1120-0019

5-chloro-1-ethyl-3-phenyl-2-[3-(1,3,3-trimethylindol-2-ylidene)prop-1-enyl]benzimidazol-1-ium

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_547
Screen concentration 610.0 nM
Source ChemDiv (Drug-like library)
PubChem CID 3676939
SMILES CC[N+]1=C(N(C2=C1C=CC(=C2)Cl)C3=CC=CC=C3)C=CC=C4C(C5=CC=CC=C5N4C)(C)C
Standardized SMILES CC[n+]1c(C=CC=C2N(C)c3ccccc3C2(C)C)n(c4ccccc4)c5cc(Cl)ccc15
Molecular weight 455.0137
ALogP 7.97
H-bond donor count 0
H-bond acceptor count 1
Response signature azole & statin

Pool Growth Kinetics
% growth inhibition (Het. pool) 62.54
% growth inhibition (Hom. pool) 7.31


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3676939
Download HIP data (tab-delimited text)  (excel)
Gene:CDC25(YLR310C)|FD-Score:-4.31|P-value:8.04E-6|Clearance:0||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:HEM15(YOR176W)|FD-Score:6.33|P-value:1.23E-10|Clearance:1.01||SGD DESC:Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway Gene:HEM3(YDL205C)|FD-Score:5.36|P-value:4.08E-8|Clearance:1.01||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:MCM5(YLR274W)|FD-Score:-4.82|P-value:7.14E-7|Clearance:0||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:MNP1(YGL068W)|FD-Score:3.6|P-value:1.61E-4|Clearance:0.06||SGD DESC:Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth Gene:NFS1(YCL017C)|FD-Score:-3.66|P-value:1.24E-4|Clearance:0||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:PRE1(YER012W)|FD-Score:-4.4|P-value:5.29E-6|Clearance:0||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PRE2(YPR103W)|FD-Score:-4|P-value:3.20E-5|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PRE4(YFR050C)|FD-Score:-4.95|P-value:3.78E-7|Clearance:0||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PRE5(YMR314W)|FD-Score:-4.71|P-value:1.23E-6|Clearance:0||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE6(YOL038W)|FD-Score:-4.36|P-value:6.36E-6|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRE7(YBL041W)|FD-Score:-4.67|P-value:1.51E-6|Clearance:0||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:PRE8(YML092C)|FD-Score:-5.08|P-value:1.87E-7|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PSE1(YMR308C)|FD-Score:-3.76|P-value:8.39E-5|Clearance:0||SGD DESC:Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p Gene:PTI1(YGR156W)|FD-Score:3.44|P-value:2.93E-4|Clearance:0.12||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:PUP2(YGR253C)|FD-Score:-4.09|P-value:2.15E-5|Clearance:0||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:PUP3(YER094C)|FD-Score:-3.89|P-value:4.99E-5|Clearance:0||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RAD3(YER171W)|FD-Score:-3.57|P-value:1.81E-4|Clearance:0||SGD DESC:5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress Gene:REB1(YBR049C)|FD-Score:3.31|P-value:4.61E-4|Clearance:0.13||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RPT2(YDL007W)|FD-Score:-3.74|P-value:9.05E-5|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle Gene:RPT3(YDR394W)|FD-Score:-5.29|P-value:6.00E-8|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT4(YOR259C)|FD-Score:-3.6|P-value:1.58E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RPT6(YGL048C)|FD-Score:-4.38|P-value:5.91E-6|Clearance:0||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RSC4(YKR008W)|FD-Score:5.04|P-value:2.27E-7|Clearance:1.01||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:SEC31(YDL195W)|FD-Score:-3.67|P-value:1.20E-4|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SGV1(YPR161C)|FD-Score:-4.06|P-value:2.41E-5|Clearance:0||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SLD3(YGL113W)|FD-Score:3.18|P-value:7.34E-4|Clearance:0.18||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SNM1(YDR478W)|FD-Score:-3.76|P-value:8.60E-5|Clearance:0||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:SRB4(YER022W)|FD-Score:-3.12|P-value:9.05E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:SRP72(YPL210C)|FD-Score:3.96|P-value:3.81E-5|Clearance:0.11||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:SUI1(YNL244C)|FD-Score:-3.95|P-value:3.90E-5|Clearance:0||SGD DESC:Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase Gene:SYF1(YDR416W)|FD-Score:4.97|P-value:3.37E-7|Clearance:1.01||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans Gene:TAF6(YGL112C)|FD-Score:7.07|P-value:7.91E-13|Clearance:1.01||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TAF8(YML114C)|FD-Score:6.3|P-value:1.46E-10|Clearance:1.01||SGD DESC:TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation Gene:TAF9(YMR236W)|FD-Score:3.53|P-value:2.05E-4|Clearance:0.09||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:TIM10(YHR005C-A)|FD-Score:-3.31|P-value:4.74E-4|Clearance:0||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TIM17(YJL143W)|FD-Score:-3.76|P-value:8.63E-5|Clearance:0||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TIM23(YNR017W)|FD-Score:-5.97|P-value:1.20E-9|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:TSR1(YDL060W)|FD-Score:-4.83|P-value:6.94E-7|Clearance:0||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UTP21(YLR409C)|FD-Score:3.85|P-value:5.98E-5|Clearance:0.25||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma