1187-0821

4-chloro-N-(2,2-dichloro-1-cyanoethenyl)benzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_540
Screen concentration 17.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 847520
SMILES C1=CC(=CC=C1C(=O)NC(=C(Cl)Cl)C#N)Cl
Standardized SMILES ClC(=C(NC(=O)c1ccc(Cl)cc1)C#N)Cl
Molecular weight 275.5185
ALogP 2.64
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 20.46
% growth inhibition (Hom. pool) 10.79


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 847520
Download HIP data (tab-delimited text)  (excel)
Gene:CCT5(YJR064W)|FD-Score:-5.13|P-value:1.47E-7|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:DSL1(YNL258C)|FD-Score:3.17|P-value:7.70E-4|Clearance:0.02||SGD DESC:Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene Gene:ESS1(YJR017C)|FD-Score:5.46|P-value:2.41E-8|Clearance:0.36||SGD DESC:Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3 Gene:GCD1(YOR260W)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.14||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCD2(YGR083C)|FD-Score:4.59|P-value:2.21E-6|Clearance:0.35||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPI14(YJR013W)|FD-Score:3.91|P-value:4.69E-5|Clearance:0.22||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:IMP3(YHR148W)|FD-Score:-4.22|P-value:1.21E-5|Clearance:0||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:MIA40(YKL195W)|FD-Score:5.28|P-value:6.43E-8|Clearance:0.36||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NIP1(YMR309C)|FD-Score:-3.56|P-value:1.86E-4|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOP7(YGR103W)|FD-Score:3.15|P-value:8.19E-4|Clearance:0.16||SGD DESC:Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G<sub>0</sub> and the initiation of cell proliferation Gene:NSL1(YPL233W)|FD-Score:3.55|P-value:1.91E-4|Clearance:0.02||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NUT2(YPR168W)|FD-Score:3.23|P-value:6.20E-4|Clearance:0.06||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress Gene:PRP45(YAL032C)|FD-Score:3.47|P-value:2.57E-4|Clearance:0.24||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:PUP2(YGR253C)|FD-Score:-3.18|P-value:7.27E-4|Clearance:0||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:RET1(YOR207C)|FD-Score:3.54|P-value:2.03E-4|Clearance:0.06||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPC37(YKR025W)|FD-Score:3.24|P-value:6.03E-4|Clearance:0.01||SGD DESC:RNA polymerase III subunit C37 Gene:RPS13(YDR064W)|FD-Score:-4.28|P-value:9.46E-6|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:SEC7(YDR170C)|FD-Score:3.15|P-value:8.11E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SPB4(YFL002C)|FD-Score:4.92|P-value:4.31E-7|Clearance:0.32||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:SSY1(YDR160W)|FD-Score:4.24|P-value:1.12E-5|Clearance:0.33||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:TID3(YIL144W)|FD-Score:4.6|P-value:2.09E-6|Clearance:0.01||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:CCT5(YJR064W)|FD-Score:-5.13|P-value:1.47E-7|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:DSL1(YNL258C)|FD-Score:3.17|P-value:7.70E-4|Clearance:0.02||SGD DESC:Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene Gene:ESS1(YJR017C)|FD-Score:5.46|P-value:2.41E-8|Clearance:0.36||SGD DESC:Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3 Gene:GCD1(YOR260W)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.14||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCD2(YGR083C)|FD-Score:4.59|P-value:2.21E-6|Clearance:0.35||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPI14(YJR013W)|FD-Score:3.91|P-value:4.69E-5|Clearance:0.22||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:IMP3(YHR148W)|FD-Score:-4.22|P-value:1.21E-5|Clearance:0||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:MIA40(YKL195W)|FD-Score:5.28|P-value:6.43E-8|Clearance:0.36||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NIP1(YMR309C)|FD-Score:-3.56|P-value:1.86E-4|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOP7(YGR103W)|FD-Score:3.15|P-value:8.19E-4|Clearance:0.16||SGD DESC:Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G<sub>0</sub> and the initiation of cell proliferation Gene:NSL1(YPL233W)|FD-Score:3.55|P-value:1.91E-4|Clearance:0.02||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NUT2(YPR168W)|FD-Score:3.23|P-value:6.20E-4|Clearance:0.06||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress Gene:PRP45(YAL032C)|FD-Score:3.47|P-value:2.57E-4|Clearance:0.24||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:PUP2(YGR253C)|FD-Score:-3.18|P-value:7.27E-4|Clearance:0||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:RET1(YOR207C)|FD-Score:3.54|P-value:2.03E-4|Clearance:0.06||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPC37(YKR025W)|FD-Score:3.24|P-value:6.03E-4|Clearance:0.01||SGD DESC:RNA polymerase III subunit C37 Gene:RPS13(YDR064W)|FD-Score:-4.28|P-value:9.46E-6|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:SEC7(YDR170C)|FD-Score:3.15|P-value:8.11E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SPB4(YFL002C)|FD-Score:4.92|P-value:4.31E-7|Clearance:0.32||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:SSY1(YDR160W)|FD-Score:4.24|P-value:1.12E-5|Clearance:0.33||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:TID3(YIL144W)|FD-Score:4.6|P-value:2.09E-6|Clearance:0.01||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 847520
Download HOP data (tab-delimited text)  (excel)
