2922-0838

3-(1H-benzo[f]benzimidazol-2-yl)aniline

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_538
Screen concentration 53.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 777039
SMILES C1=CC=C2C=C3C(=CC2=C1)NC(=N3)C4=CC(=CC=C4)N
Standardized SMILES Nc1cccc(c1)c2nc3cc4ccccc4cc3[nH]2
Molecular weight 259.3052
ALogP 3.43
H-bond donor count 2
H-bond acceptor count 2
Response signature Golgi

Pool Growth Kinetics
% growth inhibition (Het. pool) 20.46
% growth inhibition (Hom. pool) 11.8


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 777039
Download HIP data (tab-delimited text)  (excel)
Gene:CDC15(YAR019C)|FD-Score:-4.9|P-value:4.91E-7|Clearance:0||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:CUS1(YMR240C)|FD-Score:3.21|P-value:6.71E-4|Clearance:0.02||SGD DESC:Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p Gene:LSG1(YGL099W)|FD-Score:4.02|P-value:2.91E-5|Clearance:0.3||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM5(YER146W)|FD-Score:3.19|P-value:7.10E-4|Clearance:0.07||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:LST8(YNL006W)|FD-Score:5.28|P-value:6.38E-8|Clearance:1.06||SGD DESC:Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat Gene:MNP1(YGL068W)|FD-Score:3.37|P-value:3.74E-4|Clearance:0.02||SGD DESC:Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth Gene:NMD3(YHR170W)|FD-Score:4.21|P-value:1.27E-5|Clearance:0.19||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP4(YPL043W)|FD-Score:3.68|P-value:1.19E-4|Clearance:0.28||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:NRD1(YNL251C)|FD-Score:3.12|P-value:8.93E-4|Clearance:0.09||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites Gene:QNS1(YHR074W)|FD-Score:3.3|P-value:4.79E-4|Clearance:0.1||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RFC3(YNL290W)|FD-Score:3.72|P-value:1.00E-4|Clearance:0.04||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RKI1(YOR095C)|FD-Score:5.44|P-value:2.61E-8|Clearance:1.06||SGD DESC:Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis Gene:SEC31(YDL195W)|FD-Score:5.27|P-value:6.90E-8|Clearance:1.06||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:TIM10(YHR005C-A)|FD-Score:-3.22|P-value:6.52E-4|Clearance:0||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:VRG4(YGL225W)|FD-Score:3.35|P-value:4.05E-4|Clearance:0.05||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:YDR526C(YDR526C_d)|FD-Score:-3.81|P-value:7.04E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR198C(YLR198C_d)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:CDC15(YAR019C)|FD-Score:-4.9|P-value:4.91E-7|Clearance:0||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:CUS1(YMR240C)|FD-Score:3.21|P-value:6.71E-4|Clearance:0.02||SGD DESC:Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p Gene:LSG1(YGL099W)|FD-Score:4.02|P-value:2.91E-5|Clearance:0.3||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM5(YER146W)|FD-Score:3.19|P-value:7.10E-4|Clearance:0.07||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:LST8(YNL006W)|FD-Score:5.28|P-value:6.38E-8|Clearance:1.06||SGD DESC:Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat Gene:MNP1(YGL068W)|FD-Score:3.37|P-value:3.74E-4|Clearance:0.02||SGD DESC:Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth Gene:NMD3(YHR170W)|FD-Score:4.21|P-value:1.27E-5|Clearance:0.19||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP4(YPL043W)|FD-Score:3.68|P-value:1.19E-4|Clearance:0.28||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:NRD1(YNL251C)|FD-Score:3.12|P-value:8.93E-4|Clearance:0.09||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites Gene:QNS1(YHR074W)|FD-Score:3.3|P-value:4.79E-4|Clearance:0.1||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RFC3(YNL290W)|FD-Score:3.72|P-value:1.00E-4|Clearance:0.04||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RKI1(YOR095C)|FD-Score:5.44|P-value:2.61E-8|Clearance:1.06||SGD DESC:Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis Gene:SEC31(YDL195W)|FD-Score:5.27|P-value:6.90E-8|Clearance:1.06||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:TIM10(YHR005C-A)|FD-Score:-3.22|P-value:6.52E-4|Clearance:0||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:VRG4(YGL225W)|FD-Score:3.35|P-value:4.05E-4|Clearance:0.05||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:YDR526C(YDR526C_d)|FD-Score:-3.81|P-value:7.04E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR198C(YLR198C_d)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 777039
Download HOP data (tab-delimited text)  (excel)