Gene:YRB2(YIL063C)|FD-Score:-3.66|P-value:1.26E-4|Clearance:0||SGD DESC:Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential Gene:CDC25(YLR310C)|FD-Score:-4.31|P-value:8.04E-6|Clearance:0||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:HEM15(YOR176W)|FD-Score:6.33|P-value:1.23E-10|Clearance:1.01||SGD DESC:Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway Gene:HEM3(YDL205C)|FD-Score:5.36|P-value:4.08E-8|Clearance:1.01||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:MCM5(YLR274W)|FD-Score:-4.82|P-value:7.14E-7|Clearance:0||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:MNP1(YGL068W)|FD-Score:3.6|P-value:1.61E-4|Clearance:0.06||SGD DESC:Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth Gene:NFS1(YCL017C)|FD-Score:-3.66|P-value:1.24E-4|Clearance:0||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:PRE1(YER012W)|FD-Score:-4.4|P-value:5.29E-6|Clearance:0||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PRE2(YPR103W)|FD-Score:-4|P-value:3.20E-5|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PRE4(YFR050C)|FD-Score:-4.95|P-value:3.78E-7|Clearance:0||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PRE5(YMR314W)|FD-Score:-4.71|P-value:1.23E-6|Clearance:0||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE6(YOL038W)|FD-Score:-4.36|P-value:6.36E-6|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRE7(YBL041W)|FD-Score:-4.67|P-value:1.51E-6|Clearance:0||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:PRE8(YML092C)|FD-Score:-5.08|P-value:1.87E-7|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PSE1(YMR308C)|FD-Score:-3.76|P-value:8.39E-5|Clearance:0||SGD DESC:Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p Gene:PTI1(YGR156W)|FD-Score:3.44|P-value:2.93E-4|Clearance:0.12||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:PUP2(YGR253C)|FD-Score:-4.09|P-value:2.15E-5|Clearance:0||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:PUP3(YER094C)|FD-Score:-3.89|P-value:4.99E-5|Clearance:0||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RAD3(YER171W)|FD-Score:-3.57|P-value:1.81E-4|Clearance:0||SGD DESC:5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress Gene:REB1(YBR049C)|FD-Score:3.31|P-value:4.61E-4|Clearance:0.13||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RPT2(YDL007W)|FD-Score:-3.74|P-value:9.05E-5|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle Gene:RPT3(YDR394W)|FD-Score:-5.29|P-value:6.00E-8|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT4(YOR259C)|FD-Score:-3.6|P-value:1.58E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RPT6(YGL048C)|FD-Score:-4.38|P-value:5.91E-6|Clearance:0||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RSC4(YKR008W)|FD-Score:5.04|P-value:2.27E-7|Clearance:1.01||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:SEC31(YDL195W)|FD-Score:-3.67|P-value:1.20E-4|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SGV1(YPR161C)|FD-Score:-4.06|P-value:2.41E-5|Clearance:0||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SLD3(YGL113W)|FD-Score:3.18|P-value:7.34E-4|Clearance:0.18||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SNM1(YDR478W)|FD-Score:-3.76|P-value:8.60E-5|Clearance:0||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:SRB4(YER022W)|FD-Score:-3.12|P-value:9.05E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:SRP72(YPL210C)|FD-Score:3.96|P-value:3.81E-5|Clearance:0.11||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:SUI1(YNL244C)|FD-Score:-3.95|P-value:3.90E-5|Clearance:0||SGD DESC:Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase Gene:SYF1(YDR416W)|FD-Score:4.97|P-value:3.37E-7|Clearance:1.01||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans Gene:TAF6(YGL112C)|FD-Score:7.07|P-value:7.91E-13|Clearance:1.01||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TAF8(YML114C)|FD-Score:6.3|P-value:1.46E-10|Clearance:1.01||SGD DESC:TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation Gene:TAF9(YMR236W)|FD-Score:3.53|P-value:2.05E-4|Clearance:0.09||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:TIM10(YHR005C-A)|FD-Score:-3.31|P-value:4.74E-4|Clearance:0||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TIM17(YJL143W)|FD-Score:-3.76|P-value:8.63E-5|Clearance:0||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TIM23(YNR017W)|FD-Score:-5.97|P-value:1.20E-9|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:TSR1(YDL060W)|FD-Score:-4.83|P-value:6.94E-7|Clearance:0||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UTP21(YLR409C)|FD-Score:3.85|P-value:5.98E-5|Clearance:0.25||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma Gene:YRB2(YIL063C)|FD-Score:-3.66|P-value:1.26E-4|Clearance:0||SGD DESC:Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3676939