Gene:APE3(YBR286W)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:APL4(YPR029C)|FD-Score:-4.49|P-value:3.51E-6||SGD DESC:Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport Gene:ATG27(YJL178C)|FD-Score:-4.56|P-value:2.51E-6||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:BEM1(YBR200W)|FD-Score:-3.11|P-value:9.41E-4||SGD DESC:Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p Gene:BIO5(YNR056C)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CKA1(YIL035C)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CLA4(YNL298W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG6(YNL041C)|FD-Score:-3.11|P-value:9.47E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAK2(YFL053W)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DAL2(YIR029W)|FD-Score:5.66|P-value:7.77E-9||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DOT1(YDR440W)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:DUS4(YLR405W)|FD-Score:-4.11|P-value:1.95E-5||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:FAU1(YER183C)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FMP52(YER004W)|FD-Score:-3.26|P-value:5.63E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FZO1(YBR179C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GEA2(YEL022W)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GEX2(YKR106W)|FD-Score:5.23|P-value:8.56E-8||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GSY1(YFR015C)|FD-Score:-3.38|P-value:3.56E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GUT1(YHL032C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:GYP8(YFL027C)|FD-Score:-3.17|P-value:7.74E-4||SGD DESC:GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport Gene:HER1(YOR227W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Protein of unknown function required for proliferation or remodeling of the ER that is caused by overexpression of Hmg2p; may interact with ribosomes, based on co-purification experiments; HER1 has a paralog, GIP3, that arose from the whole genome duplication Gene:HGH1(YGR187C)|FD-Score:-3.27|P-value:5.41E-4||SGD DESC:Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress Gene:HTB2(YBL002W)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:IML2(YJL082W)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication Gene:IOC3(YFR013W)|FD-Score:5.95|P-value:1.36E-9||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:-6.12|P-value:4.79E-10||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ITR1(YDR497C)|FD-Score:-3.14|P-value:8.32E-4||SGD DESC:Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress Gene:MGT1(YDL200C)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MNN2(YBR015C)|FD-Score:6.22|P-value:2.47E-10||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:OCA5(YHL029C_p)|FD-Score:3.89|P-value:5.09E-5||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:ORM2(YLR350W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:PAU24(YBR301W)|FD-Score:-5.01|P-value:2.69E-7||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PEP4(YPL154C)|FD-Score:4.49|P-value:3.65E-6||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PFK1(YGR240C)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PIN4(YBL051C)|FD-Score:5.7|P-value:5.98E-9||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PPH22(YDL188C)|FD-Score:-4.05|P-value:2.55E-5||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication Gene:PRK1(YIL095W)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication Gene:PRR2(YDL214C)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance Gene:PSY1(YKL076C_d)|FD-Score:-3.57|P-value:1.82E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C Gene:PTC7(YHR076W)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:QCR7(YDR529C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RGC1(YPR115W)|FD-Score:-3.12|P-value:9.14E-4||SGD DESC:Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:-3.46|P-value:2.73E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RXT3(YDL076C)|FD-Score:-3.35|P-value:4.08E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SDH4(YDR178W)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SGS1(YMR190C)|FD-Score:4.83|P-value:6.96E-7||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SLX9(YGR081C)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SRL1(YOR247W)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:TIM11(YDR322C-A)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase Gene:TIR1(YER011W)|FD-Score:-3.88|P-value:5.16E-5||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking Gene:UTR1(YJR049C)|FD-Score:4.27|P-value:9.64E-6||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VPS3(YDR495C)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:YDR401W(YDR401W_d)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR018C(YGR018C_p)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W Gene:YIH1(YCR059C)|FD-Score:3.9|P-value:4.73E-5||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YJL215C(YJL215C_d)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR146W(YJR146W_p)|FD-Score:-3.39|P-value:3.49E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2 Gene:YKL133C(YKL133C_p)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YLL044W(YLL044W_d)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YLR269C(YLR269C_d)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR285C-A(YLR285C-A_p)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YML002W(YML002W_p)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YOL150C(YOL150C_d)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL225W(YPL225W)|FD-Score:4.31|P-value:8.19E-6||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPR011C(YPR011C_p)|FD-Score:-3.14|P-value:8.43E-4||SGD DESC:Putative transporter, member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR014C(YPR014C_d)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR050C(YPR050C_d)|FD-Score:-3.98|P-value:3.52E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPR084W(YPR084W_p)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Putative