1334-0398

2-[(2Z)-2-[(4-methoxyphenyl)methylidene]hydrazinyl]benzoic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_537
Screen concentration 209.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 5846986
SMILES COC1=CC=C(C=C1)C=NNC2=CC=CC=C2C(=O)O
Standardized SMILES COc1ccc(C=NNc2ccccc2C(=O)O)cc1
Molecular weight 270.2833
ALogP 3.06
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 28
% growth inhibition (Hom. pool) 15.55


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5846986
Download HIP data (tab-delimited text)  (excel)
Gene:CAB3(YKL088W)|FD-Score:3.28|P-value:5.18E-4|Clearance:0.13||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:DUO1(YGL061C)|FD-Score:3.75|P-value:8.85E-5|Clearance:0.06||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:GCD11(YER025W)|FD-Score:3.76|P-value:8.41E-5|Clearance:0.01||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GPI13(YLL031C)|FD-Score:-3.1|P-value:9.59E-4|Clearance:0||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:KAE1(YKR038C)|FD-Score:3.83|P-value:6.50E-5|Clearance:0||SGD DESC:Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:NIP1(YMR309C)|FD-Score:3.69|P-value:1.10E-4|Clearance:0.1||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:PKC1(YBL105C)|FD-Score:-3.16|P-value:7.86E-4|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:POL2(YNL262W)|FD-Score:3.83|P-value:6.43E-5|Clearance:0||SGD DESC:Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p Gene:PZF1(YPR186C)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.02||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RPL30(YGL030W)|FD-Score:4.87|P-value:5.61E-7|Clearance:0.68||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RPP1(YHR062C)|FD-Score:3.11|P-value:9.26E-4|Clearance:0.01||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS13(YDR064W)|FD-Score:3.84|P-value:6.24E-5|Clearance:0.01||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:SEC13(YLR208W)|FD-Score:3.82|P-value:6.63E-5|Clearance:0.06||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SUI3(YPL237W)|FD-Score:3.59|P-value:1.64E-4|Clearance:0.03||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TOP2(YNL088W)|FD-Score:3.15|P-value:8.21E-4|Clearance:0.04||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YKL083W(YKL083W_d)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.26||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YLR076C(YLR076C_d)|FD-Score:4.19|P-value:1.41E-5|Clearance:0.35||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YLR198C(YLR198C_d)|FD-Score:3.11|P-value:9.51E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:YPL238C(YPL238C_d)|FD-Score:5.47|P-value:2.19E-8|Clearance:0.68||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:CAB3(YKL088W)|FD-Score:3.28|P-value:5.18E-4|Clearance:0.13||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:DUO1(YGL061C)|FD-Score:3.75|P-value:8.85E-5|Clearance:0.06||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:GCD11(YER025W)|FD-Score:3.76|P-value:8.41E-5|Clearance:0.01||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GPI13(YLL031C)|FD-Score:-3.1|P-value:9.59E-4|Clearance:0||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:KAE1(YKR038C)|FD-Score:3.83|P-value:6.50E-5|Clearance:0||SGD DESC:Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:NIP1(YMR309C)|FD-Score:3.69|P-value:1.10E-4|Clearance:0.1||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:PKC1(YBL105C)|FD-Score:-3.16|P-value:7.86E-4|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:POL2(YNL262W)|FD-Score:3.83|P-value:6.43E-5|Clearance:0||SGD DESC:Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p Gene:PZF1(YPR186C)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.02||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RPL30(YGL030W)|FD-Score:4.87|P-value:5.61E-7|Clearance:0.68||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RPP1(YHR062C)|FD-Score:3.11|P-value:9.26E-4|Clearance:0.01||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS13(YDR064W)|FD-Score:3.84|P-value:6.24E-5|Clearance:0.01||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:SEC13(YLR208W)|FD-Score:3.82|P-value:6.63E-5|Clearance:0.06||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SUI3(YPL237W)|FD-Score:3.59|P-value:1.64E-4|Clearance:0.03||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TOP2(YNL088W)|FD-Score:3.15|P-value:8.21E-4|Clearance:0.04||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YKL083W(YKL083W_d)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.26||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YLR076C(YLR076C_d)|FD-Score:4.19|P-value:1.41E-5|Clearance:0.35||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YLR198C(YLR198C_d)|FD-Score:3.11|P-value:9.51E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:YPL238C(YPL238C_d)|FD-Score:5.47|P-value:2.19E-8|Clearance:0.68||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5846986
