0159-0055

5-bromo-3-[2-(2,4,6-triphenylpyridin-1-ium-1-yl)ethyl]-1H-indole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_54
Screen concentration 9.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 3319099
SMILES C1=CC=C(C=C1)C2=CC(=[N+](C(=C2)C3=CC=CC=C3)CCC4=CNC5=C4C=C(C=C5)Br)C6=CC=CC=C6
Standardized SMILES Brc1ccc2[nH]cc(CC[n+]3c(cc(cc3c4ccccc4)c5ccccc5)c6ccccc6)c2c1
Molecular weight 530.4769
ALogP 9.52
H-bond donor count 1
H-bond acceptor count 0
Response signature ERG2

Pool Growth Kinetics
% growth inhibition (Het. pool) 18.24
% growth inhibition (Hom. pool) 8.22


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3319099
Download HIP data (tab-delimited text)  (excel)
Gene:ALA1(YOR335C)|FD-Score:-3.39|P-value:3.53E-4|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:CDC12(YHR107C)|FD-Score:5.84|P-value:2.68E-9|Clearance:0.71||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:FIP1(YJR093C)|FD-Score:5.22|P-value:9.10E-8|Clearance:0.71||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:GLC7(YER133W)|FD-Score:5.38|P-value:3.75E-8|Clearance:0.71||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:MCM7(YBR202W)|FD-Score:3.53|P-value:2.05E-4|Clearance:0.17||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MPE1(YKL059C)|FD-Score:3.11|P-value:9.38E-4|Clearance:0.18||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:NOP56(YLR197W)|FD-Score:3.86|P-value:5.56E-5|Clearance:0.33||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PAB1(YER165W)|FD-Score:3.12|P-value:8.95E-4|Clearance:0.01||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:PRE7(YBL041W)|FD-Score:4.87|P-value:5.52E-7|Clearance:0.71||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:RNA14(YMR061W)|FD-Score:3.36|P-value:3.90E-4|Clearance:0.04||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPA43(YOR340C)|FD-Score:-6.82|P-value:4.44E-12|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RPB7(YDR404C)|FD-Score:5.36|P-value:4.08E-8|Clearance:0.71||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPP1(YHR062C)|FD-Score:3.18|P-value:7.42E-4|Clearance:0.05||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SPC110(YDR356W)|FD-Score:-3.82|P-value:6.74E-5|Clearance:0||SGD DESC:Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner Gene:TEL2(YGR099W)|FD-Score:-3.14|P-value:8.46E-4|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TIM12(YBR091C)|FD-Score:4.58|P-value:2.33E-6|Clearance:0.71||SGD DESC:Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins Gene:UBC1(YDR177W)|FD-Score:-3.11|P-value:9.21E-4|Clearance:0||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:YJL086C(YJL086C_d)|FD-Score:-3.13|P-value:8.84E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YML6(YML025C)|FD-Score:3.32|P-value:4.58E-4|Clearance:0.14||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins Gene:YMR290W-A(YMR290W-A_d)|FD-Score:5.61|P-value:1.01E-8|Clearance:0.71||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:ALA1(YOR335C)|FD-Score:-3.39|P-value:3.53E-4|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:CDC12(YHR107C)|FD-Score:5.84|P-value:2.68E-9|Clearance:0.71||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:FIP1(YJR093C)|FD-Score:5.22|P-value:9.10E-8|Clearance:0.71||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:GLC7(YER133W)|FD-Score:5.38|P-value:3.75E-8|Clearance:0.71||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:MCM7(YBR202W)|FD-Score:3.53|P-value:2.05E-4|Clearance:0.17||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MPE1(YKL059C)|FD-Score:3.11|P-value:9.38E-4|Clearance:0.18||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:NOP56(YLR197W)|FD-Score:3.86|P-value:5.56E-5|Clearance:0.33||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PAB1(YER165W)|FD-Score:3.12|P-value:8.95E-4|Clearance:0.01||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:PRE7(YBL041W)|FD-Score:4.87|P-value:5.52E-7|Clearance:0.71||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:RNA14(YMR061W)|FD-Score:3.36|P-value:3.90E-4|Clearance:0.04||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPA43(YOR340C)|FD-Score:-6.82|P-value:4.44E-12|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RPB7(YDR404C)|FD-Score:5.36|P-value:4.08E-8|Clearance:0.71||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPP1(YHR062C)|FD-Score:3.18|P-value:7.42E-4|Clearance:0.05||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SPC110(YDR356W)|FD-Score:-3.82|P-value:6.74E-5|Clearance:0||SGD DESC:Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner Gene:TEL2(YGR099W)|FD-Score:-3.14|P-value:8.46E-4|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TIM12(YBR091C)|FD-Score:4.58|P-value:2.33E-6|Clearance:0.71||SGD DESC:Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins Gene:UBC1(YDR177W)|FD-Score:-3.11|P-value:9.21E-4|Clearance:0||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:YJL086C(YJL086C_d)|FD-Score:-3.13|P-value:8.84E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YML6(YML025C)|FD-Score:3.32|P-value:4.58E-4|Clearance:0.14||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins Gene:YMR290W-A(YMR290W-A_d)|FD-Score:5.61|P-value:1.01E-8|Clearance:0.71||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3319099