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARP6(YLR085C)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:ATP18(YML081C-A)|FD-Score:-4.71|P-value:1.24E-6||SGD DESC:Subunit of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms Gene:AZR1(YGR224W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole Gene:BMH1(YER177W)|FD-Score:4.05|P-value:2.56E-5||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CDA2(YLR308W)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Chitin deacetylase, together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:CDC50(YCR094W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:COQ9(YLR201C)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes Gene:COX14(YML129C)|FD-Score:-3.28|P-value:5.15E-4||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:CTK1(YKL139W)|FD-Score:7.37|P-value:8.29E-14||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DAP1(YPL170W)|FD-Score:6.96|P-value:1.68E-12||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DDR2(YOL052C-A)|FD-Score:-5.43|P-value:2.89E-8||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:ECM33(YBR078W)|FD-Score:3.91|P-value:4.55E-5||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ERG28(YER044C)|FD-Score:-3.11|P-value:9.50E-4||SGD DESC:Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p Gene:FIS1(YIL065C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:FMC1(YIL098C)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p Gene:FOB1(YDR110W)|FD-Score:-3.28|P-value:5.18E-4||SGD DESC:Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases Gene:GIM4(YEL003W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:HPM1(YIL110W)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:IMP2'(YIL154C)|FD-Score:3.9|P-value:4.89E-5||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:LIP5(YOR196C)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LSM1(YJL124C)|FD-Score:4.78|P-value:8.87E-7||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LYP1(YNL268W)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MGM101(YJR144W)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MIG1(YGL035C)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion Gene:MIP6(YHR015W)|FD-Score:4.93|P-value:4.02E-7||SGD DESC:Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication Gene:MNL2(YLR057W)|FD-Score:5.72|P-value:5.41E-9||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MRPL33(YMR286W)|FD-Score:-4.04|P-value:2.64E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NMD2(YHR077C)|FD-Score:-4.18|P-value:1.43E-5||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:NPR1(YNL183C)|FD-Score:4.93|P-value:4.01E-7||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:NUP120(YKL057C)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:OLA1(YBR025C)|FD-Score:-3.15|P-value:8.28E-4||SGD DESC:P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress Gene:PDX3(YBR035C)|FD-Score:7.35|P-value:1.02E-13||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:PEX6(YNL329C)|FD-Score:5.4|P-value:3.31E-8||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol Gene:PKH1(YDR490C)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:PSH1(YOL054W)|FD-Score:4.52|P-value:3.04E-6||SGD DESC:E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p Gene:QCR9(YGR183C)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex Gene:RAS1(YOR101W)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress Gene:RCY1(YJL204C)|FD-Score:6.51|P-value:3.80E-11||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RIM101(YHL027W)|FD-Score:6.32|P-value:1.31E-10||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM21(YNL294C)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RIM8(YGL045W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RNR4(YGR180C)|FD-Score:-4.03|P-value:2.84E-5||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPO41(YFL036W)|FD-Score:7.82|P-value:2.73E-15||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RPS17B(YDR447C)|FD-Score:-4.52|P-value:3.11E-6||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RTG1(YOL067C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTG2(YGL252C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:RTT10(YPL183C)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SAT4(YCR008W)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SER33(YIL074C)|FD-Score:3.88|P-value:5.27E-5||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SMI1(YGR229C)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SMM1(YNR015W)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs Gene:SSN2(YDR443C)|FD-Score:5.25|P-value:7.57E-8||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SSZ1(YHR064C)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:SWR1(YDR334W)|FD-Score:3.99|P-value:3.32E-5||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:SWT21(YNL187W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats Gene:TDH1(YJL052W)|FD-Score:3.8|P-value:7.31E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:THP2(YHR167W)|FD-Score:4.28|P-value:9.55E-6||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TMA22(YJR014W)|FD-Score:-4.46|P-value:4.00E-6||SGD DESC:Protein of unknown function; associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; similar to human GRAP and human DRP1, which interacts with human Tma20p homolog MCT-1; protein abundance increases in response to DNA replication stress