Gene:AAC1(YMR056C)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:APA1(YCL050C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication Gene:ARL3(YPL051W)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ASG7(YJL170C)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Protein that regulates signaling from a G protein beta subunit Ste4p and its relocalization within the cell; specific to a-cells and induced by alpha-factor Gene:ATO2(YNR002C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication Gene:BNA1(YJR025C)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BOI2(YER114C)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication Gene:BPH1(YCR032W)|FD-Score:-3.25|P-value:5.69E-4||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:CLN3(YAL040C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:COG6(YNL041C)|FD-Score:4.32|P-value:7.89E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:6.52|P-value:3.57E-11||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:5.13|P-value:1.48E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL2(YIR029W)|FD-Score:-4.66|P-value:1.61E-6||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAL81(YIR023W)|FD-Score:5.42|P-value:3.03E-8||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:ECM5(YMR176W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress Gene:EHT1(YBR177C)|FD-Score:3.72|P-value:9.98E-5||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:ENT3(YJR125C)|FD-Score:5.29|P-value:6.21E-8||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p Gene:FPR2(YDR519W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:FZO1(YBR179C)|FD-Score:6.1|P-value:5.26E-10||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GET1(YGL020C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GMC1(YDR506C)|FD-Score:4.84|P-value:6.36E-7||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein Gene:HBT1(YDL223C)|FD-Score:-3.18|P-value:7.45E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HOM3(YER052C)|FD-Score:5.67|P-value:7.05E-9||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HXT5(YHR096C)|FD-Score:3.73|P-value:9.69E-5||SGD DESC:Hexose transporter with moderate affinity for glucose; induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs; HXT5 has a paralog, HXT3, that arose from the whole genome duplication Gene:IFA38(YBR159W)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:INO1(YJL153C)|FD-Score:4.56|P-value:2.51E-6||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:IRS4(YKR019C)|FD-Score:8.35|P-value:3.40E-17||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JJJ1(YNL227C)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis Gene:LIP2(YLR239C)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MBB1(YJL199C_d)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies Gene:MNN5(YJL186W)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRPL31(YKL138C)|FD-Score:3.09|P-value:0.00100||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NAT3(YPR131C)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met Gene:NVJ1(YHR195W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:PAU17(YLL025W)|FD-Score:3.76|P-value:8.50E-5||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene Gene:PHM8(YER037W)|FD-Score:4.52|P-value:3.09E-6||SGD DESC:Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p Gene:PMT5(YDL093W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals Gene:RAD51(YER095W)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RCR1(YBR005W)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication Gene:RKR1(YMR247C)|FD-Score:4.35|P-value:6.80E-6||SGD DESC:RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice Gene:RPA34(YJL148W)|FD-Score:8.51|P-value:8.75E-18||SGD DESC:RNA polymerase I subunit A34.5 Gene:RPA49(YNL248C)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:RNA polymerase I subunit A49 Gene:RPL11B(YGR085C)|FD-Score:3.9|P-value:4.79E-5||SGD DESC:Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication Gene:RPL21B(YPL079W)|FD-Score:4.92|P-value:4.43E-7||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPS10B(YMR230W)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:RRT14(YIL127C_p)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:RTC2(YBR147W)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:SBH1(YER087C-B)|FD-Score:4.34|P-value:7.15E-6||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SIL1(YOL031C)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SKI8(YGL213C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:STE23(YLR389C)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family Gene:STP1(YDR463W)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SYS1(YJL004C)|FD-Score:7.2|P-value:2.99E-13||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAL1(YLR354C)|FD-Score:3.75|P-value:8.72E-5||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:TAT1(YBR069C)|FD-Score:6.48|P-value:4.64E-11||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TLG2(YOL018C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA20(YER007C-A)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress Gene:TOS6(YNL300W_p)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TUL1(YKL034W)|FD-Score:-4.07|P-value:2.39E-5||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:ULA1(YPL003W)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation Gene:VMA7(YGR020C)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS63(YLR261C_d)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:WSC4(YHL028W)|FD-Score:4.71|P-value:1.27E-6||SGD DESC:ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 Gene:YAK1(YJL141C)|FD-Score:4.44|P-value:4.56E-6||SGD DESC:Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose Gene:YBL029C-A(YBL029C-A_p)|FD-Score:4.41|P-value:5.10E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica Gene:YBL059W(YBL059W_p)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YBR206W(YBR206W_d)|FD-Score:-3.58|P-value:1.