Download HOP data (tab-delimited text)  (excel)
Gene:AFG3(YER017C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:ALG12(YNR030W)|FD-Score:4.67|P-value:1.50E-6||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:ARG1(YOL058W)|FD-Score:14.5|P-value:4.30E-48||SGD DESC:Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate Gene:ARG2(YJL071W)|FD-Score:5.75|P-value:4.37E-9||SGD DESC:Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p Gene:ARG3(YJL088W)|FD-Score:14.3|P-value:1.28E-46||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARG4(YHR018C)|FD-Score:9.47|P-value:1.46E-21||SGD DESC:Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway Gene:ARG5,6(YER069W)|FD-Score:4.77|P-value:9.04E-7||SGD DESC:Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine Gene:ARG7(YMR062C)|FD-Score:4.64|P-value:1.75E-6||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ARG8(YOL140W)|FD-Score:12|P-value:2.52E-33||SGD DESC:Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine Gene:AVT1(YJR001W)|FD-Score:-3.82|P-value:6.78E-5||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BNI1(YNL271C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:COG7(YGL005C)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPA1(YOR303W)|FD-Score:7|P-value:1.31E-12||SGD DESC:Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader Gene:CPA2(YJR109C)|FD-Score:5.21|P-value:9.66E-8||SGD DESC:Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor Gene:CTK3(YML112W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CYK3(YDL117W)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants Gene:DCS1(YLR270W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DIA3(YDL024C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:EFG1(YGR271C-A)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ERG2(YMR202W)|FD-Score:29.8|P-value:1.08E-195||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:11.4|P-value:1.50E-30||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FSH3(YOR280C)|FD-Score:-3.81|P-value:7.04E-5||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:GAL11(YOL051W)|FD-Score:-3.21|P-value:6.64E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GCN4(YEL009C)|FD-Score:15.8|P-value:1.28E-56||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GEF1(YJR040W)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:GGC1(YDL198C)|FD-Score:4.64|P-value:1.72E-6||SGD DESC:Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family Gene:GLY1(YEL046C)|FD-Score:-4.03|P-value:2.82E-5||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GPM2(YDL021W)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:HAL5(YJL165C)|FD-Score:4.83|P-value:6.89E-7||SGD DESC:Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication Gene:IBA57(YJR122W)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IDP1(YDL066W)|FD-Score:6.33|P-value:1.19E-10||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IES2(YNL215W)|FD-Score:-3.88|P-value:5.20E-5||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:IMP2'(YIL154C)|FD-Score:23.1|P-value:4.97E-118||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC3(YDR332W)|FD-Score:5.28|P-value:6.53E-8||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:ISA2(YPR067W)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations Gene:ISW1(YBR245C)|FD-Score:5.34|P-value:4.74E-8||SGD DESC:ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions Gene:LAT1(YNL071W)|FD-Score:5.05|P-value:2.22E-7||SGD DESC:Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA Gene:LEM3(YNL323W)|FD-Score:12.5|P-value:2.49E-36||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LIP2(YLR239C)|FD-Score:8.18|P-value:1.46E-16||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:LIP5(YOR196C)|FD-Score:8.17|P-value:1.58E-16||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LPD1(YFL018C)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes Gene:MAL33(YBR297W)|FD-Score:-3.61|P-value:1.56E-4||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MOH1(YBL049W)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MOS1(YCL057C-A)|FD-Score:7.22|P-value:2.63E-13||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MRPL6(YHR147C)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB4(YOL112W)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:MSR1(YHR091C)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6 