Gene:TRE1(YPL176C)|FD-Score:4.05|P-value:2.56E-5||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRM9(YML014W)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:UBC7(YMR022W)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:UTR1(YJR049C)|FD-Score:7.84|P-value:2.29E-15||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VMA1(YDL185W)|FD-Score:-3.97|P-value:3.60E-5||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA13(YPR036W)|FD-Score:-3.11|P-value:9.32E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:WHI2(YOR043W)|FD-Score:3.74|P-value:9.13E-5||SGD DESC:Protein required, with binding partner Psr1p, for full activation of the general stress response, possibly through Msn2p dephosphorylation; regulates growth during the diauxic shift; negative regulator of G1 cyclin expression Gene:YDL011C(YDL011C_d)|FD-Score:-3.76|P-value:8.63E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YDL118W(YDL118W_p)|FD-Score:7.03|P-value:1.02E-12||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDL119C(YDL119C_p)|FD-Score:5.55|P-value:1.45E-8||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDR186C(YDR186C)|FD-Score:4.91|P-value:4.56E-7||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGL010W(YGL010W_p)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGL108C(YGL108C_p)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YGR122W(YGR122W)|FD-Score:4.57|P-value:2.39E-6||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YGR237C(YGR237C_p)|FD-Score:5.02|P-value:2.65E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHR045W(YHR045W_p)|FD-Score:5.07|P-value:1.97E-7||SGD DESC:Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:YHR097C(YHR097C_p)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YIL032C(YIL032C_d)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL169W(YJL169W_d)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YKL031W(YKL031W_d)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YKL068W-A(YKL068W-A_p)|FD-Score:-3.3|P-value:4.84E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLL032C(YLL032C)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene Gene:YNL067W-B(YNL067W-B_p)|FD-Score:-3.17|P-value:7.62E-4||SGD DESC:Putative protein of unknown function Gene:YNL211C(YNL211C_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YOL019W-A(YOL019W-A_p)|FD-Score:-3.74|P-value:9.37E-5||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YOR072W(YOR072W_d)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL229W(YPL229W_p)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:AFT1(YGL071W)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARP6(YLR085C)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:ATP18(YML081C-A)|FD-Score:-4.71|P-value:1.24E-6||SGD DESC:Subunit of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms Gene:AZR1(YGR224W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole Gene:BMH1(YER177W)|FD-Score:4.05|P-value:2.56E-5||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CDA2(YLR308W)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Chitin deacetylase, together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:CDC50(YCR094W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:COQ9(YLR201C)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes Gene:COX14(YML129C)|FD-Score:-3.28|P-value:5.15E-4||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:CTK1(YKL139W)|FD-Score:7.37|P-value:8.29E-14||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DAP1(YPL170W)|FD-Score:6.96|P-value:1.68E-12||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DDR2(YOL052C-A)|FD-Score:-5.43|P-value:2.89E-8||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:ECM33(YBR078W)|FD-Score:3.91|P-value:4.55E-5||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ERG28(YER044C)|FD-Score:-3.11|P-value:9.50E-4||SGD DESC:Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p Gene:FIS1(YIL065C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:FMC1(YIL098C)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p Gene:FOB1(YDR110W)|FD-Score:-3.28|P-value:5.18E-4||SGD DESC:Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases Gene:GIM4(YEL003W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:HPM1(YIL110W)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:IMP2'(YIL154C)|FD-Score:3.9|P-value:4.89E-5||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:LIP5(YOR196C)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LSM1(YJL124C)|FD-Score:4.78|P-value:8.87E-7||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LYP1(YNL268W)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MGM101(YJR144W)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MIG1(YGL035C)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion Gene:MIP6(YHR015W)|FD-Score:4.93|P-value:4.02E-7||SGD DESC:Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication Gene:MNL2(YLR057W)|FD-Score:5.72|P-value:5.41E-9||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MRPL33(YMR286W)|FD-Score:-4.04|P-value:2.64E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NMD2(YHR077C)|FD-Score:-4.18|P-value:1.43E-5||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:NPR1(YNL183C)|FD-Score:4.93|P-value:4.01E-7||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:NUP120(YKL057C)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:OLA1(YBR025C)|FD-Score:-3.15|P-value:8.28E-4||SGD