Download HOP data (tab-delimited text)  (excel)
Gene:ACS1(YAL054C)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:ADH6(YMR318C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:ADY2(YCR010C)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:ADY4(YLR227C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Structural component of the meiotic outer plaque, which is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane Gene:AGE2(YIL044C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:APM3(YBR288C)|FD-Score:-3.82|P-value:6.71E-5||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:BEM3(YPL115C)|FD-Score:5.39|P-value:3.49E-8||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:CLN3(YAL040C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:COG6(YNL041C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTF4(YPR135W)|FD-Score:3.88|P-value:5.21E-5||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:DAL81(YIR023W)|FD-Score:5.19|P-value:1.05E-7||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBP3(YGL078C)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype Gene:DGR2(YKL121W_p)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:EAF6(YJR082C)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:ENV7(YPL236C)|FD-Score:5.01|P-value:2.76E-7||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:ERV14(YGL054C)|FD-Score:8.89|P-value:2.94E-19||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FKH1(YIL131C)|FD-Score:4.05|P-value:2.52E-5||SGD DESC:Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication Gene:FMP30(YPL103C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D Gene:FRE4(YNR060W)|FD-Score:3.84|P-value:6.13E-5||SGD DESC:Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FSH2(YMR222C)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 Gene:FUM1(YPL262W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GDH2(YDL215C)|FD-Score:3.86|P-value:5.69E-5||SGD DESC:NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels Gene:GET2(YER083C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:HCH1(YNL281W)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:ILM1(YJR118C)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IML2(YJL082W)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication Gene:IRC15(YPL017C)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:ISC1(YER019W)|FD-Score:5.89|P-value:1.88E-9||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:LAP2(YNL045W)|FD-Score:3.96|P-value:3.78E-5||SGD DESC:Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:LEU4(YNL104C)|FD-Score:4.49|P-value:3.57E-6||SGD DESC:Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication Gene:MAC1(YMR021C)|FD-Score:-5.76|P-value:4.19E-9||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MCT1(YOR221C)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MHF1(YOL086W-A)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 Gene:MMM1(YLL006W)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MND2(YIR025W)|FD-Score:-3.24|P-value:5.97E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:NGL1(YOL042W)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:OPY1(YBR129C)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Protein of unknown function, overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PEX17(YNL214W)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis Gene:PFK26(YIL107C)|FD-Score:-3.1|P-value:9.64E-4||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:RAD59(YDL059C)|FD-Score:-3.45|P-value:2.75E-4||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RNR3(YIL066C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Minor isoform of the large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits Gene:ROX1(YPR065W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RPL43A(YPR043W)|FD-Score:-3.11|P-value:9.29E-4||SGD DESC:Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:SBH1(YER087C-B)|FD-Score:5.12|P-value:1.52E-7||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SCT1(YBL011W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SEC22(YLR268W)|FD-Score:5.34|P-value:4.52E-8||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SHB17(YKR043C)|FD-Score:-3.8|P-value:7.11E-5||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SLX4(YLR135W)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Endonuclease involved in processing DNA; acts during recombination and repair; cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress Gene:SNF6(YHL025W)|FD-Score:-3.13|P-value:8.82E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SPT2(YER161C)|FD-Score:4.61|P-value:2.00E-6||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SSD1(YDR293C)|FD-Score:-3.3|P-value:4.83E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STE50(YCL032W)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SUL2(YLR092W)|FD-Score:4.33|P-value:7.62E-6||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:TDH3(YGR192C)|FD-Score:3.92|P-value:4.49E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication Gene:TMA16(YOR252W)|FD-Score:-3.1|P-value:9.64E-4||SGD DESC:Protein of unknown function that associates with ribosomes Gene:TOP1(YOL006C)|FD-Score:4.38|P-value:6.06E-6||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:TRF5(YNL299W)|FD-Score:3.85|P-value:5.92E-5||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:TSC3(YBR058C-A)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VPS63(YLR261C_d)|FD-Score:4.55|P-value:2.67E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:WSC4(YHL028W)|FD-Score:3.89|P-value:5.04E-5||SGD DESC:ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 Gene:XDJ1(YLR090W)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YAL067W-A(YAL067W-A_p)|FD-Score:6.79|P-value:5.60E-12||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBL008W-A(YBL008W-A_p)|FD-Score:-4.53|P-value:3.00E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBL039W-B(YBL039W-B_p)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Putative protein of unknown function Gene:YBR138C(YBR138C_p)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene Gene:YDR541C(YDR541C_p)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YGR122C-A(YGR122C-A_d)|FD-Score:-3.12|P-value:8.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W Gene:YIR020C(YIR020C_p)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YLR334C(YLR334C_d)|FD-Score:4.63|P-value:1.82E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here Gene:YLR358C(YLR358C_p)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YMR086C-A(YMR086C-A_d)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL235C(YNL235C_d)|FD-Score:4.4|P-value:5.36E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YNL324W(YNL324W_d)|FD-Score:3.75|P-value:8.96E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL131W(YOL131W_p)|FD-Score:-4.27|P-value:9.97E-6||SGD DESC:Putative protein of unknown function Gene:YOR020W-A(YOR020W-A_p)|FD-Score:3.31|P-value:4.75E-4||SGD DESC:Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPL080C(YPL080C_d)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YRR1(YOR162C)|FD-Score:15.7|P-value:9.86E-56||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ACS1(YAL054C)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:ADH6(YMR318C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:ADY2(YCR010C)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:ADY4(YLR227C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Structural component of the meiotic outer plaque, which is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane Gene:AGE2(YIL044C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:APM3(YBR288C)|FD-Score:-3.82|P-value:6.71E-5||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:BEM3(YPL115C)|FD-Score:5.39|P-value:3.49E-8||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:CLN3(YAL040C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:COG6(YNL041C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTF4(YPR135W)|FD-Score:3.88|P-value:5.21E-5||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:DAL81(YIR023W)|FD-Score:5.19|P-value:1.05E-7||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBP3(YGL078C)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype Gene:DGR2(YKL121W_p)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:EAF6(YJR082C)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:ENV7(YPL236C)|FD-Score:5.01|P-value:2.76E-7||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:ERV14(YGL054C)|FD-Score:8.89|P-value:2.94E-19||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FKH1(YIL131C)|FD-Score:4.05|P-value:2.52E-5||SGD DESC:Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication Gene:FMP30(YPL103C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D Gene:FRE4(YNR060W)|FD-Score:3.84|P-value:6.13E-5||SGD DESC:Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FSH2(YMR222C)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 Gene:FUM1(YPL262W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GDH2(YDL215C)|FD-Score:3.86|P-value:5.69E-5||SGD DESC:NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels Gene:GET2(YER083C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:HCH1(YNL281W)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:ILM1(YJR118C)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IML2(YJL082W)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication Gene:IRC15(YPL017C)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:ISC1(YER019W)|FD-Score:5.89|P-value:1.88E-9||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:LAP2(YNL045W)|FD-Score:3.96|P-value:3.78E-5||SGD DESC:Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:LEU4(YNL104C)|FD-Score:4.49|P-value:3.57E-6||SGD DESC:Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication Gene:MAC1(YMR021C)|FD-Score:-5.76|P-value:4.19E-9||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MCT1(YOR221C)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MHF1(YOL086W-A)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 Gene:MMM1(YLL006W)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MND2(YIR025W)|FD-Score:-3.24|P-value:5.97E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:NGL1(YOL042W)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:OPY1(YBR129C)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Protein of unknown function, overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PEX17(YNL214W)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis Gene:PFK26(YIL107C)|FD-Score:-3.1|P-value:9.64E-4||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:RAD59(YDL059C)|FD-Score:-3.45|P-value:2.75E-4||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RNR3(YIL066C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Minor isoform of the large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits Gene:ROX1(YPR065W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RPL43A(YPR043W)|FD-Score:-3.11|P-value:9.29E-4||SGD DESC:Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:SBH1(YER087C-B)|FD-Score:5.12|P-value:1.52E-7||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SCT1(YBL011W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SEC22(YLR268W)|FD-Score:5.34|P-value:4.52E-8||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SHB17(YKR043C)|FD-Score:-3.8|P-value:7.11E-5||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SLX4(YLR135W)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Endonuclease involved in processing DNA; acts during recombination and repair; cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress Gene:SNF6(YHL025W)|FD-Score:-3.13|P-value:8.82E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SPT2(YER161C)|FD-Score:4.61|P-value:2.00E-6||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SSD1(YDR293C)|FD-Score:-3.3|P-value:4.83E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STE50(YCL032W)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SUL2(YLR092W)|FD-Score:4.33|P-value:7.62E-6||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:TDH3(YGR192C)|FD-Score:3.92|P-value:4.49E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication Gene:TMA16(YOR252W)|FD-Score:-3.1|P-value:9.64E-4||SGD DESC:Protein of unknown function that associates with ribosomes Gene:TOP1(YOL006C)|FD-Score:4.38|P-value:6.06E-6||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:TRF5(YNL299W)|FD-Score:3.85|P-value:5.92E-5||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:TSC3(YBR058C-A)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VPS63(YLR261C_d)|FD-Score:4.55|P-value:2.67E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:WSC4(YHL028W)|FD-Score:3.89|P-value:5.04E-5||SGD DESC:ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 Gene:XDJ1(YLR090W)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YAL067W-A(YAL067W-A_p)|FD-Score:6.79|P-value:5.60E-12||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBL008W-A(YBL008W-A_p)|FD-Score:-4.53|P-value:3.00E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBL039W-B(YBL039W-B_p)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Putative protein of unknown function Gene:YBR138C(YBR138C_p)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene Gene:YDR541C(YDR541C_p)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YGR122C-A(YGR122C-A_d)|FD-Score:-3.12|P-value:8.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W Gene:YIR020C(YIR020C_p)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YLR334C(YLR334C_d)|FD-Score:4.63|P-value:1.82E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here Gene:YLR358C(YLR358C_p)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YMR086C-A(YMR086C-A_d)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL235C(YNL235C_d)|FD-Score:4.4|P-value:5.36E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YNL324W(YNL324W_d)|FD-Score:3.75|P-value:8.96E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL131W(YOL131W_p)|FD-Score:-4.27|P-value:9.97E-6||SGD DESC:Putative protein of unknown function Gene:YOR020W-A(YOR020W-A_p)|FD-Score:3.31|P-value:4.75E-4||SGD DESC:Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPL080C(YPL080C_d)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YRR1(YOR162C)|FD-Score:15.7|P-value:9.86E-56||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL238C_d5.472.19E-80.68YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YGL030W4.875.61E-70.68RPL30Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog
YLR076C_d4.191.41E-50.35YLR076C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10
YDR064W3.846.24E-50.01RPS13Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15
YNL262W3.836.43E-50.00POL2Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p
YKR038C3.836.50E-50.00KAE1Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription
YLR208W3.826.63E-50.06SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YER025W3.768.41E-50.01GCD11Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met
YGL061C3.758.85E-50.06DUO1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YMR309C3.691.10E-40.10NIP1eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection
YPL237W3.591.64E-40.03SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
YPR186C3.561.84E-40.02PZF1Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA
YKL083W_d3.542.00E-40.26YKL083W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
YKL088W3.285.18E-40.13CAB3Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC
YNL088W3.158.21E-40.04TOP2Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR162C15.709.86E-56YRR1Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication
YGL054C8.892.94E-19ERV14COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
YAL067W-A_p6.795.60E-12YAL067W-A_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YER019W5.891.88E-9ISC1Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance
YPL115C5.393.49E-8BEM3Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
YLR268W5.344.52E-8SEC22R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog
YIR023W5.191.05E-7DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YER087C-B5.121.52E-7SBH1Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p
YPL236C5.012.76E-7ENV7Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16)
YDR541C_p4.711.26E-6YDR541C_pPutative dihydrokaempferol 4-reductase
YLR334C_d4.631.82E-6YLR334C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here
YER161C4.612.00E-6SPT2Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins
YLR261C_d4.552.67E-6VPS63_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect
YNL104C4.493.57E-6LEU4Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication
YNL235C_d4.405.36E-6YNL235C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex

GO enrichment analysis for SGTC_537
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2124.25E-61SGTC_210849-0113 23.0 μMChemDiv (Drug-like library)15601560.114754
0.1554.96E-33SGTC_2615purpurogallin-4-carboxylic acid 100.0 μMMicrosource (Natural product library)2693150.131148redox potentiating
0.1087.19E-17SGTC_203k066-0004 30.9 μMChemDiv (Drug-like library)67954120.0972222RSC & ERG11
0.1056.51E-16SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.162162TSC3-RPN4
0.1033.16E-15SGTC_30833-0587 95.6 μMChemDiv (Drug-like library)53826440.254545redox potentiating
0.1017.01E-15SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.224138TSC3-RPN4
0.1001.69E-14SGTC_32379133362 49.5 μMChembridge (Drug-like library)438451370.125
0.0992.11E-14SGTC_9701315-0089 19.5 μMChemDiv (Drug-like library)68110830.222222RSC & ERG11
0.0976.75E-14SGTC_25732',4'-dihydroxychalcone 15.0 μMTimTec (Pure natural product library)53572180.264151
0.0979.49E-14SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0606061TSC3-RPN4
0.0961.48E-13SGTC_2902043-6722 48.1 μMChemDiv (Drug-like library)7737580.166667
0.0952.05E-13SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0298507TSC3-RPN4
0.0943.67E-13SGTC_22186965428 193.0 μMChembridge (Fragment library)6756840.09375TSC3-RPN4
0.0944.14E-13SGTC_24605552655 174.2 μMMiscellaneous54019890.298246amide catabolism
0.0936.16E-13SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.264151

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_8670518-0118155 μM0.5652176848158ChemDiv (Drug-like library)256.256681.40235DNA intercalators
SGTC_9383126-1755342 μM0.5416671777771ChemDiv (Drug-like library)390.388723.01235
SGTC_185k048-003725.49 μM0.4905666827589ChemDiv (Drug-like library)414.04882.30735RSC & ERG11
SGTC_11841269-1718139 μM0.4615385403821ChemDiv (Drug-like library)260.311582.6214
SGTC_11730443-0020122 μM0.445331295ChemDiv (Drug-like library)254.280563.44313TSC3-RPN4
SGTC_15101123-020071.5 μM0.3877555378812ChemDiv (Drug-like library)313.392365.39602
SGTC_155k072-020243.6 μM0.3859655857737ChemDiv (Drug-like library)332.352644.63825
SGTC_9781416-051287.4 μM0.3684216814003ChemDiv (Drug-like library)270.283260.69424redox potentiating
SGTC_1033k072-010221.3 μM0.3684215965931ChemDiv (Drug-like library)261.299642.1061660S ribosome export
SGTC_2481547894845.16 μM0.36538577792Miscellaneous270.279962.89614PDR1