Gene:MSW1(YDR268W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MTM1(YGR257C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:NIP100(YPL174C)|FD-Score:-4.83|P-value:7.00E-7||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NMA111(YNL123W)|FD-Score:-6.27|P-value:1.85E-10||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:ORT1(YOR130C)|FD-Score:6.7|P-value:1.06E-11||SGD DESC:Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome Gene:OSH2(YDL019C)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PCL1(YNL289W)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PDA1(YER178W)|FD-Score:4.4|P-value:5.50E-6||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:PES4(YFR023W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PGD1(YGL025C)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PRO1(YDR300C)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:PRO2(YOR323C)|FD-Score:5.92|P-value:1.60E-9||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RCY1(YJL204C)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGL1(YPL066W)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RIC1(YLR039C)|FD-Score:-3.74|P-value:9.14E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RML2(YEL050C)|FD-Score:5.16|P-value:1.26E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RSA1(YPL193W)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RSC1(YGR056W)|FD-Score:5.51|P-value:1.84E-8||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SAC1(YKL212W)|FD-Score:11.9|P-value:5.99E-33||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SDC1(YDR469W)|FD-Score:4.56|P-value:2.53E-6||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SKT5(YBL061C)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SLG1(YOR008C)|FD-Score:-3.84|P-value:6.22E-5||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLM1(YIL105C)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:3.87|P-value:5.35E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STP22(YCL008C)|FD-Score:-4.3|P-value:8.63E-6||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUV3(YPL029W)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SWD3(YBR175W)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 Gene:TAE2(YPL009C)|FD-Score:-3.18|P-value:7.45E-4||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:THI3(YDL080C)|FD-Score:7.5|P-value:3.22E-14||SGD DESC:Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis Gene:TOM7(YNL070W)|FD-Score:4.4|P-value:5.50E-6||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRK1(YJL129C)|FD-Score:8.9|P-value:2.79E-19||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRM12(YML005W)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA Gene:WSS1(YHR134W)|FD-Score:-3.09|P-value:9.91E-4||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YAF9(YNL107W)|FD-Score:3.82|P-value:6.64E-5||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YCR085W(YCR085W_d)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL071C(YDL071C_d)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W Gene:YDR199W(YDR199W_d)|FD-Score:-3.77|P-value:8.02E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity Gene:YDR222W(YDR222W_p)|FD-Score:-4.38|P-value:5.96E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YDR340W(YDR340W_d)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Putative protein of unknown function Gene:YEF1(YEL041W)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YER068C-A(YER068C-A_d)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR153W(YGR153W_p)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Putative protein of unknown function Gene:YJL163C(YJL163C_p)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Putative protein of unknown function Gene:YJR061W(YJR061W_p)|FD-Score:-3.72|P-value:9.92E-5||SGD DESC:Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication Gene:YLR040C(YLR040C_p)|FD-Score:-4.31|P-value:8.27E-6||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YLR278C(YLR278C_p)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YML007C-A(YML007C-A_p)|FD-Score:7.71|P-value:6.34E-15||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML122C(YML122C_d)|FD-Score:-4.63|P-value:1.83E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR1(YGR281W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YOR200W(YOR200W_d)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPL025C(YPL025C_d)|FD-Score:-5.64|P-value:8.37E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR077C(YPR077C_d)|FD-Score:4.51|P-value:3.17E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPR097W(YPR097W)|FD-Score:5.84|P-value:2.55E-9||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YRR1(YOR162C)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ZUO1(YGR285C)|FD-Score:5.85|P-value:2.48E-9||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p