|FD-Score:4.47|P-value:3.99E-6||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YLL053C(YLL053C_p)|FD-Score:3.76|P-value:8.57E-5||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YMC2(YBR104W)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC2 has a paralog, YMC1, that arose from the whole genome duplication Gene:YMR175W-A(YMR175W-A_p)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Putative protein of unknown function Gene:YNL115C(YNL115C_p)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene Gene:YNL211C(YNL211C_p)|FD-Score:-3.72|P-value:9.99E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YOL131W(YOL131W_p)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Putative protein of unknown function Gene:YOR024W(YOR024W_d)|FD-Score:4.63|P-value:1.80E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR152C(YOR152C_p)|FD-Score:3.11|P-value:9.24E-4||SGD DESC:Putative protein of unknown function; YOR152C is not an essential gene Gene:YOR342C(YOR342C_p)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication Gene:YPL068C(YPL068C_p)|FD-Score:-3.17|P-value:7.55E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YRR1(YOR162C)|FD-Score:-3.81|P-value:6.97E-5||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR301W6.951.86E-121.29CFT1RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF
YFR037C5.667.72E-90.42RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YDR361C5.248.17E-80.58BCP1Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport
YDR172W4.661.61E-60.09SUP35Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
YOR218C_d4.562.53E-60.04YOR218C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W
YML127W4.523.08E-60.28RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YER133W4.241.11E-50.01GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YNL113W4.231.18E-50.02RPC19RNA polymerase subunit AC19, common to RNA polymerases I and III
YLR197W4.201.31E-50.15NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YOR236W4.052.52E-50.09DFR1Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
YPR143W3.973.65E-50.06RRP15Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
YBR002C3.914.66E-50.05RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YHR069C3.865.76E-50.34RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YPR104C3.512.20E-40.06FHL1Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
YDR212W3.452.79E-40.04TCP1Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR020C8.431.73E-17VMA7Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane
YPL110C6.379.24E-11GDE1Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes
YIL127C_p6.202.90E-10RRT14_pPutative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis
YIR023W5.991.04E-9DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YDR387C_p5.248.08E-8YDR387C_pPutative transporter, member of the sugar porter family; YDR387C is not an essential gene
YBR179C5.111.63E-7FZO1Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system
YIL139C4.983.13E-7REV7Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair
YLL025W4.943.87E-7PAU17Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene
YGR292W4.924.31E-7MAL12Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose
YER097W_d4.904.67E-7YER097W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDR008C_d4.856.13E-7YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YJL148W4.788.58E-7RPA34RNA polymerase I subunit A34.5
YGL015C_p4.751.02E-6YGL015C_pPutative protein of unknown function; null mutants accumulate cargo in the Golgi
YPL009C4.671.54E-6TAE2Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YER052C4.651.67E-6HOM3Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis

GO enrichment analysis for SGTC_534
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1287.78E-23SGTC_2683piperine 63.0 μMTimTec (Pure natural product library)6380240.0704225
0.1189.94E-20SGTC_5382922-0838 53.3 μMChemDiv (Drug-like library)7770390.0447761Golgi
0.1171.53E-19SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.0625
0.1159.92E-19SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.0806452
0.1141.21E-18SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.123077
0.1141.43E-18SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.097561
0.1141.63E-18SGTC_10583448-5425 97.3 μMChemDiv (Drug-like library)72797050.112903
0.1094.75E-17SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.0833333
0.1081.03E-16SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.0769231
0.1054.58E-16SGTC_14944358-1428 177.0 μMChemDiv (Drug-like library)15645710.0609756
0.1041.22E-15SGTC_486niguldipine 82.0 μMMiscellaneous12360.107527amide catabolism
0.1031.98E-15SGTC_6033794-0023 45.9 μMChemDiv (Drug-like library)38022600.121212
0.1023.59E-15SGTC_14780453-0656 35.7 μMChemDiv (Drug-like library)52584960.0724638
0.1024.81E-15SGTC_2409alverine citrate 64.0 μMMiscellaneous217180.0909091
0.1025.30E-15SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.160714TRP & mitochondrial translation

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_711k292-0785179 μM0.6976744146315ChemDiv (Drug-like library)240.345083.42413DNA intercalators
SGTC_1038k292-0204154 μM0.6304354454212ChemDiv (Drug-like library)288.38788413
SGTC_326k292-081217.5 μM0.2465753579357ChemDiv (Drug-like library)390.476524.2927
SGTC_21055350118200 μM0.2456142133313Chembridge (Fragment library)249.3322.69913
SGTC_2065522159634.26 μM0.222222788581Chembridge (Fragment library)238.286141.71724
SGTC_2305744352269.23 μM0.2131155415886Chembridge (Fragment library)279.2901031.51625
SGTC_305-(2-thienylidene)-Rhodanine28.7 μM0.2105261241132ChemDiv (Drug-like library)227.32642.79414
SGTC_2032518511225.99 μM0.25718189Chembridge (Fragment library)241.286781.54415
SGTC_2448518511227.17 μM0.25718189Chembridge (Fragment library)241.286781.54415
SGTC_24465162541200 μM0.21549321Miscellaneous267.367123.77104
SGTC_2451585622345.7 μM0.196972179896Miscellaneous269.339942.31105