protein of unknown function Gene:YRR1(YOR162C)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:APE3(YBR286W)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:APL4(YPR029C)|FD-Score:-4.49|P-value:3.51E-6||SGD DESC:Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport Gene:ATG27(YJL178C)|FD-Score:-4.56|P-value:2.51E-6||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:BEM1(YBR200W)|FD-Score:-3.11|P-value:9.41E-4||SGD DESC:Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p Gene:BIO5(YNR056C)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CKA1(YIL035C)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CLA4(YNL298W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG6(YNL041C)|FD-Score:-3.11|P-value:9.47E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAK2(YFL053W)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DAL2(YIR029W)|FD-Score:5.66|P-value:7.77E-9||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DOT1(YDR440W)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:DUS4(YLR405W)|FD-Score:-4.11|P-value:1.95E-5||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:FAU1(YER183C)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FMP52(YER004W)|FD-Score:-3.26|P-value:5.63E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FZO1(YBR179C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GEA2(YEL022W)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GEX2(YKR106W)|FD-Score:5.23|P-value:8.56E-8||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GSY1(YFR015C)|FD-Score:-3.38|P-value:3.56E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GUT1(YHL032C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:GYP8(YFL027C)|FD-Score:-3.17|P-value:7.74E-4||SGD DESC:GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport Gene:HER1(YOR227W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Protein of unknown function required for proliferation or remodeling of the ER that is caused by overexpression of Hmg2p; may interact with ribosomes, based on co-purification experiments; HER1 has a paralog, GIP3, that arose from the whole genome duplication Gene:HGH1(YGR187C)|FD-Score:-3.27|P-value:5.41E-4||SGD DESC:Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress Gene:HTB2(YBL002W)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:IML2(YJL082W)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication Gene:IOC3(YFR013W)|FD-Score:5.95|P-value:1.36E-9||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:-6.12|P-value:4.79E-10||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ITR1(YDR497C)|FD-Score:-3.14|P-value:8.32E-4||SGD DESC:Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress Gene:MGT1(YDL200C)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MNN2(YBR015C)|FD-Score:6.22|P-value:2.47E-10||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:OCA5(YHL029C_p)|FD-Score:3.89|P-value:5.09E-5||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:ORM2(YLR350W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:PAU24(YBR301W)|FD-Score:-5.01|P-value:2.69E-7||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PEP4(YPL154C)|FD-Score:4.49|P-value:3.65E-6||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PFK1(YGR240C)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PIN4(YBL051C)|FD-Score:5.7|P-value:5.98E-9||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PPH22(YDL188C)|FD-Score:-4.05|P-value:2.55E-5||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication Gene:PRK1(YIL095W)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication Gene:PRR2(YDL214C)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance Gene:PSY1(YKL076C_d)|FD-Score:-3.57|P-value:1.82E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C Gene:PTC7(YHR076W)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:QCR7(YDR529C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RGC1(YPR115W)|FD-Score:-3.12|P-value:9.14E-4||SGD DESC:Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:-3.46|P-value:2.73E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RXT3(YDL076C)|FD-Score:-3.35|P-value:4.08E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SDH4(YDR178W)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SGS1(YMR190C)|FD-Score:4.83|P-value:6.96E-7||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SLX9(YGR081C)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SRL1(YOR247W)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:TIM11(YDR322C-A)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase Gene:TIR1(YER011W)|FD-Score:-3.88|P-value:5.16E-5||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking Gene:UTR1(YJR049C)|FD-Score:4.27|P-value:9.64E-6||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VPS3(YDR495C)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:YDR401W(YDR401W_d)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR018C(YGR018C_p)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W Gene:YIH1(YCR059C)|FD-Score:3.9|P-value:4.73E-5||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YJL215C(YJL215C_d)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR146W(YJR146W_p)|FD-Score:-3.39|P-value:3.49E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2 Gene:YKL133C(YKL133C_p)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YLL044W(YLL044W_d)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YLR269C(YLR269C_d)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR285C-A(YLR285C-A_p)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YML002W(YML002W_p)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YOL150C(YOL150C_d)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL225W(YPL225W)|FD-Score:4.31|P-value:8.19E-6||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPR011C(YPR011C_p)|FD-Score:-3.14|P-value:8.43E-4||SGD DESC:Putative transporter, member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR014C(YPR014C_d)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR050C(YPR050C_d)|FD-Score:-3.98|P-value:3.52E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPR084W(YPR084W_p)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Putative protein of unknown function Gene:YRR1(YOR162C)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YJR017C5.462.41E-80.36ESS1Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3