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 Gene:YCL042W(YCL042W_p)|FD-Score:-3.27|P-value:5.36E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YCR090C(YCR090C_p)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YDL011C(YDL011C_d)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YDR379C-A(YDR379C-A)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YFL051C(YFL051C_p)|FD-Score:5.87|P-value:2.19E-9||SGD DESC:Putative protein of unknown function; YFL051C is not an essential gene Gene:YGR151C(YGR151C_d)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YIL152W(YIL152W_p)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Putative protein of unknown function Gene:YJL127C-B(YJL127C-B_p)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:Putative protein of unknown function; identified based on homology to the filamentous fungus, <i>Ashbya gossypii</i> Gene:YLR171W(YLR171W_d)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML002W(YML002W_p)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YML122C(YML122C_d)|FD-Score:-3.23|P-value:6.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR173W-A(YMR173W-A_d)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YMR254C(YMR254C_d)|FD-Score:-3.81|P-value:6.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR310C(YMR310C_p)|FD-Score:4.55|P-value:2.70E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNL010W(YNL010W_p)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YOL014W(YOL014W_p)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Putative protein of unknown function Gene:YPR071W(YPR071W_p)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Putative membrane protein; YPR071W is not an essential gene; YPR071W has paralog YIL029C Gene:YPR077C(YPR077C_d)|FD-Score:4.34|P-value:7.22E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YSC83(YHR017W)|FD-Score:5.28|P-value:6.45E-8||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:AAC1(YMR056C)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:APA1(YCL050C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication Gene:ARL3(YPL051W)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ASG7(YJL170C)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Protein that regulates signaling from a G protein beta subunit Ste4p and its relocalization within the cell; specific to a-cells and induced by alpha-factor Gene:ATO2(YNR002C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication Gene:BNA1(YJR025C)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BOI2(YER114C)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication Gene:BPH1(YCR032W)|FD-Score:-3.25|P-value:5.69E-4||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:CLN3(YAL040C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:COG6(YNL041C)|FD-Score:4.32|P-value:7.89E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:6.52|P-value:3.57E-11||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:5.13|P-value:1.48E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL2(YIR029W)|FD-Score:-4.66|P-value:1.61E-6||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAL81(YIR023W)|FD-Score:5.42|P-value:3.03E-8||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:ECM5(YMR176W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress Gene:EHT1(YBR177C)|FD-Score:3.72|P-value:9.98E-5||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:ENT3(YJR125C)|FD-Score:5.29|P-value:6.21E-8||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p Gene:FPR2(YDR519W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:FZO1(YBR179C)|FD-Score:6.1|P-value:5.26E-10||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GET1(YGL020C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GMC1(YDR506C)|FD-Score:4.84|P-value:6.36E-7||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein Gene:HBT1(YDL223C)|FD-Score:-3.18|P-value:7.45E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HOM3(YER052C)|FD-Score:5.67|P-value:7.05E-9||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HXT5(YHR096C)|FD-Score:3.73|P-value:9.69E-5||SGD DESC:Hexose transporter with moderate affinity for glucose; induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs; HXT5 has a paralog, HXT3, that arose from the whole genome duplication Gene:IFA38(YBR159W)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:INO1(YJL153C)|FD-Score:4.56|P-value:2.51E-6||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:IRS4(YKR019C)|FD-Score:8.35|P-value:3.40E-17||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JJJ1(YNL227C)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis Gene:LIP2(YLR239C)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MBB1(YJL199C_d)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies Gene:MNN5(YJL186W)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRPL31(YKL138C)|FD-Score:3.09|P-value:0.00100||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NAT3(YPR131C)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met Gene:NVJ1(YHR195W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:PAU17(YLL025W)|FD-Score:3.76|P-value:8.50E-5||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene Gene:PHM8(YER037W)|FD-Score:4.52|P-value:3.09E-6||SGD DESC:Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p Gene:PMT5(YDL093W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals Gene:RAD51(YER095W)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RCR1(YBR005W)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication Gene:RKR1(YMR247C)|FD-Score:4.35|P-value:6.80E-6||SGD DESC:RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice Gene:RPA34(YJL148W)|FD-Score:8.51|P-value:8.75E-18||SGD DESC:RNA polymerase I subunit A34.5 Gene:RPA49(YNL248C)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:RNA polymerase I subunit A49 Gene:RPL11B(YGR085C)|FD-Score:3.9|P-value:4.79E-5||SGD DESC:Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication Gene:RPL21B(YPL079W)|FD-Score:4.92|P-value:4.43E-7||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPS10B(YMR230W)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:RRT14(YIL127C_p)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:RTC2(YBR147W)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:SBH1(YER087C-B)|FD-Score:4.34|P-value:7.15E-6||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SIL1(YOL031C)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SKI8(YGL213C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:STE23(YLR389C)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family Gene:STP1(YDR463W)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SYS1(YJL004C)|FD-Score:7.2|P-value:2.99E-13||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAL1(YLR354C)|FD-Score:3.75|P-value:8.72E-5||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:TAT1(YBR069C)|FD-Score:6.48|P-value:4.64E-11||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TLG2(YOL018C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA20(YER007C-A)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress Gene:TOS6(YNL300W_p)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TUL1(YKL034W)|FD-Score:-4.07|P-value:2.39E-5||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:ULA1(YPL003W)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation Gene:VMA7(YGR020C)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS63(YLR261C_d)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:WSC4(YHL028W)|FD-Score:4.71|P-value:1.27E-6||SGD DESC:ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 Gene:YAK1(YJL141C)|FD-Score:4.44|P-value:4.56E-6||SGD DESC:Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose Gene:YBL029C-A(YBL029C-A_p)|FD-Score:4.41|P-value:5.10E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica Gene:YBL059W(YBL059W_p)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YBR206W(YBR206W_d)|FD-Score:-3.58|P-value:1.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 Gene:YCL042W(YCL042W_p)|FD-Score:-3.27|P-value:5.36E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YCR090C(YCR090C_p)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YDL011C(YDL011C_d)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YDR379C-A(YDR379C-A)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YFL051C(YFL051C_p)|FD-Score:5.87|P-value:2.19E-9||SGD DESC:Putative protein of unknown function; YFL051C is not an essential gene Gene:YGR151C(YGR151C_d)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YIL152W(YIL152W_p)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Putative protein of unknown function Gene:YJL127C-B(YJL127C-B_p)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:Putative protein of unknown function; identified based on homology to the filamentous fungus, <i>Ashbya gossypii</i> Gene:YLR171W(YLR171W_d)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML002W(YML002W_p)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YML122C(YML122C_d)|FD-Score:-3.23|P-value:6.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR173W-A(YMR173W-A_d)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YMR254C(YMR254C_d)|FD-Score:-3.81|P-value:6.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR310C(YMR310C_p)|FD-Score:4.55|P-value:2.70E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNL010W(YNL010W_p)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YOL014W(YOL014W_p)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Putative protein of unknown function Gene:YPR071W(YPR071W_p)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Putative membrane protein; YPR071W is not an essential gene; YPR071W has paralog YIL029C Gene:YPR077C(YPR077C_d)|FD-Score:4.34|P-value:7.22E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YSC83(YHR017W)|FD-Score:5.28|P-value:6.45E-8||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR095C5.442.61E-81.06RKI1Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis
YNL006W5.286.38E-81.06LST8Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat
YDL195W5.276.90E-81.06SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YHR170W4.211.27E-50.19NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YGL099W4.022.91E-50.30LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YNL290W3.721.00E-40.04RFC3Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YPL043W3.681.19E-40.28NOP4Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
YLR198C_d3.393.45E-40.02YLR198C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W
YGL068W3.373.74E-40.02MNP1Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth
YGL225W3.354.05E-40.05VRG4Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi
YHR074W3.304.79E-40.10QNS1Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide
YMR240C3.216.71E-40.02CUS1Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p
YER146W3.197.10E-40.07LSM5Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
YNL251C3.128.93E-40.09NRD1RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites
YGL233W3.030.001200.10SEC15Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL148W8.518.75E-18RPA34RNA polymerase I subunit A34.5
YKR019C8.353.40E-17IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YJL004C7.202.99E-13SYS1Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
YGL005C6.523.57E-11COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YBR069C6.484.64E-11TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YBR179C6.105.26E-10FZO1Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system
YFL051C_p5.872.19E-9YFL051C_pPutative protein of unknown function; YFL051C is not an essential gene
YER052C5.677.05E-9HOM3Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
YIR023W5.423.03E-8DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YJR125C5.296.21E-8ENT3Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p
YHR017W5.286.45E-8YSC83Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83
YDR463W5.191.03E-7STP1Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication
YML071C5.131.48E-7COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YPL079W4.924.43E-7RPL21BRibosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication
YDR506C4.846.36E-7GMC1Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein

GO enrichment analysis for SGTC_538
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1911.65E-49SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.140845Golgi
0.1883.14E-48SGTC_6051315-0399 119.0 μMChemDiv (Drug-like library)240204610.142857Golgi
0.1879.48E-48SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.140351Golgi
0.1761.80E-42SGTC_10603448-7350 82.0 μMChemDiv (Drug-like library)5701780.0862069Golgi
0.1742.88E-41SGTC_24605552655 174.2 μMMiscellaneous54019890.0952381amide catabolism
0.1666.43E-38SGTC_1078idebenone 36.1 μMMiscellaneous36860.0163934Golgi
0.1632.36E-36SGTC_31019120531 49.5 μMChembridge (Drug-like library)248249040.132353
0.1632.39E-36SGTC_389tpck 16.7 μMMiscellaneous4396470.0746269
0.1604.68E-35SGTC_1985st071382 65.5 μMTimTec (Natural product derivative library)61232370.0434783
0.1491.27E-30SGTC_910417-1751 58.4 μMChemDiv (Drug-like library)67956240.176471Golgi
0.1475.33E-30SGTC_2737bleomycin 30.5 nMMiscellaneous162113180.0414201Golgi
0.1461.23E-29SGTC_22557941087 197.4 μMChembridge (Fragment library)9355610.0923077
0.1461.29E-29SGTC_20415107442 45.3 μMChembridge (Fragment library)7930050.0727273RSC complex & mRNA processing
0.1452.62E-29SGTC_7171532-0246 73.2 μMChemDiv (Drug-like library)95619920.183673Golgi
0.1425.96E-28SGTC_15324',5'-dihydroxyflavone 78.7 μMTimTec (Pure natural product library)1655210.101695Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_8380109-020371.4 μM0.44186759422ChemDiv (Drug-like library)208.258483.75611
SGTC_6990375-0592111 μM0.395833762578ChemDiv (Drug-like library)251.326283.71822iron homeostasis
SGTC_660109-018742.4 μM0.363636759417ChemDiv (Drug-like library)228.676963.93511
SGTC_7800109-0199192 μM0.355556610414ChemDiv (Drug-like library)208.258483.75611
SGTC_6200109-0052432 μM0.345455493037ChemDiv (Drug-like library)462.502583.34655
SGTC_1655st01254655.1 μM0.294118720985TimTec (Natural product derivative library)237.253342.63113
SGTC_20464110841106 μM0.27272744119630Chembridge (Fragment library)242.294760.89334iron homeostasis
SGTC_21285255449168.49 μM0.264151736008Chembridge (Fragment library)211.219361.38314
SGTC_20084012253172 μM0.26087603940Chembridge (Fragment library)185.225142.05513
SGTC_1958st07651341.9 μM0.254545732122TimTec (Natural product derivative library)262.26283.31360S ribosome export