DESC:P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress Gene:PDX3(YBR035C)|FD-Score:7.35|P-value:1.02E-13||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:PEX6(YNL329C)|FD-Score:5.4|P-value:3.31E-8||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol Gene:PKH1(YDR490C)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:PSH1(YOL054W)|FD-Score:4.52|P-value:3.04E-6||SGD DESC:E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p Gene:QCR9(YGR183C)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex Gene:RAS1(YOR101W)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress Gene:RCY1(YJL204C)|FD-Score:6.51|P-value:3.80E-11||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RIM101(YHL027W)|FD-Score:6.32|P-value:1.31E-10||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM21(YNL294C)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RIM8(YGL045W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RNR4(YGR180C)|FD-Score:-4.03|P-value:2.84E-5||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPO41(YFL036W)|FD-Score:7.82|P-value:2.73E-15||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RPS17B(YDR447C)|FD-Score:-4.52|P-value:3.11E-6||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RTG1(YOL067C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTG2(YGL252C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:RTT10(YPL183C)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SAT4(YCR008W)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SER33(YIL074C)|FD-Score:3.88|P-value:5.27E-5||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SMI1(YGR229C)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SMM1(YNR015W)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs Gene:SSN2(YDR443C)|FD-Score:5.25|P-value:7.57E-8||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SSZ1(YHR064C)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:SWR1(YDR334W)|FD-Score:3.99|P-value:3.32E-5||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:SWT21(YNL187W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats Gene:TDH1(YJL052W)|FD-Score:3.8|P-value:7.31E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:THP2(YHR167W)|FD-Score:4.28|P-value:9.55E-6||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TMA22(YJR014W)|FD-Score:-4.46|P-value:4.00E-6||SGD DESC:Protein of unknown function; associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; similar to human GRAP and human DRP1, which interacts with human Tma20p homolog MCT-1; protein abundance increases in response to DNA replication stress Gene:TRE1(YPL176C)|FD-Score:4.05|P-value:2.56E-5||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRM9(YML014W)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:UBC7(YMR022W)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:UTR1(YJR049C)|FD-Score:7.84|P-value:2.29E-15||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VMA1(YDL185W)|FD-Score:-3.97|P-value:3.60E-5||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA13(YPR036W)|FD-Score:-3.11|P-value:9.32E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:WHI2(YOR043W)|FD-Score:3.74|P-value:9.13E-5||SGD DESC:Protein required, with binding partner Psr1p, for full activation of the general stress response, possibly through Msn2p dephosphorylation; regulates growth during the diauxic shift; negative regulator of G1 cyclin expression Gene:YDL011C(YDL011C_d)|FD-Score:-3.76|P-value:8.63E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YDL118W(YDL118W_p)|FD-Score:7.03|P-value:1.02E-12||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDL119C(YDL119C_p)|FD-Score:5.55|P-value:1.45E-8||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDR186C(YDR186C)|FD-Score:4.91|P-value:4.56E-7||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGL010W(YGL010W_p)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGL108C(YGL108C_p)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YGR122W(YGR122W)|FD-Score:4.57|P-value:2.39E-6||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YGR237C(YGR237C_p)|FD-Score:5.02|P-value:2.65E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHR045W(YHR045W_p)|FD-Score:5.07|P-value:1.97E-7||SGD DESC:Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:YHR097C(YHR097C_p)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YIL032C(YIL032C_d)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL169W(YJL169W_d)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YKL031W(YKL031W_d)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YKL068W-A(YKL068W-A_p)|FD-Score:-3.3|P-value:4.84E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLL032C(YLL032C)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene Gene:YNL067W-B(YNL067W-B_p)|FD-Score:-3.17|P-value:7.62E-4||SGD DESC:Putative protein of unknown function Gene:YNL211C(YNL211C_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YOL019W-A(YOL019W-A_p)|FD-Score:-3.74|P-value:9.37E-5||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YOR072W(YOR072W_d)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL229W(YPL229W_p)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL112C7.077.91E-131.01TAF6Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4