Gene:AFG3(YER017C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:ALG12(YNR030W)|FD-Score:4.67|P-value:1.50E-6||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:ARG1(YOL058W)|FD-Score:14.5|P-value:4.30E-48||SGD DESC:Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate Gene:ARG2(YJL071W)|FD-Score:5.75|P-value:4.37E-9||SGD DESC:Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p Gene:ARG3(YJL088W)|FD-Score:14.3|P-value:1.28E-46||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARG4(YHR018C)|FD-Score:9.47|P-value:1.46E-21||SGD DESC:Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway Gene:ARG5,6(YER069W)|FD-Score:4.77|P-value:9.04E-7||SGD DESC:Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine Gene:ARG7(YMR062C)|FD-Score:4.64|P-value:1.75E-6||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ARG8(YOL140W)|FD-Score:12|P-value:2.52E-33||SGD DESC:Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine Gene:AVT1(YJR001W)|FD-Score:-3.82|P-value:6.78E-5||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BNI1(YNL271C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:COG7(YGL005C)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPA1(YOR303W)|FD-Score:7|P-value:1.31E-12||SGD DESC:Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader Gene:CPA2(YJR109C)|FD-Score:5.21|P-value:9.66E-8||SGD DESC:Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor Gene:CTK3(YML112W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CYK3(YDL117W)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants Gene:DCS1(YLR270W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DIA3(YDL024C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:EFG1(YGR271C-A)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ERG2(YMR202W)|FD-Score:29.8|P-value:1.08E-195||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:11.4|P-value:1.50E-30||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FSH3(YOR280C)|FD-Score:-3.81|P-value:7.04E-5||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:GAL11(YOL051W)|FD-Score:-3.21|P-value:6.64E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GCN4(YEL009C)|FD-Score:15.8|P-value:1.28E-56||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GEF1(YJR040W)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:GGC1(YDL198C)|FD-Score:4.64|P-value:1.72E-6||SGD DESC:Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family Gene:GLY1(YEL046C)|FD-Score:-4.03|P-value:2.82E-5||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GPM2(YDL021W)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:HAL5(YJL165C)|FD-Score:4.83|P-value:6.89E-7||SGD DESC:Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication Gene:IBA57(YJR122W)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IDP1(YDL066W)|FD-Score:6.33|P-value:1.19E-10||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IES2(YNL215W)|FD-Score:-3.88|P-value:5.20E-5||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:IMP2'(YIL154C)|FD-Score:23.1|P-value:4.97E-118||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC3(YDR332W)|FD-Score:5.28|P-value:6.53E-8||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:ISA2(YPR067W)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations Gene:ISW1(YBR245C)|FD-Score:5.34|P-value:4.74E-8||SGD DESC:ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions Gene:LAT1(YNL071W)|FD-Score:5.05|P-value:2.22E-7||SGD DESC:Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA Gene:LEM3(YNL323W)|FD-Score:12.5|P-value:2.49E-36||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LIP2(YLR239C)|FD-Score:8.18|P-value:1.46E-16||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:LIP5(YOR196C)|FD-Score:8.17|P-value:1.58E-16||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LPD1(YFL018C)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes Gene:MAL33(YBR297W)|FD-Score:-3.61|P-value:1.56E-4||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MOH1(YBL049W)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MOS1(YCL057C-A)|FD-Score:7.22|P-value:2.63E-13||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MRPL6(YHR147C)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB4(YOL112W)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:MSR1(YHR091C)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6 Gene:MSW1(YDR268W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MTM1(YGR257C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:NIP100(YPL174C)|FD-Score:-4.83|P-value:7.00E-7||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NMA111(YNL123W)|FD-Score:-6.27|P-value:1.85E-10||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:ORT1(YOR130C)|FD-Score:6.7|P-value:1.06E-11||SGD