YKL195W5.286.43E-80.36MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YFL002C4.924.31E-70.32SPB4Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients
YIL144W4.602.09E-60.01TID3Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering
YGR083C4.592.21E-60.35GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YDR160W4.241.12E-50.33SSY1Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YJR013W3.914.69E-50.22GPI14Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
YOR260W3.691.12E-40.14GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YPL233W3.551.91E-40.02NSL1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation
YOR207C3.542.03E-40.06RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YAL032C3.472.57E-40.24PRP45Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
YKR025W3.246.03E-40.01RPC37RNA polymerase III subunit C37
YPR168W3.236.20E-40.06NUT2Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress
YNL258C3.177.70E-40.02DSL1Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene
YDR170C3.158.11E-40.00SEC7Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR015C6.222.47E-10MNN2Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
YFR013W5.951.36E-9IOC3Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication
YBL051C5.705.98E-9PIN4Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
YIR029W5.667.77E-9DAL2Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
YKR106W5.238.56E-8GEX2Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway
YMR190C4.836.96E-7SGS1Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress
YKL133C_p4.721.16E-6YKL133C_pPutative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype)
YPL154C4.493.65E-6PEP4Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates
YPL225W4.318.19E-6YPL225WProtein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
YJR049C4.279.64E-6UTR1ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication
YBR286W4.261.03E-5APE3Vacuolar aminopeptidase Y, processed to mature form by Prb1p
YOR227W4.141.77E-5HER1Protein of unknown function required for proliferation or remodeling of the ER that is caused by overexpression of Hmg2p; may interact with ribosomes, based on co-purification experiments; HER1 has a paralog, GIP3, that arose from the whole genome duplication
YNL298W4.141.77E-5CLA4Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p
YJL215C_d4.092.15E-5YJL215C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YER183C4.072.33E-5FAU15,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis

GO enrichment analysis for SGTC_540
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1285.53E-23SGTC_1771327-0068 8.4 μMChemDiv (Drug-like library)153899000.230769
0.0945.91E-13SGTC_2233sulbentine 40.4 μMMiscellaneous676860.0535714
0.0871.71E-11SGTC_392N-ethylmaleimide 8.3 μMMiscellaneous43620.0714286RNA pol III & RNase P/MRP
0.0863.32E-11SGTC_5941487-1584 10.8 μMChemDiv (Drug-like library)50133320.0895522
0.0856.28E-11SGTC_22236903751 107.6 μMChembridge (Fragment library)55988630.0606061superoxide
0.0831.63E-10SGTC_24305571206 200.0 μMMiscellaneous17317710.0597015
0.0791.49E-9SGTC_2510benzyl isothiocyanate 8.3 μMMicrosource (Natural product library)23460.0888889
0.0764.53E-9SGTC_2612dictamnine 15.5 μMMicrosource (Natural product library)680850.0535714RNA pol III & RNase P/MRP
0.0765.85E-9SGTC_1619st003326 88.7 μMTimTec (Natural product derivative library)28490320.087719360S ribosome export
0.0722.73E-8SGTC_3464513-0042 7.2 μMChemDiv (Drug-like library)28273720.115942azole & statin
0.0715.28E-8SGTC_2411st077133 39.9 μMTimTec (Natural product derivative library)14031420.0909091RNA pol III & RNase P/MRP
0.0715.48E-8SGTC_870100-0052 35.5 μMChemDiv (Drug-like library)2001870.170213
0.0707.02E-8SGTC_5394859-0462 2.5 μMChemDiv (Drug-like library)464994740.0735294RNA pol III & RNase P/MRP
0.0707.12E-8SGTC_6113453-1652 23.7 μMChemDiv (Drug-like library)15605750.0895522
0.0682.04E-7SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651800.0833333

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1400062-0056502.53 μM0.3902443758964ChemDiv (Drug-like library)302.969382.9622
SGTC_23639053361176 μM0.3555566469797Chembridge (Fragment library)260.718822.51212Golgi
SGTC_13131220-0047237 μM0.3513513528706ChemDiv (Drug-like library)231.67763.15212
SGTC_14780453-065635.7 μM0.2941185258496ChemDiv (Drug-like library)347.749762.81415
SGTC_3039909027049.47 μM0.29411817173750Chembridge (Drug-like library)303.740263.07113RPP1 & pyrimidine depletion
SGTC_23916285546200 μM0.291667846083Chembridge (Fragment library)208.644262.1612
SGTC_2471546695624.79 μM0.282609724419Miscellaneous274.699043.57713mitochondrial processes
SGTC_8340312-000875.7 μM0.279076385573ChemDiv (Drug-like library)210.660142.29613NEO1
SGTC_1726st03555614.58 μM0.2765965919439TimTec (Natural product derivative library)260.6907034.57102
SGTC_2872904403725.97 μM0.2765966465195Chembridge (Drug-like library)284.1131234.14912