YOR176W6.331.23E-101.01HEM15Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway
YML114C6.301.46E-101.01TAF8TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation
YDL205C5.364.08E-81.01HEM3Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
YKR008W5.042.27E-71.01RSC4Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p
YDR416W4.973.37E-71.01SYF1Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans
YPL210C3.963.81E-50.11SRP72Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane
YLR409C3.855.98E-50.25UTP21Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma
YGL068W3.601.61E-40.06MNP1Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth
YMR236W3.532.05E-40.09TAF9Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3
YGR156W3.442.93E-40.12PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
YBR049C3.314.61E-40.13REB1RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication
YGL113W3.187.34E-40.18SLD3Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress
YDR167W3.000.001340.04TAF10Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YLR132C2.960.001550.03USB1Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJR049C7.842.29E-15UTR1ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication
YFL036W7.822.73E-15RPO41Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
YKL139W7.378.29E-14CTK1Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
YBR035C7.351.02E-13PDX3Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism
YDL118W_p7.031.02E-12YDL118W_pDubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein
YPL170W6.961.68E-12DAP1Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis
YJL204C6.513.80E-11RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YHL027W6.321.31E-10RIM101Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC
YLR057W5.725.41E-9MNL2Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene
YDL119C_p5.551.45E-8YDL119C_pPutative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria
YNL329C5.403.31E-8PEX6AAA-peroxin that heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol
YDR443C5.257.57E-8SSN2Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation
YHR045W_p5.071.97E-7YHR045W_pPutative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum
YGR237C_p5.022.65E-7YGR237C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YNL183C4.934.01E-7NPR1Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex

GO enrichment analysis for SGTC_547
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3654.93E-186SGTC_9021120-0002 2.1 μMChemDiv (Drug-like library)32804750.868852azole & statin
0.3392.11E-158SGTC_3832324-0395 7.4 μMChemDiv (Drug-like library)31464210.168539azole & statin
0.2406.38E-78SGTC_13101188-0297 994.0 nMChemDiv (Drug-like library)15883520.188889azole & statin
0.2383.67E-77SGTC_29659082772 38.6 μMChembridge (Drug-like library)164596340.0851064mitochondrial response to ROS
0.2181.35E-64SGTC_13531498-1206 8.1 μMChemDiv (Drug-like library)67568080.0853659mitochondrial response to ROS
0.2142.40E-62SGTC_29259010536 32.5 μMChembridge (Drug-like library)29941770.0952381azole & statin
0.2126.72E-61SGTC_7783456-2434 196.0 μMChemDiv (Drug-like library)55390630.0823529mitochondrial response to ROS
0.1979.39E-53SGTC_13471493-0251 600.0 nMChemDiv (Drug-like library)39863780.0666667mitochondrial response to ROS
0.1831.03E-45SGTC_1788fluconazole 20.0 μMMiscellaneous33650.0697674azole & statin
0.1811.64E-44SGTC_1410443-0274 32.3 μMChemDiv (Drug-like library)16272410.0941176mitochondrial response to ROS
0.1779.83E-43SGTC_227fluconazole 33.5 μMMiscellaneous33650.0697674azole & statin
0.1764.89E-42SGTC_30629077494 49.5 μMChembridge (Drug-like library)164596330.0645161mitochondrial response to ROS
0.1701.97E-39SGTC_12400356-0016 42.1 μMChemDiv (Drug-like library)43056130.0769231mitochondrial response to ROS
0.1561.99E-33SGTC_217ketoconazole 989.0 nMMiscellaneous4562010.133929ergosterol depletion effects on membrane
0.1511.34E-31SGTC_11911487-1166 10.6 μMChemDiv (Drug-like library)39557710.108434azole & statin

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_9021120-00022.07 μM0.8688523280475ChemDiv (Drug-like library)455.013667.96501azole & statin
SGTC_1130099-02212.5 μM0.39436689219ChemDiv (Drug-like library)409.585726.64601
SGTC_13812324-006662.1 μM0.28915710885928ChemDiv (Drug-like library)402.293766.78503
SGTC_2919797818846.38 μM0.2439022980291Chembridge (Drug-like library)331.750362.26224
SGTC_396dithiazanine1.25 μM0.23255810578Miscellaneous518.476593.92904
SGTC_8560433-001611.3 μM0.2315793911780ChemDiv (Drug-like library)711.484362.93308
SGTC_3451362-0200395 μM0.2162161419823ChemDiv (Drug-like library)264.384684.45100
SGTC_1065prochlorperazine maleate2.48 μM0.2073176436057NIH Clinical Collection490.01482-1.33428
SGTC_2001402350257.66 μM0.202899108941Chembridge (Fragment library)195.602441.5402mitochondrial processes
SGTC_3014119-010024.5 μM0.2025322801ChemDiv (Drug-like library)314.852325.05202NEO1