DESC:Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome Gene:OSH2(YDL019C)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PCL1(YNL289W)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PDA1(YER178W)|FD-Score:4.4|P-value:5.50E-6||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:PES4(YFR023W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PGD1(YGL025C)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PRO1(YDR300C)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:PRO2(YOR323C)|FD-Score:5.92|P-value:1.60E-9||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RCY1(YJL204C)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGL1(YPL066W)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RIC1(YLR039C)|FD-Score:-3.74|P-value:9.14E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RML2(YEL050C)|FD-Score:5.16|P-value:1.26E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RSA1(YPL193W)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RSC1(YGR056W)|FD-Score:5.51|P-value:1.84E-8||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SAC1(YKL212W)|FD-Score:11.9|P-value:5.99E-33||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SDC1(YDR469W)|FD-Score:4.56|P-value:2.53E-6||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SKT5(YBL061C)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SLG1(YOR008C)|FD-Score:-3.84|P-value:6.22E-5||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLM1(YIL105C)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:3.87|P-value:5.35E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STP22(YCL008C)|FD-Score:-4.3|P-value:8.63E-6||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUV3(YPL029W)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SWD3(YBR175W)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 Gene:TAE2(YPL009C)|FD-Score:-3.18|P-value:7.45E-4||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:THI3(YDL080C)|FD-Score:7.5|P-value:3.22E-14||SGD DESC:Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis Gene:TOM7(YNL070W)|FD-Score:4.4|P-value:5.50E-6||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRK1(YJL129C)|FD-Score:8.9|P-value:2.79E-19||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRM12(YML005W)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA Gene:WSS1(YHR134W)|FD-Score:-3.09|P-value:9.91E-4||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YAF9(YNL107W)|FD-Score:3.82|P-value:6.64E-5||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YCR085W(YCR085W_d)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL071C(YDL071C_d)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W Gene:YDR199W(YDR199W_d)|FD-Score:-3.77|P-value:8.02E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity Gene:YDR222W(YDR222W_p)|FD-Score:-4.38|P-value:5.96E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YDR340W(YDR340W_d)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Putative protein of unknown function Gene:YEF1(YEL041W)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YER068C-A(YER068C-A_d)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR153W(YGR153W_p)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Putative protein of unknown function Gene:YJL163C(YJL163C_p)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Putative protein of unknown function Gene:YJR061W(YJR061W_p)|FD-Score:-3.72|P-value:9.92E-5||SGD DESC:Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication Gene:YLR040C(YLR040C_p)|FD-Score:-4.31|P-value:8.27E-6||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YLR278C(YLR278C_p)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YML007C-A(YML007C-A_p)|FD-Score:7.71|P-value:6.34E-15||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML122C(YML122C_d)|FD-Score:-4.63|P-value:1.83E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR1(YGR281W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YOR200W(YOR200W_d)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPL025C(YPL025C_d)|FD-Score:-5.64|P-value:8.37E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR077C(YPR077C_d)|FD-Score:4.51|P-value:3.17E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPR097W(YPR097W)|FD-Score:5.84|P-value:2.55E-9||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YRR1(YOR162C)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ZUO1(YGR285C)|FD-Score:5.85|P-value:2.48E-9||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR107C5.842.68E-90.71CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YMR290W-A_d5.611.01E-80.71YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YER133W5.383.75E-80.71GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YDR404C5.364.08E-80.71RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
YJR093C5.229.10E-80.71FIP1Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p
YBL041W4.875.52E-70.71PRE7Beta 6 subunit of the 20S proteasome
YBR091C4.582.33E-60.71TIM12Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
YLR197W3.865.56E-50.33NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YBR202W3.532.05E-40.17MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YMR061W3.363.90E-40.04RNA14Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YML025C3.324.58E-40.14YML6Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins
YHR062C3.187.42E-40.05RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YER165W3.128.95E-40.01PAB1Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
YKL059C3.119.38E-40.18MPE1Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif
YAL038W2.930.001700.04CDC19Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR202W29.801.08E-195ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YIL154C23.104.97E-118IMP2'Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YEL009C15.801.28E-56GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YOL058W14.504.30E-48ARG1Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate
YJL088W14.301.28E-46ARG3Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine
YNL323W12.502.49E-36LEM3Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane
YOL140W12.002.52E-33ARG8Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine
YKL212W11.905.99E-33SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YGL012W11.401.50E-30ERG4C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
YHR018C9.471.46E-21ARG4Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway
YJL129C8.902.79E-19TRK1Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
YLR239C8.181.46E-16LIP2Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
YOR196C8.171.58E-16LIP5Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase
YML007C-A_p7.716.34E-15YML007C-A_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria
YDL080C7.503.22E-14THI3Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis

GO enrichment analysis for SGTC_54
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3937.41E-217SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.057971ERG2
0.3814.30E-203SGTC_2523spermine 47.7 μMMicrosource (Natural product library)11030.0188679calcium & mitochondrial duress
0.3746.57E-196SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.131579ERG2
0.3632.09E-183SGTC_400oxethazaine 37.5 μMMiscellaneous46210.1calcium & mitochondrial duress
0.3421.07E-161SGTC_1627st003713 25.6 μMTimTec (Natural product derivative library)161950700.0736842calcium & mitochondrial duress
0.3159.62E-136SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.231884ERG2
0.3052.92E-127SGTC_12470448-0043 5.8 μMChemDiv (Drug-like library)30890130.101449ERG2
0.2971.08E-120SGTC_1666st014829 29.0 μMTimTec (Natural product derivative library)38582300.0568182calcium & mitochondrial duress
0.2964.11E-120SGTC_1871488-2104 32.7 μMChemDiv (Drug-like library)28528590.101124calcium & mitochondrial duress
0.2962.03E-119SGTC_5820987-0078 156.0 μMChemDiv (Drug-like library)16257650.12calcium & mitochondrial duress
0.2951.66E-118SGTC_1622st003707 19.4 μMTimTec (Natural product derivative library)101408990.0851064calcium & mitochondrial duress
0.2931.82E-117SGTC_14504112-4260 37.6 μMChemDiv (Drug-like library)16331750.144737calcium & mitochondrial duress
0.2852.18E-110SGTC_1624st003710 6.2 μMTimTec (Natural product derivative library)242070330.119565calcium & mitochondrial duress
0.2846.19E-110SGTC_15977-benzyloxygramine 47.5 μMTimTec (Pure natural product library)2608070.19697calcium & mitochondrial duress
0.2849.14E-110SGTC_9943910-0338 138.0 μMChemDiv (Drug-like library)34794810.109756calcium & mitochondrial duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_60844-001319.84 μM0.751584900ChemDiv (Drug-like library)451.580888.7710
SGTC_1973st07027550.2 μM0.35593217118334TimTec (Natural product derivative library)314.809264.27922
SGTC_452diindolylmethane8 μM0.3191493071ICCB bioactive library246.306464.39120RNA processing & uracil transport
SGTC_3660181-02064.31 μM0.311111274874ChemDiv (Drug-like library)324.998684.8210Golgi
SGTC_2588indole-3-carbinol100 μM0.3061223712Microsource (Natural product library)147.173861.51821
SGTC_12630774-120864.8 μM0.288136906585ChemDiv (Drug-like library)304.342543.51612
SGTC_10995-nonyloxytryptamine2.85 μM0.28571423581825NIH Clinical Collection338.915245.52532
SGTC_1774st04837148.47 μM0.2786894242727TimTec (Natural product derivative library)318.415483.47412RNA processing & uracil transport
SGTC_1992st07364323.3 μM0.2727273161292TimTec (Natural product derivative library)352.8109633.80924mitochondrial processes
SGTC_2935903801629.96 μM0.2727276463195Chembridge (Drug-like